Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 114798873

GI number: 114798873

Start: 560058

End: 561179

Strand: Reverse

Name: dnaN [H]

Synonym: HNE_0563

Alternate gene names: 114798873

Gene position: 561179-560058 (Counterclockwise)

Preceding gene: 114799907

Following gene: 114800522

Centisome position: 15.15

GC content: 63.01

Gene sequence:

>1122_bases
ATGAAACTGACGATCGAACGCGGAGACCTTCTGGACGCCCTCAGCCATGTTCAGAACATTGTCGAGCGCCGGACGACAAT
TCCAATTCTCTCCAACGTTTTGCTGGAGGCAACCCAGGGCGCGCTGAAGCTCACCGCAACCGACCTCGACATCGAGGCCG
TGGACTCTGCGCTCGCCAATGTGTCCCGCCCGGGCTCAGTAACGGCTCCGGCCGGCACGCTGTTTGACGTGGTGCGCAAG
CTGCCCTCCGGCGCTGAGGTGGAGCTGGAGCTCAACCCGGAGTCCCAGCGCCTGACGATCGCCGCCGGCCGCTCACTGTT
CGAACTGCCAACCCTGCCGGCAGCTGACTTCCAGACCATGACGCCCGATGAAGGCGCCGTCAGCTTCAAGATCGCCGCGA
AGGATTTCGCCCGCCTGATCGACAAGACCCGCTTTGCGATCTCCACCGAAGAGACCCGCTACTATCTCAACGGCATCTAT
CTGCACGCCGCCAAGGCCGATGATGGCTCCGCCGTGCTGCGTACCGTCGCCACCGATGGCCACCGCCTCGCCCTGGCGGA
GCTGAAAGCCCCCAAGGGCGCCGAGAAACTCACCGGCGTGATCGTGCCGCGCAAGACGGTCGGCGAAGCCCGCCGCCTGC
TCGATGGCCTTGATGAAGACGTCGAGATCGAAGTCTCCGACACCAAGATCATCCTGCGCGCCGGCCGCGCGGTGCTGACC
TCCAAGCTGATCGATGGCTCCTTCCCCGATTATGCCCGCGTCATCCCCAAAGGGAACGACAAGAAAATGACCGTCGACAA
CAAAGCCTTCGAGGCTGCGGTTGACCGCGTCTCGACGGTCTCGGCAGAGCGCTCGCGCTCGGTGAAGCTCTCCGTTTCCA
ATGGCCGCCTCACCCTGGCGGTGAACCATGCCGAGACCGGCGCCGGCAATGAAGAGATCGAAGCCGAGTATGAGTCCGAG
CCGATGGAAATCGGCTTCAACGCCAAATACCTGCTCGACATCGCCGGCCAGATTGAATCCTCCGAAGTGGAGTTCATGTT
CAATGACCCCGCCTCCCCCGCGCTCGTGCTCGATCCGTCAGACGATTCCGCGCGGTTTGTCGTGATGCCGCTGCGGGTGT
AA

Upstream 100 bases:

>100_bases
GGGCTGATTCCCGGCTTGCCTTGCGGAACAGACTCCAGACACAAACCGGCGATGACGACGAAGCGGGGCCTGGCCGGCCC
CAGGATGAACGGATGAGACG

Downstream 100 bases:

>100_bases
GCCGTGCAGACGGGCCGCCGCATCTTCATCGGCGGCGCGCTCGCCACAGCTGCGCTGGCCGGATTGCCCGGCCATGCAGA
AACCAGGGAGACGGGCATGT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKLTIERGDLLDALSHVQNIVERRTTIPILSNVLLEATQGALKLTATDLDIEAVDSALANVSRPGSVTAPAGTLFDVVRK
LPSGAEVELELNPESQRLTIAAGRSLFELPTLPAADFQTMTPDEGAVSFKIAAKDFARLIDKTRFAISTEETRYYLNGIY
LHAAKADDGSAVLRTVATDGHRLALAELKAPKGAEKLTGVIVPRKTVGEARRLLDGLDEDVEIEVSDTKIILRAGRAVLT
SKLIDGSFPDYARVIPKGNDKKMTVDNKAFEAAVDRVSTVSAERSRSVKLSVSNGRLTLAVNHAETGAGNEEIEAEYESE
PMEIGFNAKYLLDIAGQIESSEVEFMFNDPASPALVLDPSDDSARFVVMPLRV

Sequences:

>Translated_373_residues
MKLTIERGDLLDALSHVQNIVERRTTIPILSNVLLEATQGALKLTATDLDIEAVDSALANVSRPGSVTAPAGTLFDVVRK
LPSGAEVELELNPESQRLTIAAGRSLFELPTLPAADFQTMTPDEGAVSFKIAAKDFARLIDKTRFAISTEETRYYLNGIY
LHAAKADDGSAVLRTVATDGHRLALAELKAPKGAEKLTGVIVPRKTVGEARRLLDGLDEDVEIEVSDTKIILRAGRAVLT
SKLIDGSFPDYARVIPKGNDKKMTVDNKAFEAAVDRVSTVSAERSRSVKLSVSNGRLTLAVNHAETGAGNEEIEAEYESE
PMEIGFNAKYLLDIAGQIESSEVEFMFNDPASPALVLDPSDDSARFVVMPLRV
>Mature_373_residues
MKLTIERGDLLDALSHVQNIVERRTTIPILSNVLLEATQGALKLTATDLDIEAVDSALANVSRPGSVTAPAGTLFDVVRK
LPSGAEVELELNPESQRLTIAAGRSLFELPTLPAADFQTMTPDEGAVSFKIAAKDFARLIDKTRFAISTEETRYYLNGIY
LHAAKADDGSAVLRTVATDGHRLALAELKAPKGAEKLTGVIVPRKTVGEARRLLDGLDEDVEIEVSDTKIILRAGRAVLT
SKLIDGSFPDYARVIPKGNDKKMTVDNKAFEAAVDRVSTVSAERSRSVKLSVSNGRLTLAVNHAETGAGNEEIEAEYESE
PMEIGFNAKYLLDIAGQIESSEVEFMFNDPASPALVLDPSDDSARFVVMPLRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=39.7333333333333, Blast_Score=267, Evalue=8e-73,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40275; Mature: 40275

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTIERGDLLDALSHVQNIVERRTTIPILSNVLLEATQGALKLTATDLDIEAVDSALAN
CEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
VSRPGSVTAPAGTLFDVVRKLPSGAEVELELNPESQRLTIAAGRSLFELPTLPAADFQTM
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCC
TPDEGAVSFKIAAKDFARLIDKTRFAISTEETRYYLNGIYLHAAKADDGSAVLRTVATDG
CCCCCCEEEEEEHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCHHHHEEECCCC
HRLALAELKAPKGAEKLTGVIVPRKTVGEARRLLDGLDEDVEIEVSDTKIILRAGRAVLT
CEEEEEECCCCCCHHHHEEEEECCHHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHHH
SKLIDGSFPDYARVIPKGNDKKMTVDNKAFEAAVDRVSTVSAERSRSVKLSVSNGRLTLA
HHHHCCCCCCHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEE
VNHAETGAGNEEIEAEYESEPMEIGFNAKYLLDIAGQIESSEVEFMFNDPASPALVLDPS
EECCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCEEEEECCCCCCEEEECCC
DDSARFVVMPLRV
CCCCEEEEEEECC
>Mature Secondary Structure
MKLTIERGDLLDALSHVQNIVERRTTIPILSNVLLEATQGALKLTATDLDIEAVDSALAN
CEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHH
VSRPGSVTAPAGTLFDVVRKLPSGAEVELELNPESQRLTIAAGRSLFELPTLPAADFQTM
CCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCEEEEEECCCEEECCCCCCCCCCCC
TPDEGAVSFKIAAKDFARLIDKTRFAISTEETRYYLNGIYLHAAKADDGSAVLRTVATDG
CCCCCCEEEEEEHHHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCHHHHEEECCCC
HRLALAELKAPKGAEKLTGVIVPRKTVGEARRLLDGLDEDVEIEVSDTKIILRAGRAVLT
CEEEEEECCCCCCHHHHEEEEECCHHHHHHHHHHHCCCCCEEEEECCCEEEEECCHHHHH
SKLIDGSFPDYARVIPKGNDKKMTVDNKAFEAAVDRVSTVSAERSRSVKLSVSNGRLTLA
HHHHCCCCCCHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEE
VNHAETGAGNEEIEAEYESEPMEIGFNAKYLLDIAGQIESSEVEFMFNDPASPALVLDPS
EECCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCEEEEECCCCCCEEEECCC
DDSARFVVMPLRV
CCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]