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The map label for this gene is nuoB

Identifier: 114799608

GI number: 114799608

Start: 1785840

End: 1786412

Strand: Direct

Name: nuoB

Synonym: HNE_1743

Alternate gene names: 114799608

Gene position: 1785840-1786412 (Clockwise)

Preceding gene: 114798172

Following gene: 114799741

Centisome position: 48.2

GC content: 61.61

Gene sequence:

>573_bases
ATGTCTGACAACTCCTACAAGCCCTATGCGCTCGGTGCAGCGCGGGCAGGGGTCGATGGCGGGTTCAAGCCCGGCAATGA
CGATCCGTTTTATTCGGGTCTCTCCGACCAGCTGGCTGACAAAGGCTATTTCACCGCTGCCGCAGATGACCTGATCAGCT
GGGCACGCACCGGCTCGCTGATGTGGATGACCTTTGGTCTCGCCTGTTGCGCCGTTGAAATGATGCATGCCGGCAACCCA
CGCTACGATCTTGAGCGCTTCGGCATGGCCCCGCGCGGGTCGCCGCGGCAGTCGGACGTGATGATCGTCGCCGGTACGCT
CACCAACAAGATGGCGCCCGCCCTCCGCAAGGTCTACGACCAGATGCCGGAGCCACGTTATGTGATCTCGATGGGCAGCT
GCGCCAATGGCGGCGGGTATTACCATTACAGCTACAGCGTCGTTCGCGGCTGTGACCGGATCGTTCCTGTGGATATCTAC
ATCCCAGGCTGCCCGCCCACCGCTGAGGCGCTCGTCTACGGCTTCCTTCAGCTGCAGAAGAAGATCCGCCGCGAAGGTTC
GATCGAACGCTGA

Upstream 100 bases:

>100_bases
AGATCGGTATTCTCGGTTTCTGGTCTATGGTGGGTTTCCTGGGCGTCCTGACGGTCGGCTTTATCTATGAATGGCGCCGT
GGCGCGCTGGAGTGGGAATA

Downstream 100 bases:

>100_bases
TGGTGACGATGATGGATACTCAGGCGCTTTCCGATCTTGCCGCCCATATCGAAGGGCGCATGCCCGATGCTGTGCGTAGT
TGGCAGATCCATGTCGGCGA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSDNSYKPYALGAARAGVDGGFKPGNDDPFYSGLSDQLADKGYFTAAADDLISWARTGSLMWMTFGLACCAVEMMHAGNP
RYDLERFGMAPRGSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIY
IPGCPPTAEALVYGFLQLQKKIRREGSIER

Sequences:

>Translated_190_residues
MSDNSYKPYALGAARAGVDGGFKPGNDDPFYSGLSDQLADKGYFTAAADDLISWARTGSLMWMTFGLACCAVEMMHAGNP
RYDLERFGMAPRGSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIY
IPGCPPTAEALVYGFLQLQKKIRREGSIER
>Mature_189_residues
SDNSYKPYALGAARAGVDGGFKPGNDDPFYSGLSDQLADKGYFTAAADDLISWARTGSLMWMTFGLACCAVEMMHAGNPR
YDLERFGMAPRGSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYI
PGCPPTAEALVYGFLQLQKKIRREGSIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=80.9523809523809, Blast_Score=256, Evalue=1e-68,
Organism=Escherichia coli, GI1788624, Length=151, Percent_Identity=48.3443708609272, Blast_Score=155, Evalue=1e-39,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=44.2622950819672, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1788834, Length=122, Percent_Identity=43.4426229508197, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=143, Percent_Identity=69.9300699300699, Blast_Score=227, Evalue=4e-60,
Organism=Drosophila melanogaster, GI18859983, Length=155, Percent_Identity=71.6129032258065, Blast_Score=246, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24642371, Length=155, Percent_Identity=71.6129032258065, Blast_Score=246, Evalue=5e-66,
Organism=Drosophila melanogaster, GI24651058, Length=160, Percent_Identity=71.25, Blast_Score=243, Evalue=7e-65,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_HYPNA (Q0C1E6)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760447.1
- ProteinModelPortal:   Q0C1E6
- SMR:   Q0C1E6
- STRING:   Q0C1E6
- GeneID:   4289104
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1743
- NMPDR:   fig|228405.5.peg.1678
- TIGR:   HNE_1743
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   HYDNERF
- PhylomeDB:   Q0C1E6
- ProtClustDB:   PRK06411
- BioCyc:   HNEP81032:HNE_1743-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20810; Mature: 20679

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNSYKPYALGAARAGVDGGFKPGNDDPFYSGLSDQLADKGYFTAAADDLISWARTGSL
CCCCCCCCEECCHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCH
MWMTFGLACCAVEMMHAGNPRYDLERFGMAPRGSPRQSDVMIVAGTLTNKMAPALRKVYD
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
QMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYIPGCPPTAEALVYGFLQLQK
HCCCCEEEEEECCCCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
KIRREGSIER
HHHHCCCCCC
>Mature Secondary Structure 
SDNSYKPYALGAARAGVDGGFKPGNDDPFYSGLSDQLADKGYFTAAADDLISWARTGSL
CCCCCCCEECCHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCH
MWMTFGLACCAVEMMHAGNPRYDLERFGMAPRGSPRQSDVMIVAGTLTNKMAPALRKVYD
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHH
QMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYIPGCPPTAEALVYGFLQLQK
HCCCCEEEEEECCCCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
KIRREGSIER
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA