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The map label for this gene is leuD [H]

Identifier: 114800104

GI number: 114800104

Start: 1503517

End: 1504122

Strand: Reverse

Name: leuD [H]

Synonym: HNE_1476

Alternate gene names: 114800104

Gene position: 1504122-1503517 (Counterclockwise)

Preceding gene: 114798881

Following gene: 114797371

Centisome position: 40.6

GC content: 62.21

Gene sequence:

>606_bases
ATGCCGTCTGAACCTTTCGAGCGCGTATCCGGGGTGATCGCGGCGCTTCCGTCAGCCAATATCGACACCGATGTGATCAT
GCCCAAGACGTTTCTGAAGGGCATTGACCGGAGCGGCCTGGAGAAGGGCCTGTTCCATGATCTGCGGTTTGATGAGCAAG
GCGCCCTCCGGCAGGATTTCGTGCTGAACCAGGCGCCCTGGAACACGTCGGCATTTCTTGTCACCGGCGCGAATTTCGGC
TGTGGCTCCTCGCGCGAGCATGCGGTCTGGGGCCTGCGCCAGTTTGGCATCCGGGCGATTATCGCGCGGTCCTTTGCCGG
GATATTCTTCGACAATTGCGCTCGCAACGGCCTGCTTGCCATCGAGGCGGAGGAAGAGACCGTTCGCCAGCTGACCGAAT
GTGGCGAACGCGGCGATAAAATCACGATTGACCTTGCCGCGCAGACCCTCGAGACGGTGCGGGGCGTGGCGGGGTTCGGG
ATCGGCGAGCGGCGTAAATACATGCTTCTGAAGGGGCTCGACGCGGTCGGGATGACGCTACAGTGGCGCACTGAGATCGA
AACCTTCCAATCCCGCTATCTGGGCGCGCGCGCCTGGCTGGCCTGA

Upstream 100 bases:

>100_bases
CAGGGCGCGGGCAGCCGTACGCATCTGATGAGCCCGGCGATGGTGGCGGCGGCGGCGGTTTCCGGGCATCTGTGTGATGT
GCGCACCCTGGAGGTGTCTG

Downstream 100 bases:

>100_bases
CAACACGCAAGGACACTCCCGATGAAAAAGCCTCCCCGGAGGGTGGTCGTCACTCAGAAATTCTTCGATGATGCGGCCAT
CGCCTTTCTGCAGGACAGGA

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MPSEPFERVSGVIAALPSANIDTDVIMPKTFLKGIDRSGLEKGLFHDLRFDEQGALRQDFVLNQAPWNTSAFLVTGANFG
CGSSREHAVWGLRQFGIRAIIARSFAGIFFDNCARNGLLAIEAEEETVRQLTECGERGDKITIDLAAQTLETVRGVAGFG
IGERRKYMLLKGLDAVGMTLQWRTEIETFQSRYLGARAWLA

Sequences:

>Translated_201_residues
MPSEPFERVSGVIAALPSANIDTDVIMPKTFLKGIDRSGLEKGLFHDLRFDEQGALRQDFVLNQAPWNTSAFLVTGANFG
CGSSREHAVWGLRQFGIRAIIARSFAGIFFDNCARNGLLAIEAEEETVRQLTECGERGDKITIDLAAQTLETVRGVAGFG
IGERRKYMLLKGLDAVGMTLQWRTEIETFQSRYLGARAWLA
>Mature_200_residues
PSEPFERVSGVIAALPSANIDTDVIMPKTFLKGIDRSGLEKGLFHDLRFDEQGALRQDFVLNQAPWNTSAFLVTGANFGC
GSSREHAVWGLRQFGIRAIIARSFAGIFFDNCARNGLLAIEAEEETVRQLTECGERGDKITIDLAAQTLETVRGVAGFGI
GERRKYMLLKGLDAVGMTLQWRTEIETFQSRYLGARAWLA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=194, Percent_Identity=37.6288659793814, Blast_Score=137, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6321429, Length=202, Percent_Identity=46.039603960396, Blast_Score=167, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22232; Mature: 22100

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSEPFERVSGVIAALPSANIDTDVIMPKTFLKGIDRSGLEKGLFHDLRFDEQGALRQDF
CCCCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHH
VLNQAPWNTSAFLVTGANFGCGSSREHAVWGLRQFGIRAIIARSFAGIFFDNCARNGLLA
CCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IEAEEETVRQLTECGERGDKITIDLAAQTLETVRGVAGFGIGERRKYMLLKGLDAVGMTL
EECCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHCCEE
QWRTEIETFQSRYLGARAWLA
EEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PSEPFERVSGVIAALPSANIDTDVIMPKTFLKGIDRSGLEKGLFHDLRFDEQGALRQDF
CCCHHHHHHHHHHHCCCCCCCCCEECHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHH
VLNQAPWNTSAFLVTGANFGCGSSREHAVWGLRQFGIRAIIARSFAGIFFDNCARNGLLA
CCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IEAEEETVRQLTECGERGDKITIDLAAQTLETVRGVAGFGIGERRKYMLLKGLDAVGMTL
EECCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHCCEE
QWRTEIETFQSRYLGARAWLA
EEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA