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The map label for this gene is rluF [H]

Identifier: 114799825

GI number: 114799825

Start: 1902097

End: 1903215

Strand: Reverse

Name: rluF [H]

Synonym: HNE_1844

Alternate gene names: 114799825

Gene position: 1903215-1902097 (Counterclockwise)

Preceding gene: 114797077

Following gene: 114799081

Centisome position: 51.37

GC content: 63.63

Gene sequence:

>1119_bases
GTGGCCTGGACCAAGACCTATACCGGCGATGAGCCGTTACGCATCAATAAATGGCTGGCTCAGGAAGGCGTCTGTTCGCG
CCGGGAAGCCGATGCGCTGATTGAAAAAGGCCTTATACAACTGGATGGTAAGCCGGTTACGGTTGCCGGGGAACGCGTCA
CCAAGGGGCAGACGCTGAGCCTTACGGATGCCGCGTCGCGGCGTCTGGATAACCAGCTGTCAGTGATCCTCAACAAGCCT
GTGGGCTATGTTTCGGGGACTCCTGAGCCGGGGGAAATTCCGGCGATCCGGCTGATTTCGGAGGAGAACCTGTCGGGCAG
CGCGCACGCTATCCCGCAGCGTTCGAACAAGATGGCGCCGCTCGGACGGCTCGACAAGGATTCGCATGGACTGCTGATTC
TGTCGGAGGATGGTGTGCTCGCCAAGGCGATCATTGGCCCGCTGAGCGACATGGACAAGGAATACCTTGTCAAAGTGAGA
GGTGACATCACCACCGCCAAGGTGGCGCTGCTACGGCATGGGTTGGAGCTGGACGGGCGGCAGCTGCGCCCGGCGGAAGT
GACCCAGGAACGCGGACAGGTTTTGCGCTTCGTTCTGAAGGAAGGTCGCAACCGGCAGATCCGGCGGATGTGTGAGCTGG
TTGAGCTTAGGGTGTTGGATTTGCAGAGAATTCGGGTTGGTCCGATCCTGTTGGCGGGTCTACCTCAGGGCAAATGGCGG
CCGCTGGGTGCCAAGGAGCGGGCGGCGTTGCTGGCCGCTGGGGCCGGCGAAGAAGTGCGTTCGAGCCCCGCCCGGCGCGA
GGATCGCCTGAGGACAAGTGATGGCCCGCGACGCGAGCGATCTGAGACAGGGCGCGACCAACCCCGGACAGATCGGGACA
GATCGGGACAGTTTGGGACAAAGCCCGGACAGGGGCGGCCAGATAAGCCCCGCACCGGACCCAAACCGGACCGTGCCGGC
GCGAAACCCGCGCGGCCGGGAGCCAAGCCTGCACGTCCTGGCGCAAAGCCCACTGCCGCGCCGGCGCCTGAGGGCCCCAG
CAACCGTCCTTCGGTGCTCAAGGGCGACAAGTTCAAGCGGACGAAGTTGCCTCCCCGGGAACGCAAGCCGATCAAGTGA

Upstream 100 bases:

>100_bases
GGAGGGCAGCAAGGCGCTGTTCGGCGTCTAGCAGCGGGCTGTTGCTGGCCGGGGCGGTGGCCGGTAAGAGGGGCGTTGAT
CCCCGAGGAGCGAACTGGCT

Downstream 100 bases:

>100_bases
TCTTTCCCCCGCGTGACGGCTCGACGGGGGAGCCTGCCGCCGTATCGTGAGTCCCATGGATGGATTTCTCTCAGAACCGA
CCCAGTACAAGGGTCTGACT

Product: ribosomal large subunit pseudouridine synthase F

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MAWTKTYTGDEPLRINKWLAQEGVCSRREADALIEKGLIQLDGKPVTVAGERVTKGQTLSLTDAASRRLDNQLSVILNKP
VGYVSGTPEPGEIPAIRLISEENLSGSAHAIPQRSNKMAPLGRLDKDSHGLLILSEDGVLAKAIIGPLSDMDKEYLVKVR
GDITTAKVALLRHGLELDGRQLRPAEVTQERGQVLRFVLKEGRNRQIRRMCELVELRVLDLQRIRVGPILLAGLPQGKWR
PLGAKERAALLAAGAGEEVRSSPARREDRLRTSDGPRRERSETGRDQPRTDRDRSGQFGTKPGQGRPDKPRTGPKPDRAG
AKPARPGAKPARPGAKPTAAPAPEGPSNRPSVLKGDKFKRTKLPPRERKPIK

Sequences:

>Translated_372_residues
MAWTKTYTGDEPLRINKWLAQEGVCSRREADALIEKGLIQLDGKPVTVAGERVTKGQTLSLTDAASRRLDNQLSVILNKP
VGYVSGTPEPGEIPAIRLISEENLSGSAHAIPQRSNKMAPLGRLDKDSHGLLILSEDGVLAKAIIGPLSDMDKEYLVKVR
GDITTAKVALLRHGLELDGRQLRPAEVTQERGQVLRFVLKEGRNRQIRRMCELVELRVLDLQRIRVGPILLAGLPQGKWR
PLGAKERAALLAAGAGEEVRSSPARREDRLRTSDGPRRERSETGRDQPRTDRDRSGQFGTKPGQGRPDKPRTGPKPDRAG
AKPARPGAKPARPGAKPTAAPAPEGPSNRPSVLKGDKFKRTKLPPRERKPIK
>Mature_371_residues
AWTKTYTGDEPLRINKWLAQEGVCSRREADALIEKGLIQLDGKPVTVAGERVTKGQTLSLTDAASRRLDNQLSVILNKPV
GYVSGTPEPGEIPAIRLISEENLSGSAHAIPQRSNKMAPLGRLDKDSHGLLILSEDGVLAKAIIGPLSDMDKEYLVKVRG
DITTAKVALLRHGLELDGRQLRPAEVTQERGQVLRFVLKEGRNRQIRRMCELVELRVLDLQRIRVGPILLAGLPQGKWRP
LGAKERAALLAAGAGEEVRSSPARREDRLRTSDGPRRERSETGRDQPRTDRDRSGQFGTKPGQGRPDKPRTGPKPDRAGA
KPARPGAKPARPGAKPTAAPAPEGPSNRPSVLKGDKFKRTKLPPRERKPIK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=244, Percent_Identity=36.8852459016393, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=31.7073170731707, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788510, Length=242, Percent_Identity=27.6859504132231, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI87081838, Length=138, Percent_Identity=36.9565217391304, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 40733; Mature: 40602

Theoretical pI: Translated: 11.13; Mature: 11.13

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWTKTYTGDEPLRINKWLAQEGVCSRREADALIEKGLIQLDGKPVTVAGERVTKGQTLS
CCCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHCCCEEECCEEEEEECCEECCCCEEE
LTDAASRRLDNQLSVILNKPVGYVSGTPEPGEIPAIRLISEENLSGSAHAIPQRSNKMAP
EHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
LGRLDKDSHGLLILSEDGVLAKAIIGPLSDMDKEYLVKVRGDITTAKVALLRHGLELDGR
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHEEEECCCHHHHHHHHHHHCCCCCCC
QLRPAEVTQERGQVLRFVLKEGRNRQIRRMCELVELRVLDLQRIRVGPILLAGLPQGKWR
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECEEEEECCCCCCCC
PLGAKERAALLAAGAGEEVRSSPARREDRLRTSDGPRRERSETGRDQPRTDRDRSGQFGT
CCCCHHHHEEEECCCCHHHHCCCHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
KPGQGRPDKPRTGPKPDRAGAKPARPGAKPARPGAKPTAAPAPEGPSNRPSVLKGDKFKR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TKLPPRERKPIK
CCCCCCCCCCCC
>Mature Secondary Structure 
AWTKTYTGDEPLRINKWLAQEGVCSRREADALIEKGLIQLDGKPVTVAGERVTKGQTLS
CCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHCCCEEECCEEEEEECCEECCCCEEE
LTDAASRRLDNQLSVILNKPVGYVSGTPEPGEIPAIRLISEENLSGSAHAIPQRSNKMAP
EHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
LGRLDKDSHGLLILSEDGVLAKAIIGPLSDMDKEYLVKVRGDITTAKVALLRHGLELDGR
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHEEEECCCHHHHHHHHHHHCCCCCCC
QLRPAEVTQERGQVLRFVLKEGRNRQIRRMCELVELRVLDLQRIRVGPILLAGLPQGKWR
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECEEEEECCCCCCCC
PLGAKERAALLAAGAGEEVRSSPARREDRLRTSDGPRRERSETGRDQPRTDRDRSGQFGT
CCCCHHHHEEEECCCCHHHHCCCHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCC
KPGQGRPDKPRTGPKPDRAGAKPARPGAKPARPGAKPTAAPAPEGPSNRPSVLKGDKFKR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TKLPPRERKPIK
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]