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The map label for this gene is flhA [H]

Identifier: 114798551

GI number: 114798551

Start: 557365

End: 558471

Strand: Reverse

Name: flhA [H]

Synonym: HNE_0560

Alternate gene names: 114798551

Gene position: 558471-557365 (Counterclockwise)

Preceding gene: 114798067

Following gene: 114797548

Centisome position: 15.07

GC content: 61.79

Gene sequence:

>1107_bases
ATGAAGACCCGCGCCGCCGTTGCCTTTGAAGCCAAAAAGCCGCTCGAAATTGTCGAGCTTGACCTTGAAGGGCCCAAGGC
CGGTGAAGTGCTGGTCGAAATCATGGCGACGGGCATCTGCCACACGGATGCCTACACGCTCGACGGGCTTGACAGCGAAG
GCATCTTCCCCAGCGTGCTGGGCCATGAAGGCGCCGGCATCGTCCGCGAGGTGGGCGCAGGCGTGACCAGCGTGAAGCCC
GGCGATCACGTTATCCCGCTCTACACGCCAGAATGCCGCCAGTGCAAAAGCTGCCTCTCAGGCAAGACCAATCTGTGTAC
GGCCATTCGCGCCACGCAGGGCAAGGGCCTGATGCCAGACGGCACCAGCCGCTTCAGCTATAAAGGCCAGACCGTCTTCC
ACTATATGGGCTGCTCCACCTTCTCGAACTTCACCGTCCTGCCCGAAATCGCCGTCGCCAAGATCCGCGAAGACGCGCCC
TTTGATAAAGCCTGCTATGTCGGCTGCGGCGTCACCACCGGCGTTGGCGCGGTCGTCAACACGGCCAAGGTTCAGGTCGG
CGAGAATGTCGTCGTCTTCGGTCTCGGCGGCATCGGCCTCAACGTCATCCAGGGCGCGCGCATGGCAGGCGCCGACATGA
TCGTCGGCGTCGACATCAATCCGGACAAGGAAGAGTGGGGCCGCAAATTCGGCATGACGCATTTCGTCAACCCGAAAAAC
GTCTCCGATGTCGTCGCCCATATCGTCGAGCTGACCGGCGGGGGCGCAGACTATTCGTTCGACTGCACCGGCAACACCAC
CGTGATGCGCCAAGCGCTGGAATGCTGCCATCGCGGCTGGGGCACCAGCGTCGTCATCGGCGTGGCCGAATCAGGCAAGG
AAATCAGCACACGTCCCTTCCAGCTGGTCACCGGGCGCAACTGGCGCGGCACAGCCTTCGGCGGCGCCAAGGGCCGCACG
GACGTCCCCAAGATCGTCGACTGGTACATGAACGGCAAAATCGAAATCGACCCGATGATCACCCACGTCATGACGCTGGA
CAAGATCAACGACGCTTTTGACCTGATGCATGAGGGCAAGTCGATCCGCAGCGTGGTTGTGTACTGA

Upstream 100 bases:

>100_bases
TGGATGAAGGGTCTGCCACCCTCGCTGAATAGACCTGCCGGGGAGTGGACCGGGACCGCGCAGCGGACTAGGTAAAGGTT
CGAATTTAGGAGCCTGCCCC

Downstream 100 bases:

>100_bases
TGGACGAATGGCAACTCCCCTGGACCGCCACCTGCCTGTGCGGACAAGTGAAAATGAAGATCACGGCCCCGCCTTTGGTC
TCGATGGCCTGCCATTGCAC

Product: S-(hydroxymethyl)glutathione dehydrogenase/alcohol dehydrogenase, class III

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIMATGICHTDAYTLDGLDSEGIFPSVLGHEGAGIVREVGAGVTSVKP
GDHVIPLYTPECRQCKSCLSGKTNLCTAIRATQGKGLMPDGTSRFSYKGQTVFHYMGCSTFSNFTVLPEIAVAKIREDAP
FDKACYVGCGVTTGVGAVVNTAKVQVGENVVVFGLGGIGLNVIQGARMAGADMIVGVDINPDKEEWGRKFGMTHFVNPKN
VSDVVAHIVELTGGGADYSFDCTGNTTVMRQALECCHRGWGTSVVIGVAESGKEISTRPFQLVTGRNWRGTAFGGAKGRT
DVPKIVDWYMNGKIEIDPMITHVMTLDKINDAFDLMHEGKSIRSVVVY

Sequences:

>Translated_368_residues
MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIMATGICHTDAYTLDGLDSEGIFPSVLGHEGAGIVREVGAGVTSVKP
GDHVIPLYTPECRQCKSCLSGKTNLCTAIRATQGKGLMPDGTSRFSYKGQTVFHYMGCSTFSNFTVLPEIAVAKIREDAP
FDKACYVGCGVTTGVGAVVNTAKVQVGENVVVFGLGGIGLNVIQGARMAGADMIVGVDINPDKEEWGRKFGMTHFVNPKN
VSDVVAHIVELTGGGADYSFDCTGNTTVMRQALECCHRGWGTSVVIGVAESGKEISTRPFQLVTGRNWRGTAFGGAKGRT
DVPKIVDWYMNGKIEIDPMITHVMTLDKINDAFDLMHEGKSIRSVVVY
>Mature_368_residues
MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIMATGICHTDAYTLDGLDSEGIFPSVLGHEGAGIVREVGAGVTSVKP
GDHVIPLYTPECRQCKSCLSGKTNLCTAIRATQGKGLMPDGTSRFSYKGQTVFHYMGCSTFSNFTVLPEIAVAKIREDAP
FDKACYVGCGVTTGVGAVVNTAKVQVGENVVVFGLGGIGLNVIQGARMAGADMIVGVDINPDKEEWGRKFGMTHFVNPKN
VSDVVAHIVELTGGGADYSFDCTGNTTVMRQALECCHRGWGTSVVIGVAESGKEISTRPFQLVTGRNWRGTAFGGAKGRT
DVPKIVDWYMNGKIEIDPMITHVMTLDKINDAFDLMHEGKSIRSVVVY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=60.7629427792915, Blast_Score=447, Evalue=1e-125,
Organism=Homo sapiens, GI71565152, Length=373, Percent_Identity=46.3806970509383, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=44.4444444444444, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI262073058, Length=370, Percent_Identity=45.6756756756757, Blast_Score=344, Evalue=6e-95,
Organism=Homo sapiens, GI71743840, Length=370, Percent_Identity=45.6756756756757, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=44.4444444444444, Blast_Score=338, Evalue=4e-93,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=43.9024390243902, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=41.304347826087, Blast_Score=324, Evalue=8e-89,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=41.5512465373961, Blast_Score=318, Evalue=5e-87,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=62.3306233062331, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI87082125, Length=381, Percent_Identity=25.1968503937008, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790045, Length=364, Percent_Identity=28.2967032967033, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787863, Length=356, Percent_Identity=23.876404494382, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788073, Length=364, Percent_Identity=26.9230769230769, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI226510992, Length=364, Percent_Identity=26.6483516483516, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788075, Length=366, Percent_Identity=26.2295081967213, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1786518, Length=372, Percent_Identity=25.8064516129032, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081918, Length=343, Percent_Identity=27.1137026239067, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786825, Length=254, Percent_Identity=27.5590551181102, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=54.6916890080429, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=54.4, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=54.2274052478134, Blast_Score=396, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17562584, Length=370, Percent_Identity=30.2702702702703, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71988145, Length=370, Percent_Identity=28.1081081081081, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17562582, Length=371, Percent_Identity=27.4932614555256, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=55.7951482479784, Blast_Score=440, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6323099, Length=386, Percent_Identity=28.2383419689119, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323729, Length=372, Percent_Identity=27.4193548387097, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322619, Length=356, Percent_Identity=29.4943820224719, Blast_Score=96, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319955, Length=356, Percent_Identity=29.4943820224719, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319621, Length=369, Percent_Identity=25.4742547425474, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=370, Percent_Identity=25.1351351351351, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=362, Percent_Identity=24.3093922651934, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319258, Length=287, Percent_Identity=26.4808362369338, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319257, Length=381, Percent_Identity=23.8845144356955, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=58.695652173913, Blast_Score=457, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39317; Mature: 39317

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIMATGICHTDAYTLDGLDSEGIFPSVL
CCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHH
GHEGAGIVREVGAGVTSVKPGDHVIPLYTPECRQCKSCLSGKTNLCTAIRATQGKGLMPD
CCCCCCHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEECCCCCCCCC
GTSRFSYKGQTVFHYMGCSTFSNFTVLPEIAVAKIREDAPFDKACYVGCGVTTGVGAVVN
CCCCEEECCCEEEEEECCCCCCCCEECHHHHHHHHHCCCCCCCEEEEECCCHHCHHHHEE
TAKVQVGENVVVFGLGGIGLNVIQGARMAGADMIVGVDINPDKEEWGRKFGMTHFVNPKN
EEEEEECCCEEEEEECCCCHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCEEECCCCC
VSDVVAHIVELTGGGADYSFDCTGNTTVMRQALECCHRGWGTSVVIGVAESGKEISTRPF
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCE
QLVTGRNWRGTAFGGAKGRTDVPKIVDWYMNGKIEIDPMITHVMTLDKINDAFDLMHEGK
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCC
SIRSVVVY
CCEEEEEC
>Mature Secondary Structure
MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIMATGICHTDAYTLDGLDSEGIFPSVL
CCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHH
GHEGAGIVREVGAGVTSVKPGDHVIPLYTPECRQCKSCLSGKTNLCTAIRATQGKGLMPD
CCCCCCHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEECCCCCCCCC
GTSRFSYKGQTVFHYMGCSTFSNFTVLPEIAVAKIREDAPFDKACYVGCGVTTGVGAVVN
CCCCEEECCCEEEEEECCCCCCCCEECHHHHHHHHHCCCCCCCEEEEECCCHHCHHHHEE
TAKVQVGENVVVFGLGGIGLNVIQGARMAGADMIVGVDINPDKEEWGRKFGMTHFVNPKN
EEEEEECCCEEEEEECCCCHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCEEECCCCC
VSDVVAHIVELTGGGADYSFDCTGNTTVMRQALECCHRGWGTSVVIGVAESGKEISTRPF
HHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCE
QLVTGRNWRGTAFGGAKGRTDVPKIVDWYMNGKIEIDPMITHVMTLDKINDAFDLMHEGK
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCC
SIRSVVVY
CCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]