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The map label for this gene is pleD [H]

Identifier: 114798852

GI number: 114798852

Start: 2385513

End: 2386880

Strand: Reverse

Name: pleD [H]

Synonym: HNE_2284

Alternate gene names: 114798852

Gene position: 2386880-2385513 (Counterclockwise)

Preceding gene: 114799980

Following gene: 114799591

Centisome position: 64.42

GC content: 64.18

Gene sequence:

>1368_bases
ATGACTGCCCGCATCCTCGTCGTGGACGACATCGAGGCGAACCGCCGCCTGCTTCAGGCCAAGCTGGAAGCCCAGTATCA
TACCGTCCTGATGGCGGAGAATGGTCCACAGGCGCTGGAAGTGGCGCAGCGTGAGAACCCCGAGATCATCCTGCTCGATG
TGATGATGCCCGGCATGGACGGGTATGAGGTCTGCCAACGGCTGAAGGCCGATCCAACGACCTCCTACATTCCCGTCGTC
ATGGTCACCGCCCTCAGCGAGATGGAAGACCGTGTCAAAGGCCTCGCCGCCGGCGCCGAAGACTTCCTCACCAAGCCCGT
GGACGACTTCCTGCTCAATTCCCGCATCACCGCCCTGATGCGTTACAACACGGTTGCGTCCGAACTGCGCCAGCGCGAGG
CCAGCGGCATCCGCTCCGGCGCGATGGAGGAACTCAACCGCGACGAGGTCGACCGGCCCTCGCGCATCTTCATCGTGGAC
GACGATGCCCGCTCCTCCGGGCGCCTGGCCAACACCCTGCGCGCGCAGGGCCACAAGGCCACGACCCTGATGGAAGCCGG
CGATATGGGCGAGCTTGCGACCGAGCGTGTCGACGTGCTGATCCTCTCGCTCTTTGCCCGCAGCTTTGATCCGTTGAAAC
TGATCGCGCATTTCAAGATGAACCCGGTCACCCGGTCCATTTCGGTAATCCTGATCTGCGATCCCCATGACAAGGCCAAG
GCCCTGCGGGGGCTGGAAATCGGCGCTAGTGACATGATCACCATCCCGATCGACCGGCAGGAACTGGCCGCGCGCATCCG
CACCCAGACACGCCGCACACGGTATATCGACATCCTGCGTCAGCGCGTGGACCGGGGCCTCGAGCTTTCGGTGATCGACC
AGCTGACCGGCCTGCACAACCGGCGCTACATGAACGGCCAGCTTGAACAGTTCATGCAGCGCGCTGCGCGTGGCAATCAG
CCCTTGTCGCTGATGATGACCGACATCGACCATTTCAAAGCCGTCAACGATACCCATGGCCATCAGGCAGGCGATGATGT
GCTGCGGGAAATCGGCAAGCGCCTGCGGGCGAATGTCCGGCCCACAGATGTTGTCTGCCGCACGGGCGGGGAAGAGTTTG
TGGTGCTGATGCCCAACACGCCGGGCGACCTTGCCTGCGCTGCCGCAGAGCGTATCCGCAAGGCGGTTGCGGCAGAGGAA
TTCCCCGTGCTCGGCGGGTCTCAGAACCTGCGCATCACTGTCAGCGCGGGCGTCTCCACGCTGCAGGGCGCCGGCGACAC
GACCGACGATCTGATGCACCGCGCCGACACCGCGCTCTACCAGGCCAAGACCGGCGGCCGGAACCGCGTCGAGAGCATCG
CCGCCTGA

Upstream 100 bases:

>100_bases
GCGTGCGTGAAGCCGGTTGCGAGGGCTATCTCTCCAAGCCGATCCAGATTGCCTCCTTCATCCGCGCCGTTGAAGCGCTG
ATGCCCAAGGAACCGGCGGC

Downstream 100 bases:

>100_bases
TTGACTTGGGCGCGCTAGCGGCCCACAGCGGCTGACCAGCCATACTGGAGCCTGCGCGTTCATGTCTGACCGTTTCGAAG
CGTTCCGGTATTCGTCCTAT

Product: response regulator PleD

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTARILVVDDIEANRRLLQAKLEAQYHTVLMAENGPQALEVAQRENPEIILLDVMMPGMDGYEVCQRLKADPTTSYIPVV
MVTALSEMEDRVKGLAAGAEDFLTKPVDDFLLNSRITALMRYNTVASELRQREASGIRSGAMEELNRDEVDRPSRIFIVD
DDARSSGRLANTLRAQGHKATTLMEAGDMGELATERVDVLILSLFARSFDPLKLIAHFKMNPVTRSISVILICDPHDKAK
ALRGLEIGASDMITIPIDRQELAARIRTQTRRTRYIDILRQRVDRGLELSVIDQLTGLHNRRYMNGQLEQFMQRAARGNQ
PLSLMMTDIDHFKAVNDTHGHQAGDDVLREIGKRLRANVRPTDVVCRTGGEEFVVLMPNTPGDLACAAAERIRKAVAAEE
FPVLGGSQNLRITVSAGVSTLQGAGDTTDDLMHRADTALYQAKTGGRNRVESIAA

Sequences:

>Translated_455_residues
MTARILVVDDIEANRRLLQAKLEAQYHTVLMAENGPQALEVAQRENPEIILLDVMMPGMDGYEVCQRLKADPTTSYIPVV
MVTALSEMEDRVKGLAAGAEDFLTKPVDDFLLNSRITALMRYNTVASELRQREASGIRSGAMEELNRDEVDRPSRIFIVD
DDARSSGRLANTLRAQGHKATTLMEAGDMGELATERVDVLILSLFARSFDPLKLIAHFKMNPVTRSISVILICDPHDKAK
ALRGLEIGASDMITIPIDRQELAARIRTQTRRTRYIDILRQRVDRGLELSVIDQLTGLHNRRYMNGQLEQFMQRAARGNQ
PLSLMMTDIDHFKAVNDTHGHQAGDDVLREIGKRLRANVRPTDVVCRTGGEEFVVLMPNTPGDLACAAAERIRKAVAAEE
FPVLGGSQNLRITVSAGVSTLQGAGDTTDDLMHRADTALYQAKTGGRNRVESIAA
>Mature_454_residues
TARILVVDDIEANRRLLQAKLEAQYHTVLMAENGPQALEVAQRENPEIILLDVMMPGMDGYEVCQRLKADPTTSYIPVVM
VTALSEMEDRVKGLAAGAEDFLTKPVDDFLLNSRITALMRYNTVASELRQREASGIRSGAMEELNRDEVDRPSRIFIVDD
DARSSGRLANTLRAQGHKATTLMEAGDMGELATERVDVLILSLFARSFDPLKLIAHFKMNPVTRSISVILICDPHDKAKA
LRGLEIGASDMITIPIDRQELAARIRTQTRRTRYIDILRQRVDRGLELSVIDQLTGLHNRRYMNGQLEQFMQRAARGNQP
LSLMMTDIDHFKAVNDTHGHQAGDDVLREIGKRLRANVRPTDVVCRTGGEEFVVLMPNTPGDLACAAAERIRKAVAAEEF
PVLGGSQNLRITVSAGVSTLQGAGDTTDDLMHRADTALYQAKTGGRNRVESIAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=160, Percent_Identity=37.5, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI87081881, Length=158, Percent_Identity=43.0379746835443, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=34.9397590361446, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=38.75, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI87082007, Length=159, Percent_Identity=39.622641509434, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=37.9746835443038, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787802, Length=167, Percent_Identity=37.7245508982036, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=38.4146341463415, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786599, Length=121, Percent_Identity=42.1487603305785, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788085, Length=179, Percent_Identity=33.5195530726257, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787541, Length=161, Percent_Identity=32.2981366459627, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788394, Length=135, Percent_Identity=35.5555555555556, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081974, Length=174, Percent_Identity=28.1609195402299, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081977, Length=184, Percent_Identity=29.8913043478261, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789809, Length=118, Percent_Identity=38.9830508474576, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87082012, Length=149, Percent_Identity=30.8724832214765, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788550, Length=133, Percent_Identity=33.8345864661654, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789149, Length=135, Percent_Identity=34.0740740740741, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=31.3559322033898, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=147, Percent_Identity=36.734693877551, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1786911, Length=123, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693157, Length=103, Percent_Identity=40.7766990291262, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790299, Length=104, Percent_Identity=34.6153846153846, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787375, Length=121, Percent_Identity=31.404958677686, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788191, Length=117, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790860, Length=117, Percent_Identity=31.6239316239316, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693140, Length=105, Percent_Identity=35.2380952380952, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI2367329, Length=133, Percent_Identity=39.8496240601504, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 50397; Mature: 50265

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARILVVDDIEANRRLLQAKLEAQYHTVLMAENGPQALEVAQRENPEIILLDVMMPGMD
CCEEEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCC
GYEVCQRLKADPTTSYIPVVMVTALSEMEDRVKGLAAGAEDFLTKPVDDFLLNSRITALM
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHH
RYNTVASELRQREASGIRSGAMEELNRDEVDRPSRIFIVDDDARSSGRLANTLRAQGHKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCH
TTLMEAGDMGELATERVDVLILSLFARSFDPLKLIAHFKMNPVTRSISVILICDPHDKAK
HEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEECCCHHHH
ALRGLEIGASDMITIPIDRQELAARIRTQTRRTRYIDILRQRVDRGLELSVIDQLTGLHN
HHHCCEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCC
RRYMNGQLEQFMQRAARGNQPLSLMMTDIDHFKAVNDTHGHQAGDDVLREIGKRLRANVR
CCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
PTDVVCRTGGEEFVVLMPNTPGDLACAAAERIRKAVAAEEFPVLGGSQNLRITVSAGVST
CHHEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHH
LQGAGDTTDDLMHRADTALYQAKTGGRNRVESIAA
HCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
TARILVVDDIEANRRLLQAKLEAQYHTVLMAENGPQALEVAQRENPEIILLDVMMPGMD
CEEEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCC
GYEVCQRLKADPTTSYIPVVMVTALSEMEDRVKGLAAGAEDFLTKPVDDFLLNSRITALM
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHH
RYNTVASELRQREASGIRSGAMEELNRDEVDRPSRIFIVDDDARSSGRLANTLRAQGHKA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCH
TTLMEAGDMGELATERVDVLILSLFARSFDPLKLIAHFKMNPVTRSISVILICDPHDKAK
HEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEECCCHHHH
ALRGLEIGASDMITIPIDRQELAARIRTQTRRTRYIDILRQRVDRGLELSVIDQLTGLHN
HHHCCEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCC
RRYMNGQLEQFMQRAARGNQPLSLMMTDIDHFKAVNDTHGHQAGDDVLREIGKRLRANVR
CCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
PTDVVCRTGGEEFVVLMPNTPGDLACAAAERIRKAVAAEEFPVLGGSQNLRITVSAGVST
CHHEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHH
LQGAGDTTDDLMHRADTALYQAKTGGRNRVESIAA
HCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA