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The map label for this gene is selU [H]

Identifier: 114799852

GI number: 114799852

Start: 2621405

End: 2622487

Strand: Reverse

Name: selU [H]

Synonym: HNE_2491

Alternate gene names: 114799852

Gene position: 2622487-2621405 (Counterclockwise)

Preceding gene: 114800394

Following gene: 114797888

Centisome position: 70.78

GC content: 63.71

Gene sequence:

>1083_bases
ATGGTCTATGTTCTGAAGAACCTTCGCGATCTGGCCGACACTTCGTTTGACGAGATTATCGACGTGCGTTCGCCCGGCGA
GTTTCAGGATGATCATGTGCCCGGCGCCATCAACCTGCCCGTTCTGGACGATGAGGAACGTGCTCTTGTGGGGACGATCT
ACAAGCAGGAGAGCGCCTTTCGTGCCCGCAAGATCGGCGGCGCGCTGGTGGCAAAGCGCGCGGGCCTGAACATCGAGCGC
CACCTCCTCGACAAGCCAGGCAGCTACAGGCCGCTGGTCTATTGTTGGCGCGGGGGCATGCGCTCGGGCGCATTTGCGAC
GATCCTGAAGCAGATCGGGTGGCGGGCCGAAATACTGGAAGGCGGTTACAAGTCTTATCGCCGCGCGGTCGTGGATTTGC
TCTACGCGCCCGGCAGTCCGGCCGAGGTGCTGGCCCGGCGTATCATCGTTGTGGACGGCAATACGGGGACGGCCAAGACC
GACCTGCTTCACCGCATGGCGGCGCGCGGCGCCCAGGTGATGGATCTGGAAGGTCTGGCGGGGCACCGCGGATCGCTGTT
TGGCGGACTGGACGCCTGCCAGCCGTCTCAGAAAACTTTCGACAGCGCGATTGCCCGGCAGCTTGTGATGTTTGATCCGG
CACGGCCGATTTTTGTCGAGGCGGAGTCCAGCCGGATTGGCCGGCTAGCCCTGCCGAATGTCCTCTGGTCTCTGATGCGT
GCAGCGCCCAAGGTCCGGCTGGAGGCCTCGCTCTCAGAGCGCGGAGCTTATCTCGCCCTGGCCTATGCCAATATTGCGTC
TGACCGCGACCGGCTTTCCCGGACGCTGGACCTCCTTAAACCCTACCATGCCGCAGACCGCCTGAAGACCTGGCATGCGC
TGGCCGCGGAGGGACGAATGGAGGACCTTGCCCGTGAACTTGCGGCGCATCACTATGACCCGAAATACGCCCGGCAGCGC
GCCCGCGACCTGCGGCCGGAACTCGGTTGCGTGCGGCTGCAGGGGTTTGGTCCTGATGCGCTGGATCGCGCCGCGCAGGA
AGTTCTGACCCTTGCTGGCGACGCTCTCACTGGTATCAGTTGA

Upstream 100 bases:

>100_bases
ATGGAAATACAAGAAAGGGCCAGGTTGAAATCGTTACCTGACACCTCCCTCGCCGGTAATACGGCTCTTGTCTGGGACGG
TAGAGTAATTAGGCAGGCGT

Downstream 100 bases:

>100_bases
CAAGGATGCGGGGCGGGCCCTCTTCAATTCGGCCGATCACAAAGGCTGGCTCTCCCTCCGCCTGAAACGCCGATAGCAAG
CCGGTGACACTTTGGGACGG

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MVYVLKNLRDLADTSFDEIIDVRSPGEFQDDHVPGAINLPVLDDEERALVGTIYKQESAFRARKIGGALVAKRAGLNIER
HLLDKPGSYRPLVYCWRGGMRSGAFATILKQIGWRAEILEGGYKSYRRAVVDLLYAPGSPAEVLARRIIVVDGNTGTAKT
DLLHRMAARGAQVMDLEGLAGHRGSLFGGLDACQPSQKTFDSAIARQLVMFDPARPIFVEAESSRIGRLALPNVLWSLMR
AAPKVRLEASLSERGAYLALAYANIASDRDRLSRTLDLLKPYHAADRLKTWHALAAEGRMEDLARELAAHHYDPKYARQR
ARDLRPELGCVRLQGFGPDALDRAAQEVLTLAGDALTGIS

Sequences:

>Translated_360_residues
MVYVLKNLRDLADTSFDEIIDVRSPGEFQDDHVPGAINLPVLDDEERALVGTIYKQESAFRARKIGGALVAKRAGLNIER
HLLDKPGSYRPLVYCWRGGMRSGAFATILKQIGWRAEILEGGYKSYRRAVVDLLYAPGSPAEVLARRIIVVDGNTGTAKT
DLLHRMAARGAQVMDLEGLAGHRGSLFGGLDACQPSQKTFDSAIARQLVMFDPARPIFVEAESSRIGRLALPNVLWSLMR
AAPKVRLEASLSERGAYLALAYANIASDRDRLSRTLDLLKPYHAADRLKTWHALAAEGRMEDLARELAAHHYDPKYARQR
ARDLRPELGCVRLQGFGPDALDRAAQEVLTLAGDALTGIS
>Mature_360_residues
MVYVLKNLRDLADTSFDEIIDVRSPGEFQDDHVPGAINLPVLDDEERALVGTIYKQESAFRARKIGGALVAKRAGLNIER
HLLDKPGSYRPLVYCWRGGMRSGAFATILKQIGWRAEILEGGYKSYRRAVVDLLYAPGSPAEVLARRIIVVDGNTGTAKT
DLLHRMAARGAQVMDLEGLAGHRGSLFGGLDACQPSQKTFDSAIARQLVMFDPARPIFVEAESSRIGRLALPNVLWSLMR
AAPKVRLEASLSERGAYLALAYANIASDRDRLSRTLDLLKPYHAADRLKTWHALAAEGRMEDLARELAAHHYDPKYARQR
ARDLRPELGCVRLQGFGPDALDRAAQEVLTLAGDALTGIS

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=239, Percent_Identity=35.9832635983264, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 39654; Mature: 39654

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYVLKNLRDLADTSFDEIIDVRSPGEFQDDHVPGAINLPVLDDEERALVGTIYKQESAF
CEEHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCEEECCEECCCCCHHHHHHHHHHHHH
RARKIGGALVAKRAGLNIERHLLDKPGSYRPLVYCWRGGMRSGAFATILKQIGWRAEILE
HHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCHHHHHH
GGYKSYRRAVVDLLYAPGSPAEVLARRIIVVDGNTGTAKTDLLHRMAARGAQVMDLEGLA
HHHHHHHHHHHHHHCCCCCCHHHHHCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCC
GHRGSLFGGLDACQPSQKTFDSAIARQLVMFDPARPIFVEAESSRIGRLALPNVLWSLMR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
AAPKVRLEASLSERGAYLALAYANIASDRDRLSRTLDLLKPYHAADRLKTWHALAAEGRM
HCCCEEEEECHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
EDLARELAAHHYDPKYARQRARDLRPELGCVRLQGFGPDALDRAAQEVLTLAGDALTGIS
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MVYVLKNLRDLADTSFDEIIDVRSPGEFQDDHVPGAINLPVLDDEERALVGTIYKQESAF
CEEHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCEEECCEECCCCCHHHHHHHHHHHHH
RARKIGGALVAKRAGLNIERHLLDKPGSYRPLVYCWRGGMRSGAFATILKQIGWRAEILE
HHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHCCHHHHHH
GGYKSYRRAVVDLLYAPGSPAEVLARRIIVVDGNTGTAKTDLLHRMAARGAQVMDLEGLA
HHHHHHHHHHHHHHCCCCCCHHHHHCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCC
GHRGSLFGGLDACQPSQKTFDSAIARQLVMFDPARPIFVEAESSRIGRLALPNVLWSLMR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
AAPKVRLEASLSERGAYLALAYANIASDRDRLSRTLDLLKPYHAADRLKTWHALAAEGRM
HCCCEEEEECHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
EDLARELAAHHYDPKYARQRARDLRPELGCVRLQGFGPDALDRAAQEVLTLAGDALTGIS
HHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]