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The map label for this gene is glnG [H]

Identifier: 114799444

GI number: 114799444

Start: 2088097

End: 2089470

Strand: Reverse

Name: glnG [H]

Synonym: HNE_2011

Alternate gene names: 114799444

Gene position: 2089470-2088097 (Counterclockwise)

Preceding gene: 114800221

Following gene: 114800247

Centisome position: 56.4

GC content: 64.77

Gene sequence:

>1374_bases
ATGGCCGACAAGACTGTTCTCCTCGCTGAAGACGATGCCAGCATCCGGCTCGTCGTGAACCAGACCCTGGTGTCCGCAGG
CTATGAGGTGCGCGCCACCAGTTCCCCGGACGCGCTCCAGCGCTGGGTGCGCAATGGCGAGGGCGATGTTGTCGTCACCG
ACGTCTATCTCGGCGACACGTCGATCTTTGATCTTCTGCCCTCGATGAAGCTCGCCCGGCCGGATCTGCCTTTCATCGTG
ATGAGCGGCCAGAACACCATCCTGACCGCCGCTTCTGCTGCCGAGCATGGCGCCTTCGATTACCTCCCCAAACCCTTCGA
CATTGATGTCCTATCCAGCCTCGTTCGCCGTGCGCTCAAATCCTCGCCGGCGCCGGAGCGCGCCATCCACCCTGACGCCC
AGAAGGCGGTCGCCGATGCGGGCCTGCCGCTGATCGGCCGGTCGGATGCGATGCAGGAGGTCTACCGCCTCGTCGCCAAG
GTGATGAACACCGACCTCACCGTTCTGATCGAAGGCGAAAGCGGCACCGGCAAGGAACTCGCCGCCCGCGCCATCCACCA
GCTCGGCCAGTCCAAGCGCGGCCAGTTCATCGCGCTCGACCTTGCCGCTATGCCGCCGGCCGAAATCAACCGCGAACTCT
TCGGCACAGATGGCAATGACGGCGCGGTTCACCTGAAAGGTGGCACGCTCTATCTCGACGAGATTGGCGACCTTCCCGCT
GAAGCCCAGGCCCAACTCGTCAAGCTCCTGCGCGAGCCCGGCGGCGCCCGCCTCATCGCCTCCACCCGGCGCAATCTTGG
CCGCCTCGTGGAAGACGGCAAGTTCCGCGAAGACCTTTATTACCGCCTCAACGTGATGCGCCTTGCCATCCCGCCGCTGC
GCCAGCGCAAGGAAGACATCCCCGAACTCGCCCGCGCCTTCCTGATCCGCGCCCAGCGCCAGGGCCTCGCCGCGCGCACC
TTCGACAAGTCCGCGCTCGACCTGATGGCCGCCTATGACTGGCCGGGCAACGTGCGCGAGCTCGAAAACCTCGTTCTGCG
CCTTGCAGTGCTCAGCTCCGATTCGGTCATCACGGTGATGGATGTCGAGCGCGAGATGCGGATGGGCGTCGCCGAGCAGA
CCGGCGCCAGTCTCGGCTTCGAGGCCGAAATCGAGGGGCTCCTCCACCGCTATGTGATGGGCGATCTGGTTTCCGGCGGT
GAAGGGGAGGGCTCAAATGTCCACGAATCCGTTGTTAACCGCGTGGAACGGCCGCTGATCCGGCTCGCGCTGTCGGTTAC
CTCGGGCAACAAGGTTCGCGCGGCGCAGCTATTGGGGATGAACCGGAACACGCTGCGGGCCAAGATCAACGCCCTCGGCA
TTGCAGAAGACTGA

Upstream 100 bases:

>100_bases
GCGAAGTCGTCAATGCCCATGGCGGCCAGATCCGCGTCGACAGCCAGCCGGGCGCTACCCGTTTCACCATTCTGTTGCCG
CTTTCGGAGGCGTAAATCTC

Downstream 100 bases:

>100_bases
TTGACGACACCCCAATCTGTGGCTCAAGTGCACCACTGTTGCTGGCAGGACACTTAACTGTGTCCTGATTGCAACGGGGC
GGGGCCCCAACAGAGCCGGA

Product: nitrogen regulation protein NR(I)

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MADKTVLLAEDDASIRLVVNQTLVSAGYEVRATSSPDALQRWVRNGEGDVVVTDVYLGDTSIFDLLPSMKLARPDLPFIV
MSGQNTILTAASAAEHGAFDYLPKPFDIDVLSSLVRRALKSSPAPERAIHPDAQKAVADAGLPLIGRSDAMQEVYRLVAK
VMNTDLTVLIEGESGTGKELAARAIHQLGQSKRGQFIALDLAAMPPAEINRELFGTDGNDGAVHLKGGTLYLDEIGDLPA
EAQAQLVKLLREPGGARLIASTRRNLGRLVEDGKFREDLYYRLNVMRLAIPPLRQRKEDIPELARAFLIRAQRQGLAART
FDKSALDLMAAYDWPGNVRELENLVLRLAVLSSDSVITVMDVEREMRMGVAEQTGASLGFEAEIEGLLHRYVMGDLVSGG
EGEGSNVHESVVNRVERPLIRLALSVTSGNKVRAAQLLGMNRNTLRAKINALGIAED

Sequences:

>Translated_457_residues
MADKTVLLAEDDASIRLVVNQTLVSAGYEVRATSSPDALQRWVRNGEGDVVVTDVYLGDTSIFDLLPSMKLARPDLPFIV
MSGQNTILTAASAAEHGAFDYLPKPFDIDVLSSLVRRALKSSPAPERAIHPDAQKAVADAGLPLIGRSDAMQEVYRLVAK
VMNTDLTVLIEGESGTGKELAARAIHQLGQSKRGQFIALDLAAMPPAEINRELFGTDGNDGAVHLKGGTLYLDEIGDLPA
EAQAQLVKLLREPGGARLIASTRRNLGRLVEDGKFREDLYYRLNVMRLAIPPLRQRKEDIPELARAFLIRAQRQGLAART
FDKSALDLMAAYDWPGNVRELENLVLRLAVLSSDSVITVMDVEREMRMGVAEQTGASLGFEAEIEGLLHRYVMGDLVSGG
EGEGSNVHESVVNRVERPLIRLALSVTSGNKVRAAQLLGMNRNTLRAKINALGIAED
>Mature_456_residues
ADKTVLLAEDDASIRLVVNQTLVSAGYEVRATSSPDALQRWVRNGEGDVVVTDVYLGDTSIFDLLPSMKLARPDLPFIVM
SGQNTILTAASAAEHGAFDYLPKPFDIDVLSSLVRRALKSSPAPERAIHPDAQKAVADAGLPLIGRSDAMQEVYRLVAKV
MNTDLTVLIEGESGTGKELAARAIHQLGQSKRGQFIALDLAAMPPAEINRELFGTDGNDGAVHLKGGTLYLDEIGDLPAE
AQAQLVKLLREPGGARLIASTRRNLGRLVEDGKFREDLYYRLNVMRLAIPPLRQRKEDIPELARAFLIRAQRQGLAARTF
DKSALDLMAAYDWPGNVRELENLVLRLAVLSSDSVITVMDVEREMRMGVAEQTGASLGFEAEIEGLLHRYVMGDLVSGGE
GEGSNVHESVVNRVERPLIRLALSVTSGNKVRAAQLLGMNRNTLRAKINALGIAED

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=477, Percent_Identity=35.8490566037736, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1790437, Length=470, Percent_Identity=36.5957446808511, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1788550, Length=475, Percent_Identity=33.0526315789474, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1788905, Length=463, Percent_Identity=33.0453563714903, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87082117, Length=350, Percent_Identity=35.4285714285714, Blast_Score=182, Evalue=6e-47,
Organism=Escherichia coli, GI1789087, Length=239, Percent_Identity=41.4225941422594, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1789233, Length=332, Percent_Identity=34.9397590361446, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1786524, Length=252, Percent_Identity=36.9047619047619, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87082152, Length=250, Percent_Identity=36.8, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787583, Length=285, Percent_Identity=34.0350877192982, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI87081872, Length=337, Percent_Identity=30.2670623145401, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI87081858, Length=316, Percent_Identity=28.4810126582279, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789828, Length=231, Percent_Identity=35.4978354978355, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49677; Mature: 49545

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKTVLLAEDDASIRLVVNQTLVSAGYEVRATSSPDALQRWVRNGEGDVVVTDVYLGDT
CCCCEEEEECCCCCEEEEEEHHHHHCCCEEEECCCHHHHHHHHHCCCCCEEEEEEEECCC
SIFDLLPSMKLARPDLPFIVMSGQNTILTAASAAEHGAFDYLPKPFDIDVLSSLVRRALK
HHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SSPAPERAIHPDAQKAVADAGLPLIGRSDAMQEVYRLVAKVMNTDLTVLIEGESGTGKEL
CCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
AARAIHQLGQSKRGQFIALDLAAMPPAEINRELFGTDGNDGAVHLKGGTLYLDEIGDLPA
HHHHHHHHCCCCCCCEEEEEECCCCHHHHCHHHCCCCCCCCEEEEECCEEEEECCCCCCH
EAQAQLVKLLREPGGARLIASTRRNLGRLVEDGKFREDLYYRLNVMRLAIPPLRQRKEDI
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCHHHHHHHH
PELARAFLIRAQRQGLAARTFDKSALDLMAAYDWPGNVRELENLVLRLAVLSSDSVITVM
HHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCEEEEE
DVEREMRMGVAEQTGASLGFEAEIEGLLHRYVMGDLVSGGEGEGSNVHESVVNRVERPLI
ECHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
RLALSVTSGNKVRAAQLLGMNRNTLRAKINALGIAED
HHEEEECCCCCEEHHHHHCCCCHHHHEEHHEECCCCC
>Mature Secondary Structure 
ADKTVLLAEDDASIRLVVNQTLVSAGYEVRATSSPDALQRWVRNGEGDVVVTDVYLGDT
CCCEEEEECCCCCEEEEEEHHHHHCCCEEEECCCHHHHHHHHHCCCCCEEEEEEEECCC
SIFDLLPSMKLARPDLPFIVMSGQNTILTAASAAEHGAFDYLPKPFDIDVLSSLVRRALK
HHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SSPAPERAIHPDAQKAVADAGLPLIGRSDAMQEVYRLVAKVMNTDLTVLIEGESGTGKEL
CCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
AARAIHQLGQSKRGQFIALDLAAMPPAEINRELFGTDGNDGAVHLKGGTLYLDEIGDLPA
HHHHHHHHCCCCCCCEEEEEECCCCHHHHCHHHCCCCCCCCEEEEECCEEEEECCCCCCH
EAQAQLVKLLREPGGARLIASTRRNLGRLVEDGKFREDLYYRLNVMRLAIPPLRQRKEDI
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHEECCCCHHHHHHHH
PELARAFLIRAQRQGLAARTFDKSALDLMAAYDWPGNVRELENLVLRLAVLSSDSVITVM
HHHHHHHHHHHHHCCCCHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCEEEEE
DVEREMRMGVAEQTGASLGFEAEIEGLLHRYVMGDLVSGGEGEGSNVHESVVNRVERPLI
ECHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
RLALSVTSGNKVRAAQLLGMNRNTLRAKINALGIAED
HHEEEECCCCCEEHHHHHCCCCHHHHEEHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]