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The map label for this gene is oppA [H]

Identifier: 114799455

GI number: 114799455

Start: 3032384

End: 3034009

Strand: Reverse

Name: oppA [H]

Synonym: HNE_2874

Alternate gene names: 114799455

Gene position: 3034009-3032384 (Counterclockwise)

Preceding gene: 114798441

Following gene: 114798601

Centisome position: 81.89

GC content: 62.3

Gene sequence:

>1626_bases
ATGAAAATGAAATCCCTGCTGGCCGGCGCGGCCAGTGTGATGATGGCCGTTACGCTGGCCGCCTGCGGCGGCGGTGGCAG
CTCTGGCGGCGAGGATGTTCCGACGCTGCGGCGCGGCATCTCCGCCAAAGTCGACACGCTGGACCCGCACAAATCCTCGG
CCGCCTGGGAAAACATTGTCATCGGCGACATGATGATCGGTCTGACGACCGATAATGCCGACGGCAAGCCGGTGCCGGGC
ATGGCGCAGAGCTGGGAGGTCGATGACACCGGCACGGTGTGGACCTTCAAGCTGGGCGACTACACCTGGTCAGATGGCAC
GCCGGTCACGGCAGAAGACTTCGTCTACGCCTTCCGCCGCATTCAGGACCCCACAGTTGCCTCGCAATACGCCTCCATGC
TCTGGCTGGTGAAGAATGCCGAGAAGGTGAACAATGGCGACCTGCCGCCCGAGGAAATGGGTGTGCGCGCGGTGGACGCG
AAAACCTTCGAGATCACGCTGGAATATCCCGCGCCCTACCTGCCGGGGCTTCTGTCCCACTACACCACCTTTCCCGTGCC
GCGTCAGGCAGTCGAGACGTTCGGCGACGCCTGGATTCAGCCGGACAATATCGTGGTCAACGGCCCCTACAAACTTGTCT
ACTGGCGCACCGGCGACCAGCTGGTCGCCGAGAAGAACCCGACCGGGTTTGGCGCCGCAGAAGCCTGCTTTGACCGCGTC
GTCTATTTCGAGATCGAGAATGAAGCCGCTTATGAGAACAAGATCTCGGCGGGCGAGCTGGACATCAACAATGTATTCTC
CGGCCCGCGCCAGAGCGAGATCGAAGCCAAGTTTCCCGGCTGGATACGCACCGGGCCCGGTCTTCTGACCACGTACTGGA
CGTTCAACCAGCAGCGCGCGCCGTTTGATGATGTGCGGGTGCGCAAGGCGCTCTCGATGGCGCTCGACCGCGAATTCATG
GTGCGCAATGTGATGACGCCGGGTTTCCAGGCGGCTTATTCCTTCGTGCCGCCGGAGATCAGCAATTATGACGTGCCGCG
CCCGGCCGTCTCGTGGGAGGACATGCCGCGGGAGGAGCGCCTGGTAGAGGCGCGGCGCCTGCTGGAAGAAGCCGGTTTTG
GCCCGTCCAATCCGCTGAAGTTTGAGTTCACTCACCGCTCGACCGGCGACAATCCGAAGGTTGCCCCCGTGGCCCAGCAG
AACTGGGCGGAGATTGCCGATTGGGTGGAGCCGGTCATCGTGCGCCAGGACACCAAGGTGCTGTATGCGCGCCTGCGCCA
GAACGACTTCCAGGTGTCGGATGCGGCCTGGCTCGCCGACTATGACGATCCGATCAACTTCCTCTACCTGCTGCAATCGA
CCACGGGCCAGCAGAACTATGGCCGCTATGCCAATGCCGAGTATGACGCGCTGCTTGCCCAGTCGAATGCTGAGCTTGAT
CTGGCCAAGCGTGCGGAAATCTTTGCCAAGGCCGAGGCGCTGATGCTCGAAGAGTATCCGATCACGCCGATGTGGGTGCA
GGTTACGCAGAACCTTGTCGATCCCACGCTCACGGGCTGGGAGGATAATGCCAAGGATATCCACCGCTCGCGCTTTCTCT
GCCGCAGCGGGGCAGGGGCCGAATAA

Upstream 100 bases:

>100_bases
CGTTCACTTATATGCGGGCAAGTCTTTGCTTTTGTTTGCCCGTCTGGCACCTGTTCTGGCAAATCTGAAGCGATACAGGA
AGATCAAGGAGGCATAACCG

Downstream 100 bases:

>100_bases
GCCCTGCCGCCCGGCAGGACGGGCAATATGAAGTCTGGGCCGCAGGGATCGAGGGATGCCTGCGGCCTTTTCTATCTCGG
CAGGCGCGGCGGGTGAGCGT

Product: oligopeptide ABC transporter periplasmic oligopeptide-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MKMKSLLAGAASVMMAVTLAACGGGGSSGGEDVPTLRRGISAKVDTLDPHKSSAAWENIVIGDMMIGLTTDNADGKPVPG
MAQSWEVDDTGTVWTFKLGDYTWSDGTPVTAEDFVYAFRRIQDPTVASQYASMLWLVKNAEKVNNGDLPPEEMGVRAVDA
KTFEITLEYPAPYLPGLLSHYTTFPVPRQAVETFGDAWIQPDNIVVNGPYKLVYWRTGDQLVAEKNPTGFGAAEACFDRV
VYFEIENEAAYENKISAGELDINNVFSGPRQSEIEAKFPGWIRTGPGLLTTYWTFNQQRAPFDDVRVRKALSMALDREFM
VRNVMTPGFQAAYSFVPPEISNYDVPRPAVSWEDMPREERLVEARRLLEEAGFGPSNPLKFEFTHRSTGDNPKVAPVAQQ
NWAEIADWVEPVIVRQDTKVLYARLRQNDFQVSDAAWLADYDDPINFLYLLQSTTGQQNYGRYANAEYDALLAQSNAELD
LAKRAEIFAKAEALMLEEYPITPMWVQVTQNLVDPTLTGWEDNAKDIHRSRFLCRSGAGAE

Sequences:

>Translated_541_residues
MKMKSLLAGAASVMMAVTLAACGGGGSSGGEDVPTLRRGISAKVDTLDPHKSSAAWENIVIGDMMIGLTTDNADGKPVPG
MAQSWEVDDTGTVWTFKLGDYTWSDGTPVTAEDFVYAFRRIQDPTVASQYASMLWLVKNAEKVNNGDLPPEEMGVRAVDA
KTFEITLEYPAPYLPGLLSHYTTFPVPRQAVETFGDAWIQPDNIVVNGPYKLVYWRTGDQLVAEKNPTGFGAAEACFDRV
VYFEIENEAAYENKISAGELDINNVFSGPRQSEIEAKFPGWIRTGPGLLTTYWTFNQQRAPFDDVRVRKALSMALDREFM
VRNVMTPGFQAAYSFVPPEISNYDVPRPAVSWEDMPREERLVEARRLLEEAGFGPSNPLKFEFTHRSTGDNPKVAPVAQQ
NWAEIADWVEPVIVRQDTKVLYARLRQNDFQVSDAAWLADYDDPINFLYLLQSTTGQQNYGRYANAEYDALLAQSNAELD
LAKRAEIFAKAEALMLEEYPITPMWVQVTQNLVDPTLTGWEDNAKDIHRSRFLCRSGAGAE
>Mature_541_residues
MKMKSLLAGAASVMMAVTLAACGGGGSSGGEDVPTLRRGISAKVDTLDPHKSSAAWENIVIGDMMIGLTTDNADGKPVPG
MAQSWEVDDTGTVWTFKLGDYTWSDGTPVTAEDFVYAFRRIQDPTVASQYASMLWLVKNAEKVNNGDLPPEEMGVRAVDA
KTFEITLEYPAPYLPGLLSHYTTFPVPRQAVETFGDAWIQPDNIVVNGPYKLVYWRTGDQLVAEKNPTGFGAAEACFDRV
VYFEIENEAAYENKISAGELDINNVFSGPRQSEIEAKFPGWIRTGPGLLTTYWTFNQQRAPFDDVRVRKALSMALDREFM
VRNVMTPGFQAAYSFVPPEISNYDVPRPAVSWEDMPREERLVEARRLLEEAGFGPSNPLKFEFTHRSTGDNPKVAPVAQQ
NWAEIADWVEPVIVRQDTKVLYARLRQNDFQVSDAAWLADYDDPINFLYLLQSTTGQQNYGRYANAEYDALLAQSNAELD
LAKRAEIFAKAEALMLEEYPITPMWVQVTQNLVDPTLTGWEDNAKDIHRSRFLCRSGAGAE

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=522, Percent_Identity=35.2490421455939, Blast_Score=314, Evalue=1e-86,
Organism=Escherichia coli, GI87081878, Length=502, Percent_Identity=34.2629482071713, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1789397, Length=485, Percent_Identity=32.9896907216495, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1789966, Length=468, Percent_Identity=22.8632478632479, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787762, Length=430, Percent_Identity=23.2558139534884, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787551, Length=494, Percent_Identity=24.4939271255061, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787052, Length=495, Percent_Identity=23.2323232323232, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60273; Mature: 60273

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKSLLAGAASVMMAVTLAACGGGGSSGGEDVPTLRRGISAKVDTLDPHKSSAAWENIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHEE
IGDMMIGLTTDNADGKPVPGMAQSWEVDDTGTVWTFKLGDYTWSDGTPVTAEDFVYAFRR
EEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCEECCCCCCCCHHHHHHHHHH
IQDPTVASQYASMLWLVKNAEKVNNGDLPPEEMGVRAVDAKTFEITLEYPAPYLPGLLSH
CCCCHHHHHHHHHHHHHHCHHHCCCCCCCHHHHCCEEEECEEEEEEEECCCCCCHHHHHH
YTTFPVPRQAVETFGDAWIQPDNIVVNGPYKLVYWRTGDQLVAEKNPTGFGAAEACFDRV
HCCCCCCHHHHHHHHHCCCCCCCEEEECCEEEEEEECCCEEEECCCCCCCCHHHHHHCEE
VYFEIENEAAYENKISAGELDINNVFSGPRQSEIEAKFPGWIRTGPGLLTTYWTFNQQRA
EEEEECCCCHHCCCCCCCCEEHHHHCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCC
PFDDVRVRKALSMALDREFMVRNVMTPGFQAAYSFVPPEISNYDVPRPAVSWEDMPREER
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
LVEARRLLEEAGFGPSNPLKFEFTHRSTGDNPKVAPVAQQNWAEIADWVEPVIVRQDTKV
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCHHH
LYARLRQNDFQVSDAAWLADYDDPINFLYLLQSTTGQQNYGRYANAEYDALLAQSNAELD
HHHHHHCCCCEECCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHEECCCCCCC
LAKRAEIFAKAEALMLEEYPITPMWVQVTQNLVDPTLTGWEDNAKDIHRSRFLCRSGAGA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCC
E
C
>Mature Secondary Structure
MKMKSLLAGAASVMMAVTLAACGGGGSSGGEDVPTLRRGISAKVDTLDPHKSSAAWENIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHEE
IGDMMIGLTTDNADGKPVPGMAQSWEVDDTGTVWTFKLGDYTWSDGTPVTAEDFVYAFRR
EEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCEECCCCCCCCHHHHHHHHHH
IQDPTVASQYASMLWLVKNAEKVNNGDLPPEEMGVRAVDAKTFEITLEYPAPYLPGLLSH
CCCCHHHHHHHHHHHHHHCHHHCCCCCCCHHHHCCEEEECEEEEEEEECCCCCCHHHHHH
YTTFPVPRQAVETFGDAWIQPDNIVVNGPYKLVYWRTGDQLVAEKNPTGFGAAEACFDRV
HCCCCCCHHHHHHHHHCCCCCCCEEEECCEEEEEEECCCEEEECCCCCCCCHHHHHHCEE
VYFEIENEAAYENKISAGELDINNVFSGPRQSEIEAKFPGWIRTGPGLLTTYWTFNQQRA
EEEEECCCCHHCCCCCCCCEEHHHHCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCC
PFDDVRVRKALSMALDREFMVRNVMTPGFQAAYSFVPPEISNYDVPRPAVSWEDMPREER
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
LVEARRLLEEAGFGPSNPLKFEFTHRSTGDNPKVAPVAQQNWAEIADWVEPVIVRQDTKV
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCHHH
LYARLRQNDFQVSDAAWLADYDDPINFLYLLQSTTGQQNYGRYANAEYDALLAQSNAELD
HHHHHHCCCCEECCEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHEECCCCCCC
LAKRAEIFAKAEALMLEEYPITPMWVQVTQNLVDPTLTGWEDNAKDIHRSRFLCRSGAGA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]