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The map label for this gene is lexA

Identifier: 114797550

GI number: 114797550

Start: 1830466

End: 1831149

Strand: Reverse

Name: lexA

Synonym: HNE_1786

Alternate gene names: 114797550

Gene position: 1831149-1830466 (Counterclockwise)

Preceding gene: 114800153

Following gene: 114798535

Centisome position: 49.42

GC content: 57.02

Gene sequence:

>684_bases
ATGCTCACAACAAAGCAAAAAGAGCTGCTGCTCTTCATTAATGATCGCATCAAGGATACCGGGGTTTCCCCCTCGTTTGA
CGAGATGAAGGAAGCGCTCGATCTGGCGTCAAAATCAGGTATCCACCGTTTGATTACGGCGCTTGAGGAGCGGGGCTTCA
TACGGCGCCTCGCAAATCGGGCGCGCGCGCTGGAAGTGCTGAAACTTCCAGATTCTGCCATTCCACCGCCGAACGCGCGT
CAAAGACGGGACTTTCGCCCAGCTCTGGTTACAAATCAGGGCGCTGAAGCCCCGCGTATTGCGGGTATGATTCCGCTTGT
CGGGCGCATCGCGGCGGGCTCTCCAATTTCCGCGATCCAGCAGGAGAACGGCCAGGTCGCCAGTCCTGGAGGATTGCCGG
AAGGCGACGATTATTTCGCGCTTGAAGTTCAAGGCGATTCGATGATTCAGGCCGGCATCCTGAATGGTGACACTGTGATC
CTGAAGCGCACGAACACCGCTCAAACCGGCGATATTGTTGTTGCGCTGATCGATGGCGAGGAGGCAACACTCAAGCGTCT
TCGCCGCAAAGGCGCATCTGTTGCCCTGGAAGCAGCAAACCCGGCATTCGAAACGCGCATCTTCGGGCCTGACAGGGTTG
AAGTTCAGGGCCGGCTGGTCGCGCTTATTCGCCGTTACGAATAA

Upstream 100 bases:

>100_bases
CGAGCAGGTTACCCGTCTGAAACTCGCCTGTTGAGATTGACCCGAACAGGGAACGCTTGTAGAACAAAAGCGAACATAGA
TTCGCATCGGGAACCGTTCC

Downstream 100 bases:

>100_bases
CGCGGTCAAAATACGCGATAGCGCCGCTTCTCGGGGCAAGAATGTCATCACTGCTGCACCGGCGCCAACGGCGTTCGGTG
CAGTTTTGTATGCGGACGAT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MLTTKQKELLLFINDRIKDTGVSPSFDEMKEALDLASKSGIHRLITALEERGFIRRLANRARALEVLKLPDSAIPPPNAR
QRRDFRPALVTNQGAEAPRIAGMIPLVGRIAAGSPISAIQQENGQVASPGGLPEGDDYFALEVQGDSMIQAGILNGDTVI
LKRTNTAQTGDIVVALIDGEEATLKRLRRKGASVALEAANPAFETRIFGPDRVEVQGRLVALIRRYE

Sequences:

>Translated_227_residues
MLTTKQKELLLFINDRIKDTGVSPSFDEMKEALDLASKSGIHRLITALEERGFIRRLANRARALEVLKLPDSAIPPPNAR
QRRDFRPALVTNQGAEAPRIAGMIPLVGRIAAGSPISAIQQENGQVASPGGLPEGDDYFALEVQGDSMIQAGILNGDTVI
LKRTNTAQTGDIVVALIDGEEATLKRLRRKGASVALEAANPAFETRIFGPDRVEVQGRLVALIRRYE
>Mature_227_residues
MLTTKQKELLLFINDRIKDTGVSPSFDEMKEALDLASKSGIHRLITALEERGFIRRLANRARALEVLKLPDSAIPPPNAR
QRRDFRPALVTNQGAEAPRIAGMIPLVGRIAAGSPISAIQQENGQVASPGGLPEGDDYFALEVQGDSMIQAGILNGDTVI
LKRTNTAQTGDIVVALIDGEEATLKRLRRKGASVALEAANPAFETRIFGPDRVEVQGRLVALIRRYE

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=203, Percent_Identity=29.064039408867, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_HYPNA (Q0C1A3)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760490.1
- ProteinModelPortal:   Q0C1A3
- SMR:   Q0C1A3
- STRING:   Q0C1A3
- MEROPS:   S24.001
- GeneID:   4287385
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1786
- NMPDR:   fig|228405.5.peg.1719
- TIGR:   HNE_1786
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   LMFIHER
- PhylomeDB:   Q0C1A3
- ProtClustDB:   PRK00215
- BioCyc:   HNEP81032:HNE_1786-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 24673; Mature: 24673

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: ACT_SITE 147-147 ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTKQKELLLFINDRIKDTGVSPSFDEMKEALDLASKSGIHRLITALEERGFIRRLANR
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ARALEVLKLPDSAIPPPNARQRRDFRPALVTNQGAEAPRIAGMIPLVGRIAAGSPISAIQ
HHHHHHEECCCCCCCCCCCHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHH
QENGQVASPGGLPEGDDYFALEVQGDSMIQAGILNGDTVILKRTNTAQTGDIVVALIDGE
HCCCCEECCCCCCCCCCEEEEEECCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEECCC
EATLKRLRRKGASVALEAANPAFETRIFGPDRVEVQGRLVALIRRYE
HHHHHHHHHCCCCEEEECCCCCCEEEECCCCCEEHHHHHHHHHHHCC
>Mature Secondary Structure
MLTTKQKELLLFINDRIKDTGVSPSFDEMKEALDLASKSGIHRLITALEERGFIRRLANR
CCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
ARALEVLKLPDSAIPPPNARQRRDFRPALVTNQGAEAPRIAGMIPLVGRIAAGSPISAIQ
HHHHHHEECCCCCCCCCCCHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHH
QENGQVASPGGLPEGDDYFALEVQGDSMIQAGILNGDTVILKRTNTAQTGDIVVALIDGE
HCCCCEECCCCCCCCCCEEEEEECCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEECCC
EATLKRLRRKGASVALEAANPAFETRIFGPDRVEVQGRLVALIRRYE
HHHHHHHHHCCCCEEEECCCCCCEEEECCCCCEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA