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The map label for this gene is hemL [H]

Identifier: 114797354

GI number: 114797354

Start: 2525547

End: 2526824

Strand: Reverse

Name: hemL [H]

Synonym: HNE_2418

Alternate gene names: 114797354

Gene position: 2526824-2525547 (Counterclockwise)

Preceding gene: 114799546

Following gene: 114799438

Centisome position: 68.2

GC content: 62.05

Gene sequence:

>1278_bases
ATGTGGACGAACTCAGTGGACAAGGCTTTGCGTGATCGTGCCGAAGCGGTCATTCCCGGCGGCATGTACGGCCACCAATC
CGTCCGCCTCCTGCCAGACGATTATCCCCAGTTCTTTGATCGCGCTTCGGGCGCCTATCTGTGGGATGCCGATGGCAACC
GGTACGTTGACTATATGTGCGGCTACGGCCCAAATCTCTTCGGCTATGGCCATGAAACCATTGATGCGGCCTACATCAAC
CAGCTGAAGAAAGGCGACACGATGACCGGCCCTGCCGGCCTCATGGTGGAACTCGCCGAGCAGATGACCGCGATGGTCTC
CCATGCCGATTGGGCCATGTTCTGCAAGAACGGCACGGACGCCACCACAATGGCCATGATGGTCGCCCGCAACTTCACCC
GCAAGAACACCATCGTCCTCGCCAAGGGCGCCTATCACGGCGCCGCCCCCTGGTGCACACCGGTGAAAAACGGCACCGCG
CCAAGCGACCGCGCCAATCAGGTCTTCTACACCTATAACGACGTCGCCAGCCTCGAAGAAGCTGTCCGCAAGGCCGGTGA
CGATCTCGCCGCCATCTTCGCCTCGCCCATCAAGCACGACACCTTCGTCGATCAGGAAGATCCGACAACGGAATATGCAC
GCCGCGCCCGCGAACTCTGCGATGAGAACGGCGCGCTGCTGATCGTCGATGATGTCCGCGCTGGTTTCCGCCTGGCCCGC
GATTCCAGCTGGGCGCGCGTCGGCGTGAAGCCGGATCTCTCCAGCTGGGGCAAGGCCATCGCAAATGGCCATCCCATCTC
TGCCTTGCTCGGTGCCGAAAAAGCCCGCAACGCCGCCGCCACGATTTATGCGACCGGCTCCTACTGGTTCTCCGCTGCGC
CGATGGCCGCAAGCCTTGAAACGATGCGCCTCATCCGCGAGACCGACTATCTTGAGCGCACCCAATCCCTCGGCCAGCGC
CTGCGCTCCGGGCTGGACGAAGCCGCAAACCGTCACGGCTTCGCCCTGCGCCAGACCGGCCCGGCCGAAATGCCGCTCAT
CATGTTCGAAGATGATCCGGGCTATTCCAAAGGCTACGCCTGGAACGATGCCCTGATGAAGCGCGGCGTCTACTTCCACC
CCTGGCACAACATGTTTATCTGCGCCGCCATGACCGAGGCGGACATCGACCACACGATTGAAGTCGCTGACGACGCCTTC
AAATTCGTGCGCGACGCGGGCACGCTGCCCCGTATCGAAAAACTGGCCATCCTCGGGCTGCTCACGGATGGTCACTGA

Upstream 100 bases:

>100_bases
TCAAGTTTCATTGTTTTCCCTCCCGAATTTACCTTGCGTCCATATCGGTCTTAAGTAAGATAGTCAAAACTATCTTATCG
GCAAAATCGGCGAGGGCAAC

Downstream 100 bases:

>100_bases
GGCAGCCGCGCCGGCGTCTCACTTCCAGATTGAGCATACAAGCACCTGGCATCCGTCTACGGATGAGGCCGGCCGGCTGA
CAATTGATATGGGCGCCATC

Product: glutamate-1-semialdehyde-2,1-aminomutase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MWTNSVDKALRDRAEAVIPGGMYGHQSVRLLPDDYPQFFDRASGAYLWDADGNRYVDYMCGYGPNLFGYGHETIDAAYIN
QLKKGDTMTGPAGLMVELAEQMTAMVSHADWAMFCKNGTDATTMAMMVARNFTRKNTIVLAKGAYHGAAPWCTPVKNGTA
PSDRANQVFYTYNDVASLEEAVRKAGDDLAAIFASPIKHDTFVDQEDPTTEYARRARELCDENGALLIVDDVRAGFRLAR
DSSWARVGVKPDLSSWGKAIANGHPISALLGAEKARNAAATIYATGSYWFSAAPMAASLETMRLIRETDYLERTQSLGQR
LRSGLDEAANRHGFALRQTGPAEMPLIMFEDDPGYSKGYAWNDALMKRGVYFHPWHNMFICAAMTEADIDHTIEVADDAF
KFVRDAGTLPRIEKLAILGLLTDGH

Sequences:

>Translated_425_residues
MWTNSVDKALRDRAEAVIPGGMYGHQSVRLLPDDYPQFFDRASGAYLWDADGNRYVDYMCGYGPNLFGYGHETIDAAYIN
QLKKGDTMTGPAGLMVELAEQMTAMVSHADWAMFCKNGTDATTMAMMVARNFTRKNTIVLAKGAYHGAAPWCTPVKNGTA
PSDRANQVFYTYNDVASLEEAVRKAGDDLAAIFASPIKHDTFVDQEDPTTEYARRARELCDENGALLIVDDVRAGFRLAR
DSSWARVGVKPDLSSWGKAIANGHPISALLGAEKARNAAATIYATGSYWFSAAPMAASLETMRLIRETDYLERTQSLGQR
LRSGLDEAANRHGFALRQTGPAEMPLIMFEDDPGYSKGYAWNDALMKRGVYFHPWHNMFICAAMTEADIDHTIEVADDAF
KFVRDAGTLPRIEKLAILGLLTDGH
>Mature_425_residues
MWTNSVDKALRDRAEAVIPGGMYGHQSVRLLPDDYPQFFDRASGAYLWDADGNRYVDYMCGYGPNLFGYGHETIDAAYIN
QLKKGDTMTGPAGLMVELAEQMTAMVSHADWAMFCKNGTDATTMAMMVARNFTRKNTIVLAKGAYHGAAPWCTPVKNGTA
PSDRANQVFYTYNDVASLEEAVRKAGDDLAAIFASPIKHDTFVDQEDPTTEYARRARELCDENGALLIVDDVRAGFRLAR
DSSWARVGVKPDLSSWGKAIANGHPISALLGAEKARNAAATIYATGSYWFSAAPMAASLETMRLIRETDYLERTQSLGQR
LRSGLDEAANRHGFALRQTGPAEMPLIMFEDDPGYSKGYAWNDALMKRGVYFHPWHNMFICAAMTEADIDHTIEVADDAF
KFVRDAGTLPRIEKLAILGLLTDGH

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786349, Length=427, Percent_Identity=31.8501170960187, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789016, Length=350, Percent_Identity=26.5714285714286, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788044, Length=312, Percent_Identity=26.6025641025641, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1789759, Length=322, Percent_Identity=27.3291925465839, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787560, Length=318, Percent_Identity=27.0440251572327, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI145693181, Length=316, Percent_Identity=25.9493670886076, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21356575, Length=335, Percent_Identity=24.1791044776119, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46905; Mature: 46905

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTNSVDKALRDRAEAVIPGGMYGHQSVRLLPDDYPQFFDRASGAYLWDADGNRYVDYMC
CCCCHHHHHHHHHHHEECCCCCCCCCEEEECCCCCHHHHHCCCCCEEECCCCCEEEEEEE
GYGPNLFGYGHETIDAAYINQLKKGDTMTGPAGLMVELAEQMTAMVSHADWAMFCKNGTD
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ATTMAMMVARNFTRKNTIVLAKGAYHGAAPWCTPVKNGTAPSDRANQVFYTYNDVASLEE
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHH
AVRKAGDDLAAIFASPIKHDTFVDQEDPTTEYARRARELCDENGALLIVDDVRAGFRLAR
HHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEECC
DSSWARVGVKPDLSSWGKAIANGHPISALLGAEKARNAAATIYATGSYWFSAAPMAASLE
CCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEECCCHHHHHH
TMRLIRETDYLERTQSLGQRLRSGLDEAANRHGFALRQTGPAEMPLIMFEDDPGYSKGYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCCCCCCE
WNDALMKRGVYFHPWHNMFICAAMTEADIDHTIEVADDAFKFVRDAGTLPRIEKLAILGL
ECHHHHHCCCEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHEEEEE
LTDGH
ECCCC
>Mature Secondary Structure
MWTNSVDKALRDRAEAVIPGGMYGHQSVRLLPDDYPQFFDRASGAYLWDADGNRYVDYMC
CCCCHHHHHHHHHHHEECCCCCCCCCEEEECCCCCHHHHHCCCCCEEECCCCCEEEEEEE
GYGPNLFGYGHETIDAAYINQLKKGDTMTGPAGLMVELAEQMTAMVSHADWAMFCKNGTD
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ATTMAMMVARNFTRKNTIVLAKGAYHGAAPWCTPVKNGTAPSDRANQVFYTYNDVASLEE
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHH
AVRKAGDDLAAIFASPIKHDTFVDQEDPTTEYARRARELCDENGALLIVDDVRAGFRLAR
HHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEECC
DSSWARVGVKPDLSSWGKAIANGHPISALLGAEKARNAAATIYATGSYWFSAAPMAASLE
CCCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEECCCHHHHHH
TMRLIRETDYLERTQSLGQRLRSGLDEAANRHGFALRQTGPAEMPLIMFEDDPGYSKGYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCCCCCCE
WNDALMKRGVYFHPWHNMFICAAMTEADIDHTIEVADDAFKFVRDAGTLPRIEKLAILGL
ECHHHHHCCCEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHEEEEE
LTDGH
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA