Click here to switch to the map view.

The map label for this gene is lpdA1 [H]

Identifier: 114797424

GI number: 114797424

Start: 2045536

End: 2046951

Strand: Reverse

Name: lpdA1 [H]

Synonym: HNE_1974

Alternate gene names: 114797424

Gene position: 2046951-2045536 (Counterclockwise)

Preceding gene: 114798189

Following gene: 114797921

Centisome position: 55.25

GC content: 60.1

Gene sequence:

>1416_bases
ATGTCCACCCAATACGACCTCATCGTCATCGGCTCCGGTCCGGGCGGCTATGTCGCGGCGATCCGCGCGAGCCAGCTCGG
CATGAAGACAGCCATTGTGGAGCGCGAGAGCCTCGGCGGCATCTGTCTCAACTGGGGCTGCATCCCGACCAAGGCGCTGC
TTCGCTCGGCCGAAGTGCTACATCTGGCCAAGCACGCCAAATCCTTCGGCCTGAAAATCGAGAAGCCGGAATTTGATCTG
GAGGCCATCGTGAAACGCTCGCGCGGCATCGCGGCGCAGCTTTCGGGCGGCGTCAAATTCCTGATGAAGAAAAACAAGAT
CGACGTGATCGAGGGCGCAGCCCGGCTCGAGAAAGGCGCGCCCGCGCCCAAGGTTATCGTCAAGGGCAAGGATGGCAAGG
ATACGCCGTATACAGCCAAACATGTCATGCTCGCCACAGGCGCGCGCGCCCGCGATATTCCGCAGGCTGGCTTGAAAGCT
GACGGCAAGCTTGTTTGGACCTATCGCGAAGCGATGACGCCGGATGTGATGCCCAAACGCCTGCTCGTCATTGGATCGGG
CGCCATCGGCATCGAGTTTGCGAGCTTTTATAATGAGCTGGGCAGCGACGTGACCGTCGCCGAAGTGATGGACCGCATCC
TGCCCGTCGAAGACGCCGAAATCTCCGGCCTCGCCGAGAAGGATTTCAAGAAACAGGGCCTGAACATCCTCACCGGCGCC
AAGGTAGAAAACGTAAAGCCCGGCAAGAACACCGTCACGGCCGACATCACGACCAAGGATGGCAAGAAGGAAACGAAGGA
ATTCGACCGCGTCATCCTCGCCGTCGGCATCGTCGGCAATGTGGAAAATCTAGGCCTCGAAACGCTCGGCGTGAAGGTGG
AGAAAACCCATGTCGTGGTCGATGGCTTCGGGCGCACAGGCGTGCCCGGCCTCTATGCGATCGGCGATCTCACCGGCCCG
CCCTGGCTTGCCCACAAGGCCAGCCATGAGGGCGTGATGTGCGTTGAAGGCATCCATGGGAAAAACCATGCCGAGCCCTT
TGACGCCTCAAATATTCCGGGCTGCACCTATTCCCATCCCCAAATCGCCAGCGTTGGGCTCACAGAGGCCGCCGCGAAAG
AGAAGGGCTATGACATCAAGGTTGGCCGCTTTCCTTTCTTGGGCAATGGCAAGGCAATTGCTCTGGGGGCGGAGAATGGC
CTCGTCAAAACCATCTTCGACAAGAAGACCGGCGAGTTGCTGGGCGCCCACATGATCGGCGCCGAAGTTACCGAGCTGAT
CCAGGGCTATGTCATCGCCCGCCAGGGCGAGCTGACGGAAGCGGATCTGATCCACACCGTCTTCCCACACCCCACGCTCT
CGGAAACGATGCATGAAGCGGTTCTGGATGCAGAAGGCCGTGTGCTGCACATCTAG

Upstream 100 bases:

>100_bases
CGGCGGCGCAGAGGGCGCCAAATGGCTGACGGCGTTCAAGCGCTATGTGGAAACGCCCGAAGCGATGCTGCTTTAGCTGG
TGAATAAGAGGATCTAGGAC

Downstream 100 bases:

>100_bases
CCTTCTCACCTCGACTGCGCTTCGCGGCCCTCACCTCCCACCGCCAAAGGCGGTGGGAGGTGAGGGCCGCCCGAAGGGCG
CCCGCCTCAGAAAGTATACC

Product: pyruvate dehydrogenase complex, E3 component, dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSTQYDLIVIGSGPGGYVAAIRASQLGMKTAIVERESLGGICLNWGCIPTKALLRSAEVLHLAKHAKSFGLKIEKPEFDL
EAIVKRSRGIAAQLSGGVKFLMKKNKIDVIEGAARLEKGAPAPKVIVKGKDGKDTPYTAKHVMLATGARARDIPQAGLKA
DGKLVWTYREAMTPDVMPKRLLVIGSGAIGIEFASFYNELGSDVTVAEVMDRILPVEDAEISGLAEKDFKKQGLNILTGA
KVENVKPGKNTVTADITTKDGKKETKEFDRVILAVGIVGNVENLGLETLGVKVEKTHVVVDGFGRTGVPGLYAIGDLTGP
PWLAHKASHEGVMCVEGIHGKNHAEPFDASNIPGCTYSHPQIASVGLTEAAAKEKGYDIKVGRFPFLGNGKAIALGAENG
LVKTIFDKKTGELLGAHMIGAEVTELIQGYVIARQGELTEADLIHTVFPHPTLSETMHEAVLDAEGRVLHI

Sequences:

>Translated_471_residues
MSTQYDLIVIGSGPGGYVAAIRASQLGMKTAIVERESLGGICLNWGCIPTKALLRSAEVLHLAKHAKSFGLKIEKPEFDL
EAIVKRSRGIAAQLSGGVKFLMKKNKIDVIEGAARLEKGAPAPKVIVKGKDGKDTPYTAKHVMLATGARARDIPQAGLKA
DGKLVWTYREAMTPDVMPKRLLVIGSGAIGIEFASFYNELGSDVTVAEVMDRILPVEDAEISGLAEKDFKKQGLNILTGA
KVENVKPGKNTVTADITTKDGKKETKEFDRVILAVGIVGNVENLGLETLGVKVEKTHVVVDGFGRTGVPGLYAIGDLTGP
PWLAHKASHEGVMCVEGIHGKNHAEPFDASNIPGCTYSHPQIASVGLTEAAAKEKGYDIKVGRFPFLGNGKAIALGAENG
LVKTIFDKKTGELLGAHMIGAEVTELIQGYVIARQGELTEADLIHTVFPHPTLSETMHEAVLDAEGRVLHI
>Mature_470_residues
STQYDLIVIGSGPGGYVAAIRASQLGMKTAIVERESLGGICLNWGCIPTKALLRSAEVLHLAKHAKSFGLKIEKPEFDLE
AIVKRSRGIAAQLSGGVKFLMKKNKIDVIEGAARLEKGAPAPKVIVKGKDGKDTPYTAKHVMLATGARARDIPQAGLKAD
GKLVWTYREAMTPDVMPKRLLVIGSGAIGIEFASFYNELGSDVTVAEVMDRILPVEDAEISGLAEKDFKKQGLNILTGAK
VENVKPGKNTVTADITTKDGKKETKEFDRVILAVGIVGNVENLGLETLGVKVEKTHVVVDGFGRTGVPGLYAIGDLTGPP
WLAHKASHEGVMCVEGIHGKNHAEPFDASNIPGCTYSHPQIASVGLTEAAAKEKGYDIKVGRFPFLGNGKAIALGAENGL
VKTIFDKKTGELLGAHMIGAEVTELIQGYVIARQGELTEADLIHTVFPHPTLSETMHEAVLDAEGRVLHI

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=35.6837606837607, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=30, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI22035672, Length=479, Percent_Identity=28.1837160751566, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI291045266, Length=437, Percent_Identity=27.2311212814645, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI148277065, Length=479, Percent_Identity=28.1837160751566, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI33519430, Length=479, Percent_Identity=28.1837160751566, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI33519428, Length=479, Percent_Identity=28.1837160751566, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI33519426, Length=479, Percent_Identity=28.1837160751566, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI148277071, Length=479, Percent_Identity=28.1837160751566, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI291045268, Length=481, Percent_Identity=26.1954261954262, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786307, Length=472, Percent_Identity=36.0169491525424, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1789915, Length=466, Percent_Identity=30.9012875536481, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI87082354, Length=478, Percent_Identity=27.4058577405858, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=26.4957264957265, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI32565766, Length=464, Percent_Identity=37.2844827586207, Blast_Score=290, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI17557007, Length=480, Percent_Identity=26.875, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71983429, Length=467, Percent_Identity=28.051391862955, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=467, Percent_Identity=28.051391862955, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=492, Percent_Identity=26.8292682926829, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=481, Percent_Identity=36.1746361746362, Blast_Score=259, Evalue=5e-70,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=28.9029535864979, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=28.6315789473684, Blast_Score=170, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21358499, Length=467, Percent_Identity=38.3297644539615, Blast_Score=288, Evalue=7e-78,
Organism=Drosophila melanogaster, GI24640549, Length=483, Percent_Identity=29.8136645962733, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640553, Length=483, Percent_Identity=29.8136645962733, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24640551, Length=483, Percent_Identity=29.8136645962733, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17737741, Length=482, Percent_Identity=28.2157676348548, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50172; Mature: 50041

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQYDLIVIGSGPGGYVAAIRASQLGMKTAIVERESLGGICLNWGCIPTKALLRSAEVL
CCCCEEEEEEECCCCCEEEEEEHHHCCHHHHEEEHHCCCCEEEECCCCCHHHHHHHHHHH
HLAKHAKSFGLKIEKPEFDLEAIVKRSRGIAAQLSGGVKFLMKKNKIDVIEGAARLEKGA
HHHHHHHHHCCEEECCCCCHHHHHHHHCCCEEECCCCEEEEEECCCCCCHHHHHHHHCCC
PAPKVIVKGKDGKDTPYTAKHVMLATGARARDIPQAGLKADGKLVWTYREAMTPDVMPKR
CCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCE
LLVIGSGAIGIEFASFYNELGSDVTVAEVMDRILPVEDAEISGLAEKDFKKQGLNILTGA
EEEEECCCHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEECC
KVENVKPGKNTVTADITTKDGKKETKEFDRVILAVGIVGNVENLGLETLGVKVEKTHVVV
EECCCCCCCCEEEEEEECCCCCHHHHHHHHEEEEEEEECCHHHCCEEHHCEEEEEEEEEE
DGFGRTGVPGLYAIGDLTGPPWLAHKASHEGVMCVEGIHGKNHAEPFDASNIPGCTYSHP
ECCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
QIASVGLTEAAAKEKGYDIKVGRFPFLGNGKAIALGAENGLVKTIFDKKTGELLGAHMIG
CEEECCCHHHHHHCCCCEEEECCCCEECCCCEEEEECCCCEEEEECCCCHHHHHHHHHHH
AEVTELIQGYVIARQGELTEADLIHTVFPHPTLSETMHEAVLDAEGRVLHI
HHHHHHHHCEEEEECCCCCHHHHHHEECCCCCHHHHHHHHHHCCCCCEEEC
>Mature Secondary Structure 
STQYDLIVIGSGPGGYVAAIRASQLGMKTAIVERESLGGICLNWGCIPTKALLRSAEVL
CCCEEEEEEECCCCCEEEEEEHHHCCHHHHEEEHHCCCCEEEECCCCCHHHHHHHHHHH
HLAKHAKSFGLKIEKPEFDLEAIVKRSRGIAAQLSGGVKFLMKKNKIDVIEGAARLEKGA
HHHHHHHHHCCEEECCCCCHHHHHHHHCCCEEECCCCEEEEEECCCCCCHHHHHHHHCCC
PAPKVIVKGKDGKDTPYTAKHVMLATGARARDIPQAGLKADGKLVWTYREAMTPDVMPKR
CCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCCE
LLVIGSGAIGIEFASFYNELGSDVTVAEVMDRILPVEDAEISGLAEKDFKKQGLNILTGA
EEEEECCCHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEECC
KVENVKPGKNTVTADITTKDGKKETKEFDRVILAVGIVGNVENLGLETLGVKVEKTHVVV
EECCCCCCCCEEEEEEECCCCCHHHHHHHHEEEEEEEECCHHHCCEEHHCEEEEEEEEEE
DGFGRTGVPGLYAIGDLTGPPWLAHKASHEGVMCVEGIHGKNHAEPFDASNIPGCTYSHP
ECCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
QIASVGLTEAAAKEKGYDIKVGRFPFLGNGKAIALGAENGLVKTIFDKKTGELLGAHMIG
CEEECCCHHHHHHCCCCEEEECCCCEECCCCEEEEECCCCEEEEECCCCHHHHHHHHHHH
AEVTELIQGYVIARQGELTEADLIHTVFPHPTLSETMHEAVLDAEGRVLHI
HHHHHHHHCEEEEECCCCCHHHHHHEECCCCCHHHHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]