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The map label for this gene is btuB [H]

Identifier: 114800171

GI number: 114800171

Start: 1153445

End: 1155346

Strand: Direct

Name: btuB [H]

Synonym: HNE_1142

Alternate gene names: 114800171

Gene position: 1153445-1155346 (Clockwise)

Preceding gene: 114798986

Following gene: 114799155

Centisome position: 31.13

GC content: 61.67

Gene sequence:

>1902_bases
ATGAACCCGCCGCGAGGGATCTCCCTCCGGCCAATGAAGGATGCTGTGGATATGTTCCTCAGAACCGCACTGCTGGGCGC
TTCGGCGCTCGTGATCACTGCTTCGTTTGCACAGGCAGAAGACACCCTCACTCTTGATCCTGTCGAAGTCAGCGGCCTGC
GCCCGATTGAATTCGACCGTCTGACTTCGTCTGTTTCTGTTCTGACGGCAGACGATCTTGCCATTCGTCTTTCCCCCAAT
CCGGCCGATCAGCTGCGCGCGGTTCCCGGCGTGGCGATCTCCCGTGCGGGCGGCGTCGGCGCGCTCACCCAGGTGCGCAT
GCGCGGCGCCGAAGCCAACCACACGCTGGTCCTGTTTGAAGGCATCGAGATTTCCGACCCCGTGAACGGGGATACTGATT
TCGGCCTGCTTACCGCGTTGCCCACCGGCCGTATCGAGGTGTTGCGCGGAGCGTCGTCCTCAATCCACGGATCAGACGCG
ATTGGCGGGGTCGTTTCCATTTCGGCTGCGCGCGAAGCGGGGCTGAATGCGTTTGCCGAGGCTGGCAGCTTCGGCACGGT
GCGCGGCGCACTGGGCCTGTCTGGCGAGAACTTTGGCGGGGCCATCACCGGGTTCACGACCGACGGTGTTGATACATCCG
GCACCGGCGGCGCCAAGGATGGGTCTGAAGCGCTCTCCGGCTTCGTTAACGGCAAGGTTGATCTTGGGGCGGATTGGTCG
CTCTCGCTGATCGGCCTCGCCCGCGGCATGGAATCGGAATTTGATTCGGATACGGATTTTGACGGCCTTCTCGACGATGA
AGACCGCCGCACCGAAGCAGACCAGTGGCTGGTGGGCGGCGCACTCAGCGGCGTAACCGGGCCGCTGGACCATGTCATCC
GCGCCAGCTTCAATGAAGTGAAGCGGGATAATTTCAGCGATGGCCAAAAGACGGATGTTTCCAAGGGCCAGCGCACCAAG
CTCGCCTGGTTGCCGAGCCTTGCCAATGGCGCGCATTCGCTGACCGGTATTCTCGACTATGAGCGTGAGGAGTATGATCG
CACGGATGTTCAGTATGCCGGCGCAACCGATGCTTCCGAGACGTTCGAGTCCATCGGCGTGGGCGCGGAGTACCGCTACT
CTGCCGGCGCGTTTGATGCATCCGCCTCGGCGCGGTTTGACGACAATGATGGCCGGTTTGATGATGCCACGACATGGCGT
CTCGGCGCAGGCTACGCATTTGAGCAGGTCGGCGGGCGGGTTCGCGCCTCGATCGGAACCGGGGTGAAAAACCCGACCTT
CACTGAACTGTTCGGCTTCTTCCCTGGCAGCTTCATCGGCAATCCCGACCTGAAGCCGGAAGAGTCCACCAGCTGGGAAA
TCGGCTGGGAACAGACGGTTGGACCGGTCAGCTATTCGGCCACATACTTTGAAGCCGATCTGGAGAACGAGATCTACACC
GCCTACAATCCCGACTTCACCTCGTCACCGGCCAACCGCGCCACGGACAGTGAGCGGAGCGGTGTAGAGCTTGCTGCGCG
CTGGCAGGCGCTCGCCAATCTGGCGTTCTTCGGCCAGGCAACCCTGTTTGACTCTCAGGACAATAACGGTGTGGACGAAA
TCCGGGTGCCTGAAGCAACCGCGTCCCTGGGGGCAGACTGGCTGGTCAATCCGAACGGGTTCCGGCTCGGCGCGGCGCTC
GATTATGTCGGCGAGCAGGGTGACACCGATTTCGGGCCGTTCCCGTCACGGCCTGTCAGCCTCGACGCCTACACGCTCGC
CAGCATCACCGCAGAATATCCGCTGACGGAGCGGATTGCGATCACGCTGCGGGGCCAGAATGTGTTCGATGAGAAGCCAA
CCGACATCTATGGCTATAATGGTCCGGGTGCTGGTGTATTCGTGGGACTGAAACTGAAGTGA

Upstream 100 bases:

>100_bases
ATCCGCGAAGTCAGGAGACCTGCCTGTTGCCGTCGTTGGCTCGCCGTCCGGGAACAGGCGTAGGGCCGCGGAAGGCCACC
TTCCGTTCATGCGACATGTC

Downstream 100 bases:

>100_bases
GACTGAGGCTTTCAGCCTTTTTCCTGATGGGACTGGCCGGTTTTGCTGTGGCAGAGCCGGCCAGCCTTACGCCTGCGCGG
CCGACGATCGTGTCACTGGA

Product: putative TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MNPPRGISLRPMKDAVDMFLRTALLGASALVITASFAQAEDTLTLDPVEVSGLRPIEFDRLTSSVSVLTADDLAIRLSPN
PADQLRAVPGVAISRAGGVGALTQVRMRGAEANHTLVLFEGIEISDPVNGDTDFGLLTALPTGRIEVLRGASSSIHGSDA
IGGVVSISAAREAGLNAFAEAGSFGTVRGALGLSGENFGGAITGFTTDGVDTSGTGGAKDGSEALSGFVNGKVDLGADWS
LSLIGLARGMESEFDSDTDFDGLLDDEDRRTEADQWLVGGALSGVTGPLDHVIRASFNEVKRDNFSDGQKTDVSKGQRTK
LAWLPSLANGAHSLTGILDYEREEYDRTDVQYAGATDASETFESIGVGAEYRYSAGAFDASASARFDDNDGRFDDATTWR
LGAGYAFEQVGGRVRASIGTGVKNPTFTELFGFFPGSFIGNPDLKPEESTSWEIGWEQTVGPVSYSATYFEADLENEIYT
AYNPDFTSSPANRATDSERSGVELAARWQALANLAFFGQATLFDSQDNNGVDEIRVPEATASLGADWLVNPNGFRLGAAL
DYVGEQGDTDFGPFPSRPVSLDAYTLASITAEYPLTERIAITLRGQNVFDEKPTDIYGYNGPGAGVFVGLKLK

Sequences:

>Translated_633_residues
MNPPRGISLRPMKDAVDMFLRTALLGASALVITASFAQAEDTLTLDPVEVSGLRPIEFDRLTSSVSVLTADDLAIRLSPN
PADQLRAVPGVAISRAGGVGALTQVRMRGAEANHTLVLFEGIEISDPVNGDTDFGLLTALPTGRIEVLRGASSSIHGSDA
IGGVVSISAAREAGLNAFAEAGSFGTVRGALGLSGENFGGAITGFTTDGVDTSGTGGAKDGSEALSGFVNGKVDLGADWS
LSLIGLARGMESEFDSDTDFDGLLDDEDRRTEADQWLVGGALSGVTGPLDHVIRASFNEVKRDNFSDGQKTDVSKGQRTK
LAWLPSLANGAHSLTGILDYEREEYDRTDVQYAGATDASETFESIGVGAEYRYSAGAFDASASARFDDNDGRFDDATTWR
LGAGYAFEQVGGRVRASIGTGVKNPTFTELFGFFPGSFIGNPDLKPEESTSWEIGWEQTVGPVSYSATYFEADLENEIYT
AYNPDFTSSPANRATDSERSGVELAARWQALANLAFFGQATLFDSQDNNGVDEIRVPEATASLGADWLVNPNGFRLGAAL
DYVGEQGDTDFGPFPSRPVSLDAYTLASITAEYPLTERIAITLRGQNVFDEKPTDIYGYNGPGAGVFVGLKLK
>Mature_633_residues
MNPPRGISLRPMKDAVDMFLRTALLGASALVITASFAQAEDTLTLDPVEVSGLRPIEFDRLTSSVSVLTADDLAIRLSPN
PADQLRAVPGVAISRAGGVGALTQVRMRGAEANHTLVLFEGIEISDPVNGDTDFGLLTALPTGRIEVLRGASSSIHGSDA
IGGVVSISAAREAGLNAFAEAGSFGTVRGALGLSGENFGGAITGFTTDGVDTSGTGGAKDGSEALSGFVNGKVDLGADWS
LSLIGLARGMESEFDSDTDFDGLLDDEDRRTEADQWLVGGALSGVTGPLDHVIRASFNEVKRDNFSDGQKTDVSKGQRTK
LAWLPSLANGAHSLTGILDYEREEYDRTDVQYAGATDASETFESIGVGAEYRYSAGAFDASASARFDDNDGRFDDATTWR
LGAGYAFEQVGGRVRASIGTGVKNPTFTELFGFFPGSFIGNPDLKPEESTSWEIGWEQTVGPVSYSATYFEADLENEIYT
AYNPDFTSSPANRATDSERSGVELAARWQALANLAFFGQATLFDSQDNNGVDEIRVPEATASLGADWLVNPNGFRLGAAL
DYVGEQGDTDFGPFPSRPVSLDAYTLASITAEYPLTERIAITLRGQNVFDEKPTDIYGYNGPGAGVFVGLKLK

