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The map label for this gene is glxR [C]

Identifier: 114797994

GI number: 114797994

Start: 913961

End: 914827

Strand: Direct

Name: glxR [C]

Synonym: HNE_0895

Alternate gene names: 114797994

Gene position: 913961-914827 (Clockwise)

Preceding gene: 114799958

Following gene: 114797056

Centisome position: 24.67

GC content: 66.78

Gene sequence:

>867_bases
ATGGCGCGCACGGCATTTCTCGGCCTTGGGGTAATGGGCTTCCATATGGCGGGCCATCTTGCGCGGGCGGGCCATCAGGT
CGCCGTCTGGAACCGCTCCCCGGCCAAATCGGCGGCCTGGACCGGCGTTCACCGCGGCGAAGCGGCCAAAGACCCCGCGT
CTGCCGTCTTCGGCGCCGAATATGTTTTGCTGTGCCTCGGCGATGATCCCGATGTCCGCGCCGTGTTCGACGCCTTCGAG
CCGAGCCTCGGCGCGGGCATGACCGTCATCGACCACACCACCGCCTCTGCCGCGCTTGCCCGCGAACTGGCCGAGCGCTG
CCGCGCCAAGGGCGCCCATTTCATCGACGCGCCGGTTTCGGGCGGCGAGGCCGGCGCCATCAATGGCAAGCTCACCATCA
TGTGCGGCGGGGAGGAAGCCCCCTTCGCCAAGGCCGAGCCCGTCATGAACGCCTTTGCCCGCGCGATCACCCTGATCGGC
CCCAGCGGCGCGGGCCAGCTTGCCAAATCAGTCAACCAGATCTGCATCGCCGGCATCGTCCAGGGCCTCGCCGAAGGGCT
CCACTTTGCCGAGAAGGCGGGTCTCGACGCGGAGAAAGTCATCGCCGCCATTTCCGGCGGCGCCGCCCAGAGCTGGCAGA
TGGAAAACCGCTGGAAGACGATGACCGACGGAAAATTCGATTTCGGCTTCGCGGTTGACTGGATGCGCAAAGATCTCCGC
ATCACGCTGGATGCCGCCCGCGAAAACGGCGCAAGCCTTCCCCTCACGGCCCAGGTCGATCAATACTATGCCGACGTCCA
GGCCATGGGCGGCAACCGCTGGGACACGTCCAGCCTCATCGCCCGCCTGGGCAGAAAGCCGGACTGA

Upstream 100 bases:

>100_bases
CGCAGGGTGTATCAGGTCGTGGTGAAAGCCACGCTTCGGCCAGGGGTGGGATTTCGATGAAATTCGCTCTAATTGGCCTT
GAATTCATAAGGAGACTGGC

Downstream 100 bases:

>100_bases
TGGGTATTTGCCGCGTCGACATCCACATTCCCGCTGATGGCGAGGTGTATGATACTGTAATGAGGATCGTCAAAGCGGCA
GACCCGATCGACTACCGGGT

Product: 3-hydroxyisobutyrate dehydrogenase family protein

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MARTAFLGLGVMGFHMAGHLARAGHQVAVWNRSPAKSAAWTGVHRGEAAKDPASAVFGAEYVLLCLGDDPDVRAVFDAFE
PSLGAGMTVIDHTTASAALARELAERCRAKGAHFIDAPVSGGEAGAINGKLTIMCGGEEAPFAKAEPVMNAFARAITLIG
PSGAGQLAKSVNQICIAGIVQGLAEGLHFAEKAGLDAEKVIAAISGGAAQSWQMENRWKTMTDGKFDFGFAVDWMRKDLR
ITLDAARENGASLPLTAQVDQYYADVQAMGGNRWDTSSLIARLGRKPD

Sequences:

>Translated_288_residues
MARTAFLGLGVMGFHMAGHLARAGHQVAVWNRSPAKSAAWTGVHRGEAAKDPASAVFGAEYVLLCLGDDPDVRAVFDAFE
PSLGAGMTVIDHTTASAALARELAERCRAKGAHFIDAPVSGGEAGAINGKLTIMCGGEEAPFAKAEPVMNAFARAITLIG
PSGAGQLAKSVNQICIAGIVQGLAEGLHFAEKAGLDAEKVIAAISGGAAQSWQMENRWKTMTDGKFDFGFAVDWMRKDLR
ITLDAARENGASLPLTAQVDQYYADVQAMGGNRWDTSSLIARLGRKPD
>Mature_287_residues
ARTAFLGLGVMGFHMAGHLARAGHQVAVWNRSPAKSAAWTGVHRGEAAKDPASAVFGAEYVLLCLGDDPDVRAVFDAFEP
SLGAGMTVIDHTTASAALARELAERCRAKGAHFIDAPVSGGEAGAINGKLTIMCGGEEAPFAKAEPVMNAFARAITLIGP
SGAGQLAKSVNQICIAGIVQGLAEGLHFAEKAGLDAEKVIAAISGGAAQSWQMENRWKTMTDGKFDFGFAVDWMRKDLRI
TLDAARENGASLPLTAQVDQYYADVQAMGGNRWDTSSLIARLGRKPD

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=28.1786941580756, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI40556376, Length=288, Percent_Identity=23.9583333333333, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786719, Length=285, Percent_Identity=34.7368421052632, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=33.4507042253521, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790315, Length=288, Percent_Identity=29.1666666666667, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1789092, Length=281, Percent_Identity=27.7580071174377, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788341, Length=205, Percent_Identity=27.8048780487805, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557316, Length=274, Percent_Identity=27.3722627737226, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24655230, Length=283, Percent_Identity=30.3886925795053, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922568, Length=283, Percent_Identity=30.3886925795053, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28574115, Length=284, Percent_Identity=23.5915492957746, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30257; Mature: 30125

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTAFLGLGVMGFHMAGHLARAGHQVAVWNRSPAKSAAWTGVHRGEAAKDPASAVFGAE
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCCCCCHHHHHHCCE
YVLLCLGDDPDVRAVFDAFEPSLGAGMTVIDHTTASAALARELAERCRAKGAHFIDAPVS
EEEEEECCCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEECCCC
GGEAGAINGKLTIMCGGEEAPFAKAEPVMNAFARAITLIGPSGAGQLAKSVNQICIAGIV
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHH
QGLAEGLHFAEKAGLDAEKVIAAISGGAAQSWQMENRWKTMTDGKFDFGFAVDWMRKDLR
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCE
ITLDAARENGASLPLTAQVDQYYADVQAMGGNRWDTSSLIARLGRKPD
EEEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
ARTAFLGLGVMGFHMAGHLARAGHQVAVWNRSPAKSAAWTGVHRGEAAKDPASAVFGAE
CCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHCCCCCCCCCCCHHHHHHCCE
YVLLCLGDDPDVRAVFDAFEPSLGAGMTVIDHTTASAALARELAERCRAKGAHFIDAPVS
EEEEEECCCCCHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCEEEECCCC
GGEAGAINGKLTIMCGGEEAPFAKAEPVMNAFARAITLIGPSGAGQLAKSVNQICIAGIV
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHH
QGLAEGLHFAEKAGLDAEKVIAAISGGAAQSWQMENRWKTMTDGKFDFGFAVDWMRKDLR
HHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCE
ITLDAARENGASLPLTAQVDQYYADVQAMGGNRWDTSSLIARLGRKPD
EEEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA