Click here to switch to the map view.

The map label for this gene is yxeQ [H]

Identifier: 114799908

GI number: 114799908

Start: 1108826

End: 1110133

Strand: Direct

Name: yxeQ [H]

Synonym: HNE_1103

Alternate gene names: 114799908

Gene position: 1108826-1110133 (Clockwise)

Preceding gene: 114798181

Following gene: 114797367

Centisome position: 29.93

GC content: 64.83

Gene sequence:

>1308_bases
ATGTCCGGACAAACTAACGTGCCTTTCCAGACTTCGCAAACCCTTACGCAGGCGTTGGCCGGGCGGATCGCCGGACATGT
CTCGAAGGCGGACCGTGCACGGGCGCAGTGGCATCTGCTGGACTGGGCAGGTTGCGCGGTGGCCGGGCGCAGCGAGCCTG
CCGGCCGCATCCTGCTGGCGGGGCTGGAGCCTTCGCCCAGCGCGCGGGCCTTTGCCTGGGGCGGGCTGGGAAACATTCTC
GAAATGGATGATGTGGACAAGCGCGCGCTGCTGCATCCTGGCCCCAGCATCATTCCGGCGGCGCTCGCCTGCGCAGCAGA
AAACACTGTTTCGGGTGAGGCGCTGCTGGATGCAATCGTGCGCGGTTATGAGGCGACGATCCGGCTGGGTCGCGCGGTTG
GGCCGGGGCATTATGCGCTGTGGCACAATACCGGCACCTGCGGCGCGATCGGCGCGGCGGCGGCCTGTGCAAGCCTGATG
GAACTGGACACCGAAGCAACTGCCCATGCGCTGGCATTGGCGGTCAGTCAGTCGGCGGGGCTCTGGCAAACGCGGCATGA
GCCCGCCAGTATGGGCAAGCAGTTACATACGTCGGTTTCGTCGCGCGCAGGTTTTGATGCCGCGCGGCTGGCAGCGGCGG
GATTCAAAGGGCCGCTTTCAATTCTGGAGGGGCCACAGGGCTTCTTCGCGGCGATGTGCCCCGGCGCTGATCCTGCCGCG
GTTATGGCAGGAGAGGATGGCTGGCGCATCCATGATGTCAGCTTCAAGCCCTGGCCTGCCTGCCGGCATGCGCATGCTGT
GATTGATGCGGCGCTGGCGCTGCGTGCGCGCGTGAATGTGTTGGATACCGGCGGCGACATCCTCGTCGAAACATACCGCG
ACGCGCTCGTTTTCTGTGACAAGCCGGAGCCGGAATCGGTGATCGAGGCGAAGTTTTCGCTGCAGCATTCTGTGGCGGTG
GTGATGGTGCGGGGGCGGCCGGAACTGGCGGATTTCAGCCTGGATTCGGTGGCTGATCCGGCGATCACGGCGATGCGAAA
CCGGGTCAAAGTTGGACAAAACGGGACATTTGACGCCAATTACCCGTCACGCTTTGGCGCTTCAGTGACCTTCGCGGGAC
ACACCGAGACAGTTCCGGACGCCCTGGGTGATCCGGAGAACCCGGTCGGATTGGATCAGCTTCGCGACAAGGCGCGCGCG
CTGCTGGCGGCGGGTGGTTTGAGCGAAACGGAAACGGCGGAGCTTATGGCGGCGGCGGAAACGGCCTCGCCCGAACTGGG
CGGTCTGATCCTCAAGGTGCTGGCATGA

Upstream 100 bases:

>100_bases
AGGGCAGGGCTGGTAAGAAAGCAAAAGATATATCAAATAAATGCCTCGAAGGGTCTGAAAGCGGTTTTCGACTGCCATTT
GGTCATGCTAAGCAGGTTAT

Downstream 100 bases:

>100_bases
GCGGAGCGGCGGAAGCCTTTGTGCGCCATACCCTCACCCTCCGCTGGGAGGCGATGCCCGAGGCGGCGCAAGGGGCGGTG
AAGACGTTCCTGCTCGACAG

Product: MmgE/PrpD family protein

Products: cis-2-Methylaconitate; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSGQTNVPFQTSQTLTQALAGRIAGHVSKADRARAQWHLLDWAGCAVAGRSEPAGRILLAGLEPSPSARAFAWGGLGNIL
EMDDVDKRALLHPGPSIIPAALACAAENTVSGEALLDAIVRGYEATIRLGRAVGPGHYALWHNTGTCGAIGAAAACASLM
ELDTEATAHALALAVSQSAGLWQTRHEPASMGKQLHTSVSSRAGFDAARLAAAGFKGPLSILEGPQGFFAAMCPGADPAA
VMAGEDGWRIHDVSFKPWPACRHAHAVIDAALALRARVNVLDTGGDILVETYRDALVFCDKPEPESVIEAKFSLQHSVAV
VMVRGRPELADFSLDSVADPAITAMRNRVKVGQNGTFDANYPSRFGASVTFAGHTETVPDALGDPENPVGLDQLRDKARA
LLAAGGLSETETAELMAAAETASPELGGLILKVLA

Sequences:

>Translated_435_residues
MSGQTNVPFQTSQTLTQALAGRIAGHVSKADRARAQWHLLDWAGCAVAGRSEPAGRILLAGLEPSPSARAFAWGGLGNIL
EMDDVDKRALLHPGPSIIPAALACAAENTVSGEALLDAIVRGYEATIRLGRAVGPGHYALWHNTGTCGAIGAAAACASLM
ELDTEATAHALALAVSQSAGLWQTRHEPASMGKQLHTSVSSRAGFDAARLAAAGFKGPLSILEGPQGFFAAMCPGADPAA
VMAGEDGWRIHDVSFKPWPACRHAHAVIDAALALRARVNVLDTGGDILVETYRDALVFCDKPEPESVIEAKFSLQHSVAV
VMVRGRPELADFSLDSVADPAITAMRNRVKVGQNGTFDANYPSRFGASVTFAGHTETVPDALGDPENPVGLDQLRDKARA
LLAAGGLSETETAELMAAAETASPELGGLILKVLA
>Mature_434_residues
SGQTNVPFQTSQTLTQALAGRIAGHVSKADRARAQWHLLDWAGCAVAGRSEPAGRILLAGLEPSPSARAFAWGGLGNILE
MDDVDKRALLHPGPSIIPAALACAAENTVSGEALLDAIVRGYEATIRLGRAVGPGHYALWHNTGTCGAIGAAAACASLME
LDTEATAHALALAVSQSAGLWQTRHEPASMGKQLHTSVSSRAGFDAARLAAAGFKGPLSILEGPQGFFAAMCPGADPAAV
MAGEDGWRIHDVSFKPWPACRHAHAVIDAALALRARVNVLDTGGDILVETYRDALVFCDKPEPESVIEAKFSLQHSVAVV
MVRGRPELADFSLDSVADPAITAMRNRVKVGQNGTFDANYPSRFGASVTFAGHTETVPDALGDPENPVGLDQLRDKARAL
LAAGGLSETETAELMAAAETASPELGGLILKVLA

Specific function: Catalyzes The Dehydration Of 2-Methylcitrate To 2- Methyl-Cis-Aconitate. Also Seems To Be Responsible For The Residual Aconitase Activity Of The Acnab-Null Strain. [C]

COG id: COG2079

COG function: function code R; Uncharacterized protein involved in propionate catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpD family [H]

Homologues:

Organism=Homo sapiens, GI310129732, Length=387, Percent_Identity=24.031007751938, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI310110533, Length=387, Percent_Identity=24.031007751938, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005656 [H]

Pfam domain/function: PF03972 MmgE_PrpD [H]

EC number: 4.2.1.79 [C]

Molecular weight: Translated: 45268; Mature: 45136

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQTNVPFQTSQTLTQALAGRIAGHVSKADRARAQWHLLDWAGCAVAGRSEPAGRILLA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEECCCCCCCCEEEEE
GLEPSPSARAFAWGGLGNILEMDDVDKRALLHPGPSIIPAALACAAENTVSGEALLDAIV
ECCCCCCCCEEEECCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
RGYEATIRLGRAVGPGHYALWHNTGTCGAIGAAAACASLMELDTEATAHALALAVSQSAG
HCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
LWQTRHEPASMGKQLHTSVSSRAGFDAARLAAAGFKGPLSILEGPQGFFAAMCPGADPAA
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCCCCCEEEECCCCCCCE
VMAGEDGWRIHDVSFKPWPACRHAHAVIDAALALRARVNVLDTGGDILVETYRDALVFCD
EEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCEEHEEHHCEEEEEC
KPEPESVIEAKFSLQHSVAVVMVRGRPELADFSLDSVADPAITAMRNRVKVGQNGTFDAN
CCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCC
YPSRFGASVTFAGHTETVPDALGDPENPVGLDQLRDKARALLAAGGLSETETAELMAAAE
CCHHHCCEEEECCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TASPELGGLILKVLA
CCCCHHHHHHHHHCC
>Mature Secondary Structure 
SGQTNVPFQTSQTLTQALAGRIAGHVSKADRARAQWHLLDWAGCAVAGRSEPAGRILLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCEEECCCCCCCCEEEEE
GLEPSPSARAFAWGGLGNILEMDDVDKRALLHPGPSIIPAALACAAENTVSGEALLDAIV
ECCCCCCCCEEEECCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
RGYEATIRLGRAVGPGHYALWHNTGTCGAIGAAAACASLMELDTEATAHALALAVSQSAG
HCHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
LWQTRHEPASMGKQLHTSVSSRAGFDAARLAAAGFKGPLSILEGPQGFFAAMCPGADPAA
CCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHCCCCCCEEEECCCCCCCE
VMAGEDGWRIHDVSFKPWPACRHAHAVIDAALALRARVNVLDTGGDILVETYRDALVFCD
EEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCEEHEEHHCEEEEEC
KPEPESVIEAKFSLQHSVAVVMVRGRPELADFSLDSVADPAITAMRNRVKVGQNGTFDAN
CCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCC
YPSRFGASVTFAGHTETVPDALGDPENPVGLDQLRDKARALLAAGGLSETETAELMAAAE
CCHHHCCEEEECCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TASPELGGLILKVLA
CCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Methylcitrate [C]

Specific reaction: 2-Methylcitrate --> cis-2-Methylaconitate + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]