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The map label for this gene is rne [H]

Identifier: 114800079

GI number: 114800079

Start: 679299

End: 682028

Strand: Reverse

Name: rne [H]

Synonym: HNE_0673

Alternate gene names: 114800079

Gene position: 682028-679299 (Counterclockwise)

Preceding gene: 114798232

Following gene: 114797087

Centisome position: 18.41

GC content: 63.96

Gene sequence:

>2730_bases
ATGAGCAGATTGATGCTGATCGACGCTGCCCACCCCGAAGAAACGCGGGTGGCCATCGTCAACAACGGCCAGGTTGACGA
TTTCGACTTTGAGGCAACCGGCAACGAACAACTCCGCGGAAACATCTATCTCGCCAAAGTGACCCGTGTGGAGCCGTCGC
TCCAGGCGGCTTTTGTCGAGTATGGTGGCAACCGCCACGGTTTTCTCGCCTTCAGCGAGATTCACCCCGATTATTACCAG
CTGCCCGCCGCTGACCGCGAATTGCTGCTGAAGGAAGCGGCTGAAGAGGCCGCCGCCGCCATCGAAGACGATGATGATGG
CCCTTACGTGCCCCATGGCATGAGCTCCGAGATGGGTAACGGCGCCTCTGATGAGGGCGACGAGGACGACGATCACGGCG
ACGATGGCGATGAAGACACCGCCTCTGCCGACGATGACGGCGAGGACGAAGGCGATGACGCCGCCCCGCGCGCCGCCAAA
CCGGCCCGCCGCAATGCCAAGAAGCAGACCCAGCGCACGCCGATTTCCAAGCGCTACAAGATCCAGGAAGTCATCCGCCG
CCGTCAGGTGATGCTGGTGCAGGTGGTCAAGGAAGAACGCGGCAACAAAGGCGCCGCGCTCACCACCTATCTCTCGCTGG
CAGGGCGCTACTCCGTTCTGATGCCGAACACCCCGCGCGGGGGCGGCATTTCCCGCAAGATCGTCAATTCGGCCGACCGC
AAGCGCCTGAAATCCATCGTTTCGGAACTGGATGTGCCCGAAGGCCAGGGCCTGATCGTGCGCACGGCCGGCGCCAAGCG
CACCAAGATCGAAATCAAGCGCGACTATGATTACCTCTCGAAACTGTGGGAACAGATTGTCGAGAAGACCCTTTCCTCCG
AAGCCCCGGCGCTGATCAATGCCGAAGGCGGCTTGGTGCACCGGGCCATGCGGGACATGTTCGACAAGGAAATCGAGGAA
GTCTGGGTTCAGGGCGATGAGGCTTACCGCGAGGCCAAAGACCTTGCGAAGATCATCATGCCGAGCCAGGCCAAGAAGGT
TCAGCAATGGCGCGAGGAAGACCCGCTCTATGTAGCTGAAGCAGTCGAGAACCAGCTTGATTCCATTTACTCTCCCGTCG
TTCAGCTGAAATCAGGCGGCTATCTGGTGATCAACCAGACCGAAGCCCTGGTCGCCATCGACGTGAACTCGGGCAAATCG
ACCCGCGAGCGCAATATCGAGCAGACGGCTGTACGCACCAACCTGGAAGCAGCTGAAGAAGCCTGCCGCCAGATGCGCCT
GCGCGACCTTGCCGGCCTCATCGTGATCGATTTCATCGACATGGAGGAGAACAAGAACAATCGCGCGGTCGAAAAGCGCC
TGAAGGAATGCCTGAAGGTGGACCGGGCGCGCGTGCAGCATGGCCGCATCTCGCAGTTCGGCCTGATGGAAATTTCCCGC
CAGCGCCGCCGCCAGGGCGTGTTGCAGGCAACCTCCGACCCCTGCTCTGCCTGTAACGGCACGGGCCGCCGCCGCTCGAT
CCCGTCCGCTGCGCTGCAGCTGATCCGGGCCATCGAAGCGCGCGCAGCAACCGGCGGCCTCAAAGGCATTGGCGTGAAAG
CGCCAACCGATGTGGCGCTCTATATCCTCAACAACAAGCGCGAGGCGCTGATTGAAATCGAGCGGCTCGCGGGCTTTGCG
ATCTCCATCCACTCCTCCGAAGACATGCTGCCGGGCGATTTCCAGATCGACGCCGACCGCGACCCGAACGCCAAGCCGAA
GAAACGCTCCACGCCGCGCTCGCTGATGAAGCCGACGCCGCCGTCTGCACGCGACGACGACGATGAAGAGATCGACGACG
AAGACGTGATCGAGGACGAGGCCGGCGAAGACGATGACGGCGAAACGCCCGCTGCGGCTGCAAACAAAGACAAGCAAGGT
GGCGGACGCCGCCGGCGCCGTCGCGGGGGCCGCAATCGCGGTGAAGCCCGCGAGACGGGCCTTGAAGTGGTCGACGCCGA
ACCAGCCGCTGATGCTGACGCAAGCGAAGACGATGACGACGAGGACGACGGCGAGGACACCTCCAATGGCAGCGCGGAAG
CGGGCTCCGATGACAGCGACGCCGCGCGCAAGCGCCGCCGCCGGGGACGCCGGGGTGGACGCCGCCGGAGACGTGGCGGT
GAAGGCCGCGGCGAGAATGGTGACATTGCCGCCATTGCCGATGGTGGGGCTTTCCTGGATGGCTTGTCTGTCAGCGCCGA
TGTGATGCTGTCCGAAGACGAGCCTGCGATCGGCCTGCCGGCAGCTGCCGATACGCAGGACACGGACGACGAGTCGGAGA
CGAAAGCGGCCCCTGCCGAGGCAGCAGCTGACGCCGCCGAGACCGATGAGGCGCCAGCTGCCACTGAAGAGGCCGCGCCA
CGCCCCGCCAGATCTCGCTCACGCCGGGGACGCGGCAGCAAACCGGCTCCGGAAGAAACACCGTCAGCTGAAGAAACCGC
GCCTGCGGCAGAAGCTGAAGCCGAAGCCGAAGCCGAAGCGCCGGTTGTGGAGGCTGCTCAAGAAGCTGCGCCAGAGCCGG
TCGCCGAAGCCGAGCCGGAACCTGAAATGGCCCAGGCTGATTTTGCGCCGCCCAAAGCCGCCGCTGTTGAGGCTGAACCT
GCGCCGGAACCAGAGGGTGCGCCCGCTCCCTCTGCGGCAGAGCCCCCTGCTCCGAAGCGAGCGGGCTGGTGGAACCGCGG
GAAGAAGTGA

Upstream 100 bases:

>100_bases
TCAGAAGGCGCCTTCCCCTTATCCCCCTATTCCGGCCTGCCAGCCAACCAGAGGCGCGCTGCGCTCTGGCGAGCGGCGCC
GGACCGAAAGGTTCTGTTTA

Downstream 100 bases:

>100_bases
CCCTTCGCGATTTTTTCCGATAACGGATGAAATAAAAAAGGCCGGAGCGATGCTCCGGCCTTTTCTTTTTAGAAGTTATT
CGAAGCTTCGCGGTGGCCTA

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 909; Mature: 908

Protein sequence:

>909_residues
MSRLMLIDAAHPEETRVAIVNNGQVDDFDFEATGNEQLRGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQ
LPAADRELLLKEAAEEAAAAIEDDDDGPYVPHGMSSEMGNGASDEGDEDDDHGDDGDEDTASADDDGEDEGDDAAPRAAK
PARRNAKKQTQRTPISKRYKIQEVIRRRQVMLVQVVKEERGNKGAALTTYLSLAGRYSVLMPNTPRGGGISRKIVNSADR
KRLKSIVSELDVPEGQGLIVRTAGAKRTKIEIKRDYDYLSKLWEQIVEKTLSSEAPALINAEGGLVHRAMRDMFDKEIEE
VWVQGDEAYREAKDLAKIIMPSQAKKVQQWREEDPLYVAEAVENQLDSIYSPVVQLKSGGYLVINQTEALVAIDVNSGKS
TRERNIEQTAVRTNLEAAEEACRQMRLRDLAGLIVIDFIDMEENKNNRAVEKRLKECLKVDRARVQHGRISQFGLMEISR
QRRRQGVLQATSDPCSACNGTGRRRSIPSAALQLIRAIEARAATGGLKGIGVKAPTDVALYILNNKREALIEIERLAGFA
ISIHSSEDMLPGDFQIDADRDPNAKPKKRSTPRSLMKPTPPSARDDDDEEIDDEDVIEDEAGEDDDGETPAAAANKDKQG
GGRRRRRRGGRNRGEARETGLEVVDAEPAADADASEDDDDEDDGEDTSNGSAEAGSDDSDAARKRRRRGRRGGRRRRRGG
EGRGENGDIAAIADGGAFLDGLSVSADVMLSEDEPAIGLPAAADTQDTDDESETKAAPAEAAADAAETDEAPAATEEAAP
RPARSRSRRGRGSKPAPEETPSAEETAPAAEAEAEAEAEAPVVEAAQEAAPEPVAEAEPEPEMAQADFAPPKAAAVEAEP
APEPEGAPAPSAAEPPAPKRAGWWNRGKK

Sequences:

>Translated_909_residues
MSRLMLIDAAHPEETRVAIVNNGQVDDFDFEATGNEQLRGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQ
LPAADRELLLKEAAEEAAAAIEDDDDGPYVPHGMSSEMGNGASDEGDEDDDHGDDGDEDTASADDDGEDEGDDAAPRAAK
PARRNAKKQTQRTPISKRYKIQEVIRRRQVMLVQVVKEERGNKGAALTTYLSLAGRYSVLMPNTPRGGGISRKIVNSADR
KRLKSIVSELDVPEGQGLIVRTAGAKRTKIEIKRDYDYLSKLWEQIVEKTLSSEAPALINAEGGLVHRAMRDMFDKEIEE
VWVQGDEAYREAKDLAKIIMPSQAKKVQQWREEDPLYVAEAVENQLDSIYSPVVQLKSGGYLVINQTEALVAIDVNSGKS
TRERNIEQTAVRTNLEAAEEACRQMRLRDLAGLIVIDFIDMEENKNNRAVEKRLKECLKVDRARVQHGRISQFGLMEISR
QRRRQGVLQATSDPCSACNGTGRRRSIPSAALQLIRAIEARAATGGLKGIGVKAPTDVALYILNNKREALIEIERLAGFA
ISIHSSEDMLPGDFQIDADRDPNAKPKKRSTPRSLMKPTPPSARDDDDEEIDDEDVIEDEAGEDDDGETPAAAANKDKQG
GGRRRRRRGGRNRGEARETGLEVVDAEPAADADASEDDDDEDDGEDTSNGSAEAGSDDSDAARKRRRRGRRGGRRRRRGG
EGRGENGDIAAIADGGAFLDGLSVSADVMLSEDEPAIGLPAAADTQDTDDESETKAAPAEAAADAAETDEAPAATEEAAP
RPARSRSRRGRGSKPAPEETPSAEETAPAAEAEAEAEAEAPVVEAAQEAAPEPVAEAEPEPEMAQADFAPPKAAAVEAEP
APEPEGAPAPSAAEPPAPKRAGWWNRGKK
>Mature_908_residues
SRLMLIDAAHPEETRVAIVNNGQVDDFDFEATGNEQLRGNIYLAKVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQL
PAADRELLLKEAAEEAAAAIEDDDDGPYVPHGMSSEMGNGASDEGDEDDDHGDDGDEDTASADDDGEDEGDDAAPRAAKP
ARRNAKKQTQRTPISKRYKIQEVIRRRQVMLVQVVKEERGNKGAALTTYLSLAGRYSVLMPNTPRGGGISRKIVNSADRK
RLKSIVSELDVPEGQGLIVRTAGAKRTKIEIKRDYDYLSKLWEQIVEKTLSSEAPALINAEGGLVHRAMRDMFDKEIEEV
WVQGDEAYREAKDLAKIIMPSQAKKVQQWREEDPLYVAEAVENQLDSIYSPVVQLKSGGYLVINQTEALVAIDVNSGKST
RERNIEQTAVRTNLEAAEEACRQMRLRDLAGLIVIDFIDMEENKNNRAVEKRLKECLKVDRARVQHGRISQFGLMEISRQ
RRRQGVLQATSDPCSACNGTGRRRSIPSAALQLIRAIEARAATGGLKGIGVKAPTDVALYILNNKREALIEIERLAGFAI
SIHSSEDMLPGDFQIDADRDPNAKPKKRSTPRSLMKPTPPSARDDDDEEIDDEDVIEDEAGEDDDGETPAAAANKDKQGG
GRRRRRRGGRNRGEARETGLEVVDAEPAADADASEDDDDEDDGEDTSNGSAEAGSDDSDAARKRRRRGRRGGRRRRRGGE
GRGENGDIAAIADGGAFLDGLSVSADVMLSEDEPAIGLPAAADTQDTDDESETKAAPAEAAADAAETDEAPAATEEAAPR
PARSRSRRGRGSKPAPEETPSAEETAPAAEAEAEAEAEAPVVEAAQEAAPEPVAEAEPEPEMAQADFAPPKAAAVEAEPA
PEPEGAPAPSAAEPPAPKRAGWWNRGKK

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. It also cleaves RNA I, a molecule that controls the replication of colE1 plasmid DNA. It is the major endoribonuclease participating in mRNA turnover in E.coli. It initiates the decay of RNAs by

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=378, Percent_Identity=45.2380952380952, Blast_Score=318, Evalue=1e-87,
Organism=Escherichia coli, GI87082235, Length=338, Percent_Identity=33.7278106508876, Blast_Score=190, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 98543; Mature: 98412

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLMLIDAAHPEETRVAIVNNGQVDDFDFEATGNEQLRGNIYLAKVTRVEPSLQAAFVE
CCCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCEEEEEEEECCHHHHHHHHH
YGGNRHGFLAFSEIHPDYYQLPAADRELLLKEAAEEAAAAIEDDDDGPYVPHGMSSEMGN
HCCCCCCEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GASDEGDEDDDHGDDGDEDTASADDDGEDEGDDAAPRAAKPARRNAKKQTQRTPISKRYK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHH
IQEVIRRRQVMLVQVVKEERGNKGAALTTYLSLAGRYSVLMPNTPRGGGISRKIVNSADR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
KRLKSIVSELDVPEGQGLIVRTAGAKRTKIEIKRDYDYLSKLWEQIVEKTLSSEAPALIN
HHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEE
AEGGLVHRAMRDMFDKEIEEVWVQGDEAYREAKDLAKIIMPSQAKKVQQWREEDPLYVAE
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHH
AVENQLDSIYSPVVQLKSGGYLVINQTEALVAIDVNSGKSTRERNIEQTAVRTNLEAAEE
HHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
ACRQMRLRDLAGLIVIDFIDMEENKNNRAVEKRLKECLKVDRARVQHGRISQFGLMEISR
HHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QRRRQGVLQATSDPCSACNGTGRRRSIPSAALQLIRAIEARAATGGLKGIGVKAPTDVAL
HHHHCCCHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
YILNNKREALIEIERLAGFAISIHSSEDMLPGDFQIDADRDPNAKPKKRSTPRSLMKPTP
EEECCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCHHHCCCCC
PSARDDDDEEIDDEDVIEDEAGEDDDGETPAAAANKDKQGGGRRRRRRGGRNRGEARETG
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHC
LEVVDAEPAADADASEDDDDEDDGEDTSNGSAEAGSDDSDAARKRRRRGRRGGRRRRRGG
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCC
EGRGENGDIAAIADGGAFLDGLSVSADVMLSEDEPAIGLPAAADTQDTDDESETKAAPAE
CCCCCCCCEEEEECCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCHH
AAADAAETDEAPAATEEAAPRPARSRSRRGRGSKPAPEETPSAEETAPAAEAEAEAEAEA
HHHHHHCCCCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHCC
PVVEAAQEAAPEPVAEAEPEPEMAQADFAPPKAAAVEAEPAPEPEGAPAPSAAEPPAPKR
HHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCC
AGWWNRGKK
CCCCCCCCC
>Mature Secondary Structure 
SRLMLIDAAHPEETRVAIVNNGQVDDFDFEATGNEQLRGNIYLAKVTRVEPSLQAAFVE
CCEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEECCEEEEEEEECCHHHHHHHHH
YGGNRHGFLAFSEIHPDYYQLPAADRELLLKEAAEEAAAAIEDDDDGPYVPHGMSSEMGN
HCCCCCCEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
GASDEGDEDDDHGDDGDEDTASADDDGEDEGDDAAPRAAKPARRNAKKQTQRTPISKRYK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHH
IQEVIRRRQVMLVQVVKEERGNKGAALTTYLSLAGRYSVLMPNTPRGGGISRKIVNSADR
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHH
KRLKSIVSELDVPEGQGLIVRTAGAKRTKIEIKRDYDYLSKLWEQIVEKTLSSEAPALIN
HHHHHHHHHCCCCCCCCEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEE
AEGGLVHRAMRDMFDKEIEEVWVQGDEAYREAKDLAKIIMPSQAKKVQQWREEDPLYVAE
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHH
AVENQLDSIYSPVVQLKSGGYLVINQTEALVAIDVNSGKSTRERNIEQTAVRTNLEAAEE
HHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
ACRQMRLRDLAGLIVIDFIDMEENKNNRAVEKRLKECLKVDRARVQHGRISQFGLMEISR
HHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
QRRRQGVLQATSDPCSACNGTGRRRSIPSAALQLIRAIEARAATGGLKGIGVKAPTDVAL
HHHHCCCHHCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
YILNNKREALIEIERLAGFAISIHSSEDMLPGDFQIDADRDPNAKPKKRSTPRSLMKPTP
EEECCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCHHHCCCCC
PSARDDDDEEIDDEDVIEDEAGEDDDGETPAAAANKDKQGGGRRRRRRGGRNRGEARETG
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHC
LEVVDAEPAADADASEDDDDEDDGEDTSNGSAEAGSDDSDAARKRRRRGRRGGRRRRRGG
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCC
EGRGENGDIAAIADGGAFLDGLSVSADVMLSEDEPAIGLPAAADTQDTDDESETKAAPAE
CCCCCCCCEEEEECCCHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCHH
AAADAAETDEAPAATEEAAPRPARSRSRRGRGSKPAPEETPSAEETAPAAEAEAEAEAEA
HHHHHHCCCCCCCCCHHCCCCCHHHHHHCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHCC
PVVEAAQEAAPEPVAEAEPEPEMAQADFAPPKAAAVEAEPAPEPEGAPAPSAAEPPAPKR
HHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCC
AGWWNRGKK
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1447789; 1704367; 2011493; 8415644 [H]