Click here to switch to the map view.

The map label for this gene is queA

Identifier: 114797801

GI number: 114797801

Start: 2916630

End: 2917685

Strand: Reverse

Name: queA

Synonym: HNE_2757

Alternate gene names: 114797801

Gene position: 2917685-2916630 (Counterclockwise)

Preceding gene: 114800546

Following gene: 114798016

Centisome position: 78.75

GC content: 67.61

Gene sequence:

>1056_bases
ATGGACCTCTCGGTATTCCAGTTTGATCTGCCTGAAGATCTCATCGCCCTGCGGCCCGCCCAGCCGCAGGATGCCGCGCG
CCTGCTCGTGGTGCATGGCGACGGGCGCCTGATTGACGCCACCGTCCGGGATCTGCCGAAATATCTCAACACCGGCGACG
TCCTCGTCTTCAACGATACGCGCGTGCTGCCCGCCGCGCTCAAGGGCGTGCGCCCGGCGCGCGACGGGGCCAGCCGCGAT
GTCGAGGTCGATGTAAACCTGGTCGAGCGTCAGGGCGATTGTCTCTGGCTCGCGCTCGCCCGGCCCGGCCGCCGCCTGAA
GGATGGCGACGAGATTTTCTTCGCCGAAGGCTTTTCAGCCAGGATAGCGTCGCGCCGCGAAGGCGGGGAAATCCTGCTGG
ACTTCAACTGCGAGGGCGAAGCGATGATCGCCGCGCTCGACGCCCACGGGGCCATGCCGCTGCCGCCCTATATCGCCCGC
CGCCGCCCGGCCGATGCCGAGGACCGGGAAACCTACCAGACCCGTTTTGCCGGCGAAGACGCCGCCAGCGTCGCCGCCCC
CACCGCCGGCCTCCACTTCACCCCCCGCCTGCTGGCGGAGGTCGAGGCTGCCGGCCTCAAGCGCGAAACCGTCCGCCTGC
ATGTGGGCCTGGGAACATTCAAACCGCTGGAAGACCGACACCTCAGCGAAAACCGTCTGCATGAGGAATGGCGCCGCATC
ACGCCGGAGGTGGCAGACCGCCTCAACGCCGCCCGTGCTGCGGGCGCCCGTCTTGTCCCGGTCGGCACCACAGCCATGCG
CACGCTGGAAAGCTGCGCGGGCGAAGACGGCCAGATCCACGCGGCCACCGGCCCCACCGACATCTTCCTCAAGCCCGGCG
ATCCCGTCCGCGCCACCGACGCGCTGATGACGAATTTTCACCTGCCGGGTTCCAGCCTCTTCATGCTCGTCAGCGCCCTG
ATGGGCACGAGCCTCATGCGCGCGGCCTACGCCCATGCCATCGAAGAAAAATACCGGTTCTATTCCTACGGCGACGCCTG
CCTGCTGCTGCCTTAA

Upstream 100 bases:

>100_bases
ACGAAGCCGCTGATGACGCTGCTCCAGAATCCGCCCCCGAATCCAACTCTGAAACCACCGGGGAATAGGGGGCATCGCCG
GCCTGATCCGGTGACCGTAC

Downstream 100 bases:

>100_bases
CGCGCCTCAGCGCGCATTCCGCCGCTGCCGCCCGAAGCTGAAGAAGCCGGCGAAAAACTCCGCCCACCACCGGCGCCAGC
GCCAGGCTTTGCGCAGGTGC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MDLSVFQFDLPEDLIALRPAQPQDAARLLVVHGDGRLIDATVRDLPKYLNTGDVLVFNDTRVLPAALKGVRPARDGASRD
VEVDVNLVERQGDCLWLALARPGRRLKDGDEIFFAEGFSARIASRREGGEILLDFNCEGEAMIAALDAHGAMPLPPYIAR
RRPADAEDRETYQTRFAGEDAASVAAPTAGLHFTPRLLAEVEAAGLKRETVRLHVGLGTFKPLEDRHLSENRLHEEWRRI
TPEVADRLNAARAAGARLVPVGTTAMRTLESCAGEDGQIHAATGPTDIFLKPGDPVRATDALMTNFHLPGSSLFMLVSAL
MGTSLMRAAYAHAIEEKYRFYSYGDACLLLP

Sequences:

>Translated_351_residues
MDLSVFQFDLPEDLIALRPAQPQDAARLLVVHGDGRLIDATVRDLPKYLNTGDVLVFNDTRVLPAALKGVRPARDGASRD
VEVDVNLVERQGDCLWLALARPGRRLKDGDEIFFAEGFSARIASRREGGEILLDFNCEGEAMIAALDAHGAMPLPPYIAR
RRPADAEDRETYQTRFAGEDAASVAAPTAGLHFTPRLLAEVEAAGLKRETVRLHVGLGTFKPLEDRHLSENRLHEEWRRI
TPEVADRLNAARAAGARLVPVGTTAMRTLESCAGEDGQIHAATGPTDIFLKPGDPVRATDALMTNFHLPGSSLFMLVSAL
MGTSLMRAAYAHAIEEKYRFYSYGDACLLLP
>Mature_351_residues
MDLSVFQFDLPEDLIALRPAQPQDAARLLVVHGDGRLIDATVRDLPKYLNTGDVLVFNDTRVLPAALKGVRPARDGASRD
VEVDVNLVERQGDCLWLALARPGRRLKDGDEIFFAEGFSARIASRREGGEILLDFNCEGEAMIAALDAHGAMPLPPYIAR
RRPADAEDRETYQTRFAGEDAASVAAPTAGLHFTPRLLAEVEAAGLKRETVRLHVGLGTFKPLEDRHLSENRLHEEWRRI
TPEVADRLNAARAAGARLVPVGTTAMRTLESCAGEDGQIHAATGPTDIFLKPGDPVRATDALMTNFHLPGSSLFMLVSAL
MGTSLMRAAYAHAIEEKYRFYSYGDACLLLP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=355, Percent_Identity=42.5352112676056, Blast_Score=231, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_HYPNA (Q0BYK7)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761436.1
- ProteinModelPortal:   Q0BYK7
- SMR:   Q0BYK7
- STRING:   Q0BYK7
- GeneID:   4288065
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2757
- NMPDR:   fig|228405.5.peg.2643
- TIGR:   HNE_2757
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   CYSHEIN
- BioCyc:   HNEP81032:HNE_2757-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38391; Mature: 38391

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSVFQFDLPEDLIALRPAQPQDAARLLVVHGDGRLIDATVRDLPKYLNTGDVLVFNDT
CCCCEEEECCCHHHHEECCCCCCCCEEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEECC
RVLPAALKGVRPARDGASRDVEVDVNLVERQGDCLWLALARPGRRLKDGDEIFFAEGFSA
CCHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCEEEEECCCHH
RIASRREGGEILLDFNCEGEAMIAALDAHGAMPLPPYIARRRPADAEDRETYQTRFAGED
HHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCC
AASVAAPTAGLHFTPRLLAEVEAAGLKRETVRLHVGLGTFKPLEDRHLSENRLHEEWRRI
HHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHC
TPEVADRLNAARAAGARLVPVGTTAMRTLESCAGEDGQIHAATGPTDIFLKPGDPVRATD
CHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCHHH
ALMTNFHLPGSSLFMLVSALMGTSLMRAAYAHAIEEKYRFYSYGDACLLLP
HHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEECC
>Mature Secondary Structure
MDLSVFQFDLPEDLIALRPAQPQDAARLLVVHGDGRLIDATVRDLPKYLNTGDVLVFNDT
CCCCEEEECCCHHHHEECCCCCCCCEEEEEEECCCEEEEHHHHHHHHHCCCCCEEEEECC
RVLPAALKGVRPARDGASRDVEVDVNLVERQGDCLWLALARPGRRLKDGDEIFFAEGFSA
CCHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCEEEEECCCHH
RIASRREGGEILLDFNCEGEAMIAALDAHGAMPLPPYIARRRPADAEDRETYQTRFAGED
HHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCC
AASVAAPTAGLHFTPRLLAEVEAAGLKRETVRLHVGLGTFKPLEDRHLSENRLHEEWRRI
HHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHC
TPEVADRLNAARAAGARLVPVGTTAMRTLESCAGEDGQIHAATGPTDIFLKPGDPVRATD
CHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCCHHH
ALMTNFHLPGSSLFMLVSALMGTSLMRAAYAHAIEEKYRFYSYGDACLLLP
HHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA