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The map label for this gene is purF [H]

Identifier: 114797301

GI number: 114797301

Start: 729490

End: 730941

Strand: Reverse

Name: purF [H]

Synonym: HNE_0717

Alternate gene names: 114797301

Gene position: 730941-729490 (Counterclockwise)

Preceding gene: 114798030

Following gene: 114800463

Centisome position: 19.73

GC content: 61.57

Gene sequence:

>1452_bases
ATGGTCTTTTTCGATCATGATGACGATAAACCCCACGAAGAATGTGGAGTTTTCGGAGTCTTTGGCAGCCTTGAAGCAAG
TCTGCTGACAGCCCTGGGGCTTCATGCCCTTCAACACCGAGGACAGGAAGCGGCCGGCATCGTCAGCTATGACGGCAAGC
GCTTCTCCTCCGAGCGCCATATGGGCCTTGTCGGTGAGAGTTTCGGCGGAGACCTTCGCCAGCGTCTTCCCGGCCATGCC
GCCATCGGCCACAACCGATACTCCACGCAGGGCCGCCCGATGGCCCGCAACATCCAGCCGATCTTTGCAGACCTTGATAC
CGGCGGCTTCGCCGTGGCGCATAACGGCAACCTGACCAATGCCCGCATCCTGCGCCAGGAACTGGTGCGCAATGGCGCGA
TCTTCCAGTCCACGATGGACACCGAAGTGATCCTGCACCTGGTCGCCCGCAGCCCGAAAAAGAAGTTCGTCGAGCGCCTC
GTCGATGCGATGCACCAGATCGAAGGCGGCTACGCTCTCGTCGGCATTACCGGCAAGAAGCTGATCGGCGCGCGCGACCC
CATCGGCCTGCGTCCGTTGATCCTCGGCCGTCATGGCAAATCCTATGTGCTCGCCTCGGAAACCTGCGCGCTCGATATTA
TCGGCGCAGAATTTGTCCGCGAGATCGAGAATGGCGAAGTGGTCGTCATCAGTGAGGAAGGCATCGAATCGATCCGCGCC
TTCCCGCCGCGCCCGCCCAGCCCGTGCATTTTCGAGTATGTCTACTTCGCCCGCCCGGACAGCTTCATTCAGGGCCGCTC
GGTTTATGAAGTGCGCCGCCGCATGGGCAACCAGCTTGCACTCGAAACGCATGCCGACGCCGATGTGATCGTGCCTGTGC
CAGACTCCGGCGTGCCCGCCGCGCTCGGCTTTTCCGAGGCCTCCGGCATTCCCTTCCAGATGGGCATCATCCGGAACCAT
TATGTCGGCCGGACTTTCATCCAGCCCACCCATACGGGCCGCCAGACCGCTATCTCCAAAAAGCACAGCCCCAACCGCGC
CGTGCTTGAGGGCAAGCGTGTGATCCTGGTGGACGATTCCATCGTGCGCGGCAACACGTCGAAGAAAATCGTGCAGATGG
TGCGCGAAGCCGGCGCCCGCGAGATCCACTTCCGGTCGGCCAGCCCGCCGATCGTCAACCCGGATTTCTACGGCATCGAC
ATGGCCGCGAAATCGGAACTCTTCGCGGCCACCCATACGCATGAAGAGATGGTCCGTGAGCTGAAGGTGGAAAGCCTCGG
CTTCCTGTCTGTCCCCGGCCTCTACAAAGCTATTGGCGAGCCGGTCCGCAATGGCATGCAGCCGCAATTTGCCGACCATT
GCTTCACCGGCGACTACCCGACCCAGCTGCTCGACCATCAGCGCGCCCAGGCAGACAAAGAGCGTCAGCTCTCCCTGCTG
GACGATGTGTGA

Upstream 100 bases:

>100_bases
TTGATGACAGGAATGTCACTGGCGGGCTTTCGCCGGAAGTCGAACCGGCCTATATCCCGGCCTTAGGCATAGGCCCCCAC
CAGCAAAGCCGGAACGTCCG

Downstream 100 bases:

>100_bases
GCCCCGCGCTAAAGCCGCGCGATGACCTCTACACCTCGCCTTGTTCTCGTTACCGGCGCCTCGCGCGGCATCGGCCGCGC
CATCAGCCTTGAGCTTGCCC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MVFFDHDDDKPHEECGVFGVFGSLEASLLTALGLHALQHRGQEAAGIVSYDGKRFSSERHMGLVGESFGGDLRQRLPGHA
AIGHNRYSTQGRPMARNIQPIFADLDTGGFAVAHNGNLTNARILRQELVRNGAIFQSTMDTEVILHLVARSPKKKFVERL
VDAMHQIEGGYALVGITGKKLIGARDPIGLRPLILGRHGKSYVLASETCALDIIGAEFVREIENGEVVVISEEGIESIRA
FPPRPPSPCIFEYVYFARPDSFIQGRSVYEVRRRMGNQLALETHADADVIVPVPDSGVPAALGFSEASGIPFQMGIIRNH
YVGRTFIQPTHTGRQTAISKKHSPNRAVLEGKRVILVDDSIVRGNTSKKIVQMVREAGAREIHFRSASPPIVNPDFYGID
MAAKSELFAATHTHEEMVRELKVESLGFLSVPGLYKAIGEPVRNGMQPQFADHCFTGDYPTQLLDHQRAQADKERQLSLL
DDV

Sequences:

>Translated_483_residues
MVFFDHDDDKPHEECGVFGVFGSLEASLLTALGLHALQHRGQEAAGIVSYDGKRFSSERHMGLVGESFGGDLRQRLPGHA
AIGHNRYSTQGRPMARNIQPIFADLDTGGFAVAHNGNLTNARILRQELVRNGAIFQSTMDTEVILHLVARSPKKKFVERL
VDAMHQIEGGYALVGITGKKLIGARDPIGLRPLILGRHGKSYVLASETCALDIIGAEFVREIENGEVVVISEEGIESIRA
FPPRPPSPCIFEYVYFARPDSFIQGRSVYEVRRRMGNQLALETHADADVIVPVPDSGVPAALGFSEASGIPFQMGIIRNH
YVGRTFIQPTHTGRQTAISKKHSPNRAVLEGKRVILVDDSIVRGNTSKKIVQMVREAGAREIHFRSASPPIVNPDFYGID
MAAKSELFAATHTHEEMVRELKVESLGFLSVPGLYKAIGEPVRNGMQPQFADHCFTGDYPTQLLDHQRAQADKERQLSLL
DDV
>Mature_483_residues
MVFFDHDDDKPHEECGVFGVFGSLEASLLTALGLHALQHRGQEAAGIVSYDGKRFSSERHMGLVGESFGGDLRQRLPGHA
AIGHNRYSTQGRPMARNIQPIFADLDTGGFAVAHNGNLTNARILRQELVRNGAIFQSTMDTEVILHLVARSPKKKFVERL
VDAMHQIEGGYALVGITGKKLIGARDPIGLRPLILGRHGKSYVLASETCALDIIGAEFVREIENGEVVVISEEGIESIRA
FPPRPPSPCIFEYVYFARPDSFIQGRSVYEVRRRMGNQLALETHADADVIVPVPDSGVPAALGFSEASGIPFQMGIIRNH
YVGRTFIQPTHTGRQTAISKKHSPNRAVLEGKRVILVDDSIVRGNTSKKIVQMVREAGAREIHFRSASPPIVNPDFYGID
MAAKSELFAATHTHEEMVRELKVESLGFLSVPGLYKAIGEPVRNGMQPQFADHCFTGDYPTQLLDHQRAQADKERQLSLL
DDV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=506, Percent_Identity=38.1422924901186, Blast_Score=333, Evalue=3e-91,
Organism=Homo sapiens, GI4826742, Length=210, Percent_Identity=31.9047619047619, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788651, Length=475, Percent_Identity=37.8947368421053, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI1790167, Length=227, Percent_Identity=28.6343612334802, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=38.0753138075314, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17539970, Length=223, Percent_Identity=27.8026905829596, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17532897, Length=216, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17532899, Length=216, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323958, Length=494, Percent_Identity=34.0080971659919, Blast_Score=249, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6322745, Length=233, Percent_Identity=28.3261802575107, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24659598, Length=486, Percent_Identity=38.0658436213992, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI28573187, Length=491, Percent_Identity=38.0855397148676, Blast_Score=315, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24659604, Length=465, Percent_Identity=38.494623655914, Blast_Score=313, Evalue=1e-85,
Organism=Drosophila melanogaster, GI21357745, Length=183, Percent_Identity=30.0546448087432, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53183; Mature: 53183

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFFDHDDDKPHEECGVFGVFGSLEASLLTALGLHALQHRGQEAAGIVSYDGKRFSSERH
CEEECCCCCCCHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
MGLVGESFGGDLRQRLPGHAAIGHNRYSTQGRPMARNIQPIFADLDTGGFAVAHNGNLTN
CCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCH
ARILRQELVRNGAIFQSTMDTEVILHLVARSPKKKFVERLVDAMHQIEGGYALVGITGKK
HHHHHHHHHHCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCH
LIGARDPIGLRPLILGRHGKSYVLASETCALDIIGAEFVREIENGEVVVISEEGIESIRA
HCCCCCCCCCCEEEEECCCCCEEEECCCCEEEHHHHHHHHHHCCCCEEEEECCCHHHHHC
FPPRPPSPCIFEYVYFARPDSFIQGRSVYEVRRRMGNQLALETHADADVIVPVPDSGVPA
CCCCCCCCHHHHHHHHCCCCHHHCCCHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCC
ALGFSEASGIPFQMGIIRNHYVGRTFIQPTHTGRQTAISKKHSPNRAVLEGKRVILVDDS
EECCCCCCCCCEEECHHHCCCCCCEEECCCCCCCHHHHHCCCCCCHHEECCCEEEEECCC
IVRGNTSKKIVQMVREAGAREIHFRSASPPIVNPDFYGIDMAAKSELFAATHTHEEMVRE
EECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHCCHHHHHHCCHHHHHHH
LKVESLGFLSVPGLYKAIGEPVRNGMQPQFADHCFTGDYPTQLLDHQRAQADKERQLSLL
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
DDV
CCC
>Mature Secondary Structure
MVFFDHDDDKPHEECGVFGVFGSLEASLLTALGLHALQHRGQEAAGIVSYDGKRFSSERH
CEEECCCCCCCHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
MGLVGESFGGDLRQRLPGHAAIGHNRYSTQGRPMARNIQPIFADLDTGGFAVAHNGNLTN
CCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCH
ARILRQELVRNGAIFQSTMDTEVILHLVARSPKKKFVERLVDAMHQIEGGYALVGITGKK
HHHHHHHHHHCCCEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCH
LIGARDPIGLRPLILGRHGKSYVLASETCALDIIGAEFVREIENGEVVVISEEGIESIRA
HCCCCCCCCCCEEEEECCCCCEEEECCCCEEEHHHHHHHHHHCCCCEEEEECCCHHHHHC
FPPRPPSPCIFEYVYFARPDSFIQGRSVYEVRRRMGNQLALETHADADVIVPVPDSGVPA
CCCCCCCCHHHHHHHHCCCCHHHCCCHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCCC
ALGFSEASGIPFQMGIIRNHYVGRTFIQPTHTGRQTAISKKHSPNRAVLEGKRVILVDDS
EECCCCCCCCCEEECHHHCCCCCCEEECCCCCCCHHHHHCCCCCCHHEECCCEEEEECCC
IVRGNTSKKIVQMVREAGAREIHFRSASPPIVNPDFYGIDMAAKSELFAATHTHEEMVRE
EECCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHCCHHHHHHCCHHHHHHH
LKVESLGFLSVPGLYKAIGEPVRNGMQPQFADHCFTGDYPTQLLDHQRAQADKERQLSLL
HHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHH
DDV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA