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The map label for this gene is ddl

Identifier: 114799909

GI number: 114799909

Start: 387251

End: 388171

Strand: Reverse

Name: ddl

Synonym: HNE_0393

Alternate gene names: 114799909

Gene position: 388171-387251 (Counterclockwise)

Preceding gene: 114799112

Following gene: 114798641

Centisome position: 10.48

GC content: 64.82

Gene sequence:

>921_bases
ATGAAGCGGGACACGAAACGGGTAGCAGTGATTTATGGCGGCTGGTCTTCGGAACGGCCGGTCTCCCTGTCTTCCGGCCA
GCAGATGGCCGCCGCCGCCCGCAAGGCCGGCTATGACGTGACCGAGATCGACGTGACCCGTGAACTGGCGAGCCAGCTGG
CAGAGGCCGAGCCCGATGTGGTGCTCAACGGGCTTCATGGCCCCTGGGGCGAGGATGGCTGCGTGCAGGGCCTGCTCGAA
GTGATGGGCCTGCCCTACTCCCATTCCGGCGTGCTCGCGTCTGCCCTCGCCATGGACAAGCTCAAATCCAAAGCCGTCTA
TCGCAGCGCCGGTCTCGATGTTGCCGCTGACAAGCGCGTCACCCGCGCAGAGGCCGCCGCCGCCCATGCCCTGCCCCCGC
CTTACGTGGTCAAGCCGGTGAACGAAGGCTCCAGCTTCGGCGTGCTGATCGTGCGCGAAGGCGCCAACGGCCCGCCTCAC
GAGCTGACCGGCCCGAACTGGCGCTATGGCGATTATCTGATGGCGGAAGAATACATCCCCGGCCGCGAGCTGACCGTCGC
CGTGCTGGGCGACCGGGCCCTGGCTGTCACGGAAATCACGACCTTAAGGGACTATTACGATTTTGATGCAAAATACGCGG
CTGGTGGATCAAGGCATGTGATCCCGGCAGACTTGCCCGCGAAAGTGACGGAGGCGGCGATGGACGCCGCGCTGCTCGCG
CACCGGGCGCTTGGCTGTCGCGGCGCTACACGCTCGGACTTCCGCTATGACGAACAGAAGGACCGGCTCGTGATCCTCGA
AACCAATACCCAGCCCGGCATGACGCCGACCTCGCTCGTGCCCGAGCAGGCCGCGCATATGGGCATGAGTTTCGAAGACC
TCGTCGCCTGGATGATCGAGGACGCCTCATGCCAAAGATAG

Upstream 100 bases:

>100_bases
CCTGACAGCCGCCCGCCCCTGCGTCTTAAGTCGGTATTCACCACGACACGGCATCAAGGCATGCGAGACGCGGCAGTAGG
CCGGCAGGAGGAGATTTCCC

Downstream 100 bases:

>100_bases
AGCGTAACCAGACTGTTCCCGGCCGCCGCCGCGCGCCCGCCACCATCATTGACGAGACCACCGGCGAGGAAGTGCGCGTT
TCCTCGGTGATTTTCGGCAT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKRDTKRVAVIYGGWSSERPVSLSSGQQMAAAARKAGYDVTEIDVTRELASQLAEAEPDVVLNGLHGPWGEDGCVQGLLE
VMGLPYSHSGVLASALAMDKLKSKAVYRSAGLDVAADKRVTRAEAAAAHALPPPYVVKPVNEGSSFGVLIVREGANGPPH
ELTGPNWRYGDYLMAEEYIPGRELTVAVLGDRALAVTEITTLRDYYDFDAKYAAGGSRHVIPADLPAKVTEAAMDAALLA
HRALGCRGATRSDFRYDEQKDRLVILETNTQPGMTPTSLVPEQAAHMGMSFEDLVAWMIEDASCQR

Sequences:

>Translated_306_residues
MKRDTKRVAVIYGGWSSERPVSLSSGQQMAAAARKAGYDVTEIDVTRELASQLAEAEPDVVLNGLHGPWGEDGCVQGLLE
VMGLPYSHSGVLASALAMDKLKSKAVYRSAGLDVAADKRVTRAEAAAAHALPPPYVVKPVNEGSSFGVLIVREGANGPPH
ELTGPNWRYGDYLMAEEYIPGRELTVAVLGDRALAVTEITTLRDYYDFDAKYAAGGSRHVIPADLPAKVTEAAMDAALLA
HRALGCRGATRSDFRYDEQKDRLVILETNTQPGMTPTSLVPEQAAHMGMSFEDLVAWMIEDASCQR
>Mature_306_residues
MKRDTKRVAVIYGGWSSERPVSLSSGQQMAAAARKAGYDVTEIDVTRELASQLAEAEPDVVLNGLHGPWGEDGCVQGLLE
VMGLPYSHSGVLASALAMDKLKSKAVYRSAGLDVAADKRVTRAEAAAAHALPPPYVVKPVNEGSSFGVLIVREGANGPPH
ELTGPNWRYGDYLMAEEYIPGRELTVAVLGDRALAVTEITTLRDYYDFDAKYAAGGSRHVIPADLPAKVTEAAMDAALLA
HRALGCRGATRSDFRYDEQKDRLVILETNTQPGMTPTSLVPEQAAHMGMSFEDLVAWMIEDASCQR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=309, Percent_Identity=41.1003236245955, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1786579, Length=348, Percent_Identity=31.3218390804598, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_HYPNA (Q0C570)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759123.1
- ProteinModelPortal:   Q0C570
- SMR:   Q0C570
- STRING:   Q0C570
- GeneID:   4290239
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0393
- NMPDR:   fig|228405.5.peg.387
- TIGR:   HNE_0393
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- PhylomeDB:   Q0C570
- ProtClustDB:   CLSK2532079
- BioCyc:   HNEP81032:HNE_0393-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32960; Mature: 32960

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRDTKRVAVIYGGWSSERPVSLSSGQQMAAAARKAGYDVTEIDVTRELASQLAEAEPDV
CCCCCEEEEEEEECCCCCCCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCE
VLNGLHGPWGEDGCVQGLLEVMGLPYSHSGVLASALAMDKLKSKAVYRSAGLDVAADKRV
EEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHH
TRAEAAAAHALPPPYVVKPVNEGSSFGVLIVREGANGPPHELTGPNWRYGDYLMAEEYIP
HHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCC
GRELTVAVLGDRALAVTEITTLRDYYDFDAKYAAGGSRHVIPADLPAKVTEAAMDAALLA
CCCEEEEEECCCEEEEHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHH
HRALGCRGATRSDFRYDEQKDRLVILETNTQPGMTPTSLVPEQAAHMGMSFEDLVAWMIE
HHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHCHHHHHHCCCCHHHHHHHHHH
DASCQR
HCCCCC
>Mature Secondary Structure
MKRDTKRVAVIYGGWSSERPVSLSSGQQMAAAARKAGYDVTEIDVTRELASQLAEAEPDV
CCCCCEEEEEEEECCCCCCCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCE
VLNGLHGPWGEDGCVQGLLEVMGLPYSHSGVLASALAMDKLKSKAVYRSAGLDVAADKRV
EEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHH
TRAEAAAAHALPPPYVVKPVNEGSSFGVLIVREGANGPPHELTGPNWRYGDYLMAEEYIP
HHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCC
GRELTVAVLGDRALAVTEITTLRDYYDFDAKYAAGGSRHVIPADLPAKVTEAAMDAALLA
CCCEEEEEECCCEEEEHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHH
HRALGCRGATRSDFRYDEQKDRLVILETNTQPGMTPTSLVPEQAAHMGMSFEDLVAWMIE
HHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHCHHHHHHCCCCHHHHHHHHHH
DASCQR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA