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The map label for this gene is selB [H]

Identifier: 114798311

GI number: 114798311

Start: 2615805

End: 2617676

Strand: Reverse

Name: selB [H]

Synonym: HNE_2488

Alternate gene names: 114798311

Gene position: 2617676-2615805 (Counterclockwise)

Preceding gene: 114797888

Following gene: 114800123

Centisome position: 70.65

GC content: 65.17

Gene sequence:

>1872_bases
GTGAAGTCTTGCCCTGTCATCGTGATCGGGCATGTCGACCATGGCAAGACTTCGCTGGTGGGCGCGCTGACCGGGATAGA
AACCGACAGGCTTCCTGAAGAAAAGGCGAGGGGCCTCTCCATTACCTCCGGATTTGCCTATTTGAAGGGCGGCGATGCCA
ACATTGATCTGGTCGATGCACCCGGCCACCAGAACTTCATTCGTGCGATGGTCGGCGGCGCGGCGGGCGCCCGCAGCGCG
GCGCTGGTGGTCTCCGCCGCCGAAGGGGTCGAGGCGCAGACACTCGAACACATCGCGGTCATAGAAACGCTCGGTATCCA
TGCCGGGATCGTCGTATTGAGCAAGGCAGACCTAGTGGCGCCTTCTGAGCGGCCCGGCCGGAAAGTGGCCCTGCAGGCGG
CGTTGGCGGGAACCGCATTCCGCGACGCGCCGCTGATATTCTGCTCGGCTGTGACCGGCGAAGGCCTTGAAGATATTGCC
GCAGCGCTGCGGGGTCTTGCCGCGCTTCCGGCAGGCGCCGGACCCGCTGGCGCCTTCCTCGCCATAGACCGGGTGTTTGT
CGCCGAGGGTCACGGCACGGTGGTGACCGGCACACTGCTCGGCAGGTCGCTGAAGCCCGCGGACGAACTCTTGCTTGCGC
CCTCAGGCGAGATAGTGACGATCCGGCGGATCGAGGTGCGCGGTGAGACTGTATCAATGGCGCAGCCGGGTGAGCGTACC
GCGCTGAACCTGCGGGGCGTGGCGGCTTCCAAAATCAAACCGGGGGATGTCGTTCATGCGGCGGACGCTGGCGCAGCATC
GCTCAACCTTGACGCTGCGATTTCTGTTTCTGCCAGAGCAGGCCGTCCTGTTCGTCACATGGAGGAAATCCGTGTGCTGT
ATGGAACGGGGAACGGGGTGGCGACGCTCCGTCTGCTTGGCCGCAAAGAGGTCAGTCCTGGCGAGACCGGATTTGCCCAA
CTGCGGTTTACGGCGCCCGCCGCCGCTTTCGCGGGTCAGCGCGCTGTCCTGCGCAGCCTGTCTCCGAGCCAGACACTGGG
CGGCGCAATTATCCTTGATCCGGCGGCAACCCCTCTGACGGCCAGCAAAGCCTTACGGCTTGCAACCCTTGAGGCGGCTT
ATCGCGGTGATACGCTCGCTCTGGCCGCTGCCCTGGCTGCCGAGCAGGGCGGCGTCGCGCGCCTCGGTGATGTCTGCCGG
CTGGCACGGGCGCCGGAAGCCTCGGTGCGGGCAAGTCTCGGTGAGGCCTTCACGCAAATCGCGGACGGGTTTGTCTCGCC
GGCGGCTGTCGTGGCTGAAGCGCGGGACGCCTACCTGGCACGCCTTTGCGCCTATCACGCCGAGCACAGGTTGAGACCCC
AGGCGCCGCGTTCGGAGATATTGGCCGGGCACGTGTCCCTTCTGCTCAACCTCCACGTCGAGGAAAGCCTCGCCGCTGCG
GGCGTGATCCGGCTTTCCGGCGGCGGTGTGGCGCTTGCCATCCATGACGCGGCGGCAAACCTTACCCCGGACCAGACGGC
GCGGATGCACGGGCTTGCCCGCCAGCTCCAGGCAGACGGACTGACGCCGTCAGGGACAGCGGCGCTGATCAGGAGCCCCG
AAGATGCGGAGTTGCTCGAGCTGCTGGTTGAGAGGGGCGAGGTAATCCGGCTGAGCAATGTCTCGCTGAAGCAGACGATC
TGGTTGCACGCAGAAGCGGTCTCGTCCGCAATACAGATGCTGAGAACGGGCTTTCCGGGCGAAACGGCTTTTACGACCGG
AGAAGCGCGCGAAGCCCTGAAGACCAGCCGCAAGCATATTGTCCCGCTGCTGGAGTATCTCGACGCGGCCGGCATTACCC
AGCGGCAGGGTGATACACGCTGCATGGTATGA

Upstream 100 bases:

>100_bases
TCGGCATGATCCGGGAAGACCGGTTCGTTATGGATGTTCGTACGCTGATCGATGGAGATCTTCCCGGGATTGTGGAAGCC
TTTCGCCAGGTGGCCCTCCG

Downstream 100 bases:

>100_bases
TGTTTGACGCAGCCCCCGGCGCCGCTAGTGTTGCGCTTCTGGAGGTGACTGGAGTCTGGTGGCTTCCCTGGTCTTCAAAA
CTATGGACACGCCGGCAAGG

Product: selenocysteine-specific translation elongation factor

Products: NA

Alternate protein names: SelB translation factor [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MKSCPVIVIGHVDHGKTSLVGALTGIETDRLPEEKARGLSITSGFAYLKGGDANIDLVDAPGHQNFIRAMVGGAAGARSA
ALVVSAAEGVEAQTLEHIAVIETLGIHAGIVVLSKADLVAPSERPGRKVALQAALAGTAFRDAPLIFCSAVTGEGLEDIA
AALRGLAALPAGAGPAGAFLAIDRVFVAEGHGTVVTGTLLGRSLKPADELLLAPSGEIVTIRRIEVRGETVSMAQPGERT
ALNLRGVAASKIKPGDVVHAADAGAASLNLDAAISVSARAGRPVRHMEEIRVLYGTGNGVATLRLLGRKEVSPGETGFAQ
LRFTAPAAAFAGQRAVLRSLSPSQTLGGAIILDPAATPLTASKALRLATLEAAYRGDTLALAAALAAEQGGVARLGDVCR
LARAPEASVRASLGEAFTQIADGFVSPAAVVAEARDAYLARLCAYHAEHRLRPQAPRSEILAGHVSLLLNLHVEESLAAA
GVIRLSGGGVALAIHDAAANLTPDQTARMHGLARQLQADGLTPSGTAALIRSPEDAELLELLVERGEVIRLSNVSLKQTI
WLHAEAVSSAIQMLRTGFPGETAFTTGEAREALKTSRKHIVPLLEYLDAAGITQRQGDTRCMV

Sequences:

>Translated_623_residues
MKSCPVIVIGHVDHGKTSLVGALTGIETDRLPEEKARGLSITSGFAYLKGGDANIDLVDAPGHQNFIRAMVGGAAGARSA
ALVVSAAEGVEAQTLEHIAVIETLGIHAGIVVLSKADLVAPSERPGRKVALQAALAGTAFRDAPLIFCSAVTGEGLEDIA
AALRGLAALPAGAGPAGAFLAIDRVFVAEGHGTVVTGTLLGRSLKPADELLLAPSGEIVTIRRIEVRGETVSMAQPGERT
ALNLRGVAASKIKPGDVVHAADAGAASLNLDAAISVSARAGRPVRHMEEIRVLYGTGNGVATLRLLGRKEVSPGETGFAQ
LRFTAPAAAFAGQRAVLRSLSPSQTLGGAIILDPAATPLTASKALRLATLEAAYRGDTLALAAALAAEQGGVARLGDVCR
LARAPEASVRASLGEAFTQIADGFVSPAAVVAEARDAYLARLCAYHAEHRLRPQAPRSEILAGHVSLLLNLHVEESLAAA
GVIRLSGGGVALAIHDAAANLTPDQTARMHGLARQLQADGLTPSGTAALIRSPEDAELLELLVERGEVIRLSNVSLKQTI
WLHAEAVSSAIQMLRTGFPGETAFTTGEAREALKTSRKHIVPLLEYLDAAGITQRQGDTRCMV
>Mature_623_residues
MKSCPVIVIGHVDHGKTSLVGALTGIETDRLPEEKARGLSITSGFAYLKGGDANIDLVDAPGHQNFIRAMVGGAAGARSA
ALVVSAAEGVEAQTLEHIAVIETLGIHAGIVVLSKADLVAPSERPGRKVALQAALAGTAFRDAPLIFCSAVTGEGLEDIA
AALRGLAALPAGAGPAGAFLAIDRVFVAEGHGTVVTGTLLGRSLKPADELLLAPSGEIVTIRRIEVRGETVSMAQPGERT
ALNLRGVAASKIKPGDVVHAADAGAASLNLDAAISVSARAGRPVRHMEEIRVLYGTGNGVATLRLLGRKEVSPGETGFAQ
LRFTAPAAAFAGQRAVLRSLSPSQTLGGAIILDPAATPLTASKALRLATLEAAYRGDTLALAAALAAEQGGVARLGDVCR
LARAPEASVRASLGEAFTQIADGFVSPAAVVAEARDAYLARLCAYHAEHRLRPQAPRSEILAGHVSLLLNLHVEESLAAA
GVIRLSGGGVALAIHDAAANLTPDQTARMHGLARQLQADGLTPSGTAALIRSPEDAELLELLVERGEVIRLSNVSLKQTI
WLHAEAVSSAIQMLRTGFPGETAFTTGEAREALKTSRKHIVPLLEYLDAAGITQRQGDTRCMV

Specific function: Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP [H]

COG id: COG3276

COG function: function code J; Selenocysteine-specific translation elongation factor

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. SelB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=384, Percent_Identity=28.125, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI54607086, Length=382, Percent_Identity=24.869109947644, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI5729864, Length=303, Percent_Identity=28.3828382838284, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI223555963, Length=303, Percent_Identity=28.3828382838284, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI194018520, Length=307, Percent_Identity=28.9902280130293, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI194097354, Length=307, Percent_Identity=28.9902280130293, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI194018522, Length=307, Percent_Identity=28.9902280130293, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI46094014, Length=304, Percent_Identity=26.9736842105263, Blast_Score=89, Evalue=2e-17,
Organism=Escherichia coli, GI2367247, Length=625, Percent_Identity=28.64, Blast_Score=176, Evalue=5e-45,
Organism=Escherichia coli, GI1790412, Length=371, Percent_Identity=27.7628032345013, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789737, Length=371, Percent_Identity=27.7628032345013, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788922, Length=173, Percent_Identity=31.2138728323699, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=395, Percent_Identity=26.5822784810127, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI25141371, Length=376, Percent_Identity=27.9255319148936, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17552884, Length=316, Percent_Identity=24.3670886075949, Blast_Score=89, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17569207, Length=316, Percent_Identity=24.3670886075949, Blast_Score=89, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17506081, Length=254, Percent_Identity=24.4094488188976, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=369, Percent_Identity=25.2032520325203, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325337, Length=330, Percent_Identity=24.8484848484848, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319594, Length=330, Percent_Identity=24.8484848484848, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19921738, Length=353, Percent_Identity=30.5949008498584, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=27.7777777777778, Blast_Score=116, Evalue=6e-26,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=27.7777777777778, Blast_Score=116, Evalue=6e-26,
Organism=Drosophila melanogaster, GI19922690, Length=301, Percent_Identity=27.906976744186, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17137380, Length=312, Percent_Identity=25.9615384615385, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45550900, Length=190, Percent_Identity=32.6315789473684, Blast_Score=82, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015190
- InterPro:   IPR015191
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- InterPro:   IPR004535
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF09106 SelB-wing_2; PF09107 SelB-wing_3 [H]

EC number: NA

Molecular weight: Translated: 64225; Mature: 64225

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00137 SUBTILASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSCPVIVIGHVDHGKTSLVGALTGIETDRLPEEKARGLSITSGFAYLKGGDANIDLVDA
CCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHCCCEEECCEEEEECCCCCEEEEEC
PGHQNFIRAMVGGAAGARSAALVVSAAEGVEAQTLEHIAVIETLGIHAGIVVLSKADLVA
CCCHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCC
PSERPGRKVALQAALAGTAFRDAPLIFCSAVTGEGLEDIAAALRGLAALPAGAGPAGAFL
CCCCCCCCEEEHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHE
AIDRVFVAEGHGTVVTGTLLGRSLKPADELLLAPSGEIVTIRRIEVRGETVSMAQPGERT
EEEEEEEECCCCCEEEHHHHCCCCCCHHHEEECCCCCEEEEEEEEECCCEEECCCCCCCC
ALNLRGVAASKIKPGDVVHAADAGAASLNLDAAISVSARAGRPVRHMEEIRVLYGTGNGV
EEEECCCCCCCCCCCCEEEECCCCCCEECCCEEEEEECCCCCCHHHHHEEEEEEECCCCE
ATLRLLGRKEVSPGETGFAQLRFTAPAAAFAGQRAVLRSLSPSQTLGGAIILDPAATPLT
EEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCHHCCCEEEECCCCCCCC
ASKALRLATLEAAYRGDTLALAAALAAEQGGVARLGDVCRLARAPEASVRASLGEAFTQI
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ADGFVSPAAVVAEARDAYLARLCAYHAEHRLRPQAPRSEILAGHVSLLLNLHVEESLAAA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECHHHHHHHC
GVIRLSGGGVALAIHDAAANLTPDQTARMHGLARQLQADGLTPSGTAALIRSPEDAELLE
CEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHH
LLVERGEVIRLSNVSLKQTIWLHAEAVSSAIQMLRTGFPGETAFTTGEAREALKTSRKHI
HHHCCCCEEEEECCCEEHEEEEEHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHH
VPLLEYLDAAGITQRQGDTRCMV
HHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSCPVIVIGHVDHGKTSLVGALTGIETDRLPEEKARGLSITSGFAYLKGGDANIDLVDA
CCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHHCCCEEECCEEEEECCCCCEEEEEC
PGHQNFIRAMVGGAAGARSAALVVSAAEGVEAQTLEHIAVIETLGIHAGIVVLSKADLVA
CCCHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCC
PSERPGRKVALQAALAGTAFRDAPLIFCSAVTGEGLEDIAAALRGLAALPAGAGPAGAFL
CCCCCCCCEEEHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHE
AIDRVFVAEGHGTVVTGTLLGRSLKPADELLLAPSGEIVTIRRIEVRGETVSMAQPGERT
EEEEEEEECCCCCEEEHHHHCCCCCCHHHEEECCCCCEEEEEEEEECCCEEECCCCCCCC
ALNLRGVAASKIKPGDVVHAADAGAASLNLDAAISVSARAGRPVRHMEEIRVLYGTGNGV
EEEECCCCCCCCCCCCEEEECCCCCCEECCCEEEEEECCCCCCHHHHHEEEEEEECCCCE
ATLRLLGRKEVSPGETGFAQLRFTAPAAAFAGQRAVLRSLSPSQTLGGAIILDPAATPLT
EEEEEECCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCHHCCCEEEECCCCCCCC
ASKALRLATLEAAYRGDTLALAAALAAEQGGVARLGDVCRLARAPEASVRASLGEAFTQI
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ADGFVSPAAVVAEARDAYLARLCAYHAEHRLRPQAPRSEILAGHVSLLLNLHVEESLAAA
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECHHHHHHHC
GVIRLSGGGVALAIHDAAANLTPDQTARMHGLARQLQADGLTPSGTAALIRSPEDAELLE
CEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHH
LLVERGEVIRLSNVSLKQTIWLHAEAVSSAIQMLRTGFPGETAFTTGEAREALKTSRKHI
HHHCCCCEEEEECCCEEHEEEEEHHHHHHHHHHHHCCCCCCCCEECHHHHHHHHHHHHHH
VPLLEYLDAAGITQRQGDTRCMV
HHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8893853 [H]