Click here to switch to the map view.

The map label for this gene is proB [H]

Identifier: 114799163

GI number: 114799163

Start: 2720158

End: 2721273

Strand: Direct

Name: proB [H]

Synonym: HNE_2567

Alternate gene names: 114799163

Gene position: 2720158-2721273 (Clockwise)

Preceding gene: 114799947

Following gene: 114800022

Centisome position: 73.42

GC content: 71.59

Gene sequence:

>1116_bases
ATGACTGCTGACACCCTCTCCCGCGCCAAGCGCATCGTCGTGAAAACCGGCTCTGCCCTCATCGCGGAGGCAGGCGCTCC
GCGCCGCGAATGGCTCGCCGCCCTCGCCGCAGACATCGCCGCCCTGCGCCAGCGCGGCAAGGAAGTCATCCTCGTCTCCT
CCGGCGCCGTTGCCCTTGGCCGCTCAGCCCTCGGCGCGCCCTATCAAGGCCGCCTGGAACAGAAACAGGCCGCCGCCGCC
ATCGGCCAGCCCCGGCTCATGGCCGCGCTGGGAGACGCCCTCGCCCCCCACGCCATCGCCTGCGGCCAGGCCCTCCTCAC
CCCCGGCGACACCGAAAATCGCCGCAGATGGCTGAATGCCCGCGCCACGCTGGAAACCCTGCTGGAAGCCGGTGTCGTCC
CCGTCATCAACGAGAACGATACCGTCGCCACCGAAGAAATCCGCTACGGGGATAATGACCGCCTCGCCGCCCGCGTCGCC
CAGATGATGGCCGCTGATGTCCTGATCCTCCTCTCGGACATCGACGGCCTCTACACCGCAGACCCGCGCTCAAACCCCGG
CGCCCCCCACATCCCCTTCCTCGAAGCGCTCACCCCTGAGCATGACGCCATGGCCACCGGCGCCAACGCCGCCGCCGGTG
TCGGCTCCGGCGGCATGGCCACCAAACTCGCCGCCGCCCGCATCGCCCATGCGGCGGGCTGCTCCACCCTCATCACGCTC
GGCAACCGCCCCCACCCCCTCGGCGCCATCGAGGCCGGCGAACGCGCCACCCTCATCGCCTCCCGCATCAGCCCCGCCAA
GGCCCGCGCCGCCTGGCTCGAAGGCCACCTCAAGCCCGAAGGCGAACTCCTCATCGATGACGGCGCCGCCGCCGCGCTGG
CAAAAGGCGCGAGCCTCCTCGCCGTCGGCGTGCGCGGCGTCACCGGCCAGTTCGAGCGCGGCGCCGCCGTCGCCATCCGC
GCCCCCGATGGCCGCATCATAGCCAAGGGCGTCACCGCCTACGGCACCGAAGACATCCGCCGCATCGCCGGCCGCCGCAC
CGAAGACGCCGAAGCCATCCTCGGCTATCGCGGCCGCCCCGCCATCATCCACCGCGACGACATGGTGCGCCTTTGA

Upstream 100 bases:

>100_bases
AATACGCCCCGAAATGCCCTTCCGGCTCGCCGCCGGGAGGGCATTTCGCCGTTTCATAACCTCTCGCCATGGCGTCTCTG
GCCGTGTATCGCGGTCAGCC

Downstream 100 bases:

>100_bases
CTGAGCCGCTGATCCGGCCCGCCACGCAGGCTGACATCGGCGCGCTCACCGCCCTGGAAGCCAGCTTCCCCGAAGAAGAC
CGCTTCGACCGGCGCACCTG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTADTLSRAKRIVVKTGSALIAEAGAPRREWLAALAADIAALRQRGKEVILVSSGAVALGRSALGAPYQGRLEQKQAAAA
IGQPRLMAALGDALAPHAIACGQALLTPGDTENRRRWLNARATLETLLEAGVVPVINENDTVATEEIRYGDNDRLAARVA
QMMAADVLILLSDIDGLYTADPRSNPGAPHIPFLEALTPEHDAMATGANAAAGVGSGGMATKLAAARIAHAAGCSTLITL
GNRPHPLGAIEAGERATLIASRISPAKARAAWLEGHLKPEGELLIDDGAAAALAKGASLLAVGVRGVTGQFERGAAVAIR
APDGRIIAKGVTAYGTEDIRRIAGRRTEDAEAILGYRGRPAIIHRDDMVRL

Sequences:

>Translated_371_residues
MTADTLSRAKRIVVKTGSALIAEAGAPRREWLAALAADIAALRQRGKEVILVSSGAVALGRSALGAPYQGRLEQKQAAAA
IGQPRLMAALGDALAPHAIACGQALLTPGDTENRRRWLNARATLETLLEAGVVPVINENDTVATEEIRYGDNDRLAARVA
QMMAADVLILLSDIDGLYTADPRSNPGAPHIPFLEALTPEHDAMATGANAAAGVGSGGMATKLAAARIAHAAGCSTLITL
GNRPHPLGAIEAGERATLIASRISPAKARAAWLEGHLKPEGELLIDDGAAAALAKGASLLAVGVRGVTGQFERGAAVAIR
APDGRIIAKGVTAYGTEDIRRIAGRRTEDAEAILGYRGRPAIIHRDDMVRL
>Mature_370_residues
TADTLSRAKRIVVKTGSALIAEAGAPRREWLAALAADIAALRQRGKEVILVSSGAVALGRSALGAPYQGRLEQKQAAAAI
GQPRLMAALGDALAPHAIACGQALLTPGDTENRRRWLNARATLETLLEAGVVPVINENDTVATEEIRYGDNDRLAARVAQ
MMAADVLILLSDIDGLYTADPRSNPGAPHIPFLEALTPEHDAMATGANAAAGVGSGGMATKLAAARIAHAAGCSTLITLG
NRPHPLGAIEAGERATLIASRISPAKARAAWLEGHLKPEGELLIDDGAAAALAKGASLLAVGVRGVTGQFERGAAVAIRA
PDGRIIAKGVTAYGTEDIRRIAGRRTEDAEAILGYRGRPAIIHRDDMVRL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=299, Percent_Identity=32.4414715719064, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI62912457, Length=297, Percent_Identity=32.6599326599327, Blast_Score=121, Evalue=1e-27,
Organism=Escherichia coli, GI1786437, Length=370, Percent_Identity=42.972972972973, Blast_Score=264, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI17569969, Length=262, Percent_Identity=34.3511450381679, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17569967, Length=262, Percent_Identity=34.3511450381679, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=421, Percent_Identity=31.8289786223278, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321822, Length=421, Percent_Identity=30.4038004750594, Blast_Score=176, Evalue=6e-45,
Organism=Drosophila melanogaster, GI21357643, Length=284, Percent_Identity=30.9859154929577, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 38458; Mature: 38326

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADTLSRAKRIVVKTGSALIAEAGAPRREWLAALAADIAALRQRGKEVILVSSGAVALG
CCHHHHHHHHEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHC
RSALGAPYQGRLEQKQAAAAIGQPRLMAALGDALAPHAIACGQALLTPGDTENRRRWLNA
HHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCHHCCCCCCHHHHHHHHH
RATLETLLEAGVVPVINENDTVATEEIRYGDNDRLAARVAQMMAADVLILLSDIDGLYTA
HHHHHHHHHCCCEEEECCCCCEEHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEC
DPRSNPGAPHIPFLEALTPEHDAMATGANAAAGVGSGGMATKLAAARIAHAAGCSTLITL
CCCCCCCCCCCCHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
GNRPHPLGAIEAGERATLIASRISPAKARAAWLEGHLKPEGELLIDDGAAAALAKGASLL
CCCCCCCCCCCCCCCCEEHHHHCCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCCEEE
AVGVRGVTGQFERGAAVAIRAPDGRIIAKGVTAYGTEDIRRIAGRRTEDAEAILGYRGRP
EEECCCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCC
AIIHRDDMVRL
EEEECCCCCCC
>Mature Secondary Structure 
TADTLSRAKRIVVKTGSALIAEAGAPRREWLAALAADIAALRQRGKEVILVSSGAVALG
CHHHHHHHHEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHC
RSALGAPYQGRLEQKQAAAAIGQPRLMAALGDALAPHAIACGQALLTPGDTENRRRWLNA
HHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHCCCHHCCCCCCHHHHHHHHH
RATLETLLEAGVVPVINENDTVATEEIRYGDNDRLAARVAQMMAADVLILLSDIDGLYTA
HHHHHHHHHCCCEEEECCCCCEEHHHEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEC
DPRSNPGAPHIPFLEALTPEHDAMATGANAAAGVGSGGMATKLAAARIAHAAGCSTLITL
CCCCCCCCCCCCHHHHCCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
GNRPHPLGAIEAGERATLIASRISPAKARAAWLEGHLKPEGELLIDDGAAAALAKGASLL
CCCCCCCCCCCCCCCCEEHHHHCCHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCCEEE
AVGVRGVTGQFERGAAVAIRAPDGRIIAKGVTAYGTEDIRRIAGRRTEDAEAILGYRGRP
EEECCCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCC
AIIHRDDMVRL
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA