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The map label for this gene is tig

Identifier: 114797334

GI number: 114797334

Start: 2142130

End: 2143467

Strand: Reverse

Name: tig

Synonym: HNE_2069

Alternate gene names: 114797334

Gene position: 2143467-2142130 (Counterclockwise)

Preceding gene: 114800405

Following gene: 114800125

Centisome position: 57.85

GC content: 60.61

Gene sequence:

>1338_bases
ATGGAAGTGACACAGACCAAGGCAGAAGGTCTTAGCCGCACCTTTGCGGTCAAGGTGCCGGTCAGCGAGCTGCAGGCGAA
ACTCGATGCCCGCATCGAGGAAATCCGCCCGCAAATGCGCCTCAAGGGCTTCCGCCCTGGCAAGGTGCCCGCAGCTCATG
TGCGCAAGATGTATGGCCGCGATCTCATGGGCGAAGTGATCGACAAGCTGGTCAACGAGACCAACCAGAAGGCGCTCGAA
GAAAACTCGCTGCGCCCGGCAGGCACGCCGAATGTCGACATGCAGGCCGACATCGAGAAAGTCGTCACCGGCAAGGAAGA
CCTTTCCTACCAGATGCACGTCGATGTCATGCCGGAGTTCACACCGGTGGATGTGTCCACGCTGACCATTGTGCGTCCGG
TTGCTGAAATCGCAGACGAACAGATCGACGAAGCCCTCAAGCGCATCGCCGACCAGAACATGAAGTACGAGCCCCGCGCG
GAAGGCGAAGCCTCCCAGGACGGCGACGCCGTGATCGTCGACTTCGTCGGCAAGATCGACGGTGAAGCCTTCGAAGGTGG
CACGGCTGAGCAACAGTCGGTCGTCCTCGGCGCTAACCGCTTCATTCCCGGCTTTGAGGAACAACTGCTCGGCGTGAAAG
CTGGCGAAGAGAAAGAACTCAACGTTTCCTTCCCGGAAGACTACCCGGCAGCAAACCTCGCCGGCAAAGCCGCTGTCTTC
GAAACCAAGATCCACGAAGTCCGCGCCCCGCAGACGCCCGAGATGGACGATGAGTTCGCCAAGGGACTCGGCCTGGAAAG
CCTGGAACAGCTGCGCGGCCTGGTGAAAGACCAGCTGGCCAACGAGCACACCACCGCCTCGCGTTCCAAAGCCAAGCGCG
ACCTGCTCGACAAGCTCGATGCGGCGCATGATTTCGATCTGCCGCCCGGCATGGTCGATCAGGAGTTCGAGCAGATCTGG
CAACAGCTGCAGCGTGAAATGGACGCTGGCCGCGTTTCCGATGAGGAAAAAGCGAAGCCGGAAGAAACCCTGCGCGATGA
ATACCGCAAGATCGCCGAGCGCCGCGTGCGCCTTGGTCTCGTGCTGGCCGAAGTCGGCCGCATCGCCGATGTCCGCATCT
CCGAGGCTGAAGTGAACCAGGCCCTGATCCGCGAAGCCCGCCAGTATCCGGGCCAGGAGCGGGAAGTCGTCCAGTTCTTC
CAGAAGAACCCCAACGCGATGGCTCAGCTGCGCGCGCCGATCTATGAGGACAAGGTCGTCGACCACATCCTCGAAACGGC
AAAGGTTGAGGAAAAGACCGTCACCCGCGAAGAACTTTTCGCCGAAGACGAAGAATAA

Upstream 100 bases:

>100_bases
CTTATGGCTGTGACATAAGCCGCCGCTTCAGGCAGGTACAGCAGGCTCCGGGCGCGCATCTGGCGTGCGCCGCGCCGCCC
AATAAACGGAAGAATGTCCC

Downstream 100 bases:

>100_bases
GCCTTTTCCTTACAAGGCAATTCAAGCCGGCCGGTCGAAAGACCGGCCGGTTTTGTTTTGGTGGGGTTCTTTCCATCCCG
TCATTTTGGTAAATTCCTGT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MEVTQTKAEGLSRTFAVKVPVSELQAKLDARIEEIRPQMRLKGFRPGKVPAAHVRKMYGRDLMGEVIDKLVNETNQKALE
ENSLRPAGTPNVDMQADIEKVVTGKEDLSYQMHVDVMPEFTPVDVSTLTIVRPVAEIADEQIDEALKRIADQNMKYEPRA
EGEASQDGDAVIVDFVGKIDGEAFEGGTAEQQSVVLGANRFIPGFEEQLLGVKAGEEKELNVSFPEDYPAANLAGKAAVF
ETKIHEVRAPQTPEMDDEFAKGLGLESLEQLRGLVKDQLANEHTTASRSKAKRDLLDKLDAAHDFDLPPGMVDQEFEQIW
QQLQREMDAGRVSDEEKAKPEETLRDEYRKIAERRVRLGLVLAEVGRIADVRISEAEVNQALIREARQYPGQEREVVQFF
QKNPNAMAQLRAPIYEDKVVDHILETAKVEEKTVTREELFAEDEE

Sequences:

>Translated_445_residues
MEVTQTKAEGLSRTFAVKVPVSELQAKLDARIEEIRPQMRLKGFRPGKVPAAHVRKMYGRDLMGEVIDKLVNETNQKALE
ENSLRPAGTPNVDMQADIEKVVTGKEDLSYQMHVDVMPEFTPVDVSTLTIVRPVAEIADEQIDEALKRIADQNMKYEPRA
EGEASQDGDAVIVDFVGKIDGEAFEGGTAEQQSVVLGANRFIPGFEEQLLGVKAGEEKELNVSFPEDYPAANLAGKAAVF
ETKIHEVRAPQTPEMDDEFAKGLGLESLEQLRGLVKDQLANEHTTASRSKAKRDLLDKLDAAHDFDLPPGMVDQEFEQIW
QQLQREMDAGRVSDEEKAKPEETLRDEYRKIAERRVRLGLVLAEVGRIADVRISEAEVNQALIREARQYPGQEREVVQFF
QKNPNAMAQLRAPIYEDKVVDHILETAKVEEKTVTREELFAEDEE
>Mature_445_residues
MEVTQTKAEGLSRTFAVKVPVSELQAKLDARIEEIRPQMRLKGFRPGKVPAAHVRKMYGRDLMGEVIDKLVNETNQKALE
ENSLRPAGTPNVDMQADIEKVVTGKEDLSYQMHVDVMPEFTPVDVSTLTIVRPVAEIADEQIDEALKRIADQNMKYEPRA
EGEASQDGDAVIVDFVGKIDGEAFEGGTAEQQSVVLGANRFIPGFEEQLLGVKAGEEKELNVSFPEDYPAANLAGKAAVF
ETKIHEVRAPQTPEMDDEFAKGLGLESLEQLRGLVKDQLANEHTTASRSKAKRDLLDKLDAAHDFDLPPGMVDQEFEQIW
QQLQREMDAGRVSDEEKAKPEETLRDEYRKIAERRVRLGLVLAEVGRIADVRISEAEVNQALIREARQYPGQEREVVQFF
QKNPNAMAQLRAPIYEDKVVDHILETAKVEEKTVTREELFAEDEE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=446, Percent_Identity=31.8385650224215, Blast_Score=211, Evalue=9e-56,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_HYPNA (Q0C0H6)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760767.1
- ProteinModelPortal:   Q0C0H6
- SMR:   Q0C0H6
- STRING:   Q0C0H6
- GeneID:   4289412
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2069
- NMPDR:   fig|228405.5.peg.1995
- TIGR:   HNE_2069
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q0C0H6
- ProtClustDB:   PRK01490
- BioCyc:   HNEP81032:HNE_2069-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49976; Mature: 49976

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVTQTKAEGLSRTFAVKVPVSELQAKLDARIEEIRPQMRLKGFRPGKVPAAHVRKMYGR
CCCCCCHHHCCCCEEEEECCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHCH
DLMGEVIDKLVNETNQKALEENSLRPAGTPNVDMQADIEKVVTGKEDLSYQMHVDVMPEF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHCCEEEEEEECCCC
TPVDVSTLTIVRPVAEIADEQIDEALKRIADQNMKYEPRAEGEASQDGDAVIVDFVGKID
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHCC
GEAFEGGTAEQQSVVLGANRFIPGFEEQLLGVKAGEEKELNVSFPEDYPAANLAGKAAVF
CCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
ETKIHEVRAPQTPEMDDEFAKGLGLESLEQLRGLVKDQLANEHTTASRSKAKRDLLDKLD
HHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
AAHDFDLPPGMVDQEFEQIWQQLQREMDAGRVSDEEKAKPEETLRDEYRKIAERRVRLGL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
VLAEVGRIADVRISEAEVNQALIREARQYPGQEREVVQFFQKNPNAMAQLRAPIYEDKVV
HHHHHCCHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCHHHHHHH
DHILETAKVEEKTVTREELFAEDEE
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEVTQTKAEGLSRTFAVKVPVSELQAKLDARIEEIRPQMRLKGFRPGKVPAAHVRKMYGR
CCCCCCHHHCCCCEEEEECCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCHHHHHHHHCH
DLMGEVIDKLVNETNQKALEENSLRPAGTPNVDMQADIEKVVTGKEDLSYQMHVDVMPEF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHCCEEEEEEECCCC
TPVDVSTLTIVRPVAEIADEQIDEALKRIADQNMKYEPRAEGEASQDGDAVIVDFVGKID
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHCC
GEAFEGGTAEQQSVVLGANRFIPGFEEQLLGVKAGEEKELNVSFPEDYPAANLAGKAAVF
CCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
ETKIHEVRAPQTPEMDDEFAKGLGLESLEQLRGLVKDQLANEHTTASRSKAKRDLLDKLD
HHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
AAHDFDLPPGMVDQEFEQIWQQLQREMDAGRVSDEEKAKPEETLRDEYRKIAERRVRLGL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
VLAEVGRIADVRISEAEVNQALIREARQYPGQEREVVQFFQKNPNAMAQLRAPIYEDKVV
HHHHHCCHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHCCHHHHHHH
DHILETAKVEEKTVTREELFAEDEE
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA