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The map label for this gene is thiO/thiG [H]

Identifier: 114799582

GI number: 114799582

Start: 2850826

End: 2851992

Strand: Reverse

Name: thiO/thiG [H]

Synonym: HNE_2691

Alternate gene names: 114799582

Gene position: 2851992-2850826 (Counterclockwise)

Preceding gene: 114798805

Following gene: 114797288

Centisome position: 76.98

GC content: 69.32

Gene sequence:

>1167_bases
ATGTTCAGAGGTTTTCCCCGCAAACCATCCTCTCAGCCGCGCGTCGCCATCATCGGCGCAGGCATTATCGGCCTGAGCCT
GGCGTTCGAGCTGGTGTTCCGGCGCGGCACACGGGTCACCCTTTTTGACACACGGACGCGCGGGCGTGGCGCAAGCTGGG
CCGCAGCCGGCATGATTGCGCCCGCGTTTGAGGCCGCCGCCGAAGAGGGCGTGCATCCGCGCCTGTTTGATCTTTGCCTG
GAAAGCGCGAAGCTGTGGCCGGACTTTGCCGCCGACATCGAGCGGCTGTCGGGCCTGCCATCGGGATTTGAGGCGGCGCC
AGCTCTGGCCGTGGCGCTGGACGAAGGCGAGGCGACGCATCTGGCCGCGATCAGCCAGGTGCTGGCGGCGCGCGGCGTGG
CGCACAGGGCGCTGAACGCGGGCGAGCTGGCGCAGATGGAGCCGGCATTGTCGCCCGATGTGCTGGGCGGGCTGGAGCTG
GAGACCGATACGCGGGTGGACAACCGGCGCACGGTGCGCGCGCTGCTGGCGGCGCTGGAGGCGAGCCCGCGCGTGACCTT
CGTGAGCGGGCCTGCGCCGCTGAAATCCCGAAATGGCCGCGTGCATCTTGAGGGCCATGACGCGATCGTGGCGGCGGCGG
GGTGGGAAACGTCTGTCATCAAGGTGGACGAGCGCGGGCAGCTTTACAGCCTCGTAAACTGGGACACGGCGCTGGACGAT
ATCGACTGCCATGCCGGGCAGATGCTGTCGGTGGTGGCCGGGCCGGGGGCGCCGCAGCGGGTGGTGCGCGCGGGGCATGT
GTATCTGGTGCCGCGCGAGGGGCATGTTGTGATCGGGGCAACGATGGAGCCTGACCGGGTGATTGACGCGCCCGAAGCCG
ACGTGATCGAGGCATTGCGGCTGGAGGGCGTGAAGCTCTGCCCGGCGCTGGCGGGGGCGGCGGTGACGGAGAGCTGGGCC
GGGGTGCGGCCGGGCACGCCTGACCATGCGCCGTTCCTTGGGGAGACCGTGACGCCGGGGCTTTATGTGGCGGCGGGGCA
TTATCGCAACGGCATCCTGCTGGCGCCGGTGACCGCCCAGATCCTTGCAGATCAGATTTTCGGGCAGGATACGGGCGAGC
TTGCCGCTGCGTTCACCACGCGCCGGGCCTATTCCGCGACGGCCTGA

Upstream 100 bases:

>100_bases
GCTTTCTGCGCATTCTGGATGACCGAGACCGGGCTTCAGGTCGCCCATTTTCCGTACTGAGCCACTTTCCCGCCTCTGAA
GGGGCGCCTAGAAGGCGGCC

Downstream 100 bases:

>100_bases
CGCTGGACGTGCCCCCTCTCGCGCCTTAAGTCTCGGCGCCAAAGGGAGCTTACGCCATGCATGTTATCCAGCCGATGACC
GAAGATCAGGCCGCCATCCG

Product: putative glycine oxidase

Products: NA

Alternate protein names: Probable FAD-dependent glycine oxidase; Thiazole synthase [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MFRGFPRKPSSQPRVAIIGAGIIGLSLAFELVFRRGTRVTLFDTRTRGRGASWAAAGMIAPAFEAAAEEGVHPRLFDLCL
ESAKLWPDFAADIERLSGLPSGFEAAPALAVALDEGEATHLAAISQVLAARGVAHRALNAGELAQMEPALSPDVLGGLEL
ETDTRVDNRRTVRALLAALEASPRVTFVSGPAPLKSRNGRVHLEGHDAIVAAAGWETSVIKVDERGQLYSLVNWDTALDD
IDCHAGQMLSVVAGPGAPQRVVRAGHVYLVPREGHVVIGATMEPDRVIDAPEADVIEALRLEGVKLCPALAGAAVTESWA
GVRPGTPDHAPFLGETVTPGLYVAAGHYRNGILLAPVTAQILADQIFGQDTGELAAAFTTRRAYSATA

Sequences:

>Translated_388_residues
MFRGFPRKPSSQPRVAIIGAGIIGLSLAFELVFRRGTRVTLFDTRTRGRGASWAAAGMIAPAFEAAAEEGVHPRLFDLCL
ESAKLWPDFAADIERLSGLPSGFEAAPALAVALDEGEATHLAAISQVLAARGVAHRALNAGELAQMEPALSPDVLGGLEL
ETDTRVDNRRTVRALLAALEASPRVTFVSGPAPLKSRNGRVHLEGHDAIVAAAGWETSVIKVDERGQLYSLVNWDTALDD
IDCHAGQMLSVVAGPGAPQRVVRAGHVYLVPREGHVVIGATMEPDRVIDAPEADVIEALRLEGVKLCPALAGAAVTESWA
GVRPGTPDHAPFLGETVTPGLYVAAGHYRNGILLAPVTAQILADQIFGQDTGELAAAFTTRRAYSATA
>Mature_388_residues
MFRGFPRKPSSQPRVAIIGAGIIGLSLAFELVFRRGTRVTLFDTRTRGRGASWAAAGMIAPAFEAAAEEGVHPRLFDLCL
ESAKLWPDFAADIERLSGLPSGFEAAPALAVALDEGEATHLAAISQVLAARGVAHRALNAGELAQMEPALSPDVLGGLEL
ETDTRVDNRRTVRALLAALEASPRVTFVSGPAPLKSRNGRVHLEGHDAIVAAAGWETSVIKVDERGQLYSLVNWDTALDD
IDCHAGQMLSVVAGPGAPQRVVRAGHVYLVPREGHVVIGATMEPDRVIDAPEADVIEALRLEGVKLCPALAGAAVTESWA
GVRPGTPDHAPFLGETVTPGLYVAAGHYRNGILLAPVTAQILADQIFGQDTGELAAAFTTRRAYSATA

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006076
- InterPro:   IPR012727
- InterPro:   IPR008867 [H]

Pfam domain/function: PF01266 DAO; PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 40869; Mature: 40869

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRGFPRKPSSQPRVAIIGAGIIGLSLAFELVFRRGTRVTLFDTRTRGRGASWAAAGMIA
CCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHH
PAFEAAAEEGVHPRLFDLCLESAKLWPDFAADIERLSGLPSGFEAAPALAVALDEGEATH
HHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHH
LAAISQVLAARGVAHRALNAGELAQMEPALSPDVLGGLELETDTRVDNRRTVRALLAALE
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHCCEEECCCCCCCCHHHHHHHHHHHC
ASPRVTFVSGPAPLKSRNGRVHLEGHDAIVAAAGWETSVIKVDERGQLYSLVNWDTALDD
CCCCEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEEEEEECCCCCEEEEECCCCCCCC
IDCHAGQMLSVVAGPGAPQRVVRAGHVYLVPREGHVVIGATMEPDRVIDAPEADVIEALR
CCCCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHH
LEGVKLCPALAGAAVTESWAGVRPGTPDHAPFLGETVTPGLYVAAGHYRNGILLAPVTAQ
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCHHHH
ILADQIFGQDTGELAAAFTTRRAYSATA
HHHHHHHCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFRGFPRKPSSQPRVAIIGAGIIGLSLAFELVFRRGTRVTLFDTRTRGRGASWAAAGMIA
CCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHH
PAFEAAAEEGVHPRLFDLCLESAKLWPDFAADIERLSGLPSGFEAAPALAVALDEGEATH
HHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHH
LAAISQVLAARGVAHRALNAGELAQMEPALSPDVLGGLELETDTRVDNRRTVRALLAALE
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHCCEEECCCCCCCCHHHHHHHHHHHC
ASPRVTFVSGPAPLKSRNGRVHLEGHDAIVAAAGWETSVIKVDERGQLYSLVNWDTALDD
CCCCEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEEEEEECCCCCEEEEECCCCCCCC
IDCHAGQMLSVVAGPGAPQRVVRAGHVYLVPREGHVVIGATMEPDRVIDAPEADVIEALR
CCCCCCCEEEEEECCCCHHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHH
LEGVKLCPALAGAAVTESWAGVRPGTPDHAPFLGETVTPGLYVAAGHYRNGILLAPVTAQ
HCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCHHHH
ILADQIFGQDTGELAAAFTTRRAYSATA
HHHHHHHCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]