Click here to switch to the map view.

The map label for this gene is trmB

Identifier: 114798099

GI number: 114798099

Start: 97510

End: 98169

Strand: Direct

Name: trmB

Synonym: HNE_0109

Alternate gene names: 114798099

Gene position: 97510-98169 (Clockwise)

Preceding gene: 114799525

Following gene: 114797127

Centisome position: 2.63

GC content: 61.36

Gene sequence:

>660_bases
TTGCGCAGCTTTGGCCGGACAGGCGGGCGCCCCCTGTCGCCGCGTCAGAAAACCCTGATGGCGGAGAAGCTGGATACCTA
CCGTATTGCTGAGGGCGACGGGCCCTTAGATCTGTGCGCAATGTTTCCCGGAAAAGAGGCTTTCTGGTTGGAAATCGGCT
TCGGCGGCGCTGAACATATGATCGCTCAGGCGGCGCAGAATCCAGATGTCGGCATCATTGGCTGCGAGCCCTTTCTGGAA
GGGATCGCCAAGGCTCTGGGGGGGATCGAAGAGACGGACCTGAATAATGTCCGCGTCTGGCCAGACGATGCCCGGCCGCT
GATCGCCCGCATGCCGGACCAGTGCCTCGATCGCGCCTTCGTCCTTTTCCCGGACCCATGGCCCAAGCGCCGGCAGCACA
AGCGCCGCCTTGTGCAGGCAGACTTTCTGTCCATTCTGCATCCAAAAATGAAACCCGGAGCGCGCCTGCGCTTTGCAACC
GACGTCAAAAGCTATGCCGACGAGGCGCTGCTGACGTTCGTATCCGACGGCCGGTTTGAATGGCTGGCCGCTTCGCCGGA
TGATTGGCGCTGCCCGCCGCAGGATCATGTGACCACCCGCTACGAGTCCAAGCGGCTTGGCGACTGCAAACCGGTATGGT
TTGACTTCCGGATACGTTGA

Upstream 100 bases:

>100_bases
GCGACTGTGCGTGCGGCGGCCTCAGCATTTGACACCCGCGCCAATTCCGACCGGAGCCGGGATGACTGACACCAGAGATT
CAGAGTTCCGGGATCGCCCA

Downstream 100 bases:

>100_bases
TCTGATGGGGCTTCTTGACGAAAACGCCCGTACATGCACAAATCTAGGCTATCGAAACCGGTCGGTCTGAATATCAAGAC
CCCGTCAGACCGACGGCAAT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MRSFGRTGGRPLSPRQKTLMAEKLDTYRIAEGDGPLDLCAMFPGKEAFWLEIGFGGAEHMIAQAAQNPDVGIIGCEPFLE
GIAKALGGIEETDLNNVRVWPDDARPLIARMPDQCLDRAFVLFPDPWPKRRQHKRRLVQADFLSILHPKMKPGARLRFAT
DVKSYADEALLTFVSDGRFEWLAASPDDWRCPPQDHVTTRYESKRLGDCKPVWFDFRIR

Sequences:

>Translated_219_residues
MRSFGRTGGRPLSPRQKTLMAEKLDTYRIAEGDGPLDLCAMFPGKEAFWLEIGFGGAEHMIAQAAQNPDVGIIGCEPFLE
GIAKALGGIEETDLNNVRVWPDDARPLIARMPDQCLDRAFVLFPDPWPKRRQHKRRLVQADFLSILHPKMKPGARLRFAT
DVKSYADEALLTFVSDGRFEWLAASPDDWRCPPQDHVTTRYESKRLGDCKPVWFDFRIR
>Mature_219_residues
MRSFGRTGGRPLSPRQKTLMAEKLDTYRIAEGDGPLDLCAMFPGKEAFWLEIGFGGAEHMIAQAAQNPDVGIIGCEPFLE
GIAKALGGIEETDLNNVRVWPDDARPLIARMPDQCLDRAFVLFPDPWPKRRQHKRRLVQADFLSILHPKMKPGARLRFAT
DVKSYADEALLTFVSDGRFEWLAASPDDWRCPPQDHVTTRYESKRLGDCKPVWFDFRIR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=210, Percent_Identity=31.9047619047619, Blast_Score=98, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_HYPNA (Q0C600)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_758843.1
- ProteinModelPortal:   Q0C600
- SMR:   Q0C600
- STRING:   Q0C600
- GeneID:   4288247
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0109
- NMPDR:   fig|228405.5.peg.107
- TIGR:   HNE_0109
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   RYGRKAE
- PhylomeDB:   Q0C600
- ProtClustDB:   PRK00121
- BioCyc:   HNEP81032:HNE_0109-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 24900; Mature: 24900

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: BINDING 51-51 BINDING 76-76 BINDING 103-103 BINDING 125-125 BINDING 129-129 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFGRTGGRPLSPRQKTLMAEKLDTYRIAEGDGPLDLCAMFPGKEAFWLEIGFGGAEHM
CCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEECCCCCEEEEEECCCCHHHH
IAQAAQNPDVGIIGCEPFLEGIAKALGGIEETDLNNVRVWPDDARPLIARMPDQCLDRAF
HHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHCCCHHHHCCEE
VLFPDPWPKRRQHKRRLVQADFLSILHPKMKPGARLRFATDVKSYADEALLTFVSDGRFE
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEE
WLAASPDDWRCPPQDHVTTRYESKRLGDCKPVWFDFRIR
EEECCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MRSFGRTGGRPLSPRQKTLMAEKLDTYRIAEGDGPLDLCAMFPGKEAFWLEIGFGGAEHM
CCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCEEEEEECCCCCEEEEEECCCCHHHH
IAQAAQNPDVGIIGCEPFLEGIAKALGGIEETDLNNVRVWPDDARPLIARMPDQCLDRAF
HHHHHCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHCCCHHHHCCEE
VLFPDPWPKRRQHKRRLVQADFLSILHPKMKPGARLRFATDVKSYADEALLTFVSDGRFE
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEE
WLAASPDDWRCPPQDHVTTRYESKRLGDCKPVWFDFRIR
EEECCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA