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The map label for this gene is proS

Identifier: 114799658

GI number: 114799658

Start: 1805221

End: 1806540

Strand: Direct

Name: proS

Synonym: HNE_1764

Alternate gene names: 114799658

Gene position: 1805221-1806540 (Clockwise)

Preceding gene: 114800295

Following gene: 114798078

Centisome position: 48.72

GC content: 56.36

Gene sequence:

>1320_bases
ATGCGCCTGTCGAAGTTTTTTCTGCCTGTTTCCAAAGAAGCGCCTGCGGATGCCGCGATCGTTTCTCACCAGCTGATGCT
GCGTACCGGTATGATCCGGCAGAATGGCGCAGGCATCTACTCCTGGCTGCCTCTGGGTTATCGCGTACTCAAACGAATTG
AGCAGATTGTACGCGAGGAAATGAACCGGGCGGGCGCCATCGAAATGCTGATGCCGACGCTGCAACAGGCTGATCTCTGG
CGGGAGTCCGGCCGGTATGACGACTATGGCAAAGAGATGTTGCGTATCAAGGATCGCCATGAGCGGGACATGCTGTACGG
CCCGACAAATGAAGAACTGATCACGGATGTCTTCCGGACCTATGTAAAAAGCTACAAGCAGCTACCTCTGAACCTGTATC
ACCAGCAGTGGAAGTTCCGCGACGAGGTGCGGCCCCGTTTTGGTGTCATGCGCGGTCGTGAGTTCCTCATGAAAGACGCC
TATTCGTTTGATCTGACTGAGGAAGACGCCAGAGCGGCGTACCGCAAGATGTTCTGTGCCTATCTGAATGCATTTGACCG
GATGGGCCTCACGGCCATCCCGATGCGTGCAGACACGGGGCCGATCGGCGGCGATCTCAGCCACGAATTCATTATTCTGG
CAGATACTGGCGAGAGCGCCGTCTTCTGTGACAAGGCTCTCCTCGATCTTCCGGCCCCTGGCTTAGACCTCGATTTCGAG
TCAGACCTCACGCCATTCGTGACTGAGCGCACCGGATATTATGCGGCAACCGAAGAGATGCATGATGAGGCCGCATTCAA
TGCGCTGCCGGAAGACCGGCGTGTTTCCGCGCGGGGAATTGAAGTAGGTCACATCTTTTTCTTTGGCACCAAATACTCAA
AGCCGATGAACGCTGTGGTGCAGGGGCCTGATGGTCAGATGGTGCCAGTTCAGATGGGCAGTTATGGCATTGGTGTTTCG
CGGCTACTCGGTGCGATCATCGAGGCATGTCACGACGAGAACGGCATTGTATGGCCGGAGTCGGTTGCCCCCTTCGATGT
TGGCCTGATCAATATGCGTCCCGGCAATGAAGCCTGCGATGCAGCCTGTAACACGCTATACGCTGCGTTGACTGCAGCTG
GCCGTGAAGTGCTCTACGACGAAACCGATGACCGCGCAGGGGCGAAGTTTGCGCGCATGGATCTCATCGGCCTGCCCTGG
CAAACGACCGTCGGGCCGCGCGGCGTTACTGAAGGCAAGGTCGAGGTCAAGAATCGCAAGACGGGCGAGAAGCATGATGT
CAAAATCGAAGATATGCTGGCTCAACTGAAAGGCTCCTGA

Upstream 100 bases:

>100_bases
TTACGGCGGCCGCTGCTGTGGTTGTTCCTCTCCTGCTTGCGGAACAGCCCTAGCTGCGGCTAGTGAGCGTGAAAGAATTC
CGTAAAAGTCTGGATGATTC

Downstream 100 bases:

>100_bases
TGAGCGCGATGCCTTCCGCAACGCCTTTTGGCAAACTGGAGCGAACGCTTGCCTGGAGGTATCTCAGGGCGCGCCGTGAA
CATGGGGGCGCCAGCCTTAT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MRLSKFFLPVSKEAPADAAIVSHQLMLRTGMIRQNGAGIYSWLPLGYRVLKRIEQIVREEMNRAGAIEMLMPTLQQADLW
RESGRYDDYGKEMLRIKDRHERDMLYGPTNEELITDVFRTYVKSYKQLPLNLYHQQWKFRDEVRPRFGVMRGREFLMKDA
YSFDLTEEDARAAYRKMFCAYLNAFDRMGLTAIPMRADTGPIGGDLSHEFIILADTGESAVFCDKALLDLPAPGLDLDFE
SDLTPFVTERTGYYAATEEMHDEAAFNALPEDRRVSARGIEVGHIFFFGTKYSKPMNAVVQGPDGQMVPVQMGSYGIGVS
RLLGAIIEACHDENGIVWPESVAPFDVGLINMRPGNEACDAACNTLYAALTAAGREVLYDETDDRAGAKFARMDLIGLPW
QTTVGPRGVTEGKVEVKNRKTGEKHDVKIEDMLAQLKGS

Sequences:

>Translated_439_residues
MRLSKFFLPVSKEAPADAAIVSHQLMLRTGMIRQNGAGIYSWLPLGYRVLKRIEQIVREEMNRAGAIEMLMPTLQQADLW
RESGRYDDYGKEMLRIKDRHERDMLYGPTNEELITDVFRTYVKSYKQLPLNLYHQQWKFRDEVRPRFGVMRGREFLMKDA
YSFDLTEEDARAAYRKMFCAYLNAFDRMGLTAIPMRADTGPIGGDLSHEFIILADTGESAVFCDKALLDLPAPGLDLDFE
SDLTPFVTERTGYYAATEEMHDEAAFNALPEDRRVSARGIEVGHIFFFGTKYSKPMNAVVQGPDGQMVPVQMGSYGIGVS
RLLGAIIEACHDENGIVWPESVAPFDVGLINMRPGNEACDAACNTLYAALTAAGREVLYDETDDRAGAKFARMDLIGLPW
QTTVGPRGVTEGKVEVKNRKTGEKHDVKIEDMLAQLKGS
>Mature_439_residues
MRLSKFFLPVSKEAPADAAIVSHQLMLRTGMIRQNGAGIYSWLPLGYRVLKRIEQIVREEMNRAGAIEMLMPTLQQADLW
RESGRYDDYGKEMLRIKDRHERDMLYGPTNEELITDVFRTYVKSYKQLPLNLYHQQWKFRDEVRPRFGVMRGREFLMKDA
YSFDLTEEDARAAYRKMFCAYLNAFDRMGLTAIPMRADTGPIGGDLSHEFIILADTGESAVFCDKALLDLPAPGLDLDFE
SDLTPFVTERTGYYAATEEMHDEAAFNALPEDRRVSARGIEVGHIFFFGTKYSKPMNAVVQGPDGQMVPVQMGSYGIGVS
RLLGAIIEACHDENGIVWPESVAPFDVGLINMRPGNEACDAACNTLYAALTAAGREVLYDETDDRAGAKFARMDLIGLPW
QTTVGPRGVTEGKVEVKNRKTGEKHDVKIEDMLAQLKGS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=417, Percent_Identity=35.7314148681055, Blast_Score=248, Evalue=8e-66,
Organism=Escherichia coli, GI1786392, Length=261, Percent_Identity=54.7892720306513, Blast_Score=286, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI115532348, Length=388, Percent_Identity=30.9278350515464, Blast_Score=182, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI193203271, Length=111, Percent_Identity=30.6306306306306, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=300, Percent_Identity=31.3333333333333, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24656200, Length=422, Percent_Identity=29.8578199052133, Blast_Score=207, Evalue=1e-53,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_HYPNA (Q0C1C5)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760468.1
- ProteinModelPortal:   Q0C1C5
- SMR:   Q0C1C5
- STRING:   Q0C1C5
- GeneID:   4288680
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1764
- NMPDR:   fig|228405.5.peg.1698
- TIGR:   HNE_1764
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q0C1C5
- ProtClustDB:   PRK12325
- BioCyc:   HNEP81032:HNE_1764-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 49401; Mature: 49401

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSKFFLPVSKEAPADAAIVSHQLMLRTGMIRQNGAGIYSWLPLGYRVLKRIEQIVREE
CCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIEMLMPTLQQADLWRESGRYDDYGKEMLRIKDRHERDMLYGPTNEELITDVFRT
HHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHH
YVKSYKQLPLNLYHQQWKFRDEVRPRFGVMRGREFLMKDAYSFDLTEEDARAAYRKMFCA
HHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
YLNAFDRMGLTAIPMRADTGPIGGDLSHEFIILADTGESAVFCDKALLDLPAPGLDLDFE
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHCCCCCCCCCCC
SDLTPFVTERTGYYAATEEMHDEAAFNALPEDRRVSARGIEVGHIFFFGTKYSKPMNAVV
CCCCCHHCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEE
QGPDGQMVPVQMGSYGIGVSRLLGAIIEACHDENGIVWPESVAPFDVGLINMRPGNEACD
ECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCHHHH
AACNTLYAALTAAGREVLYDETDDRAGAKFARMDLIGLPWQTTVGPRGVTEGKVEVKNRK
HHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHEEECCCCCCCCCCCCCCCCEEEECCCC
TGEKHDVKIEDMLAQLKGS
CCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRLSKFFLPVSKEAPADAAIVSHQLMLRTGMIRQNGAGIYSWLPLGYRVLKRIEQIVREE
CCCCHHCCCCCCCCCCHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
MNRAGAIEMLMPTLQQADLWRESGRYDDYGKEMLRIKDRHERDMLYGPTNEELITDVFRT
HHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHH
YVKSYKQLPLNLYHQQWKFRDEVRPRFGVMRGREFLMKDAYSFDLTEEDARAAYRKMFCA
HHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
YLNAFDRMGLTAIPMRADTGPIGGDLSHEFIILADTGESAVFCDKALLDLPAPGLDLDFE
HHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEHHHHHHHCCCCCCCCCCC
SDLTPFVTERTGYYAATEEMHDEAAFNALPEDRRVSARGIEVGHIFFFGTKYSKPMNAVV
CCCCCHHCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCEE
QGPDGQMVPVQMGSYGIGVSRLLGAIIEACHDENGIVWPESVAPFDVGLINMRPGNEACD
ECCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCEEEEEECCCCHHHH
AACNTLYAALTAAGREVLYDETDDRAGAKFARMDLIGLPWQTTVGPRGVTEGKVEVKNRK
HHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHEEECCCCCCCCCCCCCCCCEEEECCCC
TGEKHDVKIEDMLAQLKGS
CCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA