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The map label for this gene is gspO [H]

Identifier: 114798317

GI number: 114798317

Start: 2813620

End: 2814276

Strand: Direct

Name: gspO [H]

Synonym: HNE_2653

Alternate gene names: 114798317

Gene position: 2813620-2814276 (Clockwise)

Preceding gene: 114797300

Following gene: 114798339

Centisome position: 75.94

GC content: 64.08

Gene sequence:

>657_bases
ATGACGGGCAGTGATCTCGGCCTCGTGCTTCTGGGTTTCCTGATTGGCGCCGGCATTGAAGGGGCTGCCAGCCGGTTTGC
CGGGGAGAGACGCACACTTCGAGACGTCTGGTTGAGCGCGCGCACGGTTTTCACAGCGATTGCCGGGGCGCTGGTGGCCC
TCGCCGCCGGGCTCTACGGCTCCAACAACCTCCCGATCTTGAGTTTTACACTGCTGTTCGGGTGGGCGCTTCTTGCTTTG
GCCGCCATTGACCTGCGCACTTATCTGCTGCCCGACAGGCTGAACCTTGCCGTGTTTCTGTTGGGCGCGGGAATGATTGC
GTTATACCGGCAGGAGGTGTGGCCCTGGCATCTGGCCGGCGCGATCACCGGATATGGTCTGCTCTGGCTGGTGGGCGTGG
CGTACCGCCGATTGCGCGGGGTCGACGGGCTCGGCCGCGGTGATGCCAAGCTGCTCGGCGCCATTGGAATGTGGGTCGGC
CTGCAAGGCATTCCGCCCGTCCTGTTGATTGCATCGCTGAGCGGCATACTGGCGGTTCTTGCCGGCTCCGCCATCCGGCG
CGAGTCTGTCTCCGGACAATCCATGATCGCCTTCGGCCCCTGGATCGCGCTGGCCGGATACATCGTCTGGCTGATGCCGG
CGCTTCTGCCGTATTGA

Upstream 100 bases:

>100_bases
ATGCAGCCAAAACGGCGAACTGAAGCGGGCGCTGCCTCGGGCACGGCAGGATTTGCCGCGCCTGCGCGCCGTAACGGCGC
CCAATCCCGTGTGACACGCC

Downstream 100 bases:

>100_bases
CCCGCGCAGCGCAGGCTGCGGTATAGCCCCACTCCGCTGAACCTCTTCGCTGAAACTGGCGCCAATCCTGCCCGCTCAAC
AGGTGTCGGAAGGAAACGCA

Product: prepilin peptidase

Products: NA

Alternate protein names: Pectic enzymes secretion protein outO; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTGSDLGLVLLGFLIGAGIEGAASRFAGERRTLRDVWLSARTVFTAIAGALVALAAGLYGSNNLPILSFTLLFGWALLAL
AAIDLRTYLLPDRLNLAVFLLGAGMIALYRQEVWPWHLAGAITGYGLLWLVGVAYRRLRGVDGLGRGDAKLLGAIGMWVG
LQGIPPVLLIASLSGILAVLAGSAIRRESVSGQSMIAFGPWIALAGYIVWLMPALLPY

Sequences:

>Translated_218_residues
MTGSDLGLVLLGFLIGAGIEGAASRFAGERRTLRDVWLSARTVFTAIAGALVALAAGLYGSNNLPILSFTLLFGWALLAL
AAIDLRTYLLPDRLNLAVFLLGAGMIALYRQEVWPWHLAGAITGYGLLWLVGVAYRRLRGVDGLGRGDAKLLGAIGMWVG
LQGIPPVLLIASLSGILAVLAGSAIRRESVSGQSMIAFGPWIALAGYIVWLMPALLPY
>Mature_217_residues
TGSDLGLVLLGFLIGAGIEGAASRFAGERRTLRDVWLSARTVFTAIAGALVALAAGLYGSNNLPILSFTLLFGWALLALA
AIDLRTYLLPDRLNLAVFLLGAGMIALYRQEVWPWHLAGAITGYGLLWLVGVAYRRLRGVDGLGRGDAKLLGAIGMWVGL
QGIPPVLLIASLSGILAVLAGSAIRRESVSGQSMIAFGPWIALAGYIVWLMPALLPY

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI1789732, Length=146, Percent_Identity=40.4109589041096, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 23031; Mature: 22900

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGSDLGLVLLGFLIGAGIEGAASRFAGERRTLRDVWLSARTVFTAIAGALVALAAGLYG
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SNNLPILSFTLLFGWALLALAAIDLRTYLLPDRLNLAVFLLGAGMIALYRQEVWPWHLAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AITGYGLLWLVGVAYRRLRGVDGLGRGDAKLLGAIGMWVGLQGIPPVLLIASLSGILAVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AGSAIRRESVSGQSMIAFGPWIALAGYIVWLMPALLPY
HHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TGSDLGLVLLGFLIGAGIEGAASRFAGERRTLRDVWLSARTVFTAIAGALVALAAGLYG
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SNNLPILSFTLLFGWALLALAAIDLRTYLLPDRLNLAVFLLGAGMIALYRQEVWPWHLAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
AITGYGLLWLVGVAYRRLRGVDGLGRGDAKLLGAIGMWVGLQGIPPVLLIASLSGILAVL
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AGSAIRRESVSGQSMIAFGPWIALAGYIVWLMPALLPY
HHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]