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The map label for this gene is argG

Identifier: 114799175

GI number: 114799175

Start: 3625430

End: 3626671

Strand: Direct

Name: argG

Synonym: HNE_3491

Alternate gene names: 114799175

Gene position: 3625430-3626671 (Clockwise)

Preceding gene: 114799952

Following gene: 114799305

Centisome position: 97.85

GC content: 61.92

Gene sequence:

>1242_bases
ATGTCCAACGCTGCTTCCGCCCCGAAAAAGGTCGTTCTCGCCTATTCCGGAGGTCTCGACACCTCGATCATCCTCAAATG
GCTGCAGGTTGAGTTTGGCTGTGAGGTTGTCACCTTCACCGCCGACCTTGGCCAGGGCGAGGAACTGGAACCGGCCCGCA
AGAAGGCGCTGGCCGCCGGGGTGAAGCCGGAAAACATCTTCATCGAGGATGTGCGCGAGGAATTCGTGCGCGATTTCGTC
TTCCCGATGTTCCGCGCCAACGCCGTGTATGAAGGCGTCTATCTGCTGGGCACCTCGATTGCCCGGCCGCTGATCGCCAA
GCGCCAGATCGAGATTGCCGACATGGTCGGCGCGGACGCAGTCTGTCATGGCGCCACCGGCAAGGGGAATGATCAGGTCC
GGTTTGAGCTGGGCTATTATGGCCTTAATCCGGACATCAAGGTGATCGCGCCGTGGCGCGACTGGGCGTTCAAATCCCGG
ACCGACCTTTTGAAGTTCGCCGAGGAACACGGCATCGAGATCGCCAAGGACAAGCGCGGCGAAGCGCCGTTCTCGGTGGA
CGCTAACCTGCTGCACTCTTCCTCCGAGGGCAAAGTGCTGGAAAACCCGGCCGAGCCTGCGCCGGAATTCGTGCACATGC
GCACGGTCAGCCCGGAAGATGCGCCTGACCAGGCCGAGGTGATCACCATCAGCTTTGAAAAAGGCGACGCGGTGGCGATC
AATGGCGAGGCGATGAGCCCGGCGACGCTGCTCACCGCGCTCAACGCTTACGGCAAGAAGCACGGCATTGGCCGGCTCGA
CTTGTTGGAAAACCGCTTCGTCGGCATGAAGTCGCGCGGTATTTACGAGACGCCGGGCGGGACGATCCTGCTGGTGGCCC
ACCGGGGCATCGAGCAGGCGACGCTCGACCGGGGCGCGGCCCACCTTAAAGACGAGCTGATGCCGAAATATGCCGAGCTG
ATCTATAACGGCTTCTGGTGGAGCCCGGAGCGCGAGATGCTGCAGGCGGCCATCGACCATTCGCAGCGCTGGGTGACCGG
CGATGTGACGATGAAGCTCTACAAGGGCAACGCCTATCTGATCGGCCGTTCGTCTCCCTACAGTCTGTACTCGGAGAAGA
TCGTGACCTTCGAGGACGACCACGGCGCTTACGACCAGAAGGATGCGGCCGGCTTCATCAAGCTCAACGCGCTGCGTTTG
CGCCTTCTGGCGGGCCGCGACCGGAAGTTCGGCAAGAACTGA

Upstream 100 bases:

>100_bases
ACCATAAAATTCCCTGTAAGCCGCCGCCTTGTCAGGGCGGGTGCGGGACTTTAAGGCAACAGGTCTCCGTTTCCGCCCCA
GAAAGACGACAGAAAATCCC

Downstream 100 bases:

>100_bases
TCGCTTTCACGCCAACACAAACACCGGCGCCCTCCCCTGACCTTCAGGGGAGGCGCTTTTTTATGGTCCGGCGTTCAGCA
TCAGGACGTGTTGATCCCGG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSNAASAPKKVVLAYSGGLDTSIILKWLQVEFGCEVVTFTADLGQGEELEPARKKALAAGVKPENIFIEDVREEFVRDFV
FPMFRANAVYEGVYLLGTSIARPLIAKRQIEIADMVGADAVCHGATGKGNDQVRFELGYYGLNPDIKVIAPWRDWAFKSR
TDLLKFAEEHGIEIAKDKRGEAPFSVDANLLHSSSEGKVLENPAEPAPEFVHMRTVSPEDAPDQAEVITISFEKGDAVAI
NGEAMSPATLLTALNAYGKKHGIGRLDLLENRFVGMKSRGIYETPGGTILLVAHRGIEQATLDRGAAHLKDELMPKYAEL
IYNGFWWSPEREMLQAAIDHSQRWVTGDVTMKLYKGNAYLIGRSSPYSLYSEKIVTFEDDHGAYDQKDAAGFIKLNALRL
RLLAGRDRKFGKN

Sequences:

>Translated_413_residues
MSNAASAPKKVVLAYSGGLDTSIILKWLQVEFGCEVVTFTADLGQGEELEPARKKALAAGVKPENIFIEDVREEFVRDFV
FPMFRANAVYEGVYLLGTSIARPLIAKRQIEIADMVGADAVCHGATGKGNDQVRFELGYYGLNPDIKVIAPWRDWAFKSR
TDLLKFAEEHGIEIAKDKRGEAPFSVDANLLHSSSEGKVLENPAEPAPEFVHMRTVSPEDAPDQAEVITISFEKGDAVAI
NGEAMSPATLLTALNAYGKKHGIGRLDLLENRFVGMKSRGIYETPGGTILLVAHRGIEQATLDRGAAHLKDELMPKYAEL
IYNGFWWSPEREMLQAAIDHSQRWVTGDVTMKLYKGNAYLIGRSSPYSLYSEKIVTFEDDHGAYDQKDAAGFIKLNALRL
RLLAGRDRKFGKN
>Mature_412_residues
SNAASAPKKVVLAYSGGLDTSIILKWLQVEFGCEVVTFTADLGQGEELEPARKKALAAGVKPENIFIEDVREEFVRDFVF
PMFRANAVYEGVYLLGTSIARPLIAKRQIEIADMVGADAVCHGATGKGNDQVRFELGYYGLNPDIKVIAPWRDWAFKSRT
DLLKFAEEHGIEIAKDKRGEAPFSVDANLLHSSSEGKVLENPAEPAPEFVHMRTVSPEDAPDQAEVITISFEKGDAVAIN
GEAMSPATLLTALNAYGKKHGIGRLDLLENRFVGMKSRGIYETPGGTILLVAHRGIEQATLDRGAAHLKDELMPKYAELI
YNGFWWSPEREMLQAAIDHSQRWVTGDVTMKLYKGNAYLIGRSSPYSLYSEKIVTFEDDHGAYDQKDAAGFIKLNALRLR
LLAGRDRKFGKN

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=405, Percent_Identity=49.6296296296296, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI16950633, Length=405, Percent_Identity=49.6296296296296, Blast_Score=368, Evalue=1e-102,
Organism=Escherichia coli, GI1789563, Length=427, Percent_Identity=29.5081967213115, Blast_Score=137, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324514, Length=406, Percent_Identity=42.8571428571429, Blast_Score=320, Evalue=2e-88,
Organism=Drosophila melanogaster, GI21358151, Length=408, Percent_Identity=45.343137254902, Blast_Score=335, Evalue=4e-92,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_HYPNA (Q0BWI1)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_762162.1
- ProteinModelPortal:   Q0BWI1
- SMR:   Q0BWI1
- STRING:   Q0BWI1
- GeneID:   4288392
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3491
- NMPDR:   fig|228405.5.peg.3343
- TIGR:   HNE_3491
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   PLIAKRQ
- PhylomeDB:   Q0BWI1
- ProtClustDB:   PRK00509
- BioCyc:   HNEP81032:HNE_3491-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45657; Mature: 45525

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 41-41 BINDING 94-94 BINDING 99-99 BINDING 124-124 BINDING 126-126 BINDING 130-130 BINDING 130-130 BINDING 131-131 BINDING 134-134 BINDING 185-185 BINDING 194-194 BINDING 270-270 BINDING 282-282

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAASAPKKVVLAYSGGLDTSIILKWLQVEFGCEVVTFTADLGQGEELEPARKKALAAG
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCC
VKPENIFIEDVREEFVRDFVFPMFRANAVYEGVYLLGTSIARPLIAKRQIEIADMVGADA
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHEEHHHHHHHHHHHHCCHHHHHCCCCC
VCHGATGKGNDQVRFELGYYGLNPDIKVIAPWRDWAFKSRTDLLKFAEEHGIEIAKDKRG
EEECCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCC
EAPFSVDANLLHSSSEGKVLENPAEPAPEFVHMRTVSPEDAPDQAEVITISFEKGDAVAI
CCCCEECHHHEECCCCCCEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEE
NGEAMSPATLLTALNAYGKKHGIGRLDLLENRFVGMKSRGIYETPGGTILLVAHRGIEQA
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEECCCCEEEEEEECCCCHH
TLDRGAAHLKDELMPKYAELIYNGFWWSPEREMLQAAIDHSQRWVTGDVTMKLYKGNAYL
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEECEEEEEEECCEEE
IGRSSPYSLYSEKIVTFEDDHGAYDQKDAAGFIKLNALRLRLLAGRDRKFGKN
EECCCCCCHHHCEEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure 
SNAASAPKKVVLAYSGGLDTSIILKWLQVEFGCEVVTFTADLGQGEELEPARKKALAAG
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHCC
VKPENIFIEDVREEFVRDFVFPMFRANAVYEGVYLLGTSIARPLIAKRQIEIADMVGADA
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCHHEEHHHHHHHHHHHHCCHHHHHCCCCC
VCHGATGKGNDQVRFELGYYGLNPDIKVIAPWRDWAFKSRTDLLKFAEEHGIEIAKDKRG
EEECCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCC
EAPFSVDANLLHSSSEGKVLENPAEPAPEFVHMRTVSPEDAPDQAEVITISFEKGDAVAI
CCCCEECHHHEECCCCCCEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCEEEE
NGEAMSPATLLTALNAYGKKHGIGRLDLLENRFVGMKSRGIYETPGGTILLVAHRGIEQA
CCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCEECCCCEEEEEEECCCCHH
TLDRGAAHLKDELMPKYAELIYNGFWWSPEREMLQAAIDHSQRWVTGDVTMKLYKGNAYL
HHHCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEECEEEEEEECCEEE
IGRSSPYSLYSEKIVTFEDDHGAYDQKDAAGFIKLNALRLRLLAGRDRKFGKN
EECCCCCCHHHCEEEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA