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The map label for this gene is leuC2 [H]

Identifier: 114798881

GI number: 114798881

Start: 1504112

End: 1505542

Strand: Reverse

Name: leuC2 [H]

Synonym: HNE_1477

Alternate gene names: 114798881

Gene position: 1505542-1504112 (Counterclockwise)

Preceding gene: 114797316

Following gene: 114800104

Centisome position: 40.64

GC content: 63.73

Gene sequence:

>1431_bases
ATGGCAGCATACAGGACATTGTACGACAAACTGGTCGACAGCCACACGGTGCGGGCGCTCGATGCAGGTGGCGGCGAAGT
GCTGCTTTATGTCGATCGCACCGTGCTCAACGAATATACCAGCCCGCAGGCATTTACCGGACTGCGGGACGCCGGGCGCG
CCGTGTTCCGGCCGCAATCTGCGCTGGGCGTCATCGACCATGTGAACTCCACGCGGCCCGAACGGATCGCCTTACCGCGC
GTGCAGGCCGATGATCTGCAGATGTCTTATTTCGTCAGCAACTGCAAAGAATTCGGCATCGAACTGTTCGATCTCTATCA
TCGCCAGCAGGGCATTGAACATGTGGTGATGCCCGAGCTGGGCCTGGCTCTGCCCGGTCTCGTGATCGCAGCGGGCGACA
GCCATACGACCACCTATGGCGCTTTCGGAGCGCTCGGATTTGGCATTGGCACATCCGACATCGAACACTTCCTGGCGACG
CAGACGCTGGTTTACCGGCGGCTGCGCAATATGGAGATTGCGGTGGCCGGTCACCTGCCTCCGGGCGTGACGGCGAAGGA
TCTCATCATGGCGGTTATCCGCAGGATCGGCGCAGATGGCGCGCAGGGTCATGCGGTGGAGTTTACGGGCCCGGCGATCC
GCACCCTGTCGATCGAAGGGCGAATGACGATCTGCAATATGGCAGTTGAATGTGGCGCGCGCGGCGCGCTGATTGCCCCG
GACGACAAGACCTTTGCCTATCTGGAAGGCAAACCGCGCGCCCCCAAGGGACATGCGCTGATAGCGGCCCTGGAAGCCTG
GCGGATGCTGCGCACCGATGACGGCGCTGTGTTTGACGCCCGCATTGAAATCTCTGCCGGTGACATTGAACCGATGGTGA
CCTGGGGCACGAGCCCGGATCAGGCCGCGCCGATTTCAGAGGCCGTTCCTGACCCCGCCCTTGAAACCAGCCCTGCCCGG
CGCCGGGATGCTGAGCGCGCGCTGGGCTATATGGGATTGTCGCCCGGAACGCGCCTGACGGACATCCGGATTGATCATGC
CTTCATCGGCTCCTGTACCAATGCCCGGCTGGAAGATCTGCGCGGCGCCGCCCGCATCGTGAAAGGGCGCAGGGCCGCTG
CAGGGGTGAGCGCCATTGTCGTGCCCGGATCCCGCGAGGTCCGGCGCCTGGCTGAGGCCGAAGGGCTCGACCGGATTTTC
CGCGAGGCCGGCTTTGAATGGCGCGAGCCGGGATGCTCGATGTGCCTTGCCATGAATGACGATATCATTCCGCCGGGCGA
ACGCTGTGCCTCCAGCACCAACCGGAACTTTGAAGGCCGCCAGGGCGCGGGCAGCCGTACGCATCTGATGAGCCCGGCGA
TGGTGGCGGCGGCGGCGGTTTCCGGGCATCTGTGTGATGTGCGCACCCTGGAGGTGTCTGATGCCGTCTGA

Upstream 100 bases:

>100_bases
GCCTTTCATTCTCGAATGGATTGATACAATATTTGCACAACACCGGGCGATGGCCCCCTGCTAAACCCTCCGGAAGACCA
CACGGATTATTCGGGGCGTC

Downstream 100 bases:

>100_bases
ACCTTTCGAGCGCGTATCCGGGGTGATCGCGGCGCTTCCGTCAGCCAATATCGACACCGATGTGATCATGCCCAAGACGT
TTCTGAAGGGCATTGACCGG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAAYRTLYDKLVDSHTVRALDAGGGEVLLYVDRTVLNEYTSPQAFTGLRDAGRAVFRPQSALGVIDHVNSTRPERIALPR
VQADDLQMSYFVSNCKEFGIELFDLYHRQQGIEHVVMPELGLALPGLVIAAGDSHTTTYGAFGALGFGIGTSDIEHFLAT
QTLVYRRLRNMEIAVAGHLPPGVTAKDLIMAVIRRIGADGAQGHAVEFTGPAIRTLSIEGRMTICNMAVECGARGALIAP
DDKTFAYLEGKPRAPKGHALIAALEAWRMLRTDDGAVFDARIEISAGDIEPMVTWGTSPDQAAPISEAVPDPALETSPAR
RRDAERALGYMGLSPGTRLTDIRIDHAFIGSCTNARLEDLRGAARIVKGRRAAAGVSAIVVPGSREVRRLAEAEGLDRIF
REAGFEWREPGCSMCLAMNDDIIPPGERCASSTNRNFEGRQGAGSRTHLMSPAMVAAAAVSGHLCDVRTLEVSDAV

Sequences:

>Translated_476_residues
MAAYRTLYDKLVDSHTVRALDAGGGEVLLYVDRTVLNEYTSPQAFTGLRDAGRAVFRPQSALGVIDHVNSTRPERIALPR
VQADDLQMSYFVSNCKEFGIELFDLYHRQQGIEHVVMPELGLALPGLVIAAGDSHTTTYGAFGALGFGIGTSDIEHFLAT
QTLVYRRLRNMEIAVAGHLPPGVTAKDLIMAVIRRIGADGAQGHAVEFTGPAIRTLSIEGRMTICNMAVECGARGALIAP
DDKTFAYLEGKPRAPKGHALIAALEAWRMLRTDDGAVFDARIEISAGDIEPMVTWGTSPDQAAPISEAVPDPALETSPAR
RRDAERALGYMGLSPGTRLTDIRIDHAFIGSCTNARLEDLRGAARIVKGRRAAAGVSAIVVPGSREVRRLAEAEGLDRIF
REAGFEWREPGCSMCLAMNDDIIPPGERCASSTNRNFEGRQGAGSRTHLMSPAMVAAAAVSGHLCDVRTLEVSDAV
>Mature_475_residues
AAYRTLYDKLVDSHTVRALDAGGGEVLLYVDRTVLNEYTSPQAFTGLRDAGRAVFRPQSALGVIDHVNSTRPERIALPRV
QADDLQMSYFVSNCKEFGIELFDLYHRQQGIEHVVMPELGLALPGLVIAAGDSHTTTYGAFGALGFGIGTSDIEHFLATQ
TLVYRRLRNMEIAVAGHLPPGVTAKDLIMAVIRRIGADGAQGHAVEFTGPAIRTLSIEGRMTICNMAVECGARGALIAPD
DKTFAYLEGKPRAPKGHALIAALEAWRMLRTDDGAVFDARIEISAGDIEPMVTWGTSPDQAAPISEAVPDPALETSPARR
RDAERALGYMGLSPGTRLTDIRIDHAFIGSCTNARLEDLRGAARIVKGRRAAAGVSAIVVPGSREVRRLAEAEGLDRIFR
EAGFEWREPGCSMCLAMNDDIIPPGERCASSTNRNFEGRQGAGSRTHLMSPAMVAAAAVSGHLCDVRTLEVSDAV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=365, Percent_Identity=29.8630136986301, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI41352693, Length=385, Percent_Identity=26.2337662337662, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI8659555, Length=388, Percent_Identity=25.2577319587629, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786259, Length=467, Percent_Identity=56.3169164882227, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI1787531, Length=321, Percent_Identity=27.1028037383178, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87081781, Length=377, Percent_Identity=23.6074270557029, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=362, Percent_Identity=31.4917127071823, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=368, Percent_Identity=30.9782608695652, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=300, Percent_Identity=31.6666666666667, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=374, Percent_Identity=25.9358288770053, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6321429, Length=476, Percent_Identity=52.7310924369748, Blast_Score=497, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320440, Length=460, Percent_Identity=26.0869565217391, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=401, Percent_Identity=28.6783042394015, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=476, Percent_Identity=27.5210084033613, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281365315, Length=477, Percent_Identity=27.4633123689727, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17864292, Length=477, Percent_Identity=27.4633123689727, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161076999, Length=383, Percent_Identity=28.7206266318538, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI28571643, Length=477, Percent_Identity=26.6247379454927, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24645686, Length=367, Percent_Identity=27.2479564032698, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137564, Length=367, Percent_Identity=26.158038147139, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51190; Mature: 51059

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAYRTLYDKLVDSHTVRALDAGGGEVLLYVDRTVLNEYTSPQAFTGLRDAGRAVFRPQS
CCHHHHHHHHHHCCCCEEEEECCCCEEEEEECHHHHHCCCCCHHHHHHHHCCHHHCCCCH
ALGVIDHVNSTRPERIALPRVQADDLQMSYFVSNCKEFGIELFDLYHRQQGIEHVVMPEL
HHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEECCHH
GLALPGLVIAAGDSHTTTYGAFGALGFGIGTSDIEHFLATQTLVYRRLRNMEIAVAGHLP
CCCCCCEEEECCCCCCCCHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
PGVTAKDLIMAVIRRIGADGAQGHAVEFTGPAIRTLSIEGRMTICNMAVECGARGALIAP
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCEEEEHHHHHCCCCCCEECC
DDKTFAYLEGKPRAPKGHALIAALEAWRMLRTDDGAVFDARIEISAGDIEPMVTWGTSPD
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCCCC
QAAPISEAVPDPALETSPARRRDAERALGYMGLSPGTRLTDIRIDHAFIGSCTNARLEDL
CCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHCCCCCHHHHHH
RGAARIVKGRRAAAGVSAIVVPGSREVRRLAEAEGLDRIFREAGFEWREPGCSMCLAMND
HHHHHHHHCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECC
DIIPPGERCASSTNRNFEGRQGAGSRTHLMSPAMVAAAAVSGHLCDVRTLEVSDAV
CCCCCCHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCEEEEEECCCCC
>Mature Secondary Structure 
AAYRTLYDKLVDSHTVRALDAGGGEVLLYVDRTVLNEYTSPQAFTGLRDAGRAVFRPQS
CHHHHHHHHHHCCCCEEEEECCCCEEEEEECHHHHHCCCCCHHHHHHHHCCHHHCCCCH
ALGVIDHVNSTRPERIALPRVQADDLQMSYFVSNCKEFGIELFDLYHRQQGIEHVVMPEL
HHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEECCHH
GLALPGLVIAAGDSHTTTYGAFGALGFGIGTSDIEHFLATQTLVYRRLRNMEIAVAGHLP
CCCCCCEEEECCCCCCCCHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
PGVTAKDLIMAVIRRIGADGAQGHAVEFTGPAIRTLSIEGRMTICNMAVECGARGALIAP
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEECCCEEEEHHHHHCCCCCCEECC
DDKTFAYLEGKPRAPKGHALIAALEAWRMLRTDDGAVFDARIEISAGDIEPMVTWGTSPD
CCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCCCC
QAAPISEAVPDPALETSPARRRDAERALGYMGLSPGTRLTDIRIDHAFIGSCTNARLEDL
CCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECHHHHCCCCCHHHHHH
RGAARIVKGRRAAAGVSAIVVPGSREVRRLAEAEGLDRIFREAGFEWREPGCSMCLAMND
HHHHHHHHCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECC
DIIPPGERCASSTNRNFEGRQGAGSRTHLMSPAMVAAAAVSGHLCDVRTLEVSDAV
CCCCCCHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA