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The map label for this gene is psdht [H]

Identifier: 114797836

GI number: 114797836

Start: 1159419

End: 1160414

Strand: Direct

Name: psdht [H]

Synonym: HNE_1148

Alternate gene names: 114797836

Gene position: 1159419-1160414 (Clockwise)

Preceding gene: 114799246

Following gene: 114797097

Centisome position: 31.29

GC content: 68.78

Gene sequence:

>996_bases
ATGACCCAGACCCTGACACTCCCCGCTTTTGAAGATGTTCTTGCCGCCGAGCAGCGCCAGCGCGGCGTCGTGCGCGAGAC
GCCTGTGCTGAGCCATCCGGCACTGGATGAGGCCGCCGGAGCGGAGCTGCTCATCAAGGCCGAATGCCTGCAGGTAACGG
GAAGTTTCAAGATCCGCGGGGCGATGAACCGGCTGGCGCAGGTGCCGCCGGAAGGCAAGGCGGCGGGCGTTGTGGCGTTC
TCCTCGGGCAACCATGCGCAGGGCGTGGCGCGGGCCGCGCGCCTGCTGGGGATGCCGGCGCTGATCGTGATGCCGTCGGA
CGCCCCGCAGGTGAAGGTGGACGGCGTGCTGGCCGATGGCGGCGAGGTTTACCTTTACGACCGCAACACCGAGAACCGCG
AAGAGATCTGCGCGCGGCTGGCGGCCGAACGCGGCGCGGCGATTGTGCCCAGCTATGATGATTTCGACATCATCGCCGGG
CAGGGCACGGCGGGCCTGGAGTTTGCGCGCCAGGCCGAGGCGATGGGCAAGCCGCTGGACCATCTGATTTCGCCGGCCGG
CGGGGGCGGGCTGATCAACGGCGTGGCGCTGGCCTTCGGGCAGCTTTCGCCCGCCACGAAAATCTGGGCGGCAGAGCCCG
AGGCGCATGACGACTGGGCGCGGTCTCTCGAAGCGGGGGAAATCCTCGCCAATGCGCCGGGAACACGCTCGATCTGTGAT
GCCATCCTGACACCGCAGCCGGGATACCTGACCTTTGCGCTGGGCCAGCGGCAGCTGGCGGGGGCCTTCCGGGTGTCGGA
TGAGGATGTGCGCGAGGCAATGCGGATGGCGTTTCGCTATCTGCGCGTGGTGGCCGAGCCGGGCGGGTCTGCGGCATTGG
CGGCCGCGCTGAGCGGGCTGCCGGAGGCCCTGAAGGGCAAACGGATCGGCATCATCGTTTCGGGCGGGAATGTGGACGCG
GCGCAGTATGCCGCCATTTTGGGCGCGGGCGCGTAG

Upstream 100 bases:

>100_bases
GGCTCTGGACGTGGCGGTGAAATATCCTGTCATATGAAGTGGGGGCGGCGGGTCTTTTGCCCGCTTTACCTCGCGTCGCG
GTTCTGGTTAGGACGGCGCC

Downstream 100 bases:

>100_bases
GGTGTGGTTTACGCGCGTTAACCAAGTCTGCTTACGGATAATAATTCGTGAAGCCGCCACAAGTATGGTTAAATTCACCT
TCCGCACCCGGAGGAGATTA

Product: pyridoxal-phosphate-dependent enzyme

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTQTLTLPAFEDVLAAEQRQRGVVRETPVLSHPALDEAAGAELLIKAECLQVTGSFKIRGAMNRLAQVPPEGKAAGVVAF
SSGNHAQGVARAARLLGMPALIVMPSDAPQVKVDGVLADGGEVYLYDRNTENREEICARLAAERGAAIVPSYDDFDIIAG
QGTAGLEFARQAEAMGKPLDHLISPAGGGGLINGVALAFGQLSPATKIWAAEPEAHDDWARSLEAGEILANAPGTRSICD
AILTPQPGYLTFALGQRQLAGAFRVSDEDVREAMRMAFRYLRVVAEPGGSAALAAALSGLPEALKGKRIGIIVSGGNVDA
AQYAAILGAGA

Sequences:

>Translated_331_residues
MTQTLTLPAFEDVLAAEQRQRGVVRETPVLSHPALDEAAGAELLIKAECLQVTGSFKIRGAMNRLAQVPPEGKAAGVVAF
SSGNHAQGVARAARLLGMPALIVMPSDAPQVKVDGVLADGGEVYLYDRNTENREEICARLAAERGAAIVPSYDDFDIIAG
QGTAGLEFARQAEAMGKPLDHLISPAGGGGLINGVALAFGQLSPATKIWAAEPEAHDDWARSLEAGEILANAPGTRSICD
AILTPQPGYLTFALGQRQLAGAFRVSDEDVREAMRMAFRYLRVVAEPGGSAALAAALSGLPEALKGKRIGIIVSGGNVDA
AQYAAILGAGA
>Mature_330_residues
TQTLTLPAFEDVLAAEQRQRGVVRETPVLSHPALDEAAGAELLIKAECLQVTGSFKIRGAMNRLAQVPPEGKAAGVVAFS
SGNHAQGVARAARLLGMPALIVMPSDAPQVKVDGVLADGGEVYLYDRNTENREEICARLAAERGAAIVPSYDDFDIIAGQ
GTAGLEFARQAEAMGKPLDHLISPAGGGGLINGVALAFGQLSPATKIWAAEPEAHDDWARSLEAGEILANAPGTRSICDA
ILTPQPGYLTFALGQRQLAGAFRVSDEDVREAMRMAFRYLRVVAEPGGSAALAAALSGLPEALKGKRIGIIVSGGNVDAA
QYAAILGAGA

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=317, Percent_Identity=34.7003154574132, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1789505, Length=330, Percent_Identity=33.6363636363636, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1790207, Length=294, Percent_Identity=36.734693877551, Blast_Score=135, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17508781, Length=323, Percent_Identity=30.9597523219814, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71991565, Length=312, Percent_Identity=33.3333333333333, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17537387, Length=313, Percent_Identity=33.8658146964856, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6322631, Length=324, Percent_Identity=43.2098765432099, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6320930, Length=318, Percent_Identity=32.0754716981132, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319788, Length=287, Percent_Identity=25.4355400696864, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21355833, Length=323, Percent_Identity=34.6749226006192, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 34345; Mature: 34213

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTLTLPAFEDVLAAEQRQRGVVRETPVLSHPALDEAAGAELLIKAECLQVTGSFKIRG
CCCEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCEEEEEEEEEHCCCCEEEHH
AMNRLAQVPPEGKAAGVVAFSSGNHAQGVARAARLLGMPALIVMPSDAPQVKVDGVLADG
HHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEECC
GEVYLYDRNTENREEICARLAAERGAAIVPSYDDFDIIAGQGTAGLEFARQAEAMGKPLD
CEEEEEECCCCCHHHHHHHHHHHCCCEECCCCCCCEEEECCCCCHHHHHHHHHHHCCCHH
HLISPAGGGGLINGVALAFGQLSPATKIWAAEPEAHDDWARSLEAGEILANAPGTRSICD
HHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHCCCHHEECCCCHHHHHH
AILTPQPGYLTFALGQRQLAGAFRVSDEDVREAMRMAFRYLRVVAEPGGSAALAAALSGL
HHCCCCCCEEEEECCCHHHHHHEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
PEALKGKRIGIIVSGGNVDAAQYAAILGAGA
HHHHCCCEEEEEEECCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TQTLTLPAFEDVLAAEQRQRGVVRETPVLSHPALDEAAGAELLIKAECLQVTGSFKIRG
CCEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCEEEEEEEEEHCCCCEEEHH
AMNRLAQVPPEGKAAGVVAFSSGNHAQGVARAARLLGMPALIVMPSDAPQVKVDGVLADG
HHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEEEEECC
GEVYLYDRNTENREEICARLAAERGAAIVPSYDDFDIIAGQGTAGLEFARQAEAMGKPLD
CEEEEEECCCCCHHHHHHHHHHHCCCEECCCCCCCEEEECCCCCHHHHHHHHHHHCCCHH
HLISPAGGGGLINGVALAFGQLSPATKIWAAEPEAHDDWARSLEAGEILANAPGTRSICD
HHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHCCCHHEECCCCHHHHHH
AILTPQPGYLTFALGQRQLAGAFRVSDEDVREAMRMAFRYLRVVAEPGGSAALAAALSGL
HHCCCCCCEEEEECCCHHHHHHEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
PEALKGKRIGIIVSGGNVDAAQYAAILGAGA
HHHHCCCEEEEEEECCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {L-Ser}} 5 {L-Ser}} 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]