Click here to switch to the map view.

The map label for this gene is pbpG [H]

Identifier: 114798595

GI number: 114798595

Start: 776708

End: 778801

Strand: Direct

Name: pbpG [H]

Synonym: HNE_0768

Alternate gene names: 114798595

Gene position: 776708-778801 (Clockwise)

Preceding gene: 114799183

Following gene: 114797253

Centisome position: 20.96

GC content: 65.09

Gene sequence:

>2094_bases
GTGCCTTCACCCTGCCGTAATCTTCTATCAGTTAGGCTGCGGGTTTCAGGCAGCTTCAAGAGGTCAGGCCGGGCCGTTCA
CATCATGACAGATCGTCATCCACCGCCCCGTATGCCCCGTGTGATCCGGGTGCCCGCACAGGCCCTTGCCGCGCGGCGGC
TCAGCCCGTTGCGCGTCGCGCTGATCGTCATCGGCTTCCTCGTTCTCTGCGGCGGTGGCTGGGCCCTCTGGAAATGGCAG
AGCCTGCATTCAGGCATGCCCAAACTCCCCGAAGTCGCTGATCTCTGGCAGGCCCAGCGCGAACCCGCCATCGAGTTCAT
CGACAATGAAGGCAACACGCTTGCCGTGCGCGGCCCCCGCTATGGCCGCGCCATCGCCATCGCCGATCTTCCCCGCCACG
TCCCCCAGGCCTTCATCGCGGCTGAGGATAAACGCTTCTACGAACATGACGGCGCCGATGAGGCCGCCATCGCCCGCGCC
GCCATCTCCAACGCCGCCGCCGGCGAAACCGTCTCGGGCGCCTCCACCATCACCCAACAGCTGGTGAAAAACCTCGTCCT
CAGCTCGCGCCAGACGATGCAGCGCAAGGCCCAGGAAATCACGCTCGCCCGCCAGCTCGAGAAAAAGCTCACCAAGGACG
AGATCCTCTCGCTCTATCTCAACCGTATCTATTTCGGCGCCGGCCTCTACGGCATCGACGCGGCTTCCCGCCACTATTTC
GGCAAGCCCCCGGCGGAGCTCAAAATCCACGAAGCCGCGCTGCTGGCCGCCCTCCCCAAGGCCCCGTCCAAGCTCAACCT
GCGCGAAAACCTCGAAGGCGCCAAAGCCCGCCAGCTCTACGTGCTGAAGGAAATGGAGAGCGAACGCTTCATCTCCAAAC
AGGAAGCCGCCGCCGCGCGCGAAGCGGAAATCAACATCATCGCGCCGCCGAAATATGACCCCCAGCTCGGCTATGTGCTC
GATGCCGCCGCCGAGCGTCTGACCACGATGCTGCCCCGCGTGCCGGGCGACGCGGTTGTTCAGCTCACGATCAATCCGAA
AATCCAGCAGAAAACCCAGACCCTGCTGACAGAACGCCTCGCCAGGGATGGCAAGGCGCTCGCCGCCAGCCAGGTCTCCG
CCATGATCGTGGACCGCAACGGCCGCGTCGTCGCCCTCACCGGCGGGGTCGATTACGCCGCTTCGCCCTATAACCGGGCC
ACCCAGTCCAAACGCCAGCCCGGCTCCAGCTTCAAGCCCTTCGTCTTCGCCCTTGCGCTGGAAGACGGCTACTCGCCCTA
TGACGTCTTCAATGACCGCCCGATCACCATCGGCAAGTGGCAGCCGGTCAATTACAGCGGCGAGTATCACGGCCCCATGA
CGCTGTCTGAAGCGCTCACCCGCTCCACCAACACCGTCGCGGCCGAACTCGGCAATGAAACCAATCCCGAGCGGGTCGCC
GCCCTCGCCAAACGCTTCGGCATCGAGAGCGAGCTGAAACCCTTTCCCTCCATTGCGCTGGGCAGCCAGGAAGTCTCCCT
TTGGGAACTGGTGCGGGCCTTTGGCGTGTTCCAGTCAGGCGGCTACCGGCTTGAGCCTTACCTGATCGAGCGGGTGACCG
ACTCGCGCGGCGCCGTCTATTACGAACACCCCGCTTCGGAACGCGAACGTGTGTACCCGGAAGACCTGACCGCCGAAATG
AACGCCATGATGATGCGCGTCGTCAGCGCCACCTTCGGCACCGGCGGGCGCGCCCGCATTCCGGGCTGGACCGTCGCGGG
CAAGACCGGCACCAGCCAGGAGTCGCGCGACGCCTGGTTCGTCGGCTTTACGGCGGCCTATCTCGGCGGCGTCTGGGTCG
GCAATGATGATGACACGCCAATGAAGCGCGTCACTGGCGGCACCCTCCCGGCGGAGCTCTGGTCCGACATCATGGAAATC
GCCCATGAAGGCCAGAAACCCGAAGCCCTCTTCGGCGCCAGCCGCGCGGTGATCCTTGATCCGGCGGCAGAGCAGCGCAT
CACCTTCTATCGCGGCATGGCCCAGGCCTTCGCAGCCGCCTCCGGCTCCTCCCAGACCGCCAGCCGCGGCGGCCCTGTTC
AGCAGGCGGAATAG

Upstream 100 bases:

>100_bases
CGGTCGGGCAATCGCCGGGCCGCATCGGCGCGGTCTTCCGAGCCTTACCCTGCAACAGGGCCGCATCAAGGCAAACCCGT
AGAATTCCCTATACCAGCGG

Downstream 100 bases:

>100_bases
GCCAAAGAACAGAATAGGGGCGGGCCATTTCGGGCCGGCCTTCGAAGTCAGTTCTTGTATTTGAAGATGTATTCCGTAAA
CGGAATGACTGCTCCCGCGG

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MPSPCRNLLSVRLRVSGSFKRSGRAVHIMTDRHPPPRMPRVIRVPAQALAARRLSPLRVALIVIGFLVLCGGGWALWKWQ
SLHSGMPKLPEVADLWQAQREPAIEFIDNEGNTLAVRGPRYGRAIAIADLPRHVPQAFIAAEDKRFYEHDGADEAAIARA
AISNAAAGETVSGASTITQQLVKNLVLSSRQTMQRKAQEITLARQLEKKLTKDEILSLYLNRIYFGAGLYGIDAASRHYF
GKPPAELKIHEAALLAALPKAPSKLNLRENLEGAKARQLYVLKEMESERFISKQEAAAAREAEINIIAPPKYDPQLGYVL
DAAAERLTTMLPRVPGDAVVQLTINPKIQQKTQTLLTERLARDGKALAASQVSAMIVDRNGRVVALTGGVDYAASPYNRA
TQSKRQPGSSFKPFVFALALEDGYSPYDVFNDRPITIGKWQPVNYSGEYHGPMTLSEALTRSTNTVAAELGNETNPERVA
ALAKRFGIESELKPFPSIALGSQEVSLWELVRAFGVFQSGGYRLEPYLIERVTDSRGAVYYEHPASERERVYPEDLTAEM
NAMMMRVVSATFGTGGRARIPGWTVAGKTGTSQESRDAWFVGFTAAYLGGVWVGNDDDTPMKRVTGGTLPAELWSDIMEI
AHEGQKPEALFGASRAVILDPAAEQRITFYRGMAQAFAAASGSSQTASRGGPVQQAE

Sequences:

>Translated_697_residues
MPSPCRNLLSVRLRVSGSFKRSGRAVHIMTDRHPPPRMPRVIRVPAQALAARRLSPLRVALIVIGFLVLCGGGWALWKWQ
SLHSGMPKLPEVADLWQAQREPAIEFIDNEGNTLAVRGPRYGRAIAIADLPRHVPQAFIAAEDKRFYEHDGADEAAIARA
AISNAAAGETVSGASTITQQLVKNLVLSSRQTMQRKAQEITLARQLEKKLTKDEILSLYLNRIYFGAGLYGIDAASRHYF
GKPPAELKIHEAALLAALPKAPSKLNLRENLEGAKARQLYVLKEMESERFISKQEAAAAREAEINIIAPPKYDPQLGYVL
DAAAERLTTMLPRVPGDAVVQLTINPKIQQKTQTLLTERLARDGKALAASQVSAMIVDRNGRVVALTGGVDYAASPYNRA
TQSKRQPGSSFKPFVFALALEDGYSPYDVFNDRPITIGKWQPVNYSGEYHGPMTLSEALTRSTNTVAAELGNETNPERVA
ALAKRFGIESELKPFPSIALGSQEVSLWELVRAFGVFQSGGYRLEPYLIERVTDSRGAVYYEHPASERERVYPEDLTAEM
NAMMMRVVSATFGTGGRARIPGWTVAGKTGTSQESRDAWFVGFTAAYLGGVWVGNDDDTPMKRVTGGTLPAELWSDIMEI
AHEGQKPEALFGASRAVILDPAAEQRITFYRGMAQAFAAASGSSQTASRGGPVQQAE
>Mature_696_residues
PSPCRNLLSVRLRVSGSFKRSGRAVHIMTDRHPPPRMPRVIRVPAQALAARRLSPLRVALIVIGFLVLCGGGWALWKWQS
LHSGMPKLPEVADLWQAQREPAIEFIDNEGNTLAVRGPRYGRAIAIADLPRHVPQAFIAAEDKRFYEHDGADEAAIARAA
ISNAAAGETVSGASTITQQLVKNLVLSSRQTMQRKAQEITLARQLEKKLTKDEILSLYLNRIYFGAGLYGIDAASRHYFG
KPPAELKIHEAALLAALPKAPSKLNLRENLEGAKARQLYVLKEMESERFISKQEAAAAREAEINIIAPPKYDPQLGYVLD
AAAERLTTMLPRVPGDAVVQLTINPKIQQKTQTLLTERLARDGKALAASQVSAMIVDRNGRVVALTGGVDYAASPYNRAT
QSKRQPGSSFKPFVFALALEDGYSPYDVFNDRPITIGKWQPVNYSGEYHGPMTLSEALTRSTNTVAAELGNETNPERVAA
LAKRFGIESELKPFPSIALGSQEVSLWELVRAFGVFQSGGYRLEPYLIERVTDSRGAVYYEHPASERERVYPEDLTAEMN
AMMMRVVSATFGTGGRARIPGWTVAGKTGTSQESRDAWFVGFTAAYLGGVWVGNDDDTPMKRVTGGTLPAELWSDIMEIA
HEGQKPEALFGASRAVILDPAAEQRITFYRGMAQAFAAASGSSQTASRGGPVQQAE

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1788867, Length=515, Percent_Identity=33.2038834951456, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1786343, Length=524, Percent_Identity=31.1068702290076, Blast_Score=198, Evalue=9e-52,
Organism=Escherichia coli, GI87082258, Length=251, Percent_Identity=39.0438247011952, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1789601, Length=149, Percent_Identity=40.2684563758389, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 76228; Mature: 76097

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPCRNLLSVRLRVSGSFKRSGRAVHIMTDRHPPPRMPRVIRVPAQALAARRLSPLRVA
CCCHHHHHHHEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHCCCHHHHH
LIVIGFLVLCGGGWALWKWQSLHSGMPKLPEVADLWQAQREPAIEFIDNEGNTLAVRGPR
HHHHHHHHHHCCCHHEEEHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEEECCC
YGRAIAIADLPRHVPQAFIAAEDKRFYEHDGADEAAIARAAISNAAAGETVSGASTITQQ
CCCEEEEEHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LVKNLVLSSRQTMQRKAQEITLARQLEKKLTKDEILSLYLNRIYFGAGLYGIDAASRHYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCCCCCCCC
GKPPAELKIHEAALLAALPKAPSKLNLRENLEGAKARQLYVLKEMESERFISKQEAAAAR
CCCCCCEEEHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EAEINIIAPPKYDPQLGYVLDAAAERLTTMLPRVPGDAVVQLTINPKIQQKTQTLLTERL
HCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHH
ARDGKALAASQVSAMIVDRNGRVVALTGGVDYAASPYNRATQSKRQPGSSFKPFVFALAL
HHCCHHHHHHHHEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEE
EDGYSPYDVFNDRPITIGKWQPVNYSGEYHGPMTLSEALTRSTNTVAAELGNETNPERVA
CCCCCCHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHCCCCCCHHHHH
ALAKRFGIESELKPFPSIALGSQEVSLWELVRAFGVFQSGGYRLEPYLIERVTDSRGAVY
HHHHHHCCCHHCCCCCCEECCCCCHHHHHHHHHHHHHHCCCEEECHHHEEHHCCCCCEEE
YEHPASERERVYPEDLTAEMNAMMMRVVSATFGTGGRARIPGWTVAGKTGTSQESRDAWF
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEE
VGFTAAYLGGVWVGNDDDTPMKRVTGGTLPAELWSDIMEIAHEGQKPEALFGASRAVILD
EEEHHHHHCCEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEC
PAAEQRITFYRGMAQAFAAASGSSQTASRGGPVQQAE
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PSPCRNLLSVRLRVSGSFKRSGRAVHIMTDRHPPPRMPRVIRVPAQALAARRLSPLRVA
CCHHHHHHHEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEECCHHHHHHHCCCHHHHH
LIVIGFLVLCGGGWALWKWQSLHSGMPKLPEVADLWQAQREPAIEFIDNEGNTLAVRGPR
HHHHHHHHHHCCCHHEEEHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEEECCC
YGRAIAIADLPRHVPQAFIAAEDKRFYEHDGADEAAIARAAISNAAAGETVSGASTITQQ
CCCEEEEEHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LVKNLVLSSRQTMQRKAQEITLARQLEKKLTKDEILSLYLNRIYFGAGLYGIDAASRHYF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEECCCCCCCCC
GKPPAELKIHEAALLAALPKAPSKLNLRENLEGAKARQLYVLKEMESERFISKQEAAAAR
CCCCCCEEEHHHHHHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EAEINIIAPPKYDPQLGYVLDAAAERLTTMLPRVPGDAVVQLTINPKIQQKTQTLLTERL
HCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHH
ARDGKALAASQVSAMIVDRNGRVVALTGGVDYAASPYNRATQSKRQPGSSFKPFVFALAL
HHCCHHHHHHHHEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEEE
EDGYSPYDVFNDRPITIGKWQPVNYSGEYHGPMTLSEALTRSTNTVAAELGNETNPERVA
CCCCCCHHCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHCCCCCCHHHHH
ALAKRFGIESELKPFPSIALGSQEVSLWELVRAFGVFQSGGYRLEPYLIERVTDSRGAVY
HHHHHHCCCHHCCCCCCEECCCCCHHHHHHHHHHHHHHCCCEEECHHHEEHHCCCCCEEE
YEHPASERERVYPEDLTAEMNAMMMRVVSATFGTGGRARIPGWTVAGKTGTSQESRDAWF
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCEEE
VGFTAAYLGGVWVGNDDDTPMKRVTGGTLPAELWSDIMEIAHEGQKPEALFGASRAVILD
EEEHHHHHCCEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEC
PAAEQRITFYRGMAQAFAAASGSSQTASRGGPVQQAE
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]