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The map label for this gene is cobS [H]

Identifier: 114798325

GI number: 114798325

Start: 1546083

End: 1546841

Strand: Direct

Name: cobS [H]

Synonym: HNE_1511

Alternate gene names: 114798325

Gene position: 1546083-1546841 (Clockwise)

Preceding gene: 114799398

Following gene: 114797780

Centisome position: 41.73

GC content: 63.24

Gene sequence:

>759_bases
ATGAAGGACGAGTTTGCCAAACTCTGCCTGGCCATTCAGTTCCTGACGCGCATTCGTCTGCCGTTCGAGCCCGGCTATAC
GCCGGCACGGATGGCAATGACCCCCGCTTACTATCCACTCGTCGGAGCAATGATAGGCGGGATGTGTGCCGGGGTCTTCT
GGCTTGCGGCGCTTGGTTTGCCGGGGCCTGTCAGCGTTGTCCTGGCCCTCGGCGCAGGCTTGCTCCTGACCGGGGCCTTC
CATGAGGATGGCCTGGCAGACACATTTGATGGAATTGGCGGCGGGCTGACGCGCGAGCAGGCGCTGACCATTATGAAGGA
CAGCCGGATCGGCACCTATGGCACAGCGGCGCTGGCCGGCATCCTGCTTCTCAAAGCCGCTGTTCTGTCCTTCCTGCCGG
GCTCGCTGATTGTAATGGCGTTCGTGGCCGGACATGCGCTCTCCCGGCTCTCGGCCGTAATGGTCATCGTGACGAGCCGC
TATGTCCGCGACGAGGGGACAGGGAAGCCTGTCGCTCAGGGCCTTTCAGGCGGGGGACTGGCAATCGCCTGTCTTACGGG
CGCGCTCATCCTCGGGCTTTGGCCGCTCGTGCAACCATTCCCCGCAGTGTTCTACGGGCTGGGCGGAGTGATTGCCGGCC
ATGCACTTATGCGGCTGTTCTTTGAACGCAAGCTTGGCGGCTATACCGGCGACACCCTGGGCGCTGTCCAGCAGGTCAGC
GAGGCGGGCTTTTATCTGGGCCTGCTCCTGTGGCTCTGA

Upstream 100 bases:

>100_bases
AGGGCACGGGCGCGCTTCTGGCCTGGCCTCTGGTGCAGGCCGCCGCTGCGATGCTGCGCGACATGGCAAGTTTTGAAAGC
GCTGGTGTGAGCGGTCCGGC

Downstream 100 bases:

>100_bases
TCCTGCTGCGTCATACCCGTCCCGATGTGCTCACCGGTACCTGCTATGGAGCGCGGGACGTTGGATTGGCGGCGACCTTT
GCCGGGGAAGCCGAAGCACT

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKDEFAKLCLAIQFLTRIRLPFEPGYTPARMAMTPAYYPLVGAMIGGMCAGVFWLAALGLPGPVSVVLALGAGLLLTGAF
HEDGLADTFDGIGGGLTREQALTIMKDSRIGTYGTAALAGILLLKAAVLSFLPGSLIVMAFVAGHALSRLSAVMVIVTSR
YVRDEGTGKPVAQGLSGGGLAIACLTGALILGLWPLVQPFPAVFYGLGGVIAGHALMRLFFERKLGGYTGDTLGAVQQVS
EAGFYLGLLLWL

Sequences:

>Translated_252_residues
MKDEFAKLCLAIQFLTRIRLPFEPGYTPARMAMTPAYYPLVGAMIGGMCAGVFWLAALGLPGPVSVVLALGAGLLLTGAF
HEDGLADTFDGIGGGLTREQALTIMKDSRIGTYGTAALAGILLLKAAVLSFLPGSLIVMAFVAGHALSRLSAVMVIVTSR
YVRDEGTGKPVAQGLSGGGLAIACLTGALILGLWPLVQPFPAVFYGLGGVIAGHALMRLFFERKLGGYTGDTLGAVQQVS
EAGFYLGLLLWL
>Mature_252_residues
MKDEFAKLCLAIQFLTRIRLPFEPGYTPARMAMTPAYYPLVGAMIGGMCAGVFWLAALGLPGPVSVVLALGAGLLLTGAF
HEDGLADTFDGIGGGLTREQALTIMKDSRIGTYGTAALAGILLLKAAVLSFLPGSLIVMAFVAGHALSRLSAVMVIVTSR
YVRDEGTGKPVAQGLSGGGLAIACLTGALILGLWPLVQPFPAVFYGLGGVIAGHALMRLFFERKLGGYTGDTLGAVQQVS
EAGFYLGLLLWL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=249, Percent_Identity=34.9397590361446, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26209; Mature: 26209

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEFAKLCLAIQFLTRIRLPFEPGYTPARMAMTPAYYPLVGAMIGGMCAGVFWLAALGL
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
PGPVSVVLALGAGLLLTGAFHEDGLADTFDGIGGGLTREQALTIMKDSRIGTYGTAALAG
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
ILLLKAAVLSFLPGSLIVMAFVAGHALSRLSAVMVIVTSRYVRDEGTGKPVAQGLSGGGL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCH
AIACLTGALILGLWPLVQPFPAVFYGLGGVIAGHALMRLFFERKLGGYTGDTLGAVQQVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EAGFYLGLLLWL
HHHHHHHHHHHC
>Mature Secondary Structure
MKDEFAKLCLAIQFLTRIRLPFEPGYTPARMAMTPAYYPLVGAMIGGMCAGVFWLAALGL
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
PGPVSVVLALGAGLLLTGAFHEDGLADTFDGIGGGLTREQALTIMKDSRIGTYGTAALAG
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHH
ILLLKAAVLSFLPGSLIVMAFVAGHALSRLSAVMVIVTSRYVRDEGTGKPVAQGLSGGGL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCH
AIACLTGALILGLWPLVQPFPAVFYGLGGVIAGHALMRLFFERKLGGYTGDTLGAVQQVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EAGFYLGLLLWL
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA