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The map label for this gene is fabI2 [H]

Identifier: 114799930

GI number: 114799930

Start: 3575314

End: 3576132

Strand: Direct

Name: fabI2 [H]

Synonym: HNE_3436

Alternate gene names: 114799930

Gene position: 3575314-3576132 (Clockwise)

Preceding gene: 114800283

Following gene: 114798694

Centisome position: 96.5

GC content: 64.59

Gene sequence:

>819_bases
ATGGCCGACGACTGGAACATGCCCAAAGGCGACCTGATGAAGGGCAAGCGCGGCGTCGTCATGGGCGTTGCCAACCAGAA
CTCGATCGCCTGGGGCATCTCCACCCAGCTTGCTGCCCAGGGCGCCGAGATTGCCTTCACCTATCAGGGCGAAAGCCTGG
AGCGGCGCGTGCGCCCCCTCGTCGAATCAATCGGCATGGACACGATGATTCCGGCGGATGTGACCGATGACGCCTCGATG
GACGCCGCCTTTGCCGCCATCAAGGAAAAATGGGGCAAGATCGATTTCCTTGTCCACTCCATCGCCTTTGCCGGGAAAGA
TGAACTGCAAGGCTCGATGGTTGCCAACACCACCCGCGAAGGCTTCCGCCGCGCGATGGACATCTCCGTATACAGCTTTA
TTGACTGCGCCCGCCGCGCCTCGGAAATCATGCCGGATGGTGGCTCGATCATCTGCATGACGTATCTCGGCGCCGAGCGC
ACCGTGCCGTCGTACAATGTCATGGGCGTTGCCAAGGCGGCGCTGGAAGCCTCCACCCGCTATGCCGCGCGCGACCTTGG
CCCCCAGGGCATCCGCGTCAACGCCATCTCCGCCGGCGCCATGCGCACGCTCTCGCTCGCGGGCATCCGCGGCGGCAAGA
GCCTGATGGGCACGGGCCGCAGCTGGTCGCTTCTGAAGGAAGACACCAGCATGGAAGGCGTCGCGGGTTGCGCGCTCTAC
CTCCTCTCCCCCCTCGGCCGCTCCATCGCGGGCGAAGTGATCCACGTTGACGCCGGCTTCCACGTCGTCGGCGTGCCGGA
TGCGGGCGAGGAAGAGTAA

Upstream 100 bases:

>100_bases
CGGCGGCGCGGGCGATCTGCCCGCTTCTTGACGAGGGCTTCCGGCGCGCCTAGCCCGGATGCCAGACAAACACACAGCCA
CCATCAGGGAGTGAGCAATC

Downstream 100 bases:

>100_bases
GCTTTCTGGCCAATCTTGACTTCAGGGCGAGGTAATCCGAAAAGGATACCTCGCCCTTTGAGGTTTTTATGTTCCGTACC
CTGTCTGAGTCCCGCTGAAG

Product: enoyl-[acyl-carrier-protein] reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MADDWNMPKGDLMKGKRGVVMGVANQNSIAWGISTQLAAQGAEIAFTYQGESLERRVRPLVESIGMDTMIPADVTDDASM
DAAFAAIKEKWGKIDFLVHSIAFAGKDELQGSMVANTTREGFRRAMDISVYSFIDCARRASEIMPDGGSIICMTYLGAER
TVPSYNVMGVAKAALEASTRYAARDLGPQGIRVNAISAGAMRTLSLAGIRGGKSLMGTGRSWSLLKEDTSMEGVAGCALY
LLSPLGRSIAGEVIHVDAGFHVVGVPDAGEEE

Sequences:

>Translated_272_residues
MADDWNMPKGDLMKGKRGVVMGVANQNSIAWGISTQLAAQGAEIAFTYQGESLERRVRPLVESIGMDTMIPADVTDDASM
DAAFAAIKEKWGKIDFLVHSIAFAGKDELQGSMVANTTREGFRRAMDISVYSFIDCARRASEIMPDGGSIICMTYLGAER
TVPSYNVMGVAKAALEASTRYAARDLGPQGIRVNAISAGAMRTLSLAGIRGGKSLMGTGRSWSLLKEDTSMEGVAGCALY
LLSPLGRSIAGEVIHVDAGFHVVGVPDAGEEE
>Mature_271_residues
ADDWNMPKGDLMKGKRGVVMGVANQNSIAWGISTQLAAQGAEIAFTYQGESLERRVRPLVESIGMDTMIPADVTDDASMD
AAFAAIKEKWGKIDFLVHSIAFAGKDELQGSMVANTTREGFRRAMDISVYSFIDCARRASEIMPDGGSIICMTYLGAERT
VPSYNVMGVAKAALEASTRYAARDLGPQGIRVNAISAGAMRTLSLAGIRGGKSLMGTGRSWSLLKEDTSMEGVAGCALYL
LSPLGRSIAGEVIHVDAGFHVVGVPDAGEEE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=251, Percent_Identity=44.6215139442231, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI1789378, Length=254, Percent_Identity=22.8346456692913, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17560332, Length=186, Percent_Identity=31.1827956989247, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562906, Length=195, Percent_Identity=28.2051282051282, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=191, Percent_Identity=28.2722513089005, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357041, Length=135, Percent_Identity=31.8518518518519, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28906; Mature: 28775

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADDWNMPKGDLMKGKRGVVMGVANQNSIAWGISTQLAAQGAEIAFTYQGESLERRVRPL
CCCCCCCCCHHHCCCCCCEEEEECCCCCEEECCHHHHHHCCCEEEEEECCHHHHHHHHHH
VESIGMDTMIPADVTDDASMDAAFAAIKEKWGKIDFLVHSIAFAGKDELQGSMVANTTRE
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHCCCHHHHHHHH
GFRRAMDISVYSFIDCARRASEIMPDGGSIICMTYLGAERTVPSYNVMGVAKAALEASTR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
YAARDLGPQGIRVNAISAGAMRTLSLAGIRGGKSLMGTGRSWSLLKEDTSMEGVAGCALY
HHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHCCCCCCCHHHCCCCCCHHHHHHHH
LLSPLGRSIAGEVIHVDAGFHVVGVPDAGEEE
HHHHHHHHHCCCEEEEECCEEEEECCCCCCCC
>Mature Secondary Structure 
ADDWNMPKGDLMKGKRGVVMGVANQNSIAWGISTQLAAQGAEIAFTYQGESLERRVRPL
CCCCCCCCHHHCCCCCCEEEEECCCCCEEECCHHHHHHCCCEEEEEECCHHHHHHHHHH
VESIGMDTMIPADVTDDASMDAAFAAIKEKWGKIDFLVHSIAFAGKDELQGSMVANTTRE
HHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHCCCHHHHHHHH
GFRRAMDISVYSFIDCARRASEIMPDGGSIICMTYLGAERTVPSYNVMGVAKAALEASTR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
YAARDLGPQGIRVNAISAGAMRTLSLAGIRGGKSLMGTGRSWSLLKEDTSMEGVAGCALY
HHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCHHHHCCCCCCCHHHCCCCCCHHHHHHHH
LLSPLGRSIAGEVIHVDAGFHVVGVPDAGEEE
HHHHHHHHHCCCEEEEECCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]