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The map label for this gene is ycdT [H]

Identifier: 114797892

GI number: 114797892

Start: 2029370

End: 2030245

Strand: Reverse

Name: ycdT [H]

Synonym: HNE_1960

Alternate gene names: 114797892

Gene position: 2030245-2029370 (Counterclockwise)

Preceding gene: 114798448

Following gene: 114798700

Centisome position: 54.8

GC content: 57.88

Gene sequence:

>876_bases
ATGACAATTTCTCGCCGTGACATCTGGAGCGCCGTGAATTTCTTCATGCTCGTTGCCTGCACGGCTCTTTCTTTGTCGTT
CACTGTTTCGCTGTTGTTCTACAAAATGGGTTTGCCGACCGATATCAGCTCGTATGTCCGGCTGAACCTGTTCGTCTCGG
GCTGCGTCGCACTGCCGACTGCGATCATCGCTTCTCTTCATGATTTCCATATGCGCGCATATCAGCGCCGGCTTGAAGAG
ATGGCGTGGACCGATGCCCTCACCGGCCTCCTGAACCGGCGTTTCTTCATCCATGCCGCCGAAGAAGAGCGCTCCCGCAT
GCGCCGCACCAAGCAGAGCGCCGCTATTGTTCTAATCGATCTCGACCACTTCAAGGAAGTAAATGACCTTTACGGCCATT
CCGGTGGAGACCTCGTCCTCAAGGAAATCGCCGCCATTGCCCACTCCGAGCTGCGCGGACCATTTGATCGCCTCGGTCGC
TGGGGTGGCGAAGAATTTGTCGTCCTGCTCAGCTGCGTGAGCAAGGAACATGCGATGATTGTCTGTGAGCGTCTGCGCCG
GCGCATTGAAGCGGCAACTATATTTGTGGGCGATAAGGAAGTTTCCGTTACCGCCAGCTTCGGCCTCTGTATGATGAGCC
CTGAGACGCCGCTCAACGCCGCGATCGATTGCGCTGATGGCGCGCTCTATGAGGTCAAACGTCTCGGACGTAACAAGGTT
CTCTTCCGGGATGTGCCTGAAGGAGAAGCCGCCCCCATCGTTACTAGCCTCCCGATACGGCGCCGCTTCGGGCAGCGCAT
ACCGCAAAATGCGGTCGTGCAGCCACCCGCCAATGACGGTGCCGGGGAGCCTGTGCGCAAAGCTGCGTCCAGCTGA

Upstream 100 bases:

>100_bases
TTGGCTTGATCGCAGCCTGTCAACTGAAGGTCGCGCGAAAGGTCGAAGTCAAAACTATTGCGATAGGTTTGCAGACATCA
GCCTGCCGGAGCCGCTTTCA

Downstream 100 bases:

>100_bases
TCTACCGCTAACCCTTCCTGTAGCTTGCCGCCCCGCAGATTTCCTGATTGGAACGCAGGGCCGCATCCGGGAGGAACACC
ATGCCATCATTGCCGCCCCG

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MTISRRDIWSAVNFFMLVACTALSLSFTVSLLFYKMGLPTDISSYVRLNLFVSGCVALPTAIIASLHDFHMRAYQRRLEE
MAWTDALTGLLNRRFFIHAAEEERSRMRRTKQSAAIVLIDLDHFKEVNDLYGHSGGDLVLKEIAAIAHSELRGPFDRLGR
WGGEEFVVLLSCVSKEHAMIVCERLRRRIEAATIFVGDKEVSVTASFGLCMMSPETPLNAAIDCADGALYEVKRLGRNKV
LFRDVPEGEAAPIVTSLPIRRRFGQRIPQNAVVQPPANDGAGEPVRKAASS

Sequences:

>Translated_291_residues
MTISRRDIWSAVNFFMLVACTALSLSFTVSLLFYKMGLPTDISSYVRLNLFVSGCVALPTAIIASLHDFHMRAYQRRLEE
MAWTDALTGLLNRRFFIHAAEEERSRMRRTKQSAAIVLIDLDHFKEVNDLYGHSGGDLVLKEIAAIAHSELRGPFDRLGR
WGGEEFVVLLSCVSKEHAMIVCERLRRRIEAATIFVGDKEVSVTASFGLCMMSPETPLNAAIDCADGALYEVKRLGRNKV
LFRDVPEGEAAPIVTSLPIRRRFGQRIPQNAVVQPPANDGAGEPVRKAASS
>Mature_290_residues
TISRRDIWSAVNFFMLVACTALSLSFTVSLLFYKMGLPTDISSYVRLNLFVSGCVALPTAIIASLHDFHMRAYQRRLEEM
AWTDALTGLLNRRFFIHAAEEERSRMRRTKQSAAIVLIDLDHFKEVNDLYGHSGGDLVLKEIAAIAHSELRGPFDRLGRW
GGEEFVVLLSCVSKEHAMIVCERLRRRIEAATIFVGDKEVSVTASFGLCMMSPETPLNAAIDCADGALYEVKRLGRNKVL
FRDVPEGEAAPIVTSLPIRRRFGQRIPQNAVVQPPANDGAGEPVRKAASS

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=256, Percent_Identity=32.8125, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=35.031847133758, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081881, Length=171, Percent_Identity=39.1812865497076, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786584, Length=232, Percent_Identity=29.3103448275862, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082007, Length=179, Percent_Identity=35.195530726257, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=33.9506172839506, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788085, Length=170, Percent_Identity=32.9411764705882, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081977, Length=157, Percent_Identity=34.3949044585987, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=34.8066298342541, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081974, Length=181, Percent_Identity=28.1767955801105, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787541, Length=175, Percent_Identity=33.1428571428571, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787802, Length=244, Percent_Identity=25.8196721311475, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788956, Length=166, Percent_Identity=30.1204819277108, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 32385; Mature: 32254

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISRRDIWSAVNFFMLVACTALSLSFTVSLLFYKMGLPTDISSYVRLNLFVSGCVALPT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AIIASLHDFHMRAYQRRLEEMAWTDALTGLLNRRFFIHAAEEERSRMRRTKQSAAIVLID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCEEEEEE
LDHFKEVNDLYGHSGGDLVLKEIAAIAHSELRGPFDRLGRWGGEEFVVLLSCVSKEHAMI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCHHHHH
VCERLRRRIEAATIFVGDKEVSVTASFGLCMMSPETPLNAAIDCADGALYEVKRLGRNKV
HHHHHHHHHHHHEEEECCCCEEEEEECCEEEECCCCCCCHHHCCCCCHHHHHHHCCCCCE
LFRDVPEGEAAPIVTSLPIRRRFGQRIPQNAVVQPPANDGAGEPVRKAASS
EEEECCCCCCCCEEECCHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
TISRRDIWSAVNFFMLVACTALSLSFTVSLLFYKMGLPTDISSYVRLNLFVSGCVALPT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AIIASLHDFHMRAYQRRLEEMAWTDALTGLLNRRFFIHAAEEERSRMRRTKQSAAIVLID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCEEEEEE
LDHFKEVNDLYGHSGGDLVLKEIAAIAHSELRGPFDRLGRWGGEEFVVLLSCVSKEHAMI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCHHHHH
VCERLRRRIEAATIFVGDKEVSVTASFGLCMMSPETPLNAAIDCADGALYEVKRLGRNKV
HHHHHHHHHHHHEEEECCCCEEEEEECCEEEECCCCCCCHHHCCCCCHHHHHHHCCCCCE
LFRDVPEGEAAPIVTSLPIRRRFGQRIPQNAVVQPPANDGAGEPVRKAASS
EEEECCCCCCCCEEECCHHHHHHHHHCCCCCEECCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]