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The map label for this gene is ispB [H]

Identifier: 114798232

GI number: 114798232

Start: 687463

End: 688476

Strand: Reverse

Name: ispB [H]

Synonym: HNE_0678

Alternate gene names: 114798232

Gene position: 688476-687463 (Counterclockwise)

Preceding gene: 114798485

Following gene: 114800079

Centisome position: 18.58

GC content: 64.3

Gene sequence:

>1014_bases
TTGACCCTCGCCCCAAAACCGAAGAGCAAGGCCCAGCTTTCCTCGATTACGGAGCGGATGCAGGCACTTGTCGCTGACGA
TCTGGCGGCCGTCGAACGCCTGCTGGCGACCCGCGCCGCCAGCCCTGTGGCAGTTATCCCGGATCTTTCAGGCTATATCG
TTTCGGCAGGCGGAAAGCGCCTGCGCCCGATGATCACCCTGATCGCCGCTCATGCTGTGGGCAAAGCCAATGCCGCGACC
CATGCGCTGGCCGCTGCGGTGGAGTTCATCCACACGGCGACCTTGCTGCATGATGACGTTGTGGACGAGAGCGACCTGCG
CCGGGGGAAACCCGCCGCGAAGGCGATCTGGGGCAACAAGGCCGCGATCCTGGTGGGCGACTTCCTGTTTGCGCGCGCAT
TCAACCTGCTGGTCGAAGCCAACAGCCTGGACATTCTCAACCGGCTGGCCACGGCCTCGACCGTGATTGCAGAGGGCGAA
GTGCGCCAGCTGGCAGCGATGGCAACCCGCGACCTGCCAACCGAAGAATATCTCGCCATTGTGGAAGCCAAGACGGGCGC
GCTGTTTGAAGCGGCGGCCGAAACCGGCGCGATGTCTGCGGGCGGAGAGGAATATTCCGACGCGCTGGCGACGTATGGCA
AGAATCTGGGCCTCGCCTTTCAGATCATTGATGATGCACTGGACTATGGTGGGACGACCTCTGTGATCGGCAAATCGGTG
GGCGATGATTTCCGCGAGGGGAAAATCACCCTGCCGGTGATCATTGCGCGGCGGCGGGGCTCTGACGAGGACCGGGCGTT
CTGGGACCGGGCGCTGAACCCGGAAACGCAGGAAGATTCCGACCTTGCCCATGCCGTGCACCTGATCCGCGCGACCGGGG
CGGCCGAAGCCACCGTGCAGGAAGCCGAAGCCTACGCCGCGCTGGCCAAGGGCGCGTTGCGCACCCTGCCCGCCTCGACC
TACCGGGACGCGCTGGAAGACCTGGCCGATTTCTGTGTAAGCCGGGCTTACTGA

Upstream 100 bases:

>100_bases
AGGCGGGCGGCTGCGGCGTGCGGCCCGGTGCCACGTCGAGCTTGGCTGGGTAATTGCGTAAATTTACTTATAACAAACTC
TTCCTGCCGGAGTGCTCCGT

Downstream 100 bases:

>100_bases
GTTATTTGTTCTCACTGGCAAGGTGGCGGCCGAGCGCGATGCGCTGCCAGCCCCAGAGCGCTGAACCTGCAAGGAACATG
AAGGCAAAGAGGGCGGCGGC

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MTLAPKPKSKAQLSSITERMQALVADDLAAVERLLATRAASPVAVIPDLSGYIVSAGGKRLRPMITLIAAHAVGKANAAT
HALAAAVEFIHTATLLHDDVVDESDLRRGKPAAKAIWGNKAAILVGDFLFARAFNLLVEANSLDILNRLATASTVIAEGE
VRQLAAMATRDLPTEEYLAIVEAKTGALFEAAAETGAMSAGGEEYSDALATYGKNLGLAFQIIDDALDYGGTTSVIGKSV
GDDFREGKITLPVIIARRRGSDEDRAFWDRALNPETQEDSDLAHAVHLIRATGAAEATVQEAEAYAALAKGALRTLPAST
YRDALEDLADFCVSRAY

Sequences:

>Translated_337_residues
MTLAPKPKSKAQLSSITERMQALVADDLAAVERLLATRAASPVAVIPDLSGYIVSAGGKRLRPMITLIAAHAVGKANAAT
HALAAAVEFIHTATLLHDDVVDESDLRRGKPAAKAIWGNKAAILVGDFLFARAFNLLVEANSLDILNRLATASTVIAEGE
VRQLAAMATRDLPTEEYLAIVEAKTGALFEAAAETGAMSAGGEEYSDALATYGKNLGLAFQIIDDALDYGGTTSVIGKSV
GDDFREGKITLPVIIARRRGSDEDRAFWDRALNPETQEDSDLAHAVHLIRATGAAEATVQEAEAYAALAKGALRTLPAST
YRDALEDLADFCVSRAY
>Mature_336_residues
TLAPKPKSKAQLSSITERMQALVADDLAAVERLLATRAASPVAVIPDLSGYIVSAGGKRLRPMITLIAAHAVGKANAATH
ALAAAVEFIHTATLLHDDVVDESDLRRGKPAAKAIWGNKAAILVGDFLFARAFNLLVEANSLDILNRLATASTVIAEGEV
RQLAAMATRDLPTEEYLAIVEAKTGALFEAAAETGAMSAGGEEYSDALATYGKNLGLAFQIIDDALDYGGTTSVIGKSVG
DDFREGKITLPVIIARRRGSDEDRAFWDRALNPETQEDSDLAHAVHLIRATGAAEATVQEAEAYAALAKGALRTLPASTY
RDALEDLADFCVSRAY

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=298, Percent_Identity=28.5234899328859, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4758430, Length=276, Percent_Identity=27.1739130434783, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI83700220, Length=276, Percent_Identity=27.1739130434783, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1789578, Length=319, Percent_Identity=44.8275862068966, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1786623, Length=268, Percent_Identity=34.3283582089552, Blast_Score=111, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17505681, Length=268, Percent_Identity=31.7164179104478, Blast_Score=135, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6319475, Length=303, Percent_Identity=29.3729372937294, Blast_Score=112, Evalue=9e-26,
Organism=Drosophila melanogaster, GI24651612, Length=330, Percent_Identity=29.6969696969697, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI281365769, Length=279, Percent_Identity=27.5985663082437, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24660002, Length=279, Percent_Identity=27.5985663082437, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35619; Mature: 35487

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAPKPKSKAQLSSITERMQALVADDLAAVERLLATRAASPVAVIPDLSGYIVSAGGKR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEECCCCCH
LRPMITLIAAHAVGKANAATHALAAAVEFIHTATLLHDDVVDESDLRRGKPAAKAIWGNK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHCCCC
AAILVGDFLFARAFNLLVEANSLDILNRLATASTVIAEGEVRQLAAMATRDLPTEEYLAI
EEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHH
VEAKTGALFEAAAETGAMSAGGEEYSDALATYGKNLGLAFQIIDDALDYGGTTSVIGKSV
HCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHH
GDDFREGKITLPVIIARRRGSDEDRAFWDRALNPETQEDSDLAHAVHLIRATGAAEATVQ
CCHHHCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
EAEAYAALAKGALRTLPASTYRDALEDLADFCVSRAY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLAPKPKSKAQLSSITERMQALVADDLAAVERLLATRAASPVAVIPDLSGYIVSAGGKR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEECCCCCH
LRPMITLIAAHAVGKANAATHALAAAVEFIHTATLLHDDVVDESDLRRGKPAAKAIWGNK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHCCCC
AAILVGDFLFARAFNLLVEANSLDILNRLATASTVIAEGEVRQLAAMATRDLPTEEYLAI
EEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHH
VEAKTGALFEAAAETGAMSAGGEEYSDALATYGKNLGLAFQIIDDALDYGGTTSVIGKSV
HCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHH
GDDFREGKITLPVIIARRRGSDEDRAFWDRALNPETQEDSDLAHAVHLIRATGAAEATVQ
CCHHHCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHH
EAEAYAALAKGALRTLPASTYRDALEDLADFCVSRAY
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]