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The map label for this gene is tgt [H]

Identifier: 114799701

GI number: 114799701

Start: 1141651

End: 1142790

Strand: Direct

Name: tgt [H]

Synonym: HNE_1130

Alternate gene names: 114799701

Gene position: 1141651-1142790 (Clockwise)

Preceding gene: 114800442

Following gene: 114799307

Centisome position: 30.81

GC content: 64.56

Gene sequence:

>1140_bases
ATGGCTACGTTCCCGTTTGAAATTTCCGCCCGTGAGGGTAAGGCGCGCACCGGGCGCCTGAAAACCCCGCGCGGCGACAT
CCGCACGCCCGCCTTCATGCCCGTCGGCACCGCCGGCACGGTCAAGGCCCTCTATATGGATCAGGTCCAGGGGGCGGGGG
CCGACATCATCCTCGGCAACACCTACCATCTGATGCTCCGCCCCGGCGCCGAGCGCGTGCAACGCCTCGGCGGCCTGCAC
AAATTCTCCGGCTGGTCCGGCCCGATGCTGACCGACTCCGGCGGCTTCCAGGTCTGGTCGCTCGCCCAGCTTCGGAAGAT
GGACGCAGACGGCGTCACCTTCCAGAGCCATATCGACGGCTCCACCCACCGCCTCACCCCCGCCCGCTCCATCGAAATTC
AGGCAGACCTTCTGGGCGCTGACATCTCCATGCAGCTTGATGAGTGCACAAACTTTCCGTGTACGCATGAAGAGGCCGAG
CGCAGCCTCGCCCTCTCCCTCAAATGGGGGGAACGCAGCCTCGACGCCTTCGGCGCCCGAGACGCACAAACTCTCTTTGG
AATCATTCAAGGCTCAAACTTCCCGGACCTGCGCGAGCGCTCCGCCAAGGGCGTCACTGCCCAAGGCTTCGGCGGCATCG
CGCTCGGCGGTCTCGCCGTCGGTGAAGGCCATGACCAGATGTGCGCCACCATCGACATGACGATGCCCCATCTTCCCGAT
CACATGCCCCGCTATGTCATGGGCGTCGGCAAACCCATCGACCTCGTCGAAAGCGTTGCCCGCGGGATCGACATGTTCGA
CTGTGTCCTGCCTACCCGCTCGGGCCGCCACGGCCAGGCCTGGACCTGGGACGGCCCCCTCAATCTCAACAACGCGAAGT
TTGCCGAAGACACGAGCCCGCTCGATCCGGTGATCAACTGCCCGGCCAGCCAGGTCTATTCCCGCGCCTACCTGCATCAC
CTGTTCAAGGCCGGCGAATATCTCGGTCCCATGCTCCTCTCCTGGCACAACACCGCCTTCTTCCAGGCCCTGATGGCCGA
GATGCGCGCCGCCATCGCCGAAGGCCGCTTCGAAGCCCTGCGCGCAACGCTCAGCGCCGGCTGGGAAAACACGAAGTCAA
AACCGAAAGCTGAAAGCTGA

Upstream 100 bases:

>100_bases
TGCGCGCCTTCGGCATGCTCATGGCCGCCTGGCAGGTGTTCATGGCGGGGTGATCCGCGCAGCTTGGCTTCCATGGGGCG
CTCCCGTATAGGAGCGCCCC

Downstream 100 bases:

>100_bases
CTTCAATATGGCGTCATCCCGGAAATCGCGAAGCGATTATCCAGAACCCAGAATGCACGGCTCAACAGCTCGCCAGCCTA
CTTCAACTGCTCCAGCGCCT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MATFPFEISAREGKARTGRLKTPRGDIRTPAFMPVGTAGTVKALYMDQVQGAGADIILGNTYHLMLRPGAERVQRLGGLH
KFSGWSGPMLTDSGGFQVWSLAQLRKMDADGVTFQSHIDGSTHRLTPARSIEIQADLLGADISMQLDECTNFPCTHEEAE
RSLALSLKWGERSLDAFGARDAQTLFGIIQGSNFPDLRERSAKGVTAQGFGGIALGGLAVGEGHDQMCATIDMTMPHLPD
HMPRYVMGVGKPIDLVESVARGIDMFDCVLPTRSGRHGQAWTWDGPLNLNNAKFAEDTSPLDPVINCPASQVYSRAYLHH
LFKAGEYLGPMLLSWHNTAFFQALMAEMRAAIAEGRFEALRATLSAGWENTKSKPKAES

Sequences:

>Translated_379_residues
MATFPFEISAREGKARTGRLKTPRGDIRTPAFMPVGTAGTVKALYMDQVQGAGADIILGNTYHLMLRPGAERVQRLGGLH
KFSGWSGPMLTDSGGFQVWSLAQLRKMDADGVTFQSHIDGSTHRLTPARSIEIQADLLGADISMQLDECTNFPCTHEEAE
RSLALSLKWGERSLDAFGARDAQTLFGIIQGSNFPDLRERSAKGVTAQGFGGIALGGLAVGEGHDQMCATIDMTMPHLPD
HMPRYVMGVGKPIDLVESVARGIDMFDCVLPTRSGRHGQAWTWDGPLNLNNAKFAEDTSPLDPVINCPASQVYSRAYLHH
LFKAGEYLGPMLLSWHNTAFFQALMAEMRAAIAEGRFEALRATLSAGWENTKSKPKAES
>Mature_378_residues
ATFPFEISAREGKARTGRLKTPRGDIRTPAFMPVGTAGTVKALYMDQVQGAGADIILGNTYHLMLRPGAERVQRLGGLHK
FSGWSGPMLTDSGGFQVWSLAQLRKMDADGVTFQSHIDGSTHRLTPARSIEIQADLLGADISMQLDECTNFPCTHEEAER
SLALSLKWGERSLDAFGARDAQTLFGIIQGSNFPDLRERSAKGVTAQGFGGIALGGLAVGEGHDQMCATIDMTMPHLPDH
MPRYVMGVGKPIDLVESVARGIDMFDCVLPTRSGRHGQAWTWDGPLNLNNAKFAEDTSPLDPVINCPASQVYSRAYLHHL
FKAGEYLGPMLLSWHNTAFFQALMAEMRAAIAEGRFEALRATLSAGWENTKSKPKAES

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=344, Percent_Identity=43.0232558139535, Blast_Score=279, Evalue=3e-75,
Organism=Escherichia coli, GI1786607, Length=358, Percent_Identity=51.3966480446927, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=42.6553672316384, Blast_Score=264, Evalue=7e-71,
Organism=Drosophila melanogaster, GI19920510, Length=364, Percent_Identity=42.5824175824176, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI21355311, Length=247, Percent_Identity=24.2914979757085, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41250; Mature: 41119

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATFPFEISAREGKARTGRLKTPRGDIRTPAFMPVGTAGTVKALYMDQVQGAGADIILGN
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCEEEECC
TYHLMLRPGAERVQRLGGLHKFSGWSGPMLTDSGGFQVWSLAQLRKMDADGVTFQSHIDG
EEEEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCCEEHHHHHHHHCCCCCCEEECCCCC
STHRLTPARSIEIQADLLGADISMQLDECTNFPCTHEEAERSLALSLKWGERSLDAFGAR
CCEECCCCCEEEEEEEEECCCCEEEHHHHCCCCCCHHHHCCEEEEEEECCCCCHHHHCCC
DAQTLFGIIQGSNFPDLRERSAKGVTAQGFGGIALGGLAVGEGHDQMCATIDMTMPHLPD
CHHHHHHHHCCCCCCHHHHHCCCCCEECCCCCEEECCEEECCCCCCEEEEEECCCCCCCC
HMPRYVMGVGKPIDLVESVARGIDMFDCVLPTRSGRHGQAWTWDGPLNLNNAKFAEDTSP
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCC
LDPVINCPASQVYSRAYLHHLFKAGEYLGPMLLSWHNTAFFQALMAEMRAAIAEGRFEAL
CCHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
RATLSAGWENTKSKPKAES
HHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ATFPFEISAREGKARTGRLKTPRGDIRTPAFMPVGTAGTVKALYMDQVQGAGADIILGN
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCCEEEECC
TYHLMLRPGAERVQRLGGLHKFSGWSGPMLTDSGGFQVWSLAQLRKMDADGVTFQSHIDG
EEEEEECCCHHHHHHHCCCCCCCCCCCCEEECCCCCEEHHHHHHHHCCCCCCEEECCCCC
STHRLTPARSIEIQADLLGADISMQLDECTNFPCTHEEAERSLALSLKWGERSLDAFGAR
CCEECCCCCEEEEEEEEECCCCEEEHHHHCCCCCCHHHHCCEEEEEEECCCCCHHHHCCC
DAQTLFGIIQGSNFPDLRERSAKGVTAQGFGGIALGGLAVGEGHDQMCATIDMTMPHLPD
CHHHHHHHHCCCCCCHHHHHCCCCCEECCCCCEEECCEEECCCCCCEEEEEECCCCCCCC
HMPRYVMGVGKPIDLVESVARGIDMFDCVLPTRSGRHGQAWTWDGPLNLNNAKFAEDTSP
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCC
LDPVINCPASQVYSRAYLHHLFKAGEYLGPMLLSWHNTAFFQALMAEMRAAIAEGRFEAL
CCHHHCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
RATLSAGWENTKSKPKAES
HHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]