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The map label for this gene is pldB [H]

Identifier: 114799139

GI number: 114799139

Start: 86433

End: 87386

Strand: Direct

Name: pldB [H]

Synonym: HNE_0098

Alternate gene names: 114799139

Gene position: 86433-87386 (Clockwise)

Preceding gene: 114797960

Following gene: 114797267

Centisome position: 2.33

GC content: 60.17

Gene sequence:

>954_bases
ATGGCGAAATCCTCCGATGACAGGCGGAGCCGGTTTGTTGAGGTTCCGTGGAACACGCCGCCCAAAGGCGCCGAAATCGT
ATGGTTTGAGGGGTCTGAAGGGCGCCGTCTCCGGGCCTGCATCGCGCCGGCGCTCTCTCAGTCCAAGCCGCGGGGCACAG
TTATCGTCTGTCCGGGGCGCACAGAGTTCATCGAAAAATATTTCGAAGTGGGCCGTGAGTTGCAGCAAATGGGCTTTGCG
CTCGTGATCCTGGACTGGCCGGGCCAGGGTCTCTCGGACCGCCTTCTGCCTGACAGCAAGAAGGGTCATATCGACCGGTT
CGAGACCTTCATGACAGCCCTCGCCAAGGGGCTGGAGGCGTTCGACAGCCATTTGCCACGCCCATATATTTCGCTGGCCC
ACTCGATGGGAGGCGCCATTGCGCTGGCTGCTATTGCCAAAGGTCTTGTGAAGGTGGAGGCGGCCGCCTTCTGCGCGCCG
ATGTGGGGTATTAAATCGCCGGTCCTTGGCATGCGATACCTCGTCTGGGCCATGCGGGCGACAGGGCGCTCAGGCGACTA
TGCGATGCAGCCCGGCCCGCCGGAACGGTTTGAAACCAATATCGTGACCCATGACAAGCGCCGCTGGGACTTGCAGCGCG
CCCTCATTGATGCCCAGCCCGATCTGGAACTCGGCCCGGTCACGTGGGGCTGGTTGGGCGCCTCTCTGGATATTTTCTCG
GCCTTTTCAAAACCGAAGGCGCTGGCAAGCATCGACATTCCGGTCTTTGTGGCATCCGCCGGAGAAGAAAAGCTCGTCGA
TAATGCTTCCCATGCCAAAATCGCGGCTCGTCTGAAGGATTGCGATCACATCACGGTTGACGGCGCGATGCACGAAATCC
TGATGGAGACAGACGACCGGCGCGCCGAGTTCTGGGACGGCTTTCAAAAATTACTCAAACGTGCGGGCATCTGA

Upstream 100 bases:

>100_bases
AGCTGGCTGGCTGAGCCATCTGAATCAGATCGCTTGACGCATTGGGCCGCTCCGGGGAGACCTGCGGGCGGCTCAATTTT
TGGCCCTGGGAGGCCTGTGC

Downstream 100 bases:

>100_bases
CCCAGCACGGATATAAAAACGGGAGGAAATACTTATGGCGATCATTTCACTGTCGCGCATCGTGGCCCACTGGGCTGCGC
AGCAGCCGTCACGCACAGTC

Product: alpha/beta fold family hydrolase

Products: NA

Alternate protein names: Lecithinase B [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MAKSSDDRRSRFVEVPWNTPPKGAEIVWFEGSEGRRLRACIAPALSQSKPRGTVIVCPGRTEFIEKYFEVGRELQQMGFA
LVILDWPGQGLSDRLLPDSKKGHIDRFETFMTALAKGLEAFDSHLPRPYISLAHSMGGAIALAAIAKGLVKVEAAAFCAP
MWGIKSPVLGMRYLVWAMRATGRSGDYAMQPGPPERFETNIVTHDKRRWDLQRALIDAQPDLELGPVTWGWLGASLDIFS
AFSKPKALASIDIPVFVASAGEEKLVDNASHAKIAARLKDCDHITVDGAMHEILMETDDRRAEFWDGFQKLLKRAGI

Sequences:

>Translated_317_residues
MAKSSDDRRSRFVEVPWNTPPKGAEIVWFEGSEGRRLRACIAPALSQSKPRGTVIVCPGRTEFIEKYFEVGRELQQMGFA
LVILDWPGQGLSDRLLPDSKKGHIDRFETFMTALAKGLEAFDSHLPRPYISLAHSMGGAIALAAIAKGLVKVEAAAFCAP
MWGIKSPVLGMRYLVWAMRATGRSGDYAMQPGPPERFETNIVTHDKRRWDLQRALIDAQPDLELGPVTWGWLGASLDIFS
AFSKPKALASIDIPVFVASAGEEKLVDNASHAKIAARLKDCDHITVDGAMHEILMETDDRRAEFWDGFQKLLKRAGI
>Mature_316_residues
AKSSDDRRSRFVEVPWNTPPKGAEIVWFEGSEGRRLRACIAPALSQSKPRGTVIVCPGRTEFIEKYFEVGRELQQMGFAL
VILDWPGQGLSDRLLPDSKKGHIDRFETFMTALAKGLEAFDSHLPRPYISLAHSMGGAIALAAIAKGLVKVEAAAFCAPM
WGIKSPVLGMRYLVWAMRATGRSGDYAMQPGPPERFETNIVTHDKRRWDLQRALIDAQPDLELGPVTWGWLGASLDIFSA
FSKPKALASIDIPVFVASAGEEKLVDNASHAKIAARLKDCDHITVDGAMHEILMETDDRRAEFWDGFQKLLKRAGI

Specific function: Unknown

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI48994980, Length=264, Percent_Identity=32.5757575757576, Blast_Score=111, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 35105; Mature: 34974

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSSDDRRSRFVEVPWNTPPKGAEIVWFEGSEGRRLRACIAPALSQSKPRGTVIVCPGR
CCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCC
TEFIEKYFEVGRELQQMGFALVILDWPGQGLSDRLLPDSKKGHIDRFETFMTALAKGLEA
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FDSHLPRPYISLAHSMGGAIALAAIAKGLVKVEAAAFCAPMWGIKSPVLGMRYLVWAMRA
HHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TGRSGDYAMQPGPPERFETNIVTHDKRRWDLQRALIDAQPDLELGPVTWGWLGASLDIFS
CCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCEECCEEHHHHCCHHHHHH
AFSKPKALASIDIPVFVASAGEEKLVDNASHAKIAARLKDCDHITVDGAMHEILMETDDR
HHCCCCCEEEECCEEEEECCCCHHHHCCCHHHHHHHHHHCCCEEEECHHHHHHHHCCCCC
RAEFWDGFQKLLKRAGI
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKSSDDRRSRFVEVPWNTPPKGAEIVWFEGSEGRRLRACIAPALSQSKPRGTVIVCPGR
CCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCC
TEFIEKYFEVGRELQQMGFALVILDWPGQGLSDRLLPDSKKGHIDRFETFMTALAKGLEA
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
FDSHLPRPYISLAHSMGGAIALAAIAKGLVKVEAAAFCAPMWGIKSPVLGMRYLVWAMRA
HHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
TGRSGDYAMQPGPPERFETNIVTHDKRRWDLQRALIDAQPDLELGPVTWGWLGASLDIFS
CCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHCCCCCCEECCEEHHHHCCHHHHHH
AFSKPKALASIDIPVFVASAGEEKLVDNASHAKIAARLKDCDHITVDGAMHEILMETDDR
HHCCCCCEEEECCEEEEECCCCHHHHCCCHHHHHHHHHHCCCEEEECHHHHHHHHCCCCC
RAEFWDGFQKLLKRAGI
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]