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The map label for this gene is 114797697

Identifier: 114797697

GI number: 114797697

Start: 84385

End: 85932

Strand: Reverse

Name: 114797697

Synonym: HNE_0096

Alternate gene names: NA

Gene position: 85932-84385 (Counterclockwise)

Preceding gene: 114798776

Following gene: 114798674

Centisome position: 2.32

GC content: 60.85

Gene sequence:

>1548_bases
ATGAGTTGGGAAAAATCGATTGAAGAGCTCCGCCGCCGGGAACGTCTGGCTGAGGAGATGGGGGGCGAGGAGCCGGTTTC
GCGACAGCGGGGCCGTGGCAAACTCACTGTGCGGGAAAGAGTGGCCTTCCTGGCTGACCCCGGCAGTTTCCACGAAATCG
GCAAGATCGCTGGCAAAGCGACCTATGGCGCGGATGAGGAACTGGACGGTTTCATGCCGTCCAATTCCGTCATGGGGCGG
GCGCGGCTGGATGGACATCCAGCAGTCATTCTGGCCGATGATTTCACGGTACGCGGCGGCGCCGCCGATGCCGCGATCTG
GCAAAAAATGGCCCAGGCTATCAAGATGGCTGCCGAGTACCGCATGCCGCTGGTTCAGATGATTGATGGCACCGGCGGGG
GCGGCTCTGTGAAGATGCTGGAGAAAGACCCGCGGACCTATATTCCCGAAACACCCGGCTGGAGCGAAATCGTTCATGGC
CTGACGCAGGTACCGTTCGTGTCCCTCGCCCTCGGACCTTGCGCAGGCATGGGAGCGGGCCGGGTCGCCGCCAGTCATTT
CAGCATCATGGTGAAAGAGCTCAGCCAGGTTTTTGTAGCCGGCCCGCCTGTTGCCATCGCGCTGGGCGAGAACGTCACCA
AGGAAGAACTTGGCGGCTGGAAAATCCAGGGGCAGAACGGCACCGTTGATAACGTCGTCGACAGCGAAGCCGACGCCTTC
ATCGCCGCGCGCCGGTTCCTGTCCTACCTGCCCCCTTCGGTGCATCATCTGCCTGGACGTATTCAGCCGGCAGACGATCC
GAAGCGGAAGGAAGAGAGCCTTCTTTCCATTGTGCCAAAAGATGGACGCACGCCCTACAAGCCCCGCCGTATCGTCGAAG
CGGTAGTGGACAAGGGCAGCTTCTTCGAAATTGGCCACGATTGGGGCCGGGGGATCGTAACGGGCCTCGCCCGGATTGAT
GGCCATGCCGTCGGTATTATGGCCGGTGACCCCTTCTTCCTGGATGGCGCCTGGACAGCGGATGTCTGTGACAAGGTCAC
CCGCCATATGGATCTCTGCTCGACGTTCCATCTGCCCGTGATCCATTTCGTGGATTGCCCCGGCTTTGCTGTTGGCGTGA
AAGCCGAAACCGCCGGTGTCACCCGCGCGGGCGTGCGGGCAATGACGGCAGTTTACCAGGCCAGCGTTCCGGTCTGCTCC
GTGATCATCCGCAAGGCATATGGGCTCGCTGGCTCTGCGATGATGAACCAGTCAAAGACCAAATGGCGCTATTGCTGGCC
AAGCGGCGACTGGGGCTCATTGCCGATGGCAGGCGGCATCGAGGCCGCTTTCCGCAAGGAACTCACCGAAGCCGAAGACC
CGGCCGCCCTGAAAGAGCAGCTCTACAAAAAGTTTGAAGCTATACGCTCACCCTTCCGGACGGCAGAAAGTTTCTTTGCG
GAGGAAATAATTGACCCGCGGGAGACCCGCCCCCTGCTCGTGGATTTCGTTCAGCATGCGCAGCGTATTGTCGAACCAGG
CGAAAGGCAAACCGGCTTCCGCCCCTGA

Upstream 100 bases:

>100_bases
CTCCTTGGATTGATACCTGTGGGGGATAAAGCAAACCGGACGATGCCCGGCAAGAAGATTCCCTTGACGCGCCTTGCGGC
ACATCGATCAACTGCAACGC

Downstream 100 bases:

>100_bases
CGGGAACGGAAACCATTGCGCTGAGTACAGCCCCCGCGAAGCAGGAATCAGGCGAGCACTTCTTTGGCCGCCGCATGGCA
TTTCTCGATTTCGTGATCGA

Product: carboxyl transferase domain-containing protein

Products: NA

Alternate protein names: Transcarboxylase 12S subunit [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSWEKSIEELRRRERLAEEMGGEEPVSRQRGRGKLTVRERVAFLADPGSFHEIGKIAGKATYGADEELDGFMPSNSVMGR
ARLDGHPAVILADDFTVRGGAADAAIWQKMAQAIKMAAEYRMPLVQMIDGTGGGGSVKMLEKDPRTYIPETPGWSEIVHG
LTQVPFVSLALGPCAGMGAGRVAASHFSIMVKELSQVFVAGPPVAIALGENVTKEELGGWKIQGQNGTVDNVVDSEADAF
IAARRFLSYLPPSVHHLPGRIQPADDPKRKEESLLSIVPKDGRTPYKPRRIVEAVVDKGSFFEIGHDWGRGIVTGLARID
GHAVGIMAGDPFFLDGAWTADVCDKVTRHMDLCSTFHLPVIHFVDCPGFAVGVKAETAGVTRAGVRAMTAVYQASVPVCS
VIIRKAYGLAGSAMMNQSKTKWRYCWPSGDWGSLPMAGGIEAAFRKELTEAEDPAALKEQLYKKFEAIRSPFRTAESFFA
EEIIDPRETRPLLVDFVQHAQRIVEPGERQTGFRP

Sequences:

>Translated_515_residues
MSWEKSIEELRRRERLAEEMGGEEPVSRQRGRGKLTVRERVAFLADPGSFHEIGKIAGKATYGADEELDGFMPSNSVMGR
ARLDGHPAVILADDFTVRGGAADAAIWQKMAQAIKMAAEYRMPLVQMIDGTGGGGSVKMLEKDPRTYIPETPGWSEIVHG
LTQVPFVSLALGPCAGMGAGRVAASHFSIMVKELSQVFVAGPPVAIALGENVTKEELGGWKIQGQNGTVDNVVDSEADAF
IAARRFLSYLPPSVHHLPGRIQPADDPKRKEESLLSIVPKDGRTPYKPRRIVEAVVDKGSFFEIGHDWGRGIVTGLARID
GHAVGIMAGDPFFLDGAWTADVCDKVTRHMDLCSTFHLPVIHFVDCPGFAVGVKAETAGVTRAGVRAMTAVYQASVPVCS
VIIRKAYGLAGSAMMNQSKTKWRYCWPSGDWGSLPMAGGIEAAFRKELTEAEDPAALKEQLYKKFEAIRSPFRTAESFFA
EEIIDPRETRPLLVDFVQHAQRIVEPGERQTGFRP
>Mature_514_residues
SWEKSIEELRRRERLAEEMGGEEPVSRQRGRGKLTVRERVAFLADPGSFHEIGKIAGKATYGADEELDGFMPSNSVMGRA
RLDGHPAVILADDFTVRGGAADAAIWQKMAQAIKMAAEYRMPLVQMIDGTGGGGSVKMLEKDPRTYIPETPGWSEIVHGL
TQVPFVSLALGPCAGMGAGRVAASHFSIMVKELSQVFVAGPPVAIALGENVTKEELGGWKIQGQNGTVDNVVDSEADAFI
AARRFLSYLPPSVHHLPGRIQPADDPKRKEESLLSIVPKDGRTPYKPRRIVEAVVDKGSFFEIGHDWGRGIVTGLARIDG
HAVGIMAGDPFFLDGAWTADVCDKVTRHMDLCSTFHLPVIHFVDCPGFAVGVKAETAGVTRAGVRAMTAVYQASVPVCSV
IIRKAYGLAGSAMMNQSKTKWRYCWPSGDWGSLPMAGGIEAAFRKELTEAEDPAALKEQLYKKFEAIRSPFRTAESFFAE
EIIDPRETRPLLVDFVQHAQRIVEPGERQTGFRP

Specific function: The 12S subunit specifically catalyzes the transfer of the carboxyl group of methylmalonyl CoA to the biotin of the 1.3S subunit forming propanoyl-CoA and carboxylated 1.3S-biotin [H]

COG id: COG4799

COG function: function code I; Acetyl-CoA carboxylase, carboxyltransferase component (subunits alpha and beta)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI119943100, Length=493, Percent_Identity=32.0486815415822, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI295821216, Length=513, Percent_Identity=31.5789473684211, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI11545863, Length=502, Percent_Identity=28.2868525896414, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI38679971, Length=326, Percent_Identity=24.5398773006135, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI38679977, Length=326, Percent_Identity=24.5398773006135, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI38679967, Length=326, Percent_Identity=24.5398773006135, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI38679960, Length=326, Percent_Identity=24.5398773006135, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI38679974, Length=326, Percent_Identity=24.5398773006135, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI134142062, Length=325, Percent_Identity=25.2307692307692, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25147359, Length=488, Percent_Identity=31.1475409836066, Blast_Score=234, Evalue=7e-62,
Organism=Caenorhabditis elegans, GI25147362, Length=488, Percent_Identity=31.1475409836066, Blast_Score=234, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17552936, Length=505, Percent_Identity=28.9108910891089, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71997163, Length=378, Percent_Identity=23.5449735449735, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24586065, Length=519, Percent_Identity=28.3236994219653, Blast_Score=188, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000438
- InterPro:   IPR000022
- InterPro:   IPR011763
- InterPro:   IPR011762 [H]

Pfam domain/function: PF01039 Carboxyl_trans [H]

EC number: =2.1.3.1 [H]

Molecular weight: Translated: 56175; Mature: 56044

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50980 COA_CT_NTER ; PS50989 COA_CT_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWEKSIEELRRRERLAEEMGGEEPVSRQRGRGKLTVRERVAFLADPGSFHEIGKIAGKA
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEHHHHHHHHCCCCCHHHHHHHHCCC
TYGADEELDGFMPSNSVMGRARLDGHPAVILADDFTVRGGAADAAIWQKMAQAIKMAAEY
CCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHH
RMPLVQMIDGTGGGGSVKMLEKDPRTYIPETPGWSEIVHGLTQVPFVSLALGPCAGMGAG
CCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCC
RVAASHFSIMVKELSQVFVAGPPVAIALGENVTKEELGGWKIQGQNGTVDNVVDSEADAF
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCEEEECCCCCHHHHHCCHHHHH
IAARRFLSYLPPSVHHLPGRIQPADDPKRKEESLLSIVPKDGRTPYKPRRIVEAVVDKGS
HHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
FFEIGHDWGRGIVTGLARIDGHAVGIMAGDPFFLDGAWTADVCDKVTRHMDLCSTFHLPV
EEECCHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHCCCC
IHFVDCPGFAVGVKAETAGVTRAGVRAMTAVYQASVPVCSVIIRKAYGLAGSAMMNQSKT
EEEECCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCC
KWRYCWPSGDWGSLPMAGGIEAAFRKELTEAEDPAALKEQLYKKFEAIRSPFRTAESFFA
CEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEIIDPRETRPLLVDFVQHAQRIVEPGERQTGFRP
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SWEKSIEELRRRERLAEEMGGEEPVSRQRGRGKLTVRERVAFLADPGSFHEIGKIAGKA
CHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEHHHHHHHHCCCCCHHHHHHHHCCC
TYGADEELDGFMPSNSVMGRARLDGHPAVILADDFTVRGGAADAAIWQKMAQAIKMAAEY
CCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCEEECCCCHHHHHHHHHHHHHHHHHHH
RMPLVQMIDGTGGGGSVKMLEKDPRTYIPETPGWSEIVHGLTQVPFVSLALGPCAGMGAG
CCCHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCC
RVAASHFSIMVKELSQVFVAGPPVAIALGENVTKEELGGWKIQGQNGTVDNVVDSEADAF
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCEEEECCCCCHHHHHCCHHHHH
IAARRFLSYLPPSVHHLPGRIQPADDPKRKEESLLSIVPKDGRTPYKPRRIVEAVVDKGS
HHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
FFEIGHDWGRGIVTGLARIDGHAVGIMAGDPFFLDGAWTADVCDKVTRHMDLCSTFHLPV
EEECCHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCHHHHHHHHHHHHHHHHHCCCC
IHFVDCPGFAVGVKAETAGVTRAGVRAMTAVYQASVPVCSVIIRKAYGLAGSAMMNQSKT
EEEECCCCEEEEEECHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCC
KWRYCWPSGDWGSLPMAGGIEAAFRKELTEAEDPAALKEQLYKKFEAIRSPFRTAESFFA
CEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEIIDPRETRPLLVDFVQHAQRIVEPGERQTGFRP
HHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366018 [H]