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The map label for this gene is dus [H]

Identifier: 114799466

GI number: 114799466

Start: 2090531

End: 2091568

Strand: Reverse

Name: dus [H]

Synonym: HNE_2013

Alternate gene names: 114799466

Gene position: 2091568-2090531 (Counterclockwise)

Preceding gene: 114798642

Following gene: 114800221

Centisome position: 56.45

GC content: 65.61

Gene sequence:

>1038_bases
TTGTGCGCTTGCGAAAAAATGAAAAGTGAGAAACAAAAGGATATGATCAATTTTGAGGCACCTCGGGTCTGGCTGGCGCC
AATGTCCGGCGCAACGGACGCCCCCATGCGGCGGCAAGCGGTGCATTTCGGCGCCCCGGCCGTAGTGTCGGAGATGGTCG
CCGGCGAAATGCTCGCCAAGGCCCGGGCAGACGTTGTGCGCCGCACATGCCGCCATGAAGGTGCAGGCTACTGGATCGTC
CAGCTGGCCGCCCGCCGCCCTGAAGACATGCTCGCAGGCGCCGTTCTGATGGCCGAGGCGGGCGTCGATGTCATCGACAT
CAATATGGGCTGCCCCTCCAAACAGGTCACCGGTGGCCAGTCCGGCTCCGCGCTGATGCGGGATCTGCCGCTGGCAGGCG
AAATCATCGACGCCGCCCTCCAGGGCGCTGCAGGCCGTCCCGTCACGCTGAAAATGCGCCTCGGCTGGGACGATGAAAAC
CTCAACGCCCCCGAGCTCGCTGTCATGGCGCAAGACCGCGGCGTGCAAATGCTCACCGTCCACGGCCGCACGCGCTGCCA
GTTCTACACAGGCGGCGCCAACTGGGCTGCTGTCCGCGCCACTGTGGACGCCGTCAGCCTGCCCGTCATCGTCAATGGCG
ACATCTCCGATACCGCCTCGGCCCGTGAGGCGCTCGCGCAAAGCGGCGCTTACGGCGTCATGCTCGGCCGCGCGGCCATG
GGCCGGCCCTGGCTGGTGGGCGAAGTCGCCGCCGCCTTGGAAGGCCGCGCCTGGCGCAGGCCCGCCCTCAATGAACAGCT
CGCCAGCCTCTGCGAGCAGATCACTGACTCGATCGATCTCTATGGGGCCAGCCTTGGCCTCAGGATGGTGCGCAAGCATG
TCTCTGCCGCCATCGACGAACTGGAAACTGACCTGCCGGCCACAGAACGCCGGCAGCTGCGCGCCGATCTCTGCCGGATA
GGGGACGCCGGAGAGCTGATCGCTGCGCTGCGTAAGGTCTATCTTGGAAAGGTGTTGGAGAAGGCGGTGTCCTCATGA

Upstream 100 bases:

>100_bases
ATTATGGCGACGGTCTCGGTCATGATCTGGCTTGTAGGCGACCCGCACGCGGCTGGCTACTCTCCGAAATGTCCCGAAAA
GGGAAAGAAATGCCCATGAT

Downstream 100 bases:

>100_bases
CGACTTTGACTGGCGAGCGGCCCTCCGGCCTCGCAGATCTGTCCCCCATCGCGCTGCTGCATGTTGATCAGCGCCGCCGC
ATCGCGAATGTGAACCCGGC

Product: putative tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MCACEKMKSEKQKDMINFEAPRVWLAPMSGATDAPMRRQAVHFGAPAVVSEMVAGEMLAKARADVVRRTCRHEGAGYWIV
QLAARRPEDMLAGAVLMAEAGVDVIDINMGCPSKQVTGGQSGSALMRDLPLAGEIIDAALQGAAGRPVTLKMRLGWDDEN
LNAPELAVMAQDRGVQMLTVHGRTRCQFYTGGANWAAVRATVDAVSLPVIVNGDISDTASAREALAQSGAYGVMLGRAAM
GRPWLVGEVAAALEGRAWRRPALNEQLASLCEQITDSIDLYGASLGLRMVRKHVSAAIDELETDLPATERRQLRADLCRI
GDAGELIAALRKVYLGKVLEKAVSS

Sequences:

>Translated_345_residues
MCACEKMKSEKQKDMINFEAPRVWLAPMSGATDAPMRRQAVHFGAPAVVSEMVAGEMLAKARADVVRRTCRHEGAGYWIV
QLAARRPEDMLAGAVLMAEAGVDVIDINMGCPSKQVTGGQSGSALMRDLPLAGEIIDAALQGAAGRPVTLKMRLGWDDEN
LNAPELAVMAQDRGVQMLTVHGRTRCQFYTGGANWAAVRATVDAVSLPVIVNGDISDTASAREALAQSGAYGVMLGRAAM
GRPWLVGEVAAALEGRAWRRPALNEQLASLCEQITDSIDLYGASLGLRMVRKHVSAAIDELETDLPATERRQLRADLCRI
GDAGELIAALRKVYLGKVLEKAVSS
>Mature_345_residues
MCACEKMKSEKQKDMINFEAPRVWLAPMSGATDAPMRRQAVHFGAPAVVSEMVAGEMLAKARADVVRRTCRHEGAGYWIV
QLAARRPEDMLAGAVLMAEAGVDVIDINMGCPSKQVTGGQSGSALMRDLPLAGEIIDAALQGAAGRPVTLKMRLGWDDEN
LNAPELAVMAQDRGVQMLTVHGRTRCQFYTGGANWAAVRATVDAVSLPVIVNGDISDTASAREALAQSGAYGVMLGRAAM
GRPWLVGEVAAALEGRAWRRPALNEQLASLCEQITDSIDLYGASLGLRMVRKHVSAAIDELETDLPATERRQLRADLCRI
GDAGELIAALRKVYLGKVLEKAVSS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=33.0543933054393, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI239788462, Length=239, Percent_Identity=33.0543933054393, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI40807366, Length=219, Percent_Identity=32.4200913242009, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI8923374, Length=248, Percent_Identity=33.4677419354839, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789660, Length=314, Percent_Identity=37.5796178343949, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1788462, Length=234, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=243, Percent_Identity=30.8641975308642, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=239, Percent_Identity=26.7782426778243, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=180, Percent_Identity=32.7777777777778, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=234, Percent_Identity=30.7692307692308, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323437, Length=224, Percent_Identity=27.2321428571429, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24585320, Length=252, Percent_Identity=33.7301587301587, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24580595, Length=239, Percent_Identity=30.5439330543933, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19920448, Length=239, Percent_Identity=30.5439330543933, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI19921524, Length=259, Percent_Identity=25.8687258687259, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45549423, Length=253, Percent_Identity=28.4584980237154, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36938; Mature: 36938

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCACEKMKSEKQKDMINFEAPRVWLAPMSGATDAPMRRQAVHFGAPAVVSEMVAGEMLAK
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
ARADVVRRTCRHEGAGYWIVQLAARRPEDMLAGAVLMAEAGVDVIDINMGCPSKQVTGGQ
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
SGSALMRDLPLAGEIIDAALQGAAGRPVTLKMRLGWDDENLNAPELAVMAQDRGVQMLTV
CCCHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEEE
HGRTRCQFYTGGANWAAVRATVDAVSLPVIVNGDISDTASAREALAQSGAYGVMLGRAAM
CCCEEEEEEECCCCEEEHEEEHHHEEECEEECCCCCCHHHHHHHHHHCCCCEEEECHHHC
GRPWLVGEVAAALEGRAWRRPALNEQLASLCEQITDSIDLYGASLGLRMVRKHVSAAIDE
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETDLPATERRQLRADLCRIGDAGELIAALRKVYLGKVLEKAVSS
HHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCACEKMKSEKQKDMINFEAPRVWLAPMSGATDAPMRRQAVHFGAPAVVSEMVAGEMLAK
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
ARADVVRRTCRHEGAGYWIVQLAARRPEDMLAGAVLMAEAGVDVIDINMGCPSKQVTGGQ
HHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCC
SGSALMRDLPLAGEIIDAALQGAAGRPVTLKMRLGWDDENLNAPELAVMAQDRGVQMLTV
CCCHHHHHCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEEE
HGRTRCQFYTGGANWAAVRATVDAVSLPVIVNGDISDTASAREALAQSGAYGVMLGRAAM
CCCEEEEEEECCCCEEEHEEEHHHEEECEEECCCCCCHHHHHHHHHHCCCCEEEECHHHC
GRPWLVGEVAAALEGRAWRRPALNEQLASLCEQITDSIDLYGASLGLRMVRKHVSAAIDE
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETDLPATERRQLRADLCRIGDAGELIAALRKVYLGKVLEKAVSS
HHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]