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The map label for this gene is pepA [H]

Identifier: 114798964

GI number: 114798964

Start: 2373332

End: 2374798

Strand: Reverse

Name: pepA [H]

Synonym: HNE_2273

Alternate gene names: 114798964

Gene position: 2374798-2373332 (Counterclockwise)

Preceding gene: 114798135

Following gene: 114798143

Centisome position: 64.1

GC content: 65.17

Gene sequence:

>1467_bases
ATGAAGATTACATTCGCAGACGCCGCCAAGGCCGACATCGCCGCCTTCATCGCCGATGAGGGGGCAGGCCTGCCCGGTAG
CGCAGAAGCGCTCGACAAGGCGTCCGGCGGCCTGATTTCCGAATCGCTGGAAGGCGGACGCTTTGATGGCAAAGTCGGCC
AGCAAGCGTCGGTTGTGCTGCCCAAGGGTTCGGACGCGCGCCGCGCTGTCGTGGTCGGCGGCGGCAAGCCCAAGAAACGC
GATGCCCGCGCCATGGAAAACATCGGCGCCCATTTCGTGAAGGCATTTGCCTCCTCGGGCTTCAAGTCTGCCTCCATCGC
CGCCCATTCGGCTGAAGAGGCTGCTCGTGTTGCGGCCGGCGCCAAACTCGCCGCCTACCGCTTCGACACCTATTTCACCA
AGCTGAAAGCCGACCAGAAGCCGAGCCTCTCGACCCTCACGGTCGTCTGCGACGATCCCAAGGCGGCAAAGGCCGCCTAT
GTGATGCTCGATGCCGCCACCGACGGCACCTATCTGGCGCGCGACCTTGTGAACATGCCGCCGAATGACCTGCACCCGGA
GAGCTTTGCCGAAAAGATCAAGGAACTCTCCGCCCTCGGCGTCGAAGTCGAAGTGCTCGGCGAGAAGCAGATGGCCAAGC
TCGGCATGGGCGCGCTCCTCGGCGTCGGCCAGGGCTCGGTCAAGGAAAGCCAGCTCGGCATCATGAAATGGAACGGCGGC
AAGGCGGGCGAAGATCCTGTCATTCTCGTCGGCAAGGGCGTCTGCTTTGATACCGGCGGCGTCTCGCTGAAGCCCGGCCC
CGGCATGGAAGAGATGCGCGGCGACATGGGCGGTGCCGCCGCCGTCACCGGCACGATGCGCGCGCTCGCCCAGGCGAAAG
CCAAGGTGAATGTCGTCGGCCTCATCGGCCTTGTGGAAAACATGCCTGATGGTAACGCCATCCGCCCCGGCGACATCCTC
CGCTCCGCTTCGGGCCAGACCATCGAAGTGCAGAACACCGACGCCGAAGGCCGCCTCGTCCTGTGTGACGTGCTCTGGTA
CGCGCAGGAGCATTTCAAGCCGAAAGCCATCGTGGACCTTGCCACGCTGACCGGCGCCATCGTCATCTCGCTCGGCCACC
ACCATGCGGGCCTCTTCACCAACTCGGACGACCTGGCAGACGAGCTGACCAAGGCCGGCCTCGCCGAAGGCGAGCGCGTC
TGGCGCCTGCCGATGGGCTCGGAATACGACTCGCTCATCAAGTCGAAGTTCGCTGACATGCGCAACATCGGCGGGCGGGC
CGCCGGCTCGATCACCGCAGCGCAGTTCCTCAAGCGCTTCATCAAGGACGGCGTCCGCTGGGCCCACCTCGACATCGCTG
GCGTTGCCTGGGTCGAAGGCGAGAAATCCCCCACCGACCCAAGCTGGGCCTCGGGCTACGGCCCGCGCCTGCTCAGCCGC
TGGATCGTGGATAACTACGAGGCCTGA

Upstream 100 bases:

>100_bases
GCGTCCAGAAACAGAACCGGAATTGGCAGCAATTCTCTAGCTAACGAGTTGCGCTTGGCGCGTCGATGCTTAGTTTCCGC
TTCAAACGGAGTTTCCACAC

Downstream 100 bases:

>100_bases
TCGGCCCCGCCTGTTTCCGAGCGCCCCCGCTTAAGGCGGGGGCCTCGATTGACCATAACAGACCCCCGCCTTCGCGGGGG
TTAGCGGAGAGAGGGTTGAG

Product: M17 family peptidase

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKITFADAAKADIAAFIADEGAGLPGSAEALDKASGGLISESLEGGRFDGKVGQQASVVLPKGSDARRAVVVGGGKPKKR
DARAMENIGAHFVKAFASSGFKSASIAAHSAEEAARVAAGAKLAAYRFDTYFTKLKADQKPSLSTLTVVCDDPKAAKAAY
VMLDAATDGTYLARDLVNMPPNDLHPESFAEKIKELSALGVEVEVLGEKQMAKLGMGALLGVGQGSVKESQLGIMKWNGG
KAGEDPVILVGKGVCFDTGGVSLKPGPGMEEMRGDMGGAAAVTGTMRALAQAKAKVNVVGLIGLVENMPDGNAIRPGDIL
RSASGQTIEVQNTDAEGRLVLCDVLWYAQEHFKPKAIVDLATLTGAIVISLGHHHAGLFTNSDDLADELTKAGLAEGERV
WRLPMGSEYDSLIKSKFADMRNIGGRAAGSITAAQFLKRFIKDGVRWAHLDIAGVAWVEGEKSPTDPSWASGYGPRLLSR
WIVDNYEA

Sequences:

>Translated_488_residues
MKITFADAAKADIAAFIADEGAGLPGSAEALDKASGGLISESLEGGRFDGKVGQQASVVLPKGSDARRAVVVGGGKPKKR
DARAMENIGAHFVKAFASSGFKSASIAAHSAEEAARVAAGAKLAAYRFDTYFTKLKADQKPSLSTLTVVCDDPKAAKAAY
VMLDAATDGTYLARDLVNMPPNDLHPESFAEKIKELSALGVEVEVLGEKQMAKLGMGALLGVGQGSVKESQLGIMKWNGG
KAGEDPVILVGKGVCFDTGGVSLKPGPGMEEMRGDMGGAAAVTGTMRALAQAKAKVNVVGLIGLVENMPDGNAIRPGDIL
RSASGQTIEVQNTDAEGRLVLCDVLWYAQEHFKPKAIVDLATLTGAIVISLGHHHAGLFTNSDDLADELTKAGLAEGERV
WRLPMGSEYDSLIKSKFADMRNIGGRAAGSITAAQFLKRFIKDGVRWAHLDIAGVAWVEGEKSPTDPSWASGYGPRLLSR
WIVDNYEA
>Mature_488_residues
MKITFADAAKADIAAFIADEGAGLPGSAEALDKASGGLISESLEGGRFDGKVGQQASVVLPKGSDARRAVVVGGGKPKKR
DARAMENIGAHFVKAFASSGFKSASIAAHSAEEAARVAAGAKLAAYRFDTYFTKLKADQKPSLSTLTVVCDDPKAAKAAY
VMLDAATDGTYLARDLVNMPPNDLHPESFAEKIKELSALGVEVEVLGEKQMAKLGMGALLGVGQGSVKESQLGIMKWNGG
KAGEDPVILVGKGVCFDTGGVSLKPGPGMEEMRGDMGGAAAVTGTMRALAQAKAKVNVVGLIGLVENMPDGNAIRPGDIL
RSASGQTIEVQNTDAEGRLVLCDVLWYAQEHFKPKAIVDLATLTGAIVISLGHHHAGLFTNSDDLADELTKAGLAEGERV
WRLPMGSEYDSLIKSKFADMRNIGGRAAGSITAAQFLKRFIKDGVRWAHLDIAGVAWVEGEKSPTDPSWASGYGPRLLSR
WIVDNYEA

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=358, Percent_Identity=39.6648044692737, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI47155554, Length=412, Percent_Identity=33.252427184466, Blast_Score=161, Evalue=2e-39,
Organism=Escherichia coli, GI1790710, Length=453, Percent_Identity=41.9426048565121, Blast_Score=326, Evalue=2e-90,
Organism=Escherichia coli, GI87082123, Length=308, Percent_Identity=36.3636363636364, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17556903, Length=346, Percent_Identity=34.971098265896, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17565172, Length=221, Percent_Identity=33.0316742081448, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355725, Length=292, Percent_Identity=41.0958904109589, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24661038, Length=294, Percent_Identity=40.4761904761905, Blast_Score=206, Evalue=4e-53,
Organism=Drosophila melanogaster, GI20129969, Length=444, Percent_Identity=28.8288288288288, Blast_Score=185, Evalue=5e-47,
Organism=Drosophila melanogaster, GI161077148, Length=352, Percent_Identity=31.25, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI20130057, Length=352, Percent_Identity=31.25, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI20129963, Length=466, Percent_Identity=30.0429184549356, Blast_Score=178, Evalue=8e-45,
Organism=Drosophila melanogaster, GI24662227, Length=343, Percent_Identity=31.4868804664723, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI21355645, Length=449, Percent_Identity=26.9487750556793, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24662223, Length=449, Percent_Identity=26.9487750556793, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI19922386, Length=452, Percent_Identity=28.7610619469027, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21357381, Length=325, Percent_Identity=33.8461538461538, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI221379063, Length=325, Percent_Identity=33.8461538461538, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI221379062, Length=325, Percent_Identity=33.8461538461538, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24646701, Length=277, Percent_Identity=29.9638989169675, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24646703, Length=277, Percent_Identity=29.9638989169675, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21358201, Length=277, Percent_Identity=29.9638989169675, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042
- InterPro:   IPR008283 [H]

Pfam domain/function: PF00883 Peptidase_M17; PF02789 Peptidase_M17_N [H]

EC number: =3.4.11.1; =3.4.11.10 [H]

Molecular weight: Translated: 51154; Mature: 51154

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITFADAAKADIAAFIADEGAGLPGSAEALDKASGGLISESLEGGRFDGKVGQQASVVL
CEEEECCCCHHHHEEEEECCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEEE
PKGSDARRAVVVGGGKPKKRDARAMENIGAHFVKAFASSGFKSASIAAHSAEEAARVAAG
ECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
AKLAAYRFDTYFTKLKADQKPSLSTLTVVCDDPKAAKAAYVMLDAATDGTYLARDLVNMP
CCCEEEEHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCC
PNDLHPESFAEKIKELSALGVEVEVLGEKQMAKLGMGALLGVGQGSVKESQLGIMKWNGG
CCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCHHEEECCCCCCCCCCEEEEEECCC
KAGEDPVILVGKGVCFDTGGVSLKPGPGMEEMRGDMGGAAAVTGTMRALAQAKAKVNVVG
CCCCCCEEEEECCEEEECCCEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEE
LIGLVENMPDGNAIRPGDILRSASGQTIEVQNTDAEGRLVLCDVLWYAQEHFKPKAIVDL
EEEHHHCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCCHHHHHH
ATLTGAIVISLGHHHAGLFTNSDDLADELTKAGLAEGERVWRLPMGSEYDSLIKSKFADM
HHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHH
RNIGGRAAGSITAAQFLKRFIKDGVRWAHLDIAGVAWVEGEKSPTDPSWASGYGPRLLSR
HHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHH
WIVDNYEA
HHHCCCCC
>Mature Secondary Structure
MKITFADAAKADIAAFIADEGAGLPGSAEALDKASGGLISESLEGGRFDGKVGQQASVVL
CEEEECCCCHHHHEEEEECCCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEEEE
PKGSDARRAVVVGGGKPKKRDARAMENIGAHFVKAFASSGFKSASIAAHSAEEAARVAAG
ECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
AKLAAYRFDTYFTKLKADQKPSLSTLTVVCDDPKAAKAAYVMLDAATDGTYLARDLVNMP
CCCEEEEHHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCC
PNDLHPESFAEKIKELSALGVEVEVLGEKQMAKLGMGALLGVGQGSVKESQLGIMKWNGG
CCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHCCHHEEECCCCCCCCCCEEEEEECCC
KAGEDPVILVGKGVCFDTGGVSLKPGPGMEEMRGDMGGAAAVTGTMRALAQAKAKVNVVG
CCCCCCEEEEECCEEEECCCEEECCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEE
LIGLVENMPDGNAIRPGDILRSASGQTIEVQNTDAEGRLVLCDVLWYAQEHFKPKAIVDL
EEEHHHCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCEEEEHHHHHHHHHCCCHHHHHH
ATLTGAIVISLGHHHAGLFTNSDDLADELTKAGLAEGERVWRLPMGSEYDSLIKSKFADM
HHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHH
RNIGGRAAGSITAAQFLKRFIKDGVRWAHLDIAGVAWVEGEKSPTDPSWASGYGPRLLSR
HHCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHH
WIVDNYEA
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]