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The map label for this gene is leuB [H]

Identifier: 114800090

GI number: 114800090

Start: 46142

End: 47194

Strand: Direct

Name: leuB [H]

Synonym: HNE_0056

Alternate gene names: 114800090

Gene position: 46142-47194 (Clockwise)

Preceding gene: 114797061

Following gene: 114799080

Centisome position: 1.25

GC content: 64.86

Gene sequence:

>1053_bases
ATGAAACAGACTCTCCTCCTATTGCCGGGCGATGGCATCGGCCCCGAAGTTGTCGCACAGGCCCGGCGTATCGCCGAAGC
GCTTGTGCCGGACGTGCAGATTGAAGAAGGGCTCGTCGGTGGCGCAAGCTTTGATGCCCACGGCACGCCCCTGACCGATG
AGACGCTGGACCGTGCCCGGAAGGCCAGCGCCGTTCTGCTTGGCGCCGTTGGCGGCCCCAGATGGACGGCAACGGCGCGT
GATAAGCGCCCTGAAGCGGGTCTCCTGGCGCTCCGCAAGGGACTGGATGTCTTTGCCAATCTGCGACCGGCTTTCTGTTT
TCCAGCGCTGGTCGATGCCTCCAGCCTGAAGAAGGACCGCGTCGAAGGGCTTGATCTGATGATCGTCCGCGAGCTGACCG
GCGGGGTCTATTTCGGCCAGCCTCGCGGCATCGACGAAGAGGGCGGCCTGCGCCGCGGCTATGACACGGCGATTTATACC
CACCCGGAAGTCGAGCGCGTTGCCCGCATCGCGTTTGACATAGCGCGTGGCCGGAATGGCCGCGTTACCTCGGTTGAAAA
ATCCAATGTGATGGAATCTGGCCTCTTCTGGCGCCAGGAAGTTACCCTCGCGCATGCCGAACTCGGCGCCGGGGTGGAGC
TGGCCCACATGTATGCAGATGCCTGCGCCATGGAACTCGTCCGCGCGCCCAAGCAGTTTGACGTGATCCTGGCAGATAAT
CTCTTCGGCGACATCCTGTCGGACGCGGCGGCTATGTTGACCGGCTCGATCGGTATGTTGCCATCCGCCTCGCTAGGCGC
GCCCGGCACGCCGGGCCTCTATGAGCCGGTCCACGGCTCCGCGCCCGACATTGCCGGGCAGGGCATCGCCAACCCCTGCG
CGGCCATCCTGTCGCTCGAAATGGCGCTGCGTTGGTCGCTCGGTAAAGAGAAAGCCGCTGATACGCTGCTCGCCGCCGTT
GGCAAGGCGCTCGACCGCGGTGCCCGCACGCGGGACCTTGGCGGAGATCTGAGCACCCGTGCAATGGCGGACGCGGTTCT
CAACGCGCTCTGA

Upstream 100 bases:

>100_bases
CCTGCTGATCGAAGGGATATCGCAACAAAGCGAACCTGAAGAGGCAATGGCACTTTAAGCCGCCTCGACGGCCCGTAATT
TTCCTGCCAAACAGCCGCGC

Downstream 100 bases:

>100_bases
GCGCCGTAACGCGTCCGGATTTGGACAAGCCGGCCACCTTGTGACATCCTCCGCTCAAAAGAAGAGCGGAGGAAACAAGC
AATGTCGTATGCTGCCGGGC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKQTLLLLPGDGIGPEVVAQARRIAEALVPDVQIEEGLVGGASFDAHGTPLTDETLDRARKASAVLLGAVGGPRWTATAR
DKRPEAGLLALRKGLDVFANLRPAFCFPALVDASSLKKDRVEGLDLMIVRELTGGVYFGQPRGIDEEGGLRRGYDTAIYT
HPEVERVARIAFDIARGRNGRVTSVEKSNVMESGLFWRQEVTLAHAELGAGVELAHMYADACAMELVRAPKQFDVILADN
LFGDILSDAAAMLTGSIGMLPSASLGAPGTPGLYEPVHGSAPDIAGQGIANPCAAILSLEMALRWSLGKEKAADTLLAAV
GKALDRGARTRDLGGDLSTRAMADAVLNAL

Sequences:

>Translated_350_residues
MKQTLLLLPGDGIGPEVVAQARRIAEALVPDVQIEEGLVGGASFDAHGTPLTDETLDRARKASAVLLGAVGGPRWTATAR
DKRPEAGLLALRKGLDVFANLRPAFCFPALVDASSLKKDRVEGLDLMIVRELTGGVYFGQPRGIDEEGGLRRGYDTAIYT
HPEVERVARIAFDIARGRNGRVTSVEKSNVMESGLFWRQEVTLAHAELGAGVELAHMYADACAMELVRAPKQFDVILADN
LFGDILSDAAAMLTGSIGMLPSASLGAPGTPGLYEPVHGSAPDIAGQGIANPCAAILSLEMALRWSLGKEKAADTLLAAV
GKALDRGARTRDLGGDLSTRAMADAVLNAL
>Mature_350_residues
MKQTLLLLPGDGIGPEVVAQARRIAEALVPDVQIEEGLVGGASFDAHGTPLTDETLDRARKASAVLLGAVGGPRWTATAR
DKRPEAGLLALRKGLDVFANLRPAFCFPALVDASSLKKDRVEGLDLMIVRELTGGVYFGQPRGIDEEGGLRRGYDTAIYT
HPEVERVARIAFDIARGRNGRVTSVEKSNVMESGLFWRQEVTLAHAELGAGVELAHMYADACAMELVRAPKQFDVILADN
LFGDILSDAAAMLTGSIGMLPSASLGAPGTPGLYEPVHGSAPDIAGQGIANPCAAILSLEMALRWSLGKEKAADTLLAAV
GKALDRGARTRDLGGDLSTRAMADAVLNAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=339, Percent_Identity=34.5132743362832, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI28178821, Length=357, Percent_Identity=30.5322128851541, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI28178816, Length=353, Percent_Identity=30.3116147308782, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4758582, Length=359, Percent_Identity=27.8551532033426, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI28178838, Length=336, Percent_Identity=27.9761904761905, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI28178819, Length=200, Percent_Identity=36, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI87081683, Length=349, Percent_Identity=48.9971346704871, Blast_Score=313, Evalue=8e-87,
Organism=Escherichia coli, GI1788101, Length=367, Percent_Identity=33.2425068119891, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1787381, Length=379, Percent_Identity=28.7598944591029, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=34.2105263157895, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=355, Percent_Identity=30.9859154929577, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=358, Percent_Identity=31.0055865921788, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=356, Percent_Identity=28.9325842696629, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=41.8994413407821, Blast_Score=249, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6322097, Length=363, Percent_Identity=36.0881542699724, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6324291, Length=356, Percent_Identity=32.0224719101124, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6324709, Length=361, Percent_Identity=34.0720221606648, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24643268, Length=354, Percent_Identity=33.3333333333333, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24643270, Length=354, Percent_Identity=32.7683615819209, Blast_Score=135, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24661184, Length=359, Percent_Identity=31.1977715877437, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI281362242, Length=357, Percent_Identity=27.4509803921569, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648872, Length=357, Percent_Identity=27.4509803921569, Blast_Score=107, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161078635, Length=360, Percent_Identity=27.5, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI161078637, Length=360, Percent_Identity=27.5, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI161078633, Length=360, Percent_Identity=27.5, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24650122, Length=360, Percent_Identity=27.5, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI161078639, Length=355, Percent_Identity=27.887323943662, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI20130355, Length=190, Percent_Identity=28.4210526315789, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36858; Mature: 36858

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTLLLLPGDGIGPEVVAQARRIAEALVPDVQIEEGLVGGASFDAHGTPLTDETLDRAR
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHH
KASAVLLGAVGGPRWTATARDKRPEAGLLALRKGLDVFANLRPAFCFPALVDASSLKKDR
HHCEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHH
VEGLDLMIVRELTGGVYFGQPRGIDEEGGLRRGYDTAIYTHPEVERVARIAFDIARGRNG
HCCCCEEEEEHHHCCEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCC
RVTSVEKSNVMESGLFWRQEVTLAHAELGAGVELAHMYADACAMELVRAPKQFDVILADN
CEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHEEEEHHH
LFGDILSDAAAMLTGSIGMLPSASLGAPGTPGLYEPVHGSAPDIAGQGIANPCAAILSLE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MALRWSLGKEKAADTLLAAVGKALDRGARTRDLGGDLSTRAMADAVLNAL
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQTLLLLPGDGIGPEVVAQARRIAEALVPDVQIEEGLVGGASFDAHGTPLTDETLDRAR
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHH
KASAVLLGAVGGPRWTATARDKRPEAGLLALRKGLDVFANLRPAFCFPALVDASSLKKDR
HHCEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHH
VEGLDLMIVRELTGGVYFGQPRGIDEEGGLRRGYDTAIYTHPEVERVARIAFDIARGRNG
HCCCCEEEEEHHHCCEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCCC
RVTSVEKSNVMESGLFWRQEVTLAHAELGAGVELAHMYADACAMELVRAPKQFDVILADN
CEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHEEEEHHH
LFGDILSDAAAMLTGSIGMLPSASLGAPGTPGLYEPVHGSAPDIAGQGIANPCAAILSLE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MALRWSLGKEKAADTLLAAVGKALDRGARTRDLGGDLSTRAMADAVLNAL
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]