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The map label for this gene is nylA [H]

Identifier: 114798366

GI number: 114798366

Start: 1636129

End: 1637556

Strand: Direct

Name: nylA [H]

Synonym: HNE_1602

Alternate gene names: 114798366

Gene position: 1636129-1637556 (Clockwise)

Preceding gene: 114799218

Following gene: 114797884

Centisome position: 44.16

GC content: 63.24

Gene sequence:

>1428_bases
ATGCAGCTTTCAGACTATTCCGAATATGACGCTCTAGCGCTGGCTGAGCTCGTCCGTTCCCGGCAAGTGACAGCGCTCGA
ACTTGTCGATGCCGCGATCGAACGGATCGAGCGGCACAATCCGGCGTTGAATGCCGTCGTGCACAATGCCTTCGAAGAGG
CCCGCCAGACGGCAAAAGGTGCGCTCCCGGACGGACCGTTCAAGGGCGTGCCCTTTCTCATAAAGGATCTTGGTCAGAGA
GTGTCCGGCTGGCCCCGGACATCAGCGAGTGTCTACGCGGAGGTAAAGGCCGACAGGGATGACAGCGAGCTGGTCCGCCG
GTACCGCGCTGCCGGGCTCGTCCTTGCGGGCAAGACGAACACACCGGAATTCGGGATCCCCGGCGTAACCCAATCGGAGC
GGCTCGGCGCCTGCCGCAATCCGTGGAACCCGGACCATATTTCAGGCGGTTCTTCGGGCGGCGCGGCCGCGGCGGTGGCG
TCCGGCATGGTACCGGTTGCCCATGCGAGTGACGGACTTGGCTCGATCCGCATCCCGGCGGCTTGTTGTGGCCTAGTCGG
CATGAAGCCGACGCGTGACCGTAATCCCAATGGCGGTGAGGATTCCGATCGCGCCATCGGCTTTTCTGTCGATCACGTTG
TGTCGCGCACCGTGCGGGACAGCGCCGCTATGCTCGACGCGACAGGCTACCCGGAGCCTGCCAGTCCCTACGCTTATCCG
AAAAAGGAGCGGCCCTATCTTGAAGAGGTTGGCCGTGCACCCGGACGGCTGAAGATTTTCTGGTCTGCGCAAACGCCAAG
CGGCCGGCCGGTGGCGCCCGAAATGGCCGCTGCGACCGAGCGCACGGCAGAACATCTGGCCAAGCTCGGGCATGATGTTC
GCGAGCAGGCAATCACACTGGACTGGCGCACATTCTATCGCGCGCAGGCGGTTGTTTCGGGGTCAAACTTCGCAGCGGGC
ATGGCACGGATGGTGCAGGAAATTGGCCGCGAGCCAGGCAGCGACATCGGCCCGCTGGCCCGGCGCGGCTATGACCGGGG
CCGGGAAATCACCGGCCAGCAGGCGATGTGGGGCTGGCAACAGCTTCGGCTGATGGGCCGCATGGTTCTTGCCGTGTTCG
AGGAATGCGATGTCTATCTCTCGCCCGTGCTGGGTACCGAGCCGCCGAAGGTTGACTGGCTCGATCCGCTGAGCCTTGAC
ATCAAGGAGTATGACAAACGGTCTGCGGCGACTTTTCCGTTCACCCCGGCCTTCAACATCACCGGTCAGCCGTCCCTGTC
CCTGCCGCTCTGGCAATCTGACAACAATCTTCCGCTCGCCATGATGTTCACGGGCCGGTACGGCGACGAAGGCACCTTAT
ACCGGCTCGCCGGGCAGCTTGAGAAAGAACTTCCGTGGAAGGACCGTCGGCCACCCATCTGGAACTGA

Upstream 100 bases:

>100_bases
CGAGGTAGGTTGCGGTCGTCTTTGACAGCTCGCTTTTTCCAGGCACACTGGACAATCTCATAATTCGGTAGATCAATTGG
CAAAAATCAAGGGGTCGAGA

Downstream 100 bases:

>100_bases
CCTGGCAGATCACCCGACGAGCGGTTGAGCAGAGTTTGTCTGACGTCCTGCGGTTCAGATCCTGAGCAGCACGCGGCCCT
GTTTCCCGCGCGTTGCAAAG

Product: putative 6-aminohexanoate-cyclic-dimer hydrolase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MQLSDYSEYDALALAELVRSRQVTALELVDAAIERIERHNPALNAVVHNAFEEARQTAKGALPDGPFKGVPFLIKDLGQR
VSGWPRTSASVYAEVKADRDDSELVRRYRAAGLVLAGKTNTPEFGIPGVTQSERLGACRNPWNPDHISGGSSGGAAAAVA
SGMVPVAHASDGLGSIRIPAACCGLVGMKPTRDRNPNGGEDSDRAIGFSVDHVVSRTVRDSAAMLDATGYPEPASPYAYP
KKERPYLEEVGRAPGRLKIFWSAQTPSGRPVAPEMAAATERTAEHLAKLGHDVREQAITLDWRTFYRAQAVVSGSNFAAG
MARMVQEIGREPGSDIGPLARRGYDRGREITGQQAMWGWQQLRLMGRMVLAVFEECDVYLSPVLGTEPPKVDWLDPLSLD
IKEYDKRSAATFPFTPAFNITGQPSLSLPLWQSDNNLPLAMMFTGRYGDEGTLYRLAGQLEKELPWKDRRPPIWN

Sequences:

>Translated_475_residues
MQLSDYSEYDALALAELVRSRQVTALELVDAAIERIERHNPALNAVVHNAFEEARQTAKGALPDGPFKGVPFLIKDLGQR
VSGWPRTSASVYAEVKADRDDSELVRRYRAAGLVLAGKTNTPEFGIPGVTQSERLGACRNPWNPDHISGGSSGGAAAAVA
SGMVPVAHASDGLGSIRIPAACCGLVGMKPTRDRNPNGGEDSDRAIGFSVDHVVSRTVRDSAAMLDATGYPEPASPYAYP
KKERPYLEEVGRAPGRLKIFWSAQTPSGRPVAPEMAAATERTAEHLAKLGHDVREQAITLDWRTFYRAQAVVSGSNFAAG
MARMVQEIGREPGSDIGPLARRGYDRGREITGQQAMWGWQQLRLMGRMVLAVFEECDVYLSPVLGTEPPKVDWLDPLSLD
IKEYDKRSAATFPFTPAFNITGQPSLSLPLWQSDNNLPLAMMFTGRYGDEGTLYRLAGQLEKELPWKDRRPPIWN
>Mature_475_residues
MQLSDYSEYDALALAELVRSRQVTALELVDAAIERIERHNPALNAVVHNAFEEARQTAKGALPDGPFKGVPFLIKDLGQR
VSGWPRTSASVYAEVKADRDDSELVRRYRAAGLVLAGKTNTPEFGIPGVTQSERLGACRNPWNPDHISGGSSGGAAAAVA
SGMVPVAHASDGLGSIRIPAACCGLVGMKPTRDRNPNGGEDSDRAIGFSVDHVVSRTVRDSAAMLDATGYPEPASPYAYP
KKERPYLEEVGRAPGRLKIFWSAQTPSGRPVAPEMAAATERTAEHLAKLGHDVREQAITLDWRTFYRAQAVVSGSNFAAG
MARMVQEIGREPGSDIGPLARRGYDRGREITGQQAMWGWQQLRLMGRMVLAVFEECDVYLSPVLGTEPPKVDWLDPLSLD
IKEYDKRSAATFPFTPAFNITGQPSLSLPLWQSDNNLPLAMMFTGRYGDEGTLYRLAGQLEKELPWKDRRPPIWN

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=238, Percent_Identity=31.5126050420168, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=26.8774703557312, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=314, Percent_Identity=28.9808917197452, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17537465, Length=192, Percent_Identity=32.8125, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=185, Percent_Identity=28.1081081081081, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=171, Percent_Identity=33.9181286549708, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17556276, Length=313, Percent_Identity=27.4760383386581, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=313, Percent_Identity=27.4760383386581, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=127, Percent_Identity=39.3700787401575, Blast_Score=63, Evalue=1e-10,
Organism=Drosophila melanogaster, GI45550774, Length=320, Percent_Identity=27.8125, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648435, Length=320, Percent_Identity=27.8125, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648437, Length=320, Percent_Identity=27.8125, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24644968, Length=483, Percent_Identity=25.0517598343685, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21356731, Length=483, Percent_Identity=24.4306418219462, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI161078093, Length=514, Percent_Identity=24.5136186770428, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24652985, Length=350, Percent_Identity=24.8571428571429, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922090, Length=350, Percent_Identity=24.8571428571429, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652981, Length=350, Percent_Identity=24.8571428571429, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652983, Length=350, Percent_Identity=24.8571428571429, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648113, Length=464, Percent_Identity=26.7241379310345, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648441, Length=244, Percent_Identity=26.2295081967213, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648439, Length=244, Percent_Identity=26.2295081967213, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 51900; Mature: 51900

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLSDYSEYDALALAELVRSRQVTALELVDAAIERIERHNPALNAVVHNAFEEARQTAKG
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
ALPDGPFKGVPFLIKDLGQRVSGWPRTSASVYAEVKADRDDSELVRRYRAAGLVLAGKTN
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCEEEECCCC
TPEFGIPGVTQSERLGACRNPWNPDHISGGSSGGAAAAVASGMVPVAHASDGLGSIRIPA
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEHHH
ACCGLVGMKPTRDRNPNGGEDSDRAIGFSVDHVVSRTVRDSAAMLDATGYPEPASPYAYP
HHHHHCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KKERPYLEEVGRAPGRLKIFWSAQTPSGRPVAPEMAAATERTAEHLAKLGHDVREQAITL
CCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
DWRTFYRAQAVVSGSNFAAGMARMVQEIGREPGSDIGPLARRGYDRGREITGQQAMWGWQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHH
QLRLMGRMVLAVFEECDVYLSPVLGTEPPKVDWLDPLSLDIKEYDKRSAATFPFTPAFNI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
TGQPSLSLPLWQSDNNLPLAMMFTGRYGDEGTLYRLAGQLEKELPWKDRRPPIWN
CCCCCCCCCEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQLSDYSEYDALALAELVRSRQVTALELVDAAIERIERHNPALNAVVHNAFEEARQTAKG
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
ALPDGPFKGVPFLIKDLGQRVSGWPRTSASVYAEVKADRDDSELVRRYRAAGLVLAGKTN
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCEEEECCCC
TPEFGIPGVTQSERLGACRNPWNPDHISGGSSGGAAAAVASGMVPVAHASDGLGSIRIPA
CCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEHHH
ACCGLVGMKPTRDRNPNGGEDSDRAIGFSVDHVVSRTVRDSAAMLDATGYPEPASPYAYP
HHHHHCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
KKERPYLEEVGRAPGRLKIFWSAQTPSGRPVAPEMAAATERTAEHLAKLGHDVREQAITL
CCCCCHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
DWRTFYRAQAVVSGSNFAAGMARMVQEIGREPGSDIGPLARRGYDRGREITGQQAMWGWQ
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHH
QLRLMGRMVLAVFEECDVYLSPVLGTEPPKVDWLDPLSLDIKEYDKRSAATFPFTPAFNI
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
TGQPSLSLPLWQSDNNLPLAMMFTGRYGDEGTLYRLAGQLEKELPWKDRRPPIWN
CCCCCCCCCEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]