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The map label for this gene is ribH [H]

Identifier: 114799272

GI number: 114799272

Start: 2130088

End: 2130516

Strand: Reverse

Name: ribH [H]

Synonym: HNE_2055

Alternate gene names: 114799272

Gene position: 2130516-2130088 (Counterclockwise)

Preceding gene: 114799819

Following gene: 114800202

Centisome position: 57.5

GC content: 61.77

Gene sequence:

>429_bases
ATGGCTGACCGCGTTTTGATCGCGATTTCGCGCTATTACAAACACATCAGCGACGAGCTGGAAGCAGGCGCGCTCGAAGT
GCTCGAAGCTGCCGGCGCCAAGATCACCGTCATGGAAGTTCCCGGCGCTTTCGAAATCCCCGGCGTGATTGCCATGTCGG
CCGATTCCGGCCGGTTTGACGGCGCCGTCGCGCTTGGCTGCGTCATCCGCGGCGAGACGAGCCATTATGATTATGTCTGC
GGCGAGAGCGCACGCGGCCTGATGGACCTCATCGTCCAGCGCCGCCAGGCCATCGGCTACGGCATCCTCACGGTTGAAAA
CGAAGCGCAGGCGCTCGCCCGCGCGGATCGCAAACGCAAGAACAAGGGCGCCGAAGCGGCCAATGCCTGCCTTGCGATGA
TCAAATTCCGCAAAGAGCTGATCGGTTGA

Upstream 100 bases:

>100_bases
ATTTACCTGTCCGATACGCAGCCCACCCGCCTTGCGGGGCTTGACGGATACGGTTTGACAATAGAGGGCTGGCGCCACCT
GAGCGAGGAGAATACTTAAG

Downstream 100 bases:

>100_bases
TGACCCAGGAAATGCCCTTCGACGTCACACGCGCGCGCCGCGCTGGCGCGCGCCTTGCGGCGGTTCAGGCACTCTACGAA
ATGGAGCAGACGGAGAAATC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain [H]

Number of amino acids: Translated: 142; Mature: 141

Protein sequence:

>142_residues
MADRVLIAISRYYKHISDELEAGALEVLEAAGAKITVMEVPGAFEIPGVIAMSADSGRFDGAVALGCVIRGETSHYDYVC
GESARGLMDLIVQRRQAIGYGILTVENEAQALARADRKRKNKGAEAANACLAMIKFRKELIG

Sequences:

>Translated_142_residues
MADRVLIAISRYYKHISDELEAGALEVLEAAGAKITVMEVPGAFEIPGVIAMSADSGRFDGAVALGCVIRGETSHYDYVC
GESARGLMDLIVQRRQAIGYGILTVENEAQALARADRKRKNKGAEAANACLAMIKFRKELIG
>Mature_141_residues
ADRVLIAISRYYKHISDELEAGALEVLEAAGAKITVMEVPGAFEIPGVIAMSADSGRFDGAVALGCVIRGETSHYDYVCG
ESARGLMDLIVQRRQAIGYGILTVENEAQALARADRKRKNKGAEAANACLAMIKFRKELIG

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=141, Percent_Identity=41.1347517730496, Blast_Score=105, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324429, Length=149, Percent_Identity=30.8724832214765, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 15260; Mature: 15129

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRVLIAISRYYKHISDELEAGALEVLEAAGAKITVMEVPGAFEIPGVIAMSADSGRFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCCCC
GAVALGCVIRGETSHYDYVCGESARGLMDLIVQRRQAIGYGILTVENEAQALARADRKRK
CEEEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
NKGAEAANACLAMIKFRKELIG
CCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADRVLIAISRYYKHISDELEAGALEVLEAAGAKITVMEVPGAFEIPGVIAMSADSGRFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEECCCCCCCC
GAVALGCVIRGETSHYDYVCGESARGLMDLIVQRRQAIGYGILTVENEAQALARADRKRK
CEEEEEEEEECCCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
NKGAEAANACLAMIKFRKELIG
CCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA