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The map label for this gene is fixI [H]

Identifier: 114797319

GI number: 114797319

Start: 1246335

End: 1248554

Strand: Reverse

Name: fixI [H]

Synonym: HNE_1226

Alternate gene names: 114797319

Gene position: 1248554-1246335 (Counterclockwise)

Preceding gene: 114798267

Following gene: 114800454

Centisome position: 33.7

GC content: 66.31

Gene sequence:

>2220_bases
ATGACCGGCCTCGCCCTCGACACCAATGGCTGCCCCAGCGGGCTCGCGCCGCCGAGCGGGTCAGATACCTCTGACCCGGC
CGCCTTCGTGCGCAAGGTAGGCAAGCAAAGCCGCCTGTCGCTCACGGTGCGCGGGGCGAAATGCGGCGGGTGTCTCTCGC
GTATCGAGGGGGCGGTTTCTGCATTACCGGGCGTAGAGAGCGCAAGACTGAACCTTTCAAGCGGAAAGCTGGAGATTGCA
TGGTCGGGCAATCTGGAACCCCGGCGGATAAGCGAAGCGGTGAGCAGCCTTGGATATGGCGTGGCGCCGCTGAATACGGC
CACGGAAGATGATGGGCACAAGAAGGAGGAGCAATCGCTTCTCCTCGCCATGGGCGTTGCGGGTTTTGCGACGGCGAACA
TCATGCTGCTGTCTGTCTCCGTGTGGGGCGGGCATGAGGAAATGGGCGAGGCGACGCGGCAGGGCCTGCATGCGCTTTCC
GGCGTGATTGCCTTGCCGACGCTGATTTATTCGGGGCGGCCATTCTTCCGGTCTGCGTGGAATGTTCTGAAGAAGCGGCG
CACGAATATGGATGTGCCGATCTCGCTGGCGCTGATCCTCGCTTTCTCGGTCAGCGTGGCGGAGACGCTGCGCGGCGGAG
AACACGCGTATTTTGATGCGGCGTGCATGTTGTTGTTCTTCCTGCTGATCGGGCGGTTCCTGGATGCGCGGGTGCGGCGG
CGGGCCTGGTCGGCGGCGCGCGATCTTGCTGCGCTGGCCAGCCGCACGGTGATGCGGATCGGCGCCGATGGCGCGCCGGT
ATCTGCGCGCGCGGAGGACATTCGCCCCGGCGACAGCATCCTTCTGGCGCCGGGCGAGCGCGCGGTGGTGGACATGGTGA
TTGAGGGCGGGGAGTCGGAAGTAGACGAGAGCCTTGTGACGGGCGAGAGCCTGCCGCGCTGGACGGGCACGGGCATGAAG
CTGATGGCGGGCGCGATCAACCTCTCCCAGCCGCTGACCGGGCGGGCGACGGCGGCAGCGGCAGACTCCCTTCTGGCCGA
AATCAGCCAGATGCTGACAGCGGGCGAGCAGAAGCGGTCGGCCTATCGCCAGATCGCTGACAAGGCGGTGTCGCTTTATG
TGCCGCTGGTGCATTCGACGGCATTGCTGACGTTTCTGGGTTGGTGGCTGGTGGGTGGCATGGGATTGCGGGAGGCGATC
CTGGTTGCTGTCTCTACGCTGATCATCACCTGTCCTTGCGCGTTGGCGCTGGCGGCGCCGGTTGTGCAGGTCGTGGCGGC
GGGGCGGTTGTTCCGGGGCGGGGCTTATCTCAAATCGGGTGACGCGCTGGAGCGGATTGCGGCGTGTGATCATGTGATCT
TTGACAAGACCGGTACGCTGACCCTTGGCACGCCGCGCCTTGTTTCGACGGGCATTCCCGCCGCGCTGATTGGCGACGCG
GCGCAGCTGGCGCGGGCGAGCCGGCATCCGCTGTCGCGGGCTTTGACGGCGGCCGCTGGGCCTGGGCCATTGGGAACGGA
TGTGAAGGAGCGGCCCGGTCTTGGGCTTGAAGGAATGGTCGGCGGCGTGGCCTGCCGGCTGGGCTCTGCTGAATGGACGG
GCGCGGCGGCGACGGGGCATGTGGGCGCGACGCTGTTCTTTGCGCGGGGCGAAGAGGCGCCGGTGGAGCTGCGCTTTGAG
GATGATATTCAGGGCGACGCGGCCGGGATGCTGGCGAAGCTTTCGGCCATGGGGATGACGATGGAGATCGTCTCGGGCGA
CCGGGCAGAAGCTGTGGCGGAAGTGGCGCGGGCGCTGGGGATTGATCAGTGGACGGCGGGCGCCAGTCCGCAGGACAAGG
TGGCGCGGCTGGAGGCGTTGCAGGCGCAGGGGCGCAAGGTGTTCATGGTGGGCGATGGGCTCAATGATGCGGGCGCGTTG
GCGCTGGCACATGCATCGGCGGCGCCCGGCGGGGCGCTGGATGTGAGCCAGTCTGCGGCGGATGCGGTGTATTCAGGCGG
GCTTGCGCCCCTGCCCCGGCTGGTGAAGACGGCGCGGACGGCGAAGGCGGTGATGTTGCAGAATTTCGGATTTGCGGCGG
CGTATAATCTGGTGGCGGTGCCGATCGCGGTTCTGGGACATGCGACACCGCTGGTGGCGGCGATTGCGATGTCGGGGTCT
TCGATTGTGGTGTGCCTGAACGCGCTGCGGATCAATCTGAATACGGAGAAGGCGGTATGA

Upstream 100 bases:

>100_bases
GCGACCTGCATGCAGGCGCCTGGAGCGTCAATATTGCGGCCGATGTCGATCCGGAAACGGATGGCTTCGAGTTCCGCGCC
AGCCGGGAAATCATTGTGCC

Downstream 100 bases:

>100_bases
GCGGTCTTGTGTTCCTGATACCCATCGCCCTGATGATGGGCGGCCTGGGGCTGGTCGGGTTTCTCTGGGCGGTGCGGACC
GGCCAGTTTGACGATCCTGA

Product: copper-translocating P-type ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 739; Mature: 738

Protein sequence:

>739_residues
MTGLALDTNGCPSGLAPPSGSDTSDPAAFVRKVGKQSRLSLTVRGAKCGGCLSRIEGAVSALPGVESARLNLSSGKLEIA
WSGNLEPRRISEAVSSLGYGVAPLNTATEDDGHKKEEQSLLLAMGVAGFATANIMLLSVSVWGGHEEMGEATRQGLHALS
GVIALPTLIYSGRPFFRSAWNVLKKRRTNMDVPISLALILAFSVSVAETLRGGEHAYFDAACMLLFFLLIGRFLDARVRR
RAWSAARDLAALASRTVMRIGADGAPVSARAEDIRPGDSILLAPGERAVVDMVIEGGESEVDESLVTGESLPRWTGTGMK
LMAGAINLSQPLTGRATAAAADSLLAEISQMLTAGEQKRSAYRQIADKAVSLYVPLVHSTALLTFLGWWLVGGMGLREAI
LVAVSTLIITCPCALALAAPVVQVVAAGRLFRGGAYLKSGDALERIAACDHVIFDKTGTLTLGTPRLVSTGIPAALIGDA
AQLARASRHPLSRALTAAAGPGPLGTDVKERPGLGLEGMVGGVACRLGSAEWTGAAATGHVGATLFFARGEEAPVELRFE
DDIQGDAAGMLAKLSAMGMTMEIVSGDRAEAVAEVARALGIDQWTAGASPQDKVARLEALQAQGRKVFMVGDGLNDAGAL
ALAHASAAPGGALDVSQSAADAVYSGGLAPLPRLVKTARTAKAVMLQNFGFAAAYNLVAVPIAVLGHATPLVAAIAMSGS
SIVVCLNALRINLNTEKAV

Sequences:

>Translated_739_residues
MTGLALDTNGCPSGLAPPSGSDTSDPAAFVRKVGKQSRLSLTVRGAKCGGCLSRIEGAVSALPGVESARLNLSSGKLEIA
WSGNLEPRRISEAVSSLGYGVAPLNTATEDDGHKKEEQSLLLAMGVAGFATANIMLLSVSVWGGHEEMGEATRQGLHALS
GVIALPTLIYSGRPFFRSAWNVLKKRRTNMDVPISLALILAFSVSVAETLRGGEHAYFDAACMLLFFLLIGRFLDARVRR
RAWSAARDLAALASRTVMRIGADGAPVSARAEDIRPGDSILLAPGERAVVDMVIEGGESEVDESLVTGESLPRWTGTGMK
LMAGAINLSQPLTGRATAAAADSLLAEISQMLTAGEQKRSAYRQIADKAVSLYVPLVHSTALLTFLGWWLVGGMGLREAI
LVAVSTLIITCPCALALAAPVVQVVAAGRLFRGGAYLKSGDALERIAACDHVIFDKTGTLTLGTPRLVSTGIPAALIGDA
AQLARASRHPLSRALTAAAGPGPLGTDVKERPGLGLEGMVGGVACRLGSAEWTGAAATGHVGATLFFARGEEAPVELRFE
DDIQGDAAGMLAKLSAMGMTMEIVSGDRAEAVAEVARALGIDQWTAGASPQDKVARLEALQAQGRKVFMVGDGLNDAGAL
ALAHASAAPGGALDVSQSAADAVYSGGLAPLPRLVKTARTAKAVMLQNFGFAAAYNLVAVPIAVLGHATPLVAAIAMSGS
SIVVCLNALRINLNTEKAV
>Mature_738_residues
TGLALDTNGCPSGLAPPSGSDTSDPAAFVRKVGKQSRLSLTVRGAKCGGCLSRIEGAVSALPGVESARLNLSSGKLEIAW
SGNLEPRRISEAVSSLGYGVAPLNTATEDDGHKKEEQSLLLAMGVAGFATANIMLLSVSVWGGHEEMGEATRQGLHALSG
VIALPTLIYSGRPFFRSAWNVLKKRRTNMDVPISLALILAFSVSVAETLRGGEHAYFDAACMLLFFLLIGRFLDARVRRR
AWSAARDLAALASRTVMRIGADGAPVSARAEDIRPGDSILLAPGERAVVDMVIEGGESEVDESLVTGESLPRWTGTGMKL
MAGAINLSQPLTGRATAAAADSLLAEISQMLTAGEQKRSAYRQIADKAVSLYVPLVHSTALLTFLGWWLVGGMGLREAIL
VAVSTLIITCPCALALAAPVVQVVAAGRLFRGGAYLKSGDALERIAACDHVIFDKTGTLTLGTPRLVSTGIPAALIGDAA
QLARASRHPLSRALTAAAGPGPLGTDVKERPGLGLEGMVGGVACRLGSAEWTGAAATGHVGATLFFARGEEAPVELRFED
DIQGDAAGMLAKLSAMGMTMEIVSGDRAEAVAEVARALGIDQWTAGASPQDKVARLEALQAQGRKVFMVGDGLNDAGALA
LAHASAAPGGALDVSQSAADAVYSGGLAPLPRLVKTARTAKAVMLQNFGFAAAYNLVAVPIAVLGHATPLVAAIAMSGSS
IVVCLNALRINLNTEKAV

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=784, Percent_Identity=26.9132653061224, Blast_Score=229, Evalue=9e-60,
Organism=Homo sapiens, GI115529486, Length=575, Percent_Identity=27.1304347826087, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI55743073, Length=520, Percent_Identity=27.5, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI1786691, Length=735, Percent_Identity=31.4285714285714, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1789879, Length=714, Percent_Identity=28.1512605042017, Blast_Score=196, Evalue=5e-51,
Organism=Escherichia coli, GI1786914, Length=503, Percent_Identity=28.2306163021869, Blast_Score=99, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17556548, Length=475, Percent_Identity=27.7894736842105, Blast_Score=166, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6319772, Length=601, Percent_Identity=25.7903494176373, Blast_Score=173, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6320475, Length=626, Percent_Identity=24.7603833865815, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI221329854, Length=520, Percent_Identity=27.1153846153846, Blast_Score=161, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 76274; Mature: 76143

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00059 ADH_ZINC ; PS00154 ATPASE_E1_E2 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLALDTNGCPSGLAPPSGSDTSDPAAFVRKVGKQSRLSLTVRGAKCGGCLSRIEGAVS
CCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHH
ALPGVESARLNLSSGKLEIAWSGNLEPRRISEAVSSLGYGVAPLNTATEDDGHKKEEQSL
HCCCCCHHEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
LLAMGVAGFATANIMLLSVSVWGGHEEMGEATRQGLHALSGVIALPTLIYSGRPFFRSAW
HHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NVLKKRRTNMDVPISLALILAFSVSVAETLRGGEHAYFDAACMLLFFLLIGRFLDARVRR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAWSAARDLAALASRTVMRIGADGAPVSARAEDIRPGDSILLAPGERAVVDMVIEGGESE
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHCCCCCCCEEECCCCCEEEEEEECCCCHH
VDESLVTGESLPRWTGTGMKLMAGAINLSQPLTGRATAAAADSLLAEISQMLTAGEQKRS
HHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
AYRQIADKAVSLYVPLVHSTALLTFLGWWLVGGMGLREAILVAVSTLIITCPCALALAAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VVQVVAAGRLFRGGAYLKSGDALERIAACDHVIFDKTGTLTLGTPRLVSTGIPAALIGDA
HHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHEECCCCCEEECCCHHHHCCCCHHHHHHH
AQLARASRHPLSRALTAAAGPGPLGTDVKERPGLGLEGMVGGVACRLGSAEWTGAAATGH
HHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
VGATLFFARGEEAPVELRFEDDIQGDAAGMLAKLSAMGMTMEIVSGDRAEAVAEVARALG
CCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHC
IDQWTAGASPQDKVARLEALQAQGRKVFMVGDGLNDAGALALAHASAAPGGALDVSQSAA
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHH
DAVYSGGLAPLPRLVKTARTAKAVMLQNFGFAAAYNLVAVPIAVLGHATPLVAAIAMSGS
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
SIVVCLNALRINLNTEKAV
EEEEEEEEHEEECCHHCCC
>Mature Secondary Structure 
TGLALDTNGCPSGLAPPSGSDTSDPAAFVRKVGKQSRLSLTVRGAKCGGCLSRIEGAVS
CCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHH
ALPGVESARLNLSSGKLEIAWSGNLEPRRISEAVSSLGYGVAPLNTATEDDGHKKEEQSL
HCCCCCHHEEEECCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
LLAMGVAGFATANIMLLSVSVWGGHEEMGEATRQGLHALSGVIALPTLIYSGRPFFRSAW
HHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
NVLKKRRTNMDVPISLALILAFSVSVAETLRGGEHAYFDAACMLLFFLLIGRFLDARVRR
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RAWSAARDLAALASRTVMRIGADGAPVSARAEDIRPGDSILLAPGERAVVDMVIEGGESE
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHCCCCCCCEEECCCCCEEEEEEECCCCHH
VDESLVTGESLPRWTGTGMKLMAGAINLSQPLTGRATAAAADSLLAEISQMLTAGEQKRS
HHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
AYRQIADKAVSLYVPLVHSTALLTFLGWWLVGGMGLREAILVAVSTLIITCPCALALAAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VVQVVAAGRLFRGGAYLKSGDALERIAACDHVIFDKTGTLTLGTPRLVSTGIPAALIGDA
HHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHEECCCCCEEECCCHHHHCCCCHHHHHHH
AQLARASRHPLSRALTAAAGPGPLGTDVKERPGLGLEGMVGGVACRLGSAEWTGAAATGH
HHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
VGATLFFARGEEAPVELRFEDDIQGDAAGMLAKLSAMGMTMEIVSGDRAEAVAEVARALG
CCEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHC
IDQWTAGASPQDKVARLEALQAQGRKVFMVGDGLNDAGALALAHASAAPGGALDVSQSAA
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCHHHH
DAVYSGGLAPLPRLVKTARTAKAVMLQNFGFAAAYNLVAVPIAVLGHATPLVAAIAMSGS
HHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
SIVVCLNALRINLNTEKAV
EEEEEEEEHEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8661920; 12597275 [H]