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The map label for this gene is phoA [H]

Identifier: 114799748

GI number: 114799748

Start: 3616953

End: 3618542

Strand: Direct

Name: phoA [H]

Synonym: HNE_3483

Alternate gene names: 114799748

Gene position: 3616953-3618542 (Clockwise)

Preceding gene: 114797206

Following gene: 114798593

Centisome position: 97.62

GC content: 64.47

Gene sequence:

>1590_bases
ATGCGCCTGGCTGTTGCTGCCCTTGTTTCCACGCTAGCCCTTGCCGCCGCCTGCGCCGCAGCCGTTGCCACAAGCGCAGC
GCCCTCGACAAGCACAGCGCCACGCCACGAGCCTATCCTCCCGGTTCAAGCCGAAGATACCTACTATAAATCTGCCGCTG
AAGCGGTCGACATTCGCATCGCGGAACGCGGCATCAAACCGGCCAAGAACGTCATCCTGTTCGTCGGCGACGGGATGGGC
GTTTCCACCATCACCGCTTCGCGCATCTATGCCGGCCAGTCTGCTGGCGTCGACGGCGAGAGCTTCCGCCTCGCCATGGA
ATCCCTGCCCTGGTCAGCCCTCTCCAAGACCTACAGCCACGACTATCAGGTCTCCGACTCGGCGGCGACCGCCACAGCCA
TGACCGCCGGCCTCAAGACCAAATCGGGCTTCCTCGGTGTCTCCAGCGCTGCTAATTTCGGCAACTGCGCCAGCGCTCAG
GGAACCGAAGCCGATACGCTGTTCGAGATCGCCCAGCGCGCGGGCCTCGCCACCGGCGTCATTTCTACCGCGCGCATCAC
CCATGCCACGCCGGGCGCCACCTATGCCAAGGTTCCCCACCGCAACTGGGAGGCCGACGCTGACATGCGCGGGGCCTCAT
CCGACACGTGCAAGGACATTGCCCGCCAGCTGATCGAAGGCCCCTCCAGCGACTTCGACGTGATCCTTGGCGGCGGCCGC
GCGAAATTCCTGCCTGCCGAAACGCCTGACCCGGAATATCCCGATCAGACCGGCGAGCGCGCTGATGGCCGCAACCTGAT
GGAAGAATGGGCCGCCAAGGACGGGACGCGCAAGACCGTGTTCGATCGCGCAGGCTTTGCCGCGATCGACTTTGCCTCTG
ATGTGAAGGTCTTCGGCCTCTTCGAACCCTCCCACATGCAATACGAACTCGACCGGGAGGCCGACACCGCGGGCGAGCCT
TCACTGGAAGAGATGACCCGCGCCGCCATCACCCGCCTCTCGCGCAATGAAGATGGCTTTGTACTGATGGTGGAAGGTGG
CCGGATCGACCATGCCCACCACGCTGGCAACGCCATCCGCGCGCTGGAAGATACCCTCGCCTTTGACGCGGCCATCGCGG
CGGCGCTGGAAATGACGAACGCGGAAGACACGCTGATCATCGTCACCGCCGACCATTCCCATAGCCTGACGATCAATGGT
TATCCCCAGCGCGGTAATCCGATCCTCGGCCTTGTCACCGCCGGCGCCTCATCCGAAGGCGGATCACTCGGCGCCGACGG
CCTGCCCTACACCACGCTGTCTTACGCCAACGGCCCCGGTGCCTGCCGCGAAACCGACAAGGACGCCGAGGGTAAGCCGG
AATATGACTGCAAGCGCTACGACCTGACAGGGATCGACACCGGCGCGCCGGATTTCCGCCAGCAATCCCTCGTTCCGCTC
TATTCGGAAACGCATGGCGGCGAAGACGTTGCCGCCTTCGCCTCCGGGCCGGGCGCCAACCTCATCTCCGGCGTGATCGA
GCAGAACGAGATCTTCCACGTCATGGGCCGGGCCGTTGGTCTGATCCCTGCCCCAGCCGCCGAAGAATGA

Upstream 100 bases:

>100_bases
GCTCAGCGCCAAACCGTCCCGCCAGCGGGATAAATTCTCGCAACTGTCACAGGATTGAGTTAAGCGCGGGAGACGTAATC
ATCGCCTGAAGGAGGCTCTC

Downstream 100 bases:

>100_bases
GCTGACAGTTATCGGCCAGCCGGGCGCAAAACCCCGCTGGCCATGACGGGCACGAAATAGGCAATCGCCAGCGCTGTGTT
CGGCACAATGTTCAGCGCAA

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MRLAVAALVSTLALAAACAAAVATSAAPSTSTAPRHEPILPVQAEDTYYKSAAEAVDIRIAERGIKPAKNVILFVGDGMG
VSTITASRIYAGQSAGVDGESFRLAMESLPWSALSKTYSHDYQVSDSAATATAMTAGLKTKSGFLGVSSAANFGNCASAQ
GTEADTLFEIAQRAGLATGVISTARITHATPGATYAKVPHRNWEADADMRGASSDTCKDIARQLIEGPSSDFDVILGGGR
AKFLPAETPDPEYPDQTGERADGRNLMEEWAAKDGTRKTVFDRAGFAAIDFASDVKVFGLFEPSHMQYELDREADTAGEP
SLEEMTRAAITRLSRNEDGFVLMVEGGRIDHAHHAGNAIRALEDTLAFDAAIAAALEMTNAEDTLIIVTADHSHSLTING
YPQRGNPILGLVTAGASSEGGSLGADGLPYTTLSYANGPGACRETDKDAEGKPEYDCKRYDLTGIDTGAPDFRQQSLVPL
YSETHGGEDVAAFASGPGANLISGVIEQNEIFHVMGRAVGLIPAPAAEE

Sequences:

>Translated_529_residues
MRLAVAALVSTLALAAACAAAVATSAAPSTSTAPRHEPILPVQAEDTYYKSAAEAVDIRIAERGIKPAKNVILFVGDGMG
VSTITASRIYAGQSAGVDGESFRLAMESLPWSALSKTYSHDYQVSDSAATATAMTAGLKTKSGFLGVSSAANFGNCASAQ
GTEADTLFEIAQRAGLATGVISTARITHATPGATYAKVPHRNWEADADMRGASSDTCKDIARQLIEGPSSDFDVILGGGR
AKFLPAETPDPEYPDQTGERADGRNLMEEWAAKDGTRKTVFDRAGFAAIDFASDVKVFGLFEPSHMQYELDREADTAGEP
SLEEMTRAAITRLSRNEDGFVLMVEGGRIDHAHHAGNAIRALEDTLAFDAAIAAALEMTNAEDTLIIVTADHSHSLTING
YPQRGNPILGLVTAGASSEGGSLGADGLPYTTLSYANGPGACRETDKDAEGKPEYDCKRYDLTGIDTGAPDFRQQSLVPL
YSETHGGEDVAAFASGPGANLISGVIEQNEIFHVMGRAVGLIPAPAAEE
>Mature_529_residues
MRLAVAALVSTLALAAACAAAVATSAAPSTSTAPRHEPILPVQAEDTYYKSAAEAVDIRIAERGIKPAKNVILFVGDGMG
VSTITASRIYAGQSAGVDGESFRLAMESLPWSALSKTYSHDYQVSDSAATATAMTAGLKTKSGFLGVSSAANFGNCASAQ
GTEADTLFEIAQRAGLATGVISTARITHATPGATYAKVPHRNWEADADMRGASSDTCKDIARQLIEGPSSDFDVILGGGR
AKFLPAETPDPEYPDQTGERADGRNLMEEWAAKDGTRKTVFDRAGFAAIDFASDVKVFGLFEPSHMQYELDREADTAGEP
SLEEMTRAAITRLSRNEDGFVLMVEGGRIDHAHHAGNAIRALEDTLAFDAAIAAALEMTNAEDTLIIVTADHSHSLTING
YPQRGNPILGLVTAGASSEGGSLGADGLPYTTLSYANGPGACRETDKDAEGKPEYDCKRYDLTGIDTGAPDFRQQSLVPL
YSETHGGEDVAAFASGPGANLISGVIEQNEIFHVMGRAVGLIPAPAAEE

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=502, Percent_Identity=46.8127490039841, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI94721246, Length=502, Percent_Identity=46.6135458167331, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI157266292, Length=502, Percent_Identity=45.6175298804781, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI116734717, Length=512, Percent_Identity=41.6015625, Blast_Score=352, Evalue=6e-97,
Organism=Homo sapiens, GI294660770, Length=453, Percent_Identity=43.4878587196468, Blast_Score=341, Evalue=1e-93,
Organism=Homo sapiens, GI294660772, Length=431, Percent_Identity=41.5313225058005, Blast_Score=296, Evalue=4e-80,
Organism=Escherichia coli, GI48994877, Length=493, Percent_Identity=31.6430020283976, Blast_Score=154, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6320689, Length=348, Percent_Identity=34.1954022988506, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI85725200, Length=496, Percent_Identity=42.5403225806452, Blast_Score=348, Evalue=7e-96,
Organism=Drosophila melanogaster, GI85815833, Length=496, Percent_Identity=42.5403225806452, Blast_Score=348, Evalue=7e-96,
Organism=Drosophila melanogaster, GI19921912, Length=469, Percent_Identity=43.7100213219616, Blast_Score=337, Evalue=9e-93,
Organism=Drosophila melanogaster, GI21355149, Length=468, Percent_Identity=42.9487179487179, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI21358067, Length=462, Percent_Identity=42.8571428571429, Blast_Score=337, Evalue=2e-92,
Organism=Drosophila melanogaster, GI21355151, Length=502, Percent_Identity=41.0358565737052, Blast_Score=333, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24651554, Length=477, Percent_Identity=42.7672955974843, Blast_Score=324, Evalue=9e-89,
Organism=Drosophila melanogaster, GI24657835, Length=478, Percent_Identity=39.5397489539749, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI21355981, Length=542, Percent_Identity=38.1918819188192, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24657842, Length=479, Percent_Identity=38.4133611691023, Blast_Score=311, Evalue=7e-85,
Organism=Drosophila melanogaster, GI24657827, Length=481, Percent_Identity=39.5010395010395, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24651556, Length=464, Percent_Identity=42.2413793103448, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24585213, Length=533, Percent_Identity=36.5853658536585, Blast_Score=288, Evalue=8e-78,
Organism=Drosophila melanogaster, GI18859923, Length=527, Percent_Identity=36.4326375711575, Blast_Score=285, Evalue=7e-77,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 55385; Mature: 55385

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAVAALVSTLALAAACAAAVATSAAPSTSTAPRHEPILPVQAEDTYYKSAAEAVDIRI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEEE
AERGIKPAKNVILFVGDGMGVSTITASRIYAGQSAGVDGESFRLAMESLPWSALSKTYSH
HHCCCCCCCCEEEEEECCCCCHHEEHHHEECCCCCCCCCHHHHHHHHHCCHHHHHHHHCC
DYQVSDSAATATAMTAGLKTKSGFLGVSSAANFGNCASAQGTEADTLFEIAQRAGLATGV
CEEECCCHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHH
ISTARITHATPGATYAKVPHRNWEADADMRGASSDTCKDIARQLIEGPSSDFDVILGGGR
HHHHEEEECCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCC
AKFLPAETPDPEYPDQTGERADGRNLMEEWAAKDGTRKTVFDRAGFAAIDFASDVKVFGL
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCEEEEE
FEPSHMQYELDREADTAGEPSLEEMTRAAITRLSRNEDGFVLMVEGGRIDHAHHAGNAIR
ECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCHHHH
ALEDTLAFDAAIAAALEMTNAEDTLIIVTADHSHSLTINGYPQRGNPILGLVTAGASSEG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCCEEEEEEECCCCCC
GSLGADGLPYTTLSYANGPGACRETDKDAEGKPEYDCKRYDLTGIDTGAPDFRQQSLVPL
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHCCCCCH
YSETHGGEDVAAFASGPGANLISGVIEQNEIFHVMGRAVGLIPAPAAEE
HHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRLAVAALVSTLALAAACAAAVATSAAPSTSTAPRHEPILPVQAEDTYYKSAAEAVDIRI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEEE
AERGIKPAKNVILFVGDGMGVSTITASRIYAGQSAGVDGESFRLAMESLPWSALSKTYSH
HHCCCCCCCCEEEEEECCCCCHHEEHHHEECCCCCCCCCHHHHHHHHHCCHHHHHHHHCC
DYQVSDSAATATAMTAGLKTKSGFLGVSSAANFGNCASAQGTEADTLFEIAQRAGLATGV
CEEECCCHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHH
ISTARITHATPGATYAKVPHRNWEADADMRGASSDTCKDIARQLIEGPSSDFDVILGGGR
HHHHEEEECCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCC
AKFLPAETPDPEYPDQTGERADGRNLMEEWAAKDGTRKTVFDRAGFAAIDFASDVKVFGL
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCEEEEE
FEPSHMQYELDREADTAGEPSLEEMTRAAITRLSRNEDGFVLMVEGGRIDHAHHAGNAIR
ECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCHHHH
ALEDTLAFDAAIAAALEMTNAEDTLIIVTADHSHSLTINGYPQRGNPILGLVTAGASSEG
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCCCCEEEEEEECCCCCC
GSLGADGLPYTTLSYANGPGACRETDKDAEGKPEYDCKRYDLTGIDTGAPDFRQQSLVPL
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHCCCCCH
YSETHGGEDVAAFASGPGANLISGVIEQNEIFHVMGRAVGLIPAPAAEE
HHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]