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=642, Percent_Identity=26.3239875389408, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1788478, Length=113, Percent_Identity=35.3982300884956, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 66948; Mature: 66948

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPPRGISLRPMKDAVDMFLRTALLGASALVITASFAQAEDTLTLDPVEVSGLRPIEFDR
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCEEECCEECCCCCCCCHHH
LTSSVSVLTADDLAIRLSPNPADQLRAVPGVAISRAGGVGALTQVRMRGAEANHTLVLFE
HHCCEEEEEECCEEEEECCCCHHHHHCCCCCEEECCCCCCHHHHHHHCCCCCCCEEEEEE
GIEISDPVNGDTDFGLLTALPTGRIEVLRGASSSIHGSDAIGGVVSISAAREAGLNAFAE
CCEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHCCHHHHH
AGSFGTVRGALGLSGENFGGAITGFTTDGVDTSGTGGAKDGSEALSGFVNGKVDLGADWS
CCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCC
LSLIGLARGMESEFDSDTDFDGLLDDEDRRTEADQWLVGGALSGVTGPLDHVIRASFNEV
EEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHEEECCHHCCCCCHHHHHHHHHHHHH
KRDNFSDGQKTDVSKGQRTKLAWLPSLANGAHSLTGILDYEREEYDRTDVQYAGATDASE
HCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCHHHCCCCCEEECCCCCHHH
TFESIGVGAEYRYSAGAFDASASARFDDNDGRFDDATTWRLGAGYAFEQVGGRVRASIGT
HHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEHHHHCCEEEEECCC
GVKNPTFTELFGFFPGSFIGNPDLKPEESTSWEIGWEQTVGPVSYSATYFEADLENEIYT
CCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEECCHHHCCCCCEECEEEEEECCCCCEEE
AYNPDFTSSPANRATDSERSGVELAARWQALANLAFFGQATLFDSQDNNGVDEIRVPEAT
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEECCCHH
ASLGADWLVNPNGFRLGAALDYVGEQGDTDFGPFPSRPVSLDAYTLASITAEYPLTERIA
HHCCCCEEECCCCEEECHHHHHHCCCCCCCCCCCCCCCCEECEEEEEHHHCCCCCCEEEE
ITLRGQNVFDEKPTDIYGYNGPGAGVFVGLKLK
EEEECCCCCCCCCCEEECCCCCCCEEEEEEEEC
>Mature Secondary Structure
MNPPRGISLRPMKDAVDMFLRTALLGASALVITASFAQAEDTLTLDPVEVSGLRPIEFDR
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEHHCCCCCEEECCEECCCCCCCCHHH
LTSSVSVLTADDLAIRLSPNPADQLRAVPGVAISRAGGVGALTQVRMRGAEANHTLVLFE
HHCCEEEEEECCEEEEECCCCHHHHHCCCCCEEECCCCCCHHHHHHHCCCCCCCEEEEEE
GIEISDPVNGDTDFGLLTALPTGRIEVLRGASSSIHGSDAIGGVVSISAAREAGLNAFAE
CCEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHCCHHHHH
AGSFGTVRGALGLSGENFGGAITGFTTDGVDTSGTGGAKDGSEALSGFVNGKVDLGADWS
CCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCC
LSLIGLARGMESEFDSDTDFDGLLDDEDRRTEADQWLVGGALSGVTGPLDHVIRASFNEV
EEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHEEECCHHCCCCCHHHHHHHHHHHHH
KRDNFSDGQKTDVSKGQRTKLAWLPSLANGAHSLTGILDYEREEYDRTDVQYAGATDASE
HCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCHHHCCCCCEEECCCCCHHH
TFESIGVGAEYRYSAGAFDASASARFDDNDGRFDDATTWRLGAGYAFEQVGGRVRASIGT
HHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEHHHHCCEEEEECCC
GVKNPTFTELFGFFPGSFIGNPDLKPEESTSWEIGWEQTVGPVSYSATYFEADLENEIYT
CCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEECCHHHCCCCCEECEEEEEECCCCCEEE
AYNPDFTSSPANRATDSERSGVELAARWQALANLAFFGQATLFDSQDNNGVDEIRVPEAT
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEECCCHH
ASLGADWLVNPNGFRLGAALDYVGEQGDTDFGPFPSRPVSLDAYTLASITAEYPLTERIA
HHCCCCEEECCCCEEECHHHHHHCCCCCCCCCCCCCCCCEECEEEEEHHHCCCCCCEEEE
ITLRGQNVFDEKPTDIYGYNGPGAGVFVGLKLK
EEEECCCCCCCCCCEEECCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]