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The map label for this gene is recF [H]

Identifier: 114798067

GI number: 114798067

Start: 558540

End: 559652

Strand: Reverse

Name: recF [H]

Synonym: HNE_0561

Alternate gene names: 114798067

Gene position: 559652-558540 (Counterclockwise)

Preceding gene: 114800522

Following gene: 114798551

Centisome position: 15.11

GC content: 69.63

Gene sequence:

>1113_bases
ATGACCGCCCTGACCCGCCTCAGCCTGACGGATTTCCGCAACTATGCCGGCCTGACGCTGCGCCTCGATGCGCGCCCCGT
CTGCCTTTACGGCTCCAATGGCGCGGGCAAGACAAACCTCCTGGAAGCCGTCAGCCAGTTCGGGCCGGGCCGCGGCCTGC
GCAGCGCGCAGCTCTCCGAGATGACCCGGCGCGATGCCCCCGGCGGCTGGGCGCTAGCGGCCACATTGGATGACGAACAG
AAAATCTCCATCACGCTGGACACCGCCGGAACCGCCAAGCGCACGGTGCGCATCGATGGCGCGCCCGCTTCGCCCGGAGA
CCTCGCCGAACGCATCCGCATCGTCTGGCTCACCCCGGCGATGGACGGGGTATTCCGGGGCGGGGCGTCTGACCGGCGCC
GCTTCTTCGACAGGCTGGTGATGGCCCACCTCCCCGCACATGGCAAAGCGGCGGCGCGCTATGACAAGGCCCTCGCCGAG
CGCAACGCCCTGATTGAGCGCGGCCATGTCGACCCCGCCTGGGCCGATGCGATTGAGGCGCGCCTCGCCGAAGCGGGCAC
CGAAATGGCCATCAACCGCGCCATCGTTCTGGAAGCTCTGCAGATCGCCATCGACGCGCGCCCCGAAGGCCATTTCCCGA
AAGCTGACCTCACCCTGGAAGGCGCGGCCGAAGCCGCCGCCCTCAAGGGTGAAGACTTCCGCACGATCTTTGATCTGCTG
GTCGACGCCTATCATAGCGGCCGCCGCCGAGACATCGGCGCGGGCCGCACCCTGTCAGGCCCCCACCGCACAGACCTCGC
CGTCATCCACCGGCCCACCGCCGCGCCCGCCGGCGAAGCCTCCACCGGCCAGCAGAAAGCGCTCTTGATCGGCCTCGTCC
TCGCCTCGGCCACCGCCCTCTCGGCAGGCGGCTCCGGCCCCTCGCCCCTTCTGCTGCTGGATGAAGCGGCCGCCCATCTC
GACCCCGACCGGCGCGCCGCCCTCTTCGACGAACTCACCGTGCTGGGCGGTCAGGCCTGGCTCACCGGCACAGAGGCCTT
CCTCTTCGAAGCCTTCGGCGACCGCGCCCAGCGTATCCGCGTGGATGAAGGGTCTGCCACCCTCGCTGAATAG

Upstream 100 bases:

>100_bases
CGCCATCCAGAAAGCCCGGCCAATCATTGCGGGCATGGAGCAGATCGCCGAGACACAGCCACAGGGCGGGCACGGACTTC
ATTCCGGCGACTGAGACGGC

Downstream 100 bases:

>100_bases
ACCTGCCGGGGAGTGGACCGGGACCGCGCAGCGGACTAGGTAAAGGTTCGAATTTAGGAGCCTGCCCCATGAAGACCCGC
GCCGCCGTTGCCTTTGAAGC

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTALTRLSLTDFRNYAGLTLRLDARPVCLYGSNGAGKTNLLEAVSQFGPGRGLRSAQLSEMTRRDAPGGWALAATLDDEQ
KISITLDTAGTAKRTVRIDGAPASPGDLAERIRIVWLTPAMDGVFRGGASDRRRFFDRLVMAHLPAHGKAAARYDKALAE
RNALIERGHVDPAWADAIEARLAEAGTEMAINRAIVLEALQIAIDARPEGHFPKADLTLEGAAEAAALKGEDFRTIFDLL
VDAYHSGRRRDIGAGRTLSGPHRTDLAVIHRPTAAPAGEASTGQQKALLIGLVLASATALSAGGSGPSPLLLLDEAAAHL
DPDRRAALFDELTVLGGQAWLTGTEAFLFEAFGDRAQRIRVDEGSATLAE

Sequences:

>Translated_370_residues
MTALTRLSLTDFRNYAGLTLRLDARPVCLYGSNGAGKTNLLEAVSQFGPGRGLRSAQLSEMTRRDAPGGWALAATLDDEQ
KISITLDTAGTAKRTVRIDGAPASPGDLAERIRIVWLTPAMDGVFRGGASDRRRFFDRLVMAHLPAHGKAAARYDKALAE
RNALIERGHVDPAWADAIEARLAEAGTEMAINRAIVLEALQIAIDARPEGHFPKADLTLEGAAEAAALKGEDFRTIFDLL
VDAYHSGRRRDIGAGRTLSGPHRTDLAVIHRPTAAPAGEASTGQQKALLIGLVLASATALSAGGSGPSPLLLLDEAAAHL
DPDRRAALFDELTVLGGQAWLTGTEAFLFEAFGDRAQRIRVDEGSATLAE
>Mature_369_residues
TALTRLSLTDFRNYAGLTLRLDARPVCLYGSNGAGKTNLLEAVSQFGPGRGLRSAQLSEMTRRDAPGGWALAATLDDEQK
ISITLDTAGTAKRTVRIDGAPASPGDLAERIRIVWLTPAMDGVFRGGASDRRRFFDRLVMAHLPAHGKAAARYDKALAER
NALIERGHVDPAWADAIEARLAEAGTEMAINRAIVLEALQIAIDARPEGHFPKADLTLEGAAEAAALKGEDFRTIFDLLV
DAYHSGRRRDIGAGRTLSGPHRTDLAVIHRPTAAPAGEASTGQQKALLIGLVLASATALSAGGSGPSPLLLLDEAAAHLD
PDRRAALFDELTVLGGQAWLTGTEAFLFEAFGDRAQRIRVDEGSATLAE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=380, Percent_Identity=27.6315789473684, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- InterPro:   IPR007406 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 39297; Mature: 39166

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALTRLSLTDFRNYAGLTLRLDARPVCLYGSNGAGKTNLLEAVSQFGPGRGLRSAQLSE
CCCCCCCHHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHH
MTRRDAPGGWALAATLDDEQKISITLDTAGTAKRTVRIDGAPASPGDLAERIRIVWLTPA
HHHHCCCCCEEEEEEECCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHCCEEEEEECCC
MDGVFRGGASDRRRFFDRLVMAHLPAHGKAAARYDKALAERNALIERGHVDPAWADAIEA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RLAEAGTEMAINRAIVLEALQIAIDARPEGHFPKADLTLEGAAEAAALKGEDFRTIFDLL
HHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEECCCHHHHHCCCCHHHHHHHHH
VDAYHSGRRRDIGAGRTLSGPHRTDLAVIHRPTAAPAGEASTGQQKALLIGLVLASATAL
HHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SAGGSGPSPLLLLDEAAAHLDPDRRAALFDELTVLGGQAWLTGTEAFLFEAFGDRAQRIR
CCCCCCCCCEEEEECHHHHCCCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCEEE
VDEGSATLAE
ECCCCCCCCC
>Mature Secondary Structure 
TALTRLSLTDFRNYAGLTLRLDARPVCLYGSNGAGKTNLLEAVSQFGPGRGLRSAQLSE
CCCCCCHHHHHHCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHH
MTRRDAPGGWALAATLDDEQKISITLDTAGTAKRTVRIDGAPASPGDLAERIRIVWLTPA
HHHHCCCCCEEEEEEECCCCEEEEEEECCCCCEEEEEECCCCCCHHHHHCCEEEEEECCC
MDGVFRGGASDRRRFFDRLVMAHLPAHGKAAARYDKALAERNALIERGHVDPAWADAIEA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RLAEAGTEMAINRAIVLEALQIAIDARPEGHFPKADLTLEGAAEAAALKGEDFRTIFDLL
HHHHCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEECCCHHHHHCCCCHHHHHHHHH
VDAYHSGRRRDIGAGRTLSGPHRTDLAVIHRPTAAPAGEASTGQQKALLIGLVLASATAL
HHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
SAGGSGPSPLLLLDEAAAHLDPDRRAALFDELTVLGGQAWLTGTEAFLFEAFGDRAQRIR
CCCCCCCCCEEEEECHHHHCCCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCEEE
VDEGSATLAE
ECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA