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The map label for this gene is baiB [H]

Identifier: 114797267

GI number: 114797267

Start: 87421

End: 88878

Strand: Direct

Name: baiB [H]

Synonym: HNE_0099

Alternate gene names: 114797267

Gene position: 87421-88878 (Clockwise)

Preceding gene: 114799139

Following gene: 114797977

Centisome position: 2.36

GC content: 60.77

Gene sequence:

>1458_bases
ATGGCGATCATTTCACTGTCGCGCATCGTGGCCCACTGGGCTGCGCAGCAGCCGTCACGCACAGTCCTAAGCCATGAAGG
CAGGGATGTCAGCTGGGCCGAGTTTGAGGCCCGCACAAACCGCCTGGCGCGCGCCTATCAGAAACTCGGTGTGAAACAGG
ACGATTTCGTCACCATCTCACTGCCCAATGGCATAGAGTTTTTCGAGGCCTGCTTTGCGACCTGGAAAGCAGGCGCAACG
CCCCAGCCCATTTCCGCGCGCCTGCCCAAACTGGAGCGCGATCAGATCGTCGAGATCGGCGCGCCAAGCCTGGTGGTGGG
CGTTCCCGGCGGAGAGTATCCCCAGACGGCATGTGTCCCTCAAGGGTTTGAGCCAGACACCGGACTTTCAGATGCTCCGT
TGCCGGAAGTTACGGCCGCCAGCTACAAGGCCATGACATCGGGCGGCTCAACCGGGCGCCCGAAACTGATCGTTTCAAAA
CAGCCTGCTGCCGCAGACCCCGACGTGCCGATGCTGGAAATTCCCCAGCAGGGCTGCATGCTGATCCCCGGCCCACTCTA
TCACAACGGCCCCTTCCTCTGGGCGATGACGGCGCTCTTCAAAGGCTGCACTATTGTTGTCACCACACGGTTTGACGCTG
AAGAAACGCTGAAATTGATCGAAAAGCACAAGGCTGATGTGATCTACACCGTGCCAACCATGATGCGGCGCATCTGGTCG
CTGCCTGAAGAGGTCCGCGCATCGTATGACCTCTCCAGCCTGAAGGCGCTCTGGCACCTGGCCGCGCCATGTCCGGCATG
GCTTAAAGAATGCTTCATCGAATGGCTCGGCCCGGAGGTAGTCTGGGAACTTTACGGCGGCACGGAGGGGCAGGGCTCCA
CGACCATTCAGGGTACGGACTGGCTCAAACACAAGGGCTCGGTCGGCAAGCCGGTGGAAACCTGCGAAATGAAAATCGTC
GATGAAGATGGGAAAACCCTGCCGCCCCGCGAAGTGGGCGAAGTTTTCATACGCCCGCTCGCCGGTGCGGGCACGACATA
TCGTTATATCGGCGCCGATGCCAAAGCCATTGATGGCGGCTGGGAAAGCCTCGGCGATCTGGGCTGGATGGACGAGGACG
GATTTCTCTATCTGTCCGACCGTCTGTCAGACATGGTCATCGTTGGGGGAGCCAACATCTATCCGGCAGAGGTGGAGGCC
GCGATCGAGGCCTATCCGGGCGTTCGTTCGTCTGCTGTCATCGGTCTGCCGGACGAAGACATGGGCGCGCGCCTGCATGC
TGTGATCGACCGTCCAGAGGGGCCGGTCGACGATGCCACGATGATTGCCCATCTTTCCGAGCGGCTCGTGCGCTACAAGA
TCCCCAAGAGCTTCGAATATTCCGCTGAAGCCGTCCGGGATGACGCGGGGAAAGTGCGCCGCAAAGCCTTACGCGAGGCG
AGGCTCGCCGCCTCTTGA

Upstream 100 bases:

>100_bases
ACAGACGACCGGCGCGCCGAGTTCTGGGACGGCTTTCAAAAATTACTCAAACGTGCGGGCATCTGACCCAGCACGGATAT
AAAAACGGGAGGAAATACTT

Downstream 100 bases:

>100_bases
CTTCCCCAAGGGCGCTGCTCGTCCGGTCGCGCCACATCCAAGGGAGGATCAGATCATGAAAGTAGCAGCCGTCAAAAAGC
CCGGCGGGCTTCAGAACCTC

Product: bile acid-coenzyme A ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MAIISLSRIVAHWAAQQPSRTVLSHEGRDVSWAEFEARTNRLARAYQKLGVKQDDFVTISLPNGIEFFEACFATWKAGAT
PQPISARLPKLERDQIVEIGAPSLVVGVPGGEYPQTACVPQGFEPDTGLSDAPLPEVTAASYKAMTSGGSTGRPKLIVSK
QPAAADPDVPMLEIPQQGCMLIPGPLYHNGPFLWAMTALFKGCTIVVTTRFDAEETLKLIEKHKADVIYTVPTMMRRIWS
LPEEVRASYDLSSLKALWHLAAPCPAWLKECFIEWLGPEVVWELYGGTEGQGSTTIQGTDWLKHKGSVGKPVETCEMKIV
DEDGKTLPPREVGEVFIRPLAGAGTTYRYIGADAKAIDGGWESLGDLGWMDEDGFLYLSDRLSDMVIVGGANIYPAEVEA
AIEAYPGVRSSAVIGLPDEDMGARLHAVIDRPEGPVDDATMIAHLSERLVRYKIPKSFEYSAEAVRDDAGKVRRKALREA
RLAAS

Sequences:

>Translated_485_residues
MAIISLSRIVAHWAAQQPSRTVLSHEGRDVSWAEFEARTNRLARAYQKLGVKQDDFVTISLPNGIEFFEACFATWKAGAT
PQPISARLPKLERDQIVEIGAPSLVVGVPGGEYPQTACVPQGFEPDTGLSDAPLPEVTAASYKAMTSGGSTGRPKLIVSK
QPAAADPDVPMLEIPQQGCMLIPGPLYHNGPFLWAMTALFKGCTIVVTTRFDAEETLKLIEKHKADVIYTVPTMMRRIWS
LPEEVRASYDLSSLKALWHLAAPCPAWLKECFIEWLGPEVVWELYGGTEGQGSTTIQGTDWLKHKGSVGKPVETCEMKIV
DEDGKTLPPREVGEVFIRPLAGAGTTYRYIGADAKAIDGGWESLGDLGWMDEDGFLYLSDRLSDMVIVGGANIYPAEVEA
AIEAYPGVRSSAVIGLPDEDMGARLHAVIDRPEGPVDDATMIAHLSERLVRYKIPKSFEYSAEAVRDDAGKVRRKALREA
RLAAS
>Mature_484_residues
AIISLSRIVAHWAAQQPSRTVLSHEGRDVSWAEFEARTNRLARAYQKLGVKQDDFVTISLPNGIEFFEACFATWKAGATP
QPISARLPKLERDQIVEIGAPSLVVGVPGGEYPQTACVPQGFEPDTGLSDAPLPEVTAASYKAMTSGGSTGRPKLIVSKQ
PAAADPDVPMLEIPQQGCMLIPGPLYHNGPFLWAMTALFKGCTIVVTTRFDAEETLKLIEKHKADVIYTVPTMMRRIWSL
PEEVRASYDLSSLKALWHLAAPCPAWLKECFIEWLGPEVVWELYGGTEGQGSTTIQGTDWLKHKGSVGKPVETCEMKIVD
EDGKTLPPREVGEVFIRPLAGAGTTYRYIGADAKAIDGGWESLGDLGWMDEDGFLYLSDRLSDMVIVGGANIYPAEVEAA
IEAYPGVRSSAVIGLPDEDMGARLHAVIDRPEGPVDDATMIAHLSERLVRYKIPKSFEYSAEAVRDDAGKVRRKALREAR
LAAS

Specific function: Active with a large number of C-24 bile acids possessing unhindered C-24 carboxyl groups. Requires ATP and produces AMP and pyrophosphate in addition to the bile-acid CoA thioester [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=521, Percent_Identity=25.9117082533589, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI42544132, Length=283, Percent_Identity=30.0353356890459, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI115511026, Length=533, Percent_Identity=24.390243902439, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI122937307, Length=236, Percent_Identity=30.9322033898305, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI38505220, Length=283, Percent_Identity=28.9752650176678, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI157311624, Length=299, Percent_Identity=26.7558528428094, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI157311622, Length=299, Percent_Identity=26.7558528428094, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI58082049, Length=308, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI187761345, Length=525, Percent_Identity=23.4285714285714, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI187761343, Length=525, Percent_Identity=23.4285714285714, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI28416953, Length=377, Percent_Identity=24.6684350132626, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788107, Length=544, Percent_Identity=25.9191176470588, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI221142682, Length=495, Percent_Identity=24.8484848484848, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI145693145, Length=503, Percent_Identity=24.8508946322068, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786810, Length=490, Percent_Identity=25.3061224489796, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1788595, Length=487, Percent_Identity=24.6406570841889, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790505, Length=278, Percent_Identity=25.1798561151079, Blast_Score=72, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17531443, Length=524, Percent_Identity=23.8549618320611, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=492, Percent_Identity=25.2032520325203, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=301, Percent_Identity=28.2392026578073, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=23.2931726907631, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=528, Percent_Identity=25.3787878787879, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=317, Percent_Identity=28.0757097791798, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=313, Percent_Identity=28.4345047923323, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71994694, Length=317, Percent_Identity=28.0757097791798, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=496, Percent_Identity=24.5967741935484, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=364, Percent_Identity=25.2747252747253, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=288, Percent_Identity=27.7777777777778, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI25141359, Length=481, Percent_Identity=23.4927234927235, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=306, Percent_Identity=23.8562091503268, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=314, Percent_Identity=24.8407643312102, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323182, Length=564, Percent_Identity=21.9858156028369, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355181, Length=501, Percent_Identity=26.7465069860279, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24656500, Length=425, Percent_Identity=24.7058823529412, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21358303, Length=460, Percent_Identity=23.2608695652174, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24648253, Length=452, Percent_Identity=23.4513274336283, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648255, Length=454, Percent_Identity=23.7885462555066, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI21356947, Length=523, Percent_Identity=24.282982791587, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18859661, Length=499, Percent_Identity=24.6492985971944, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922652, Length=432, Percent_Identity=23.6111111111111, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24653035, Length=513, Percent_Identity=22.4171539961014, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24581924, Length=309, Percent_Identity=28.8025889967638, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI161076582, Length=493, Percent_Identity=22.920892494929, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130357, Length=297, Percent_Identity=30.3030303030303, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356441, Length=497, Percent_Identity=22.7364185110664, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281365686, Length=369, Percent_Identity=22.4932249322493, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648257, Length=309, Percent_Identity=26.537216828479, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648260, Length=513, Percent_Identity=22.2222222222222, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667959, Length=278, Percent_Identity=27.3381294964029, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI62472339, Length=278, Percent_Identity=27.3381294964029, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24667955, Length=278, Percent_Identity=27.3381294964029, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 52925; Mature: 52794

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIISLSRIVAHWAAQQPSRTVLSHEGRDVSWAEFEARTNRLARAYQKLGVKQDDFVTIS
CCEEHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LPNGIEFFEACFATWKAGATPQPISARLPKLERDQIVEIGAPSLVVGVPGGEYPQTACVP
CCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCC
QGFEPDTGLSDAPLPEVTAASYKAMTSGGSTGRPKLIVSKQPAAADPDVPMLEIPQQGCM
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCE
LIPGPLYHNGPFLWAMTALFKGCTIVVTTRFDAEETLKLIEKHKADVIYTVPTMMRRIWS
EECCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHHHHC
LPEEVRASYDLSSLKALWHLAAPCPAWLKECFIEWLGPEVVWELYGGTEGQGSTTIQGTD
CCHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEECCH
WLKHKGSVGKPVETCEMKIVDEDGKTLPPREVGEVFIRPLAGAGTTYRYIGADAKAIDGG
HHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
WESLGDLGWMDEDGFLYLSDRLSDMVIVGGANIYPAEVEAAIEAYPGVRSSAVIGLPDED
HHHHHCCCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCC
MGARLHAVIDRPEGPVDDATMIAHLSERLVRYKIPKSFEYSAEAVRDDAGKVRRKALREA
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RLAAS
HHCCC
>Mature Secondary Structure 
AIISLSRIVAHWAAQQPSRTVLSHEGRDVSWAEFEARTNRLARAYQKLGVKQDDFVTIS
CEEHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LPNGIEFFEACFATWKAGATPQPISARLPKLERDQIVEIGAPSLVVGVPGGEYPQTACVP
CCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCC
QGFEPDTGLSDAPLPEVTAASYKAMTSGGSTGRPKLIVSKQPAAADPDVPMLEIPQQGCM
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCE
LIPGPLYHNGPFLWAMTALFKGCTIVVTTRFDAEETLKLIEKHKADVIYTVPTMMRRIWS
EECCCCCCCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEHHHHHHHHHC
LPEEVRASYDLSSLKALWHLAAPCPAWLKECFIEWLGPEVVWELYGGTEGQGSTTIQGTD
CCHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEECCH
WLKHKGSVGKPVETCEMKIVDEDGKTLPPREVGEVFIRPLAGAGTTYRYIGADAKAIDGG
HHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
WESLGDLGWMDEDGFLYLSDRLSDMVIVGGANIYPAEVEAAIEAYPGVRSSAVIGLPDED
HHHHHCCCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCCCC
MGARLHAVIDRPEGPVDDATMIAHLSERLVRYKIPKSFEYSAEAVRDDAGKVRRKALREA
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
RLAAS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254270; 1551828 [H]