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The map label for this gene is pgk [H]

Identifier: 114799328

GI number: 114799328

Start: 3356687

End: 3357883

Strand: Direct

Name: pgk [H]

Synonym: HNE_3180

Alternate gene names: 114799328

Gene position: 3356687-3357883 (Clockwise)

Preceding gene: 114798520

Following gene: 114797979

Centisome position: 90.6

GC content: 63.24

Gene sequence:

>1197_bases
ATGCCCTCGTTCAAACGCATCCCCGATGCAGGAAACCTCACCGGCAAGGTCGCGCTCGTCCGCGTTGACTTCAATGTGCC
GATGGAAAACGGTCTGGTGACGGATGATACGCGCCTGCGCGCCGCCGTCGATACCGTCCAGTCGCTGCGCACGGCCGGCG
CCAAGGTCGCCCTGCTGGCCCACTTTGGCCGCCCCAAGGGCGAGCGCGTCGCCGAGATGAGCCTCGCCCCGATTGCCGGC
GCCTTTGCCAAAGTGCTCGGCGCAAATGTCAGCTTCGTTGCCGACTGCAAGGGCGATCATGTGAAATCGGCCATTGCGGA
CCTTCTGCCCGCCGGCGTGATCCTTCTGGAAAACACGCGCTTCTACAAAGGCGAGGAAAAAGGCGACATGGAGCTGGCAA
AGGAAATCGCCGCTCTGGGCGACCTCTATGTCAACGACGCCTTCTCCGCCGCCCACCGCCGCCATGTCTCGACCGCTGTT
CTGGCGGATCTTCTGCCCGCCTATGCCGGCGTGACGATGGAAGCCGAACTCGATGCGCTCGACAAGGCGCTGGGCGCGCC
GGAGCGTCCGGTCATGGCCATCGTCGGCGGGGCAAAGGTCTCCTCCAAGATCGACCTTCTGAAAAACCTCGTCTCCAAGG
TCGACATGCTCGCGATTGGCGGGGGCATGGCCAACACGTTTCTCGCCGCAGACGGCGTGAATGTCGGCAAGTCGCTCTGC
GAGCATGATCTTGCCCCGACCGTCCGGGAAATCCAGGCGGCGGCCAAGGCGGCGGGCTGCGAAATCCTTCTGCCGAAGGA
CGTTGTCGTGGCGAAGGAATTCAAAGCCAATGCCGAAAACCGCACCTGCGGGCTGAATGAAATCGGCGCCGACGAAATGG
TGCTCGACGCGGGCGCGGCGACCATAACCTACCTCTCCGAAGCGCTTGGCCGCGCCAAGACCGTGATCTGGAACGGCCCT
CTCGGCGCCTTCGAGATGACGCCTTTTGACACCGCAACGGTGAAAGTCGCCGAAACAGCCGCCAAACTCGCCAAGTCCGG
CGCGCTGGTTGCCGTGGCAGGCGGAGGCGATACGGTGGCCGCGCTTAACCATGCCGGCGTCGCCCAGGATTTCACCTTCA
TCTCCACCGCAGGCGGAGCTTTCCTGGAATGGATGGAAGGCAAGGAACTGCCAGGCGTTGAAGCCTTGCTGAAATAG

Upstream 100 bases:

>100_bases
CGCCGCTCGCTCCTGCGCGATGGCGGGGGCCCCAGCGGCGGCATTTCATCAGACCTCACGCGCCCTCCGCATTCGCGCCG
GGGGACACCGGAGAATGACT

Downstream 100 bases:

>100_bases
AGCGGCCCACGCGGCGCAGATTTTGCATTGAGCCGCCACAAAGCGGACAAAAATCCGAGCCAGTAGCCAACACACGAACG
AACGTACCGGGACACAAAAC

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MPSFKRIPDAGNLTGKVALVRVDFNVPMENGLVTDDTRLRAAVDTVQSLRTAGAKVALLAHFGRPKGERVAEMSLAPIAG
AFAKVLGANVSFVADCKGDHVKSAIADLLPAGVILLENTRFYKGEEKGDMELAKEIAALGDLYVNDAFSAAHRRHVSTAV
LADLLPAYAGVTMEAELDALDKALGAPERPVMAIVGGAKVSSKIDLLKNLVSKVDMLAIGGGMANTFLAADGVNVGKSLC
EHDLAPTVREIQAAAKAAGCEILLPKDVVVAKEFKANAENRTCGLNEIGADEMVLDAGAATITYLSEALGRAKTVIWNGP
LGAFEMTPFDTATVKVAETAAKLAKSGALVAVAGGGDTVAALNHAGVAQDFTFISTAGGAFLEWMEGKELPGVEALLK

Sequences:

>Translated_398_residues
MPSFKRIPDAGNLTGKVALVRVDFNVPMENGLVTDDTRLRAAVDTVQSLRTAGAKVALLAHFGRPKGERVAEMSLAPIAG
AFAKVLGANVSFVADCKGDHVKSAIADLLPAGVILLENTRFYKGEEKGDMELAKEIAALGDLYVNDAFSAAHRRHVSTAV
LADLLPAYAGVTMEAELDALDKALGAPERPVMAIVGGAKVSSKIDLLKNLVSKVDMLAIGGGMANTFLAADGVNVGKSLC
EHDLAPTVREIQAAAKAAGCEILLPKDVVVAKEFKANAENRTCGLNEIGADEMVLDAGAATITYLSEALGRAKTVIWNGP
LGAFEMTPFDTATVKVAETAAKLAKSGALVAVAGGGDTVAALNHAGVAQDFTFISTAGGAFLEWMEGKELPGVEALLK
>Mature_397_residues
PSFKRIPDAGNLTGKVALVRVDFNVPMENGLVTDDTRLRAAVDTVQSLRTAGAKVALLAHFGRPKGERVAEMSLAPIAGA
FAKVLGANVSFVADCKGDHVKSAIADLLPAGVILLENTRFYKGEEKGDMELAKEIAALGDLYVNDAFSAAHRRHVSTAVL
ADLLPAYAGVTMEAELDALDKALGAPERPVMAIVGGAKVSSKIDLLKNLVSKVDMLAIGGGMANTFLAADGVNVGKSLCE
HDLAPTVREIQAAAKAAGCEILLPKDVVVAKEFKANAENRTCGLNEIGADEMVLDAGAATITYLSEALGRAKTVIWNGPL
GAFEMTPFDTATVKVAETAAKLAKSGALVAVAGGGDTVAALNHAGVAQDFTFISTAGGAFLEWMEGKELPGVEALLK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=407, Percent_Identity=40.2948402948403, Blast_Score=263, Evalue=2e-70,
Organism=Homo sapiens, GI31543397, Length=407, Percent_Identity=40.04914004914, Blast_Score=262, Evalue=4e-70,
Organism=Escherichia coli, GI1789294, Length=389, Percent_Identity=44.987146529563, Blast_Score=283, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17508823, Length=415, Percent_Identity=42.6506024096386, Blast_Score=292, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6319857, Length=402, Percent_Identity=39.5522388059701, Blast_Score=262, Evalue=9e-71,
Organism=Drosophila melanogaster, GI17136394, Length=404, Percent_Identity=38.6138613861386, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24581203, Length=408, Percent_Identity=37.7450980392157, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41265; Mature: 41134

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSFKRIPDAGNLTGKVALVRVDFNVPMENGLVTDDTRLRAAVDTVQSLRTAGAKVALLA
CCCCCCCCCCCCCCEEEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHCCCCEEEEE
HFGRPKGERVAEMSLAPIAGAFAKVLGANVSFVADCKGDHVKSAIADLLPAGVILLENTR
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCE
FYKGEEKGDMELAKEIAALGDLYVNDAFSAAHRRHVSTAVLADLLPAYAGVTMEAELDAL
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHH
DKALGAPERPVMAIVGGAKVSSKIDLLKNLVSKVDMLAIGGGMANTFLAADGVNVGKSLC
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHEEECCCCHHHHHHH
EHDLAPTVREIQAAAKAAGCEILLPKDVVVAKEFKANAENRTCGLNEIGADEMVLDAGAA
HHCCCHHHHHHHHHHHHCCCEEECCCCEEEHHHHHCCCCCCCCCCCCCCCCCEEEECCHH
TITYLSEALGRAKTVIWNGPLGAFEMTPFDTATVKVAETAAKLAKSGALVAVAGGGDTVA
HHHHHHHHHCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEE
ALNHAGVAQDFTFISTAGGAFLEWMEGKELPGVEALLK
HHHCCCCHHCCCEEECCCCHHHHHHCCCCCCCHHHHCC
>Mature Secondary Structure 
PSFKRIPDAGNLTGKVALVRVDFNVPMENGLVTDDTRLRAAVDTVQSLRTAGAKVALLA
CCCCCCCCCCCCCEEEEEEEEEECCCCCCCEECCCHHHHHHHHHHHHHHHCCCCEEEEE
HFGRPKGERVAEMSLAPIAGAFAKVLGANVSFVADCKGDHVKSAIADLLPAGVILLENTR
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCEEEEECCE
FYKGEEKGDMELAKEIAALGDLYVNDAFSAAHRRHVSTAVLADLLPAYAGVTMEAELDAL
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHH
DKALGAPERPVMAIVGGAKVSSKIDLLKNLVSKVDMLAIGGGMANTFLAADGVNVGKSLC
HHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCHHHHEEECCCCHHHHHHH
EHDLAPTVREIQAAAKAAGCEILLPKDVVVAKEFKANAENRTCGLNEIGADEMVLDAGAA
HHCCCHHHHHHHHHHHHCCCEEECCCCEEEHHHHHCCCCCCCCCCCCCCCCCEEEECCHH
TITYLSEALGRAKTVIWNGPLGAFEMTPFDTATVKVAETAAKLAKSGALVAVAGGGDTVA
HHHHHHHHHCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCEEE
ALNHAGVAQDFTFISTAGGAFLEWMEGKELPGVEALLK
HHHCCCCHHCCCEEECCCCHHHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2832389; 3680173 [H]