Click here to switch to the map view.

The map label for this gene is norM [H]

Identifier: 114798693

GI number: 114798693

Start: 723851

End: 725266

Strand: Direct

Name: norM [H]

Synonym: HNE_0711

Alternate gene names: 114798693

Gene position: 723851-725266 (Clockwise)

Preceding gene: 114798192

Following gene: 114798808

Centisome position: 19.54

GC content: 64.9

Gene sequence:

>1416_bases
ATGACGGCGACGTCGCGCCTGCCCGCCTGGGCCAGGCCAAAGGATATAGCGGATCTGATCCGCCTGTCGGTGCCGATCGC
CGTCAGCCGCATGGCCATGATGCTCATGGGCCTGACGGATGCCATCGTTCTTGGCCAGTATGCGCCGGGCGAGCTGGCCT
ATGTGTTGTCGGCATGGCTACCCATCAGCATCTCGCTGGGCTTCGGCGTCGGCATCCTCCTGGGCATTCAGGTGCTCACC
TCTGAACTGCTCGGAACGGGCAGGGAGGCGGGCTCCGGCCGGGTGTTCCGGCGCGGTCTCTGGTGGGCCGTGGTGATGGG
CGCCGTGCTCACGGCGGTGCTCCTGCCTATTTCCGATCCGTTCTTTCACTGGGTGTTCGTCACGATTGCGCCTGCGAGCG
ATGCCCCGTCGGCTGTCACGCCAGAGATCGTGGCCAGCTCGACCGCATCGGTTACGCGCATCCTCGCGCTTGGGCTTGCC
GGGCACATGATTTCCCAGGCCTGTTCGTACTATCTGGAGGCGCTGCGCCGGCCGCTGCTCGTCACGGTCGTGATGTATGT
TGGCGTGGTCATCAACCTCTTTGCAAACCTTGCGCTCGTGGCAGGCTGGTGGGGCTTCCCCCAGATGGGCGCCGAAGGCG
TTGCCTGGGCCACTACGGGCACACGCTGGCTGATTACGATTGTCCTGCTGATCTTCGTGGCAACGCTGACGCCCGCTTTC
CGCCGCTCGCCGCCGGCGGAAAAAGGGGAGTCACGTCGTCAGCTCGCCGTTGGCGTGGGCACGGCCATTTCCAACATCGC
CGAATATGGCGGCTTCAACATCACCTTCATCATCGCCACCTGGGTCTCCATCGCGGCCAACGCCGTCTATGGCTATTCGG
TACAGGTCATCGGCGTGTGCTTCATGTTCTATCTGGGCATCGCGACAGCCACCTCCGTGCGCGTGGCCGAAGCCATCGGC
CGGGGCAGCCAGGAAGAAGTGCGCAATGCCGGGCGGCTCGGCGTTGCGGCGACCTTCGTGATGGGGATCATTCTTGGGGT
CTTGCTGGTTCTCCTGAGCGGGCCGATCTCGCGCCTGTTGGTGCGGGAAGATGCGGTCATCGGCGGCATCGCCATAGCGC
CCGCGATTGCGGCGCTCCTCTGGCTGGCGGCGGCGGTTACGGTGTTTGACGGCCTTCAGGCAACCGCCTCCTTTGCCCTG
CGCGCGCAGGGCATGGTCTGGCTGCCCAGCGCCATCCATATCAGCTCATTCTTCCTGGTGATGATCCCGGTCTGCTACTG
GCTGGCCATTACGCTCGGCCGCGGGGCTGCCGGTGTGCTCGAAGGCGCGTTTATCGCTGTGTTCGTGGCGGGGATGGCGC
AGTTCATTCTGCTGGAATGGAAAGCGGCGCGCCCCGATGTGGAGCGCGCCGCCTGA

Upstream 100 bases:

>100_bases
CATTATCGGAATATCTTGCCCTCCATTCGCCTTTGAGGCATCACGCCCGGCTGCTCAGAAATCAGGCACTGACCTGGAAA
AGACCAAAGGGAGGGCAGTC

Downstream 100 bases:

>100_bases
TCCGGATCAGTCCTCGGTGAGGACCAGAACCTTAGATGTCTTTCAGAGCCGATGCCGGTTTGAAAGCCAGCGAGGTTTTC
TCAGGAATTTTGATCGTCTC

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTATSRLPAWARPKDIADLIRLSVPIAVSRMAMMLMGLTDAIVLGQYAPGELAYVLSAWLPISISLGFGVGILLGIQVLT
SELLGTGREAGSGRVFRRGLWWAVVMGAVLTAVLLPISDPFFHWVFVTIAPASDAPSAVTPEIVASSTASVTRILALGLA
GHMISQACSYYLEALRRPLLVTVVMYVGVVINLFANLALVAGWWGFPQMGAEGVAWATTGTRWLITIVLLIFVATLTPAF
RRSPPAEKGESRRQLAVGVGTAISNIAEYGGFNITFIIATWVSIAANAVYGYSVQVIGVCFMFYLGIATATSVRVAEAIG
RGSQEEVRNAGRLGVAATFVMGIILGVLLVLLSGPISRLLVREDAVIGGIAIAPAIAALLWLAAAVTVFDGLQATASFAL
RAQGMVWLPSAIHISSFFLVMIPVCYWLAITLGRGAAGVLEGAFIAVFVAGMAQFILLEWKAARPDVERAA

Sequences:

>Translated_471_residues
MTATSRLPAWARPKDIADLIRLSVPIAVSRMAMMLMGLTDAIVLGQYAPGELAYVLSAWLPISISLGFGVGILLGIQVLT
SELLGTGREAGSGRVFRRGLWWAVVMGAVLTAVLLPISDPFFHWVFVTIAPASDAPSAVTPEIVASSTASVTRILALGLA
GHMISQACSYYLEALRRPLLVTVVMYVGVVINLFANLALVAGWWGFPQMGAEGVAWATTGTRWLITIVLLIFVATLTPAF
RRSPPAEKGESRRQLAVGVGTAISNIAEYGGFNITFIIATWVSIAANAVYGYSVQVIGVCFMFYLGIATATSVRVAEAIG
RGSQEEVRNAGRLGVAATFVMGIILGVLLVLLSGPISRLLVREDAVIGGIAIAPAIAALLWLAAAVTVFDGLQATASFAL
RAQGMVWLPSAIHISSFFLVMIPVCYWLAITLGRGAAGVLEGAFIAVFVAGMAQFILLEWKAARPDVERAA
>Mature_470_residues
TATSRLPAWARPKDIADLIRLSVPIAVSRMAMMLMGLTDAIVLGQYAPGELAYVLSAWLPISISLGFGVGILLGIQVLTS
ELLGTGREAGSGRVFRRGLWWAVVMGAVLTAVLLPISDPFFHWVFVTIAPASDAPSAVTPEIVASSTASVTRILALGLAG
HMISQACSYYLEALRRPLLVTVVMYVGVVINLFANLALVAGWWGFPQMGAEGVAWATTGTRWLITIVLLIFVATLTPAFR
RSPPAEKGESRRQLAVGVGTAISNIAEYGGFNITFIIATWVSIAANAVYGYSVQVIGVCFMFYLGIATATSVRVAEAIGR
GSQEEVRNAGRLGVAATFVMGIILGVLLVLLSGPISRLLVREDAVIGGIAIAPAIAALLWLAAAVTVFDGLQATASFALR
AQGMVWLPSAIHISSFFLVMIPVCYWLAITLGRGAAGVLEGAFIAVFVAGMAQFILLEWKAARPDVERAA

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Escherichia coli, GI48994890, Length=423, Percent_Identity=22.4586288416076, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49778; Mature: 49646

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATSRLPAWARPKDIADLIRLSVPIAVSRMAMMLMGLTDAIVLGQYAPGELAYVLSAWL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
PISISLGFGVGILLGIQVLTSELLGTGREAGSGRVFRRGLWWAVVMGAVLTAVLLPISDP
HHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
FFHWVFVTIAPASDAPSAVTPEIVASSTASVTRILALGLAGHMISQACSYYLEALRRPLL
HHEEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTVVMYVGVVINLFANLALVAGWWGFPQMGAEGVAWATTGTRWLITIVLLIFVATLTPAF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCHHH
RRSPPAEKGESRRQLAVGVGTAISNIAEYGGFNITFIIATWVSIAANAVYGYSVQVIGVC
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHH
FMFYLGIATATSVRVAEAIGRGSQEEVRNAGRLGVAATFVMGIILGVLLVLLSGPISRLL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VREDAVIGGIAIAPAIAALLWLAAAVTVFDGLQATASFALRAQGMVWLPSAIHISSFFLV
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHH
MIPVCYWLAITLGRGAAGVLEGAFIAVFVAGMAQFILLEWKAARPDVERAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure 
TATSRLPAWARPKDIADLIRLSVPIAVSRMAMMLMGLTDAIVLGQYAPGELAYVLSAWL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
PISISLGFGVGILLGIQVLTSELLGTGREAGSGRVFRRGLWWAVVMGAVLTAVLLPISDP
HHEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
FFHWVFVTIAPASDAPSAVTPEIVASSTASVTRILALGLAGHMISQACSYYLEALRRPLL
HHEEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTVVMYVGVVINLFANLALVAGWWGFPQMGAEGVAWATTGTRWLITIVLLIFVATLTPAF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCHHH
RRSPPAEKGESRRQLAVGVGTAISNIAEYGGFNITFIIATWVSIAANAVYGYSVQVIGVC
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHH
FMFYLGIATATSVRVAEAIGRGSQEEVRNAGRLGVAATFVMGIILGVLLVLLSGPISRLL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
VREDAVIGGIAIAPAIAALLWLAAAVTVFDGLQATASFALRAQGMVWLPSAIHISSFFLV
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHH
MIPVCYWLAITLGRGAAGVLEGAFIAVFVAGMAQFILLEWKAARPDVERAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]