Click here to switch to the map view.

The map label for this gene is dnaA [H]

Identifier: 114799907

GI number: 114799907

Start: 561363

End: 562736

Strand: Reverse

Name: dnaA [H]

Synonym: HNE_0564

Alternate gene names: 114799907

Gene position: 562736-561363 (Counterclockwise)

Preceding gene: 114800507

Following gene: 114798873

Centisome position: 15.19

GC content: 63.46

Gene sequence:

>1374_bases
ATGATTTGGAACGATGTGCGCGATGTCCTGCGGGACAAGATCACGGAATCTGAATTCACGCGCTGGATCGCAGACCTGCG
CGTTGTCGCAGAACACAATGGCGAAATGATTGTCGCCGCGCGCGATCCGCTGGCTTTTGACCGCGTCAGCAGCCAGCATC
TGCGCGTGATCGAGAAATTGTGGGCAGCCGCTGACCCGGCTGAACGCGCCCTCAAATTGATCTGTTGGCGGAATGCTCCG
GCAGACCTGCGCGATCTGGTCGATGACCCATGGGCTGTTGCCGAGACGCCGGTTCCGGCCGAGCCGCAGGCACCCGCCAC
ATCGTCGGGCGCTGGCCCGGCAATGACCTTCGACACGCTGGTGACCGGCCCGTCGAACGAAATCGCCGTGACGCTGGCAA
AGCGTATTGCCGCGGGCCTTCCTGCCGGCACGGCCACCACGCTGATCTATGGCCCGCCCGGCACCGGCAAGACCCACCTC
ATGCAGGCCCTCCGTCTGGAAGCCGCCCGCCGGGACACTGCCCGCCGGATCGTCTACCTGACCGCCGAGGAATTCATGTC
GGCCTATCTGGACGGTGTGAAGGCACGCGACACGAGCGACCTGAAGAAGCGTCTGCGGGCCGCCTCGATCCTGCTGATCG
ACGACCTGCACCGCATCGCCGGCAAACCCGGCACGGAAACCGAGCTCTATCAGAACATCCGCGAAGTCACGTCCAATGGC
GGGCAGGTTGTTCTGGTCGGCGACTCTGCCCCAGGCGATGCCTCCGGTTTCGGCCAGCGGATGCGCAGCGAGATCAAGGG
CGCGACTGCCGTTGAGGTGGAACTGCCAGATGCCGGCATGCGCCGCGAAATCCTGATCCGCCTGGCCGGCCACATCGCGG
AAAATCATCCCGCCTTCGTGCTGACCGATGAGATGATGCAGCGTCTCAACTCCGGCATCCGTGGCCCCGGCCGCGAGCTG
ACCGGCGCGGTGTGGAGCCTCTACACCGAAGCAGGCTTCGGTGAGCGGGCGCCAACAATGGAAATGCTGGACCGCATTGT
CCGCCGTCATGCCGGCGAGCAGCGCGAGCCCTCGATCGATGTAATCAAGCGGGCGACGCTGAAAGTGTTCCCGATTGCCA
AGACCGATCTGGAAGGCCCGAGCAAGATGCGCGGCTTCGTCTACCCCCGTCAGATTGCGATGTATCTCTGCCGGACGATG
ACGCGGAAATCCTTCCCTCAGATCGGCCGGGCCTTTGGCAAACGCGACCACACAACGGTCCTTTACGCCTATCGCCGCAT
GGATGGGGACATCGGCAAGGACCAGGAACTGGCCGCAGACATTGCCCGCGTTCAGACTGTGGTGCTTGAGCTGATGGAAA
GCGGACAAATCTGA

Upstream 100 bases:

>100_bases
TTAGAAACGGGGCTCGGTGATGGACCGAAACGAAACAACTGTCTCGCCTTTCGAATTGGAGGCGGCACAAGGCAGTGACG
CTTTTGAAACGACAGGCCAA

Downstream 100 bases:

>100_bases
GCCATTTTCAGCGCGCGGGCTGCCGGGCGCTTGGCTTTTCCTGCAACTTCGTTAAGGCTCATCAGCCCGCTCCTGCCCGG
AGCGGGCTGATTCCCGGCTT

Product: chromosomal replication initiator protein DnaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MIWNDVRDVLRDKITESEFTRWIADLRVVAEHNGEMIVAARDPLAFDRVSSQHLRVIEKLWAAADPAERALKLICWRNAP
ADLRDLVDDPWAVAETPVPAEPQAPATSSGAGPAMTFDTLVTGPSNEIAVTLAKRIAAGLPAGTATTLIYGPPGTGKTHL
MQALRLEAARRDTARRIVYLTAEEFMSAYLDGVKARDTSDLKKRLRAASILLIDDLHRIAGKPGTETELYQNIREVTSNG
GQVVLVGDSAPGDASGFGQRMRSEIKGATAVEVELPDAGMRREILIRLAGHIAENHPAFVLTDEMMQRLNSGIRGPGREL
TGAVWSLYTEAGFGERAPTMEMLDRIVRRHAGEQREPSIDVIKRATLKVFPIAKTDLEGPSKMRGFVYPRQIAMYLCRTM
TRKSFPQIGRAFGKRDHTTVLYAYRRMDGDIGKDQELAADIARVQTVVLELMESGQI

Sequences:

>Translated_457_residues
MIWNDVRDVLRDKITESEFTRWIADLRVVAEHNGEMIVAARDPLAFDRVSSQHLRVIEKLWAAADPAERALKLICWRNAP
ADLRDLVDDPWAVAETPVPAEPQAPATSSGAGPAMTFDTLVTGPSNEIAVTLAKRIAAGLPAGTATTLIYGPPGTGKTHL
MQALRLEAARRDTARRIVYLTAEEFMSAYLDGVKARDTSDLKKRLRAASILLIDDLHRIAGKPGTETELYQNIREVTSNG
GQVVLVGDSAPGDASGFGQRMRSEIKGATAVEVELPDAGMRREILIRLAGHIAENHPAFVLTDEMMQRLNSGIRGPGREL
TGAVWSLYTEAGFGERAPTMEMLDRIVRRHAGEQREPSIDVIKRATLKVFPIAKTDLEGPSKMRGFVYPRQIAMYLCRTM
TRKSFPQIGRAFGKRDHTTVLYAYRRMDGDIGKDQELAADIARVQTVVLELMESGQI
>Mature_457_residues
MIWNDVRDVLRDKITESEFTRWIADLRVVAEHNGEMIVAARDPLAFDRVSSQHLRVIEKLWAAADPAERALKLICWRNAP
ADLRDLVDDPWAVAETPVPAEPQAPATSSGAGPAMTFDTLVTGPSNEIAVTLAKRIAAGLPAGTATTLIYGPPGTGKTHL
MQALRLEAARRDTARRIVYLTAEEFMSAYLDGVKARDTSDLKKRLRAASILLIDDLHRIAGKPGTETELYQNIREVTSNG
GQVVLVGDSAPGDASGFGQRMRSEIKGATAVEVELPDAGMRREILIRLAGHIAENHPAFVLTDEMMQRLNSGIRGPGREL
TGAVWSLYTEAGFGERAPTMEMLDRIVRRHAGEQREPSIDVIKRATLKVFPIAKTDLEGPSKMRGFVYPRQIAMYLCRTM
TRKSFPQIGRAFGKRDHTTVLYAYRRMDGDIGKDQELAADIARVQTVVLELMESGQI

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=489, Percent_Identity=27.4028629856851, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 50405; Mature: 50405

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWNDVRDVLRDKITESEFTRWIADLRVVAEHNGEMIVAARDPLAFDRVSSQHLRVIEKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCHHHHHHHHHHHHHHHH
WAAADPAERALKLICWRNAPADLRDLVDDPWAVAETPVPAEPQAPATSSGAGPAMTFDTL
HHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHE
VTGPSNEIAVTLAKRIAAGLPAGTATTLIYGPPGTGKTHLMQALRLEAARRDTARRIVYL
ECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEE
TAEEFMSAYLDGVKARDTSDLKKRLRAASILLIDDLHRIAGKPGTETELYQNIREVTSNG
EHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GQVVLVGDSAPGDASGFGQRMRSEIKGATAVEVELPDAGMRREILIRLAGHIAENHPAFV
CEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEE
LTDEMMQRLNSGIRGPGRELTGAVWSLYTEAGFGERAPTMEMLDRIVRRHAGEQREPSID
EHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHH
VIKRATLKVFPIAKTDLEGPSKMRGFVYPRQIAMYLCRTMTRKSFPQIGRAFGKRDHTTV
HHHHHHEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LYAYRRMDGDIGKDQELAADIARVQTVVLELMESGQI
EEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIWNDVRDVLRDKITESEFTRWIADLRVVAEHNGEMIVAARDPLAFDRVSSQHLRVIEKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCHHHHHHHHHHHHHHHH
WAAADPAERALKLICWRNAPADLRDLVDDPWAVAETPVPAEPQAPATSSGAGPAMTFDTL
HHCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHE
VTGPSNEIAVTLAKRIAAGLPAGTATTLIYGPPGTGKTHLMQALRLEAARRDTARRIVYL
ECCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEE
TAEEFMSAYLDGVKARDTSDLKKRLRAASILLIDDLHRIAGKPGTETELYQNIREVTSNG
EHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GQVVLVGDSAPGDASGFGQRMRSEIKGATAVEVELPDAGMRREILIRLAGHIAENHPAFV
CEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEE
LTDEMMQRLNSGIRGPGRELTGAVWSLYTEAGFGERAPTMEMLDRIVRRHAGEQREPSID
EHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHH
VIKRATLKVFPIAKTDLEGPSKMRGFVYPRQIAMYLCRTMTRKSFPQIGRAFGKRDHTTV
HHHHHHEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LYAYRRMDGDIGKDQELAADIARVQTVVLELMESGQI
EEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA