Click here to switch to the map view.

The map label for this gene is gspE [H]

Identifier: 114799031

GI number: 114799031

Start: 2658829

End: 2660451

Strand: Direct

Name: gspE [H]

Synonym: HNE_2517

Alternate gene names: 114799031

Gene position: 2658829-2660451 (Clockwise)

Preceding gene: 114798670

Following gene: 114800215

Centisome position: 71.76

GC content: 62.42

Gene sequence:

>1623_bases
ATGGATTCCGGCTCCCTCCACATCGCTCCCGAACCGGAGGCAGAAGCGGCTGCCATCGACTGGCTGATCCGGGAAGGCGT
CCTCGCGGAAGCTGACATCGCGCATGCGGAAACGCTAAAACAAGGGTCCTCCCGGGACCTGCTCATCATCTTGAACCAGA
TTGGCGTCCTCGGCGATGACGCACTGGCCGAAGCCTATCACAGGGTCACCGGGCTCGGTATCTCCGACGGTCCATTCGTT
CCGCCCCCTGATCTTACCCAATCACTGAATCCTGAATTTCTCCGGGATACGCAGGCGCTGTTGCTCGGGCCGGATGGCCC
ACTGGCGGTCGTTAATCCGCTGGATGAGCGCCTCCGGCGCGGCATCGAGTTTGCCCTGGGCGTGCTGCCGGACCTTACCA
TCATCCGGGCCGGTGACTGGGCGAGAGCTTTTGGCCGCTTCTATCCGAGCGAACCCCTCCTGATTTCCAGCTCAGGGGGT
GAAGAGGACGCTTTCGCCGTGGAAATCGCTGACCAGGAACGCGATGCGCCGATCGTGCGGCAGGTGGCCGCCTGGCTCGG
AGAAGCGGCCGACCTCGGTGCATCCGATGTCCATTTCGATGCCCGGCGCAATTCCCTGGACGTGCGCTACCGGATCGACG
GCGTTCTGCAAGCGGCCGCGCGCGAGCCAAAAGCTGTGTCTGCGTCTGTCGTCTCCCGTATCAAGGTTATCGCCGACCTT
GATCTCGGTGAGCGCAACCGGTCCCAGGACGGACGATCCACGATCGTTGTGCGCGGACGCAGGCTGGATGTCCGGGTGTC
GATCATTCCGACAATAGACGGGGAGAGCGCCGTTGTGCGCCTGCTCGACCGGCCCGAGAAGCTTCTTTCACTCGAAGGAC
TCGGATTTTCCGGGGAGATTGCAACAGCCCTTGAAGCAGTCTGCAACCGGCGTCACGGGATGTTCGTTGTCGCCGGCCCG
ACGGGTAGTGGTAAGACCACCACGCTCTATTCCTGTCTCGAGAAGCTGAAAGGCCGCGGCCTCAAGATTCTGTCCGTTGA
AGATCCGGTCGAATATCATTTCGATCATGTCAGCCAGGTCCAGATCTCTGAAAAGGCCGGCCGGAATTTTGCCGGCGCCC
TGCGCTCATTCCTGCGTCATGATCCGGACGTGATCCTCGTGGGCGAAATCCGCGACAGTGAAACAGCGCAGGTTGCCGTG
CAGGCGTCGCTATCGGGGCATCTTGTTCTGGCCACCCTGCATGCGATCGATACGGCCCGGGTCCGCACCCGCCTCATCGA
CATGGGCGTCGAGCCGTTTAAGCTTGATGCCTGTCTCGTCGCATCCATGGCCCAGCGCCTGGTCAGGCTGCTCTGCCCGG
ACTGCCGGATGCCTGTACCGGTAACCGAAAACGAGGCCCGCCTGTTCGAGACCCATGGACTTGCCGTGCCGGAGTCCATC
TGCCGAGAGAAGGGTTGCCCCGCCTGCCGGATGGAGGGGTACAAAGGCCGCACGGCGCTGGCCGAGTTCGCCCAAAATGG
CGCTGCGCCCGATCGCGGCCAGACGCTAATGGCGCAGGGTCTGCAGCTGGTCATCCGTGGAGAGACCAGTTTGGCAGAAA
TAGCGGGTCTGAGCGAAGCATGA

Upstream 100 bases:

>100_bases
TGGTGTCCTATGGGGCGGACGGGAAGGAAGGCGGGTCAAAGCATGACAGTGATATCATCTGGCCAGACTACAAGCTGAAG
GATAATTAGGGCAGTACGCA

Downstream 100 bases:

>100_bases
CGCAGTTCCGCTACATTTCTGTCGGCCCAGACGGGACCCGGAAGTCTGGCCGGCTGGACGCGCCCGGCGAGGCCGATGCG
CGCCGACTCATTTCGGCAAA

Product: type II secretion system protein

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MDSGSLHIAPEPEAEAAAIDWLIREGVLAEADIAHAETLKQGSSRDLLIILNQIGVLGDDALAEAYHRVTGLGISDGPFV
PPPDLTQSLNPEFLRDTQALLLGPDGPLAVVNPLDERLRRGIEFALGVLPDLTIIRAGDWARAFGRFYPSEPLLISSSGG
EEDAFAVEIADQERDAPIVRQVAAWLGEAADLGASDVHFDARRNSLDVRYRIDGVLQAAAREPKAVSASVVSRIKVIADL
DLGERNRSQDGRSTIVVRGRRLDVRVSIIPTIDGESAVVRLLDRPEKLLSLEGLGFSGEIATALEAVCNRRHGMFVVAGP
TGSGKTTTLYSCLEKLKGRGLKILSVEDPVEYHFDHVSQVQISEKAGRNFAGALRSFLRHDPDVILVGEIRDSETAQVAV
QASLSGHLVLATLHAIDTARVRTRLIDMGVEPFKLDACLVASMAQRLVRLLCPDCRMPVPVTENEARLFETHGLAVPESI
CREKGCPACRMEGYKGRTALAEFAQNGAAPDRGQTLMAQGLQLVIRGETSLAEIAGLSEA

Sequences:

>Translated_540_residues
MDSGSLHIAPEPEAEAAAIDWLIREGVLAEADIAHAETLKQGSSRDLLIILNQIGVLGDDALAEAYHRVTGLGISDGPFV
PPPDLTQSLNPEFLRDTQALLLGPDGPLAVVNPLDERLRRGIEFALGVLPDLTIIRAGDWARAFGRFYPSEPLLISSSGG
EEDAFAVEIADQERDAPIVRQVAAWLGEAADLGASDVHFDARRNSLDVRYRIDGVLQAAAREPKAVSASVVSRIKVIADL
DLGERNRSQDGRSTIVVRGRRLDVRVSIIPTIDGESAVVRLLDRPEKLLSLEGLGFSGEIATALEAVCNRRHGMFVVAGP
TGSGKTTTLYSCLEKLKGRGLKILSVEDPVEYHFDHVSQVQISEKAGRNFAGALRSFLRHDPDVILVGEIRDSETAQVAV
QASLSGHLVLATLHAIDTARVRTRLIDMGVEPFKLDACLVASMAQRLVRLLCPDCRMPVPVTENEARLFETHGLAVPESI
CREKGCPACRMEGYKGRTALAEFAQNGAAPDRGQTLMAQGLQLVIRGETSLAEIAGLSEA
>Mature_540_residues
MDSGSLHIAPEPEAEAAAIDWLIREGVLAEADIAHAETLKQGSSRDLLIILNQIGVLGDDALAEAYHRVTGLGISDGPFV
PPPDLTQSLNPEFLRDTQALLLGPDGPLAVVNPLDERLRRGIEFALGVLPDLTIIRAGDWARAFGRFYPSEPLLISSSGG
EEDAFAVEIADQERDAPIVRQVAAWLGEAADLGASDVHFDARRNSLDVRYRIDGVLQAAAREPKAVSASVVSRIKVIADL
DLGERNRSQDGRSTIVVRGRRLDVRVSIIPTIDGESAVVRLLDRPEKLLSLEGLGFSGEIATALEAVCNRRHGMFVVAGP
TGSGKTTTLYSCLEKLKGRGLKILSVEDPVEYHFDHVSQVQISEKAGRNFAGALRSFLRHDPDVILVGEIRDSETAQVAV
QASLSGHLVLATLHAIDTARVRTRLIDMGVEPFKLDACLVASMAQRLVRLLCPDCRMPVPVTENEARLFETHGLAVPESI
CREKGCPACRMEGYKGRTALAEFAQNGAAPDRGQTLMAQGLQLVIRGETSLAEIAGLSEA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=391, Percent_Identity=43.7340153452685, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1786296, Length=338, Percent_Identity=38.7573964497041, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI87082188, Length=248, Percent_Identity=34.2741935483871, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 58188; Mature: 58188

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSGSLHIAPEPEAEAAAIDWLIREGVLAEADIAHAETLKQGSSRDLLIILNQIGVLGDD
CCCCCEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCH
ALAEAYHRVTGLGISDGPFVPPPDLTQSLNPEFLRDTQALLLGPDGPLAVVNPLDERLRR
HHHHHHHHHHCCCCCCCCCCCCCCCHHCCCHHHHCCCCEEEECCCCCEEEECHHHHHHHH
GIEFALGVLPDLTIIRAGDWARAFGRFYPSEPLLISSSGGEEDAFAVEIADQERDAPIVR
HHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCCCCHHHH
QVAAWLGEAADLGASDVHFDARRNSLDVRYRIDGVLQAAAREPKAVSASVVSRIKVIADL
HHHHHHCHHHCCCCCCEEECCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHEEEC
DLGERNRSQDGRSTIVVRGRRLDVRVSIIPTIDGESAVVRLLDRPEKLLSLEGLGFSGEI
CCCCCCCCCCCCEEEEEECCEEEEEEEEEEECCCHHHHHHHHHCCHHHHEECCCCCCCHH
ATALEAVCNRRHGMFVVAGPTGSGKTTTLYSCLEKLKGRGLKILSVEDPVEYHFDHVSQV
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCHHHE
QISEKAGRNFAGALRSFLRHDPDVILVGEIRDSETAQVAVQASLSGHLVLATLHAIDTAR
EEHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEHHHHHHHH
VRTRLIDMGVEPFKLDACLVASMAQRLVRLLCPDCRMPVPVTENEARLFETHGLAVPESI
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEECCCCCCCHHH
CREKGCPACRMEGYKGRTALAEFAQNGAAPDRGQTLMAQGLQLVIRGETSLAEIAGLSEA
HHHCCCCHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCEEEEECCCCHHHHCCCCCC
>Mature Secondary Structure
MDSGSLHIAPEPEAEAAAIDWLIREGVLAEADIAHAETLKQGSSRDLLIILNQIGVLGDD
CCCCCEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCH
ALAEAYHRVTGLGISDGPFVPPPDLTQSLNPEFLRDTQALLLGPDGPLAVVNPLDERLRR
HHHHHHHHHHCCCCCCCCCCCCCCCHHCCCHHHHCCCCEEEECCCCCEEEECHHHHHHHH
GIEFALGVLPDLTIIRAGDWARAFGRFYPSEPLLISSSGGEEDAFAVEIADQERDAPIVR
HHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCCCCHHHH
QVAAWLGEAADLGASDVHFDARRNSLDVRYRIDGVLQAAAREPKAVSASVVSRIKVIADL
HHHHHHCHHHCCCCCCEEECCCCCCEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHEEEC
DLGERNRSQDGRSTIVVRGRRLDVRVSIIPTIDGESAVVRLLDRPEKLLSLEGLGFSGEI
CCCCCCCCCCCCEEEEEECCEEEEEEEEEEECCCHHHHHHHHHCCHHHHEECCCCCCCHH
ATALEAVCNRRHGMFVVAGPTGSGKTTTLYSCLEKLKGRGLKILSVEDPVEYHFDHVSQV
HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCHHHE
QISEKAGRNFAGALRSFLRHDPDVILVGEIRDSETAQVAVQASLSGHLVLATLHAIDTAR
EEHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEHHHHHHHH
VRTRLIDMGVEPFKLDACLVASMAQRLVRLLCPDCRMPVPVTENEARLFETHGLAVPESI
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEECCCCCCCHHH
CREKGCPACRMEGYKGRTALAEFAQNGAAPDRGQTLMAQGLQLVIRGETSLAEIAGLSEA
HHHCCCCHHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHCCCEEEEECCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